Determination of the viability of Toxoplasma gondii oocysts by PCR real-time after treatment with propidium monoazide

November 12, 2020
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RNase H-dependent PCR enables highly specific amplification of antibody variable domains from single B-cells

 

Immunization-based antibody discovery platforms require robust and effective protocols for the amplification, cloning, expression, and screening of antibodies from large numbers of B-cells in order to effectively capture the diversity of an experienced Ig-repertoire. Multiplex PCR using a series of forward and reverse primers designed to recover antibodies from a range of different germline sequences is challenging because primer design requires the recovery of full length antibody sequences, low starting template concentrations, and the need for all the primers to function under the same PCR conditions.
Here we demonstrate several advantages to incorporating RNase H2-dependent PCR (rh-PCR) into a high-throughput, antibody-discovery platform. Firstly, rh-PCR eliminated primer dimer synthesis to below detectable levels, thereby eliminating clones with a false positive antibody titer. Secondly, by increasing the specificity of PCR, the rh-PCR primers increased the recovery of cognate antibody variable regions from single B-cells, as well as downstream recombinant antibody titers.
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Monkeypox Virus Real Time PCR Kit
ZD-0076-01 25 tests/kit Ask for price
Description: Monkeypox virus is the virus that causes the disease monkeypox in both humans and animals. Monkeypox virus is an Orthopoxvirus, a genus of the family Poxviridae that contains other viral species that target mammals. The virus is mainly found in tropical rainforest regions of central and West Africa. The primary route of infection is thought to be contact with the infected animals or their bodily fluids. The genome is not segmented and contains a single molecule of linear double-stranded DNA, 185000 nucleotides long. The Monkeypox Virus real time PCR Kit contains a specific ready-to-use system for the detection of the Monkeypox Virusthrough polymerase chain reaction (PCR) in the real-time PCR system. The master contains reagents and enzymes for the specific amplification of the Monkeypox Virus DNA. Fluorescence is emitted and measured by the real time systems ́ optical unit during the PCR. The detection of amplified Monkeypox Virus DNA fragment is performed in fluorimeter channel 530nm with the fluorescent quencher BHQ1. DNA extraction buffer is available in the kit and serum or lesion exudate samples are used for the extraction of the DNA. In addition, the kit contains a system to identify possible PCR inhibition by measuring the 560nm fluorescence of the internal control (IC). An external positive control defined as 1×10^7 copies/ml is supplied which allow the determination of the gene load.

Monkeypox Virus Real Time PCR Kit
ZD-0076-02 25 tests/kit Ask for price
Description: Monkeypox virus is the virus that causes the disease monkeypox in both humans and animals. Monkeypox virus is an Orthopoxvirus, a genus of the family Poxviridae that contains other viral species that target mammals. The virus is mainly found in tropical rainforest regions of central and West Africa. The primary route of infection is thought to be contact with the infected animals or their bodily fluids.The genome is not segmented and contains a single molecule of linear double-stranded DNA, 185000 nucleotides long.The Monkeypox Virus real time PCR Kit contains a specific ready-to-use system for the detection of the Monkeypox Virusthrough polymerase chain reaction (PCR) in the real-time PCR system. The master contains reagents and enzymes for the specific amplification of theMonkeypox VirusDNA. Fluorescence is emitted and measured by the real time systems ́ optical unit during the PCR. The detection of amplified Monkeypox Virus DNA fragment is performed in fluorimeter channelFAM with the fluorescent quencher BHQ1. DNA extraction buffer is available in the kit and serum or lesion exudate samples are used for the extraction of the DNA. In addition, the kit contains a system to identify possible PCR inhibition by measuring the HEX/VIC/JOE fluorescence of the internal control (IC). An external positive control defined as 1×107copies/ml is supplied which allow the determination of the gene load.

MDV Real-Time PCR Kit
PD65-06 96t
EUR 1864.32
Description: Please check the datasheet of MDV Real-Time PCR Kit before using the test.

ALV Real-Time PCR Kit
PD65-15 96t
EUR 2069.28
Description: Please check the datasheet of ALV Real-Time PCR Kit before using the test.

hsa-mir-521 Real-time RT-PCR Detection Kit
20-abx097600
  • EUR 661.20
  • EUR 944.40
  • EUR 477.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

lyophilised real-time PCR Master Mix for dual labeled probes
M-PCR-156L 960 reactions x 20 µl
EUR 900.68
Description: lyophilised real-time PCR Master Mix for dual labeled probes

lyophilised real-time PCR Master Mix for dual labeled probes
M-PCR-156S 192 reactions x 20 µl
EUR 225.3
Description: lyophilised real-time PCR Master Mix for dual labeled probes

Novel Coronavirus COVID-19 (2019-nCoV) Real Time RT-PCR Kit
RR-0478-02 25 tests/kit
EUR 1189.2
Description: Novel Coronavirus (2019-nCoV) Real Time RT-PCR Kit is used for the qualitative detection of a novel coronavirus, which was identified in 2019 at Wuhan City, Hubei Province, China, in upper respiratory tract specimens (nasopharyngeal extracts, deep cough sputum, etc.) and lower respiratory tract specimens (alveoli irrigation fluid, etc.) by real time PCR systems.

Rubella virus One-Step PCR kit
Oneq-H481-100R 100T
EUR 1932

Rubella virus One-Step PCR kit
Oneq-H481-150R 150T
EUR 2646

Rubella virus One-Step PCR kit
Oneq-H481-50R 50T
EUR 1410

0,1ML THIN WALL TUBES 8 PER STRIP. CLEAR & REAL TIME STRIP CAPS
PCR-0108-LP-RT-C 125/pk
EUR 850.8
Description: PCR Plates & Tubes; PCR Strip Tubes and Strip Caps - Axygen

0,1ML THIN WALL TUBES 8 PER STRIP. WHITE & REAL TIME STRIP CAPS
PCR-0108-LP-RT-W 125/pk
EUR 924
Description: PCR Plates & Tubes; PCR Strip Tubes and Strip Caps - Axygen

hsa-mir-521 Real-Time RT-PCR Detection and U6 Calibration Kit
20-abx097601
  • EUR 878.40
  • EUR 1245.60
  • EUR 627.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

TurboQ Real-time PCR Machine with Laptop
MOL2700 EACH
EUR 30805.08

lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain
M-PCR-173L 960 reactions x 20 µl
EUR 789.36
Description: lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain

lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain
M-PCR-173S 192 reactions x 20 µl
EUR 197.34
Description: lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain

Virus Zika RT PCR kit
RTq-H434-100R 100T
EUR 1573.2

Virus Zika RT PCR kit
RTq-H434-150R 150T
EUR 2144.4

Virus Zika RT PCR kit
RTq-H434-50R 50T
EUR 1155.6

Dengue virus RT PCR kit
RTq-H451-100R 100T
EUR 1573.2

Dengue virus RT PCR kit
RTq-H451-150R 150T
EUR 2144.4

Dengue virus RT PCR kit
RTq-H451-50R 50T
EUR 1155.6

Chikungunya virus RT PCR kit
RTq-H525-100R 100T
EUR 1573.2

Chikungunya virus RT PCR kit
RTq-H525-150R 150T
EUR 2144.4

Chikungunya virus RT PCR kit
RTq-H525-50R 50T
EUR 1155.6

Measles Virus RT PCR kit
RTq-H639-100R 100T
EUR 1573.2

Measles Virus RT PCR kit
RTq-H639-150R 150T
EUR 2144.4

Measles Virus RT PCR kit
RTq-H639-50R 50T
EUR 1155.6

Mumps Virus RT PCR kit
RTq-H640-100R 100T
EUR 1573.2

Mumps Virus RT PCR kit
RTq-H640-150R 150T
EUR 2144.4

Mumps Virus RT PCR kit
RTq-H640-50R 50T
EUR 1155.6

Uukuniemi virus RT PCR kit
RTq-V056-100R 100T
EUR 1573.2

Uukuniemi virus RT PCR kit
RTq-V056-150R 150T
EUR 2144.4

Uukuniemi virus RT PCR kit
RTq-V056-50R 50T
EUR 946.8

Reticuloendotheliosis virus RT PCR kit
RTq-V061-100R 100T
EUR 1573.2

Reticuloendotheliosis virus RT PCR kit
RTq-V061-150R 150T
EUR 2144.4

Reticuloendotheliosis virus RT PCR kit
RTq-V061-50R 50T
EUR 1155.6

Distemper virus RT PCR kit
RTq-V111-100R 100T
EUR 1573.2

Distemper virus RT PCR kit
RTq-V111-150R 150T
EUR 2144.4

Distemper virus RT PCR kit
RTq-V111-50R 50T
EUR 1155.6

Pseudorabies Virus RT PCR kit
RTq-V123-100D 100T
EUR 860.4

Pseudorabies Virus RT PCR kit
RTq-V123-150D 150T
EUR 969.6

Pseudorabies Virus RT PCR kit
RTq-V123-50D 50T
EUR 717.6

Cowpox virus RT PCR kit
RTq-V173-100D 100T
EUR 860.4

Cowpox virus RT PCR kit
RTq-V173-150D 150T
EUR 969.6

Cowpox virus RT PCR kit
RTq-V173-50D 50T
EUR 717.6

Akabane virus RT PCR kit
RTq-V267-100R 100T
EUR 860.4

Akabane virus RT PCR kit
RTq-V267-150R 150T
EUR 2144.4

Akabane virus RT PCR kit
RTq-V267-50R 50T
EUR 1155.6

Irido virus RT PCR kit
RTq-V288-100D 100T
EUR 860.4

Irido virus RT PCR kit
RTq-V288-150D 150T
EUR 969.6

Irido virus RT PCR kit
RTq-V288-50D 50T
EUR 717.6

Mourilyan virus RT PCR kit
RTq-V291-100R 100T
EUR 1573.2

Mourilyan virus RT PCR kit
RTq-V291-150R 150T
EUR 2144.4

Mourilyan virus RT PCR kit
RTq-V291-50R 50T
EUR 1155.6

Schmallenberg virus RT PCR kit
RTq-V319-100R 100T
EUR 1573.2

Schmallenberg virus RT PCR kit
RTq-V319-150R 150T
EUR 2144.4

Schmallenberg virus RT PCR kit
RTq-V319-50R 50T
EUR 1155.6

Ectromelia virus RT PCR kit
RTq-V330-100D 100T
EUR 860.4

Ectromelia virus RT PCR kit
RTq-V330-150D 150T
EUR 969.6

Ectromelia virus RT PCR kit
RTq-V330-50D 50T
EUR 717.6

K Virus RT PCR kit
RTq-V365-100D 100T
EUR 860.4

K Virus RT PCR kit
RTq-V365-150D 150T
EUR 969.6

K Virus RT PCR kit
RTq-V365-50D 50T
EUR 717.6

Sialodacryoadenitis virus RT PCR kit
RTq-V370-100R 100T
EUR 1573.2

Sialodacryoadenitis virus RT PCR kit
RTq-V370-150R 150T
EUR 2144.4

Sialodacryoadenitis virus RT PCR kit
RTq-V370-50R 50T
EUR 1155.6

Sendai virus RT PCR kit
RTq-V374-100R 100T
EUR 1573.2

Sendai virus RT PCR kit
RTq-V374-150R 150T
EUR 2144.4

Sendai virus RT PCR kit
RTq-V374-50R 50T
EUR 1155.6

Junin Virus RT PCR kit
RTq-V388-100R 100T
EUR 1573.2

Junin Virus RT PCR kit
RTq-V388-150R 150T
EUR 2144.4

Junin Virus RT PCR kit
RTq-V388-50R 50T
EUR 1155.6

Machupo Virus RT PCR kit
RTq-V389-100R 100T
EUR 1573.2

Machupo Virus RT PCR kit
RTq-V389-150R 150T
EUR 2144.4

Machupo Virus RT PCR kit
RTq-V389-50R 50T
EUR 1155.6

Lassa virus RT PCR kit
RTq-V390-100R 100T
EUR 1573.2

Lassa virus RT PCR kit
RTq-V390-150R 150T
EUR 2144.4

Lassa virus RT PCR kit
RTq-V390-50R 50T
EUR 1155.6

Guanarito virus RT PCR kit
RTq-V391-100R 100T
EUR 1573.2

Guanarito virus RT PCR kit
RTq-V391-150R 150T
EUR 2144.4

Guanarito virus RT PCR kit
RTq-V391-50R 50T
EUR 1155.6

Sabia Virus RT PCR kit
RTq-V392-100R 100T
EUR 1573.2

Sabia Virus RT PCR kit
RTq-V392-150R 150T
EUR 2144.4

Sabia Virus RT PCR kit
RTq-V392-50R 50T
EUR 1155.6

Ecephalomyocarditis virus RT PCR kit
RTq-V394-100R 100T
EUR 1573.2

Ecephalomyocarditis virus RT PCR kit
RTq-V394-150R 150T
EUR 2144.4

Ecephalomyocarditis virus RT PCR kit
RTq-V394-50R 50T
EUR 1155.6

Visna virus RT PCR kit
RTq-V634-100R 100T
EUR 1573.2

Visna virus RT PCR kit
RTq-V634-150R 150T
EUR 2144.4

Visna virus RT PCR kit
RTq-V634-50R 50T
EUR 1155.6

Swinepox virus RT PCR kit
RTq-V727-100D 100T
EUR 860.4

Swinepox virus RT PCR kit
RTq-V727-150D 150T
EUR 969.6

Swinepox virus RT PCR kit
RTq-V727-50D 50T
EUR 717.6

Kemerovo virus RT PCR kit
RTq-VH057-100R 100T
EUR 1573.2

Kemerovo virus RT PCR kit
RTq-VH057-150R 150T
EUR 2144.4

Kemerovo virus RT PCR kit
RTq-VH057-50R 50T
EUR 946.8

Lipovnik virus RT PCR kit
RTq-VH058-100R 100T
EUR 1573.2

Lipovnik virus RT PCR kit
RTq-VH058-150R 150T
EUR 2144.4

Lipovnik virus RT PCR kit
RTq-VH058-50R 50T
EUR 1155.6

Rabies Virus RT PCR kit
RTq-VH113-100R 100T
EUR 1573.2

Rabies Virus RT PCR kit
RTq-VH113-150R 150T
EUR 2144.4

Rabies Virus RT PCR kit
RTq-VH113-50R 50T
EUR 1155.6

Nipah virus RT PCR kit
RTq-VH139-100R 100T
EUR 1573.2

Nipah virus RT PCR kit
RTq-VH139-150R 150T
EUR 2144.4

Nipah virus RT PCR kit
RTq-VH139-50R 50T
EUR 1155.6

Norwalk virus RT PCR kit
RTq-VH262-100R 100T
EUR 1573.2

Norwalk virus RT PCR kit
RTq-VH262-150R 150T
EUR 2144.4

Norwalk virus RT PCR kit
RTq-VH262-50R 50T
EUR 1155.6

Hanta Virus RT PCR kit
RTq-VH373-100R 100T
EUR 1573.2

Hanta Virus RT PCR kit
RTq-VH373-150R 150T
EUR 2144.4

Hanta Virus RT PCR kit
RTq-VH373-50R 50T
EUR 1155.6

Seoul virus RT PCR kit
RTq-VH395-100R 100T
EUR 1573.2

Seoul virus RT PCR kit
RTq-VH395-150R 150T
EUR 2144.4

Seoul virus RT PCR kit
RTq-VH395-50R 50T
EUR 1155.6

Akabane virus PCR kit
PCR-V267-48R 50T
EUR 987.6

Akabane virus PCR kit
PCR-V267-96R 100T
EUR 1335.6

Irido virus PCR kit
PCR-V288-48D 50T
EUR 543.6

Irido virus PCR kit
PCR-V288-96D 100T
EUR 686.4

Mourilyan virus PCR kit
PCR-V291-48R 50T
EUR 987.6

Mourilyan virus PCR kit
PCR-V291-96R 100T
EUR 1335.6

Schmallenberg virus PCR kit
PCR-V319-48R 50T
EUR 813.6

Schmallenberg virus PCR kit
PCR-V319-96R 100T
EUR 1335.6

Ectromelia virus PCR kit
PCR-V330-48D 50T
EUR 543.6

Ectromelia virus PCR kit
PCR-V330-96D 100T
EUR 686.4

K Virus PCR kit
PCR-V365-48D 50T
EUR 543.6

K Virus PCR kit
PCR-V365-96D 100T
EUR 686.4

Sialodacryoadenitis virus PCR kit
PCR-V370-48R 50T
EUR 987.6

Sialodacryoadenitis virus PCR kit
PCR-V370-96R 100T
EUR 1335.6

Sendai virus PCR kit
PCR-V374-48R 50T
EUR 987.6

Sendai virus PCR kit
PCR-V374-96R 100T
EUR 1335.6

Junin Virus PCR kit
PCR-V388-48R 50T
EUR 987.6

Junin Virus PCR kit
PCR-V388-96R 100T
EUR 1335.6

Machupo Virus PCR kit
PCR-V389-48R 50T
EUR 987.6

Machupo Virus PCR kit
PCR-V389-96R 100T
EUR 1335.6

Lassa virus PCR kit
PCR-V390-48R 50T
EUR 813.6

Lassa virus PCR kit
PCR-V390-96R 100T
EUR 1335.6

Guanarito virus PCR kit
PCR-V391-48R 50T
EUR 987.6

Guanarito virus PCR kit
PCR-V391-96R 100T
EUR 1335.6

Sabia Virus PCR kit
PCR-V392-48R 50T
EUR 987.6

Sabia Virus PCR kit
PCR-V392-96R 100T
EUR 1335.6

Ecephalomyocarditis virus PCR kit
PCR-V394-48R 50T
EUR 987.6

Ecephalomyocarditis virus PCR kit
PCR-V394-96R 100T
EUR 1335.6

Visna virus PCR kit
PCR-V634-48R 50T
EUR 987.6

Visna virus PCR kit
PCR-V634-96R 100T
EUR 1335.6

Swinepox virus PCR kit
PCR-V727-48D 50T
EUR 543.6

Swinepox virus PCR kit
PCR-V727-96D 100T
EUR 686.4

Kemerovo virus PCR kit
PCR-VH057-48R 50T
EUR 987.6

Kemerovo virus PCR kit
PCR-VH057-96R 100T
EUR 1335.6

Lipovnik virus PCR kit
PCR-VH058-48R 50T
EUR 987.6

Lipovnik virus PCR kit
PCR-VH058-96R 100T
EUR 1335.6

Rabies Virus PCR kit
PCR-VH113-48R 50T
EUR 987.6

Rabies Virus PCR kit
PCR-VH113-96R 100T
EUR 1335.6

Nipah virus PCR kit
PCR-VH139-48R 50T
EUR 987.6

Nipah virus PCR kit
PCR-VH139-96R 100T
EUR 1335.6

Norwalk virus PCR kit
PCR-VH262-48R 50T
EUR 987.6

Norwalk virus PCR kit
PCR-VH262-96R 100T
EUR 1335.6
Finally, we demonstrate that rh-PCR primers provide a more homogeneous sample pool and greater sequence quality in a Next Generation Sequencing-based approach to obtaining DNA sequence information from large numbers of cloned antibody cognate pairs. Furthermore, the higher specificity of the rh-PCR primers allowed for a better match between native antibody germline sequences and the VL/VH fragments amplified from single B-cells.

Determination of the viability of Toxoplasma gondii oocysts by PCR real-time after treatment with propidium monoazide

 

This study aimed to investigate a methodology for discriminating viable and non-viable T. gondii oocysts in water. Analyses included two steps: (i) microscopic investigation with vital dyes; (ii) molecular investigation, using a real time PCR (qPCR), after parasite treatment (or not) with propidium monoazide (PMA).
The method was called qPCR-PMA. Oocyst aliquots were incubated (15 min) at 25 ºC or 100 ºC and analyzed by microscopy, after trypan blue and neutral red staining. Microscopic investigation determined viable and non-viable oocysts. For the molecular investigation, both aliquots of oocysts were treated with PMA. Non-viable oocysts, after PMA treatment, exhibited an inhibition of DNA amplification by qPCR. Although analyses were carried out with oocysts treated experimentally, these results suggest that qPCR-PMA can be a useful strategy to distinguish viable and non-viable T. gondii oocysts in water safety testing, showing if water is safe to drink.

Diagnostic Utility of a Custom 34-Gene Anchored Multiplex PCR-Based Next-Generation Sequencing Fusion Panel for the Diagnosis of Bone and Soft Tissue Neoplasms With Identification of Novel USP6 Fusion Partners in Aneurysmal Bone Cysts

 

Context.—: Bone and soft tissue tumors are heterogeneous, diagnostically challenging, and often defined by gene fusions.
Objective.—: To present our experience using a custom 34-gene targeted sequencing fusion panel.
Design.—: Total nucleic acid extracted from formalin-fixed, paraffin-embedded (FFPE) tumor specimens was subjected to open-ended, nested anchored multiplex polymerase chain reaction and enrichment of 34 gene targets, thus enabling detection of known and novel fusion partners.
Results.—: During a 12-month period, 145 patients were tested as part of routine clinical care. Tumor percentage ranged from 10% to 100% and turnaround time ranged from 3 to 15 (median, 7.9) days. The most common diagnostic groups were small round blue cell tumors, tumors of uncertain differentiation, fibroblastic/myofibroblastic tumors, and adipocytic tumors.
In-frame fusion transcripts were identified in 64 of 140 cases sequenced (46%): in 62 cases, the detection of a disease-defining fusion confirmed the morphologic impression; in 2 cases, a germline TFG-GPR128 polymorphic fusion variant was detected. Several genes in the panel partnered with multiple fusion partners specific for different diagnoses, for example, EWSR1, NR4A3, FUS, NCOA2, and TFE3. Interesting examples are presented to highlight how fusion detection or lack thereof was instrumental in establishing accurate diagnoses. Novel fusion partners were detected for 2 cases of solid aneurysmal bone cysts (PTBP1-USP6, SLC38A2-USP6).
Conclusions.—: Multiplex detection of fusions in total nucleic acid purified from FFPE specimens facilitates diagnosis of bone and soft tissue tumors. This technology is particularly useful for morphologically challenging entities and in the absence of prior knowledge of fusion partners, and has the potential to discover novel fusion partners.

Performance Evaluation of a Commercial Real-Time PCR Assay and of an In-House Real-Time PCR for Trypanosoma cruzi DNA Detection in a Tropical Medicine Reference Center, Northern Italy

 

Chagas disease, a neglected protozoal disease endemic in Latin America, is also currently considered an emerging threat in nonendemic areas because of population movements. The detection of Trypanosoma cruzi DNA is increasingly being considered as important evidence to support Chagas disease diagnoses.
However, further performance evaluation of molecular assays is useful for a standardization of strategy considering the whole process in routine diagnosis, especially for the different settings such as endemic and nonendemic countries. Seventy-five samples were collected from subjects screened for Chagas disease in Italy.
The DNA was isolated from blood using automated extraction. We evaluated the performance of the commercial RealCycler® CHAG kit (pmPCR) based on satellite DNA (SatDNA) and of an in-house real-time PCR (ihPCR) targeting Sat and kinetoplast (k) DNAs, using the concordance of two serology assays as a reference standard. The sensitivity of kDNA and SatDNA tests by ihPCR and SatDNA by pmPCR were 14.29% (95% confidence interval (CI) 6.38 to 26.22), 7.14% (95% CI 1.98 to 17.29), and 7.14% (95% CI 1.98 to 17.29), respectively.
Specificity was 100% for all PCR assays and targets. Overall, our results suggest that the preferred approach for clinical laboratories is to combine the kDNA and SatDNA as targets in order to minimize false-negative results increasing sensitivity.

Selection of DNA Aptamers for Subcellular Localization of RBSDV P10 Protein in the Midgut of Small Brown Planthoppers by Emulsion PCR-Based SELEX

Rice black-streaked dwarf virus (RBSDV), classified under the Reoviridae, Fijivirus genus, caused an epidemic in the eastern provinces of China and other East Asian countries and resulted in severe yield loss in rice and wheat production. RBSDV is transmitted by the small brown planthopper (SBPH, Laodelphax striatellus Fallén) in a persistent manner.
In order to provide a stable and cost-effective detection probe, in this study we selected three DNA aptamers (R3, R5 and R11) by an optimized, standardized and time saving emulsion PCR-based SELEX, for the detection of RBSDV outer-shell P10 protein for in situ localization studies in the midgut of SBPH. The specificity of these three DNA aptamers was tested through detection of the P10 protein using an enzyme-linked oligonucleotide assay (ELONA) and aptamer-based dot-blot ELISA.
All three DNA aptamers can be used to detect RBSDV P10 protein by immunofluorescent labeling in the midgut of RBSDV-infected SBPH. These data show that the selected aptamers can be used for the detection of RBSDV P10 protein in vitro and in vivo. This is the first report of aptamers being selected for detection of a rice virus capsid protein.

HCV Seroconversion Panel Donor# 63792 (6 X 1 mL)
HCV6220 6 X 1 mL
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HCV6222 8 X 1 mL
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HCV6223 22 X 1 mL
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HCV6224 6 X 1 mL
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HCV6225 19 X 1 mL
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HCV6226 12 X 1 mL
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HCV6227 7 X 1 mL
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HCV6229 8 X 1 mL
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HCV9041 8 X 1 mL
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HCV9042 6 X 1 mL
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HCV9043 6 X 1 mL
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HCV9044 6 X 1 mL
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HCV9045 8 X 1 mL
EUR 1858.37
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 64254 (5 X 1 mL)
HCV9046 5 X 1 mL
EUR 1858.37
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 64273 (10 X 1 mL)
HCV9047 10 X 1 mL
EUR 1858.37
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 64437 (8 X 1 mL)
HCV9048 8 X 1 mL
EUR 1114.56
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 64766 (5 X 1 mL)
HCV9049 5 X 1 mL
EUR 1114.56
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 66158 (13 X 1 mL)
HCV9050 13 X 1 mL
EUR 1858.37
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 66626 (8 X 1 mL)
HCV9054 8 X 1 mL
EUR 1858.37
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 66732 (11 X 1 mL)
HCV9055 11 X 1 mL
EUR 1858.37
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 66861 (11 X 1 mL)
HCV9057 11 X 1 mL
EUR 1858.37
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 66647 (5 X 1 mL)
HCV9058 5 X 1 mL
EUR 1858.37
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 22708D (5 X 1 mL)
HCV9094 5 X 1 mL
EUR 1858.37
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 1038851 (5 X 1 mL)
HCV9095 5 X 1 mL
EUR 1858.37
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 69089 (10 X 1 mL)
HCV10000 10 X 1 mL
EUR 1183.2
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 69654 (7 X 1 mL)
HCV10002 7 X 1 mL
EUR 1183.2
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 69158 (13 X 1 mL)
HCV10003 13 X 1 mL
EUR 1183.2
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 69028 (6 X 1 mL)
HCV10004 6 X 1 mL
EUR 1183.2
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 67355 (11 X 1 mL)
HCV10007 11 X 1 mL
EUR 1183.2
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 69263 (10 X 1 mL)
HCV10008 10 X 1 mL
EUR 1183.2
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 68896 (6 X 1 mL)
HCV10009 6 X 1 mL
EUR 1183.2
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 65470 (7 X 1 mL)
HCV10010 7 X 1 mL
EUR 1183.2
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 66666 (13 X 1 mL)
HCV10011 13 X 1 mL
EUR 1183.2
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 66747 (13 X 1 mL)
HCV10012 13 X 1 mL
EUR 1183.2
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 67356 (10 X 1 mL)
HCV10013 10 X 1 mL
EUR 1183.2
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 68157 (4 X 1 mL)
HCV10014 4 X 1 mL
EUR 1183.2
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 68628 (12 X 1 mL)
HCV10016 12 X 1 mL
EUR 1183.2
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 65064 (16 X 1 mL)
HCV10017 16 X 1 mL
EUR 1858.37
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 69142 (8 X 1 mL)
HCV10019 8 X 1 mL
EUR 1183.2
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 68744 (13 X 1 mL)
HCV10020 13 X 1 mL
EUR 1183.2
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 68470 (14 X 1 mL)
HCV10021 14 X 1 mL
EUR 1183.2
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 68450 (11 X 1 mL)
HCV10022 11 X 1 mL
EUR 1183.2
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 67505 (21 X 1 mL)
HCV10023 21 X 1 mL
EUR 1183.2
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 66719 (8 X 1 mL)
HCV10024 8 X 1 mL
EUR 1183.2
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 66801 (11 X 1 mL)
HCV10025 11 X 1 mL
EUR 1183.2
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 67391 (12 X 1 mL)
HCV10026 12 X 1 mL
EUR 1858.37
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 66751 (8 X 1 mL)
HCV10027 8 X 1 mL
EUR 1183.2
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 67116 (7 X 1 mL)
HCV10028 7 X 1 mL
EUR 1183.2
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 67539 (16 X 1 mL)
HCV10029 16 X 1 mL
EUR 1183.2
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 65977 (8 X 1 mL)
HCV10030 8 X 1 mL
EUR 1183.2
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 66113 (16 X 1 mL)
HCV10032 16 X 1 mL
EUR 1183.2
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 68629 (10 X 1 mL)
HCV10033 10 X 1 mL
EUR 1183.2
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 68223 (13 X 1 mL)
HCV10036 13 X 1 mL
EUR 1183.2
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 65643 (10 X 1 mL)
HCV10043 10 X 1 mL
EUR 1183.2
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 65982 (4 X 1 mL)
HCV10044 4 X 1 mL
EUR 1183.2
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 66074 (5 X 1 mL)
HCV10045 5 X 1 mL
EUR 1183.2
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 67766 (15 X 1 mL)
HCV10050 15 X 1 mL
EUR 1183.2
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 67787 (14 X 1 mL)
HCV10051 14 X 1 mL
EUR 1183.2
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 67861 (12 X 1 mL)
HCV10052 12 X 1 mL
EUR 1183.2
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 420245049 (7 X 1 mL)
HCV10057 7 X 1 mL
EUR 1858.37
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 420299823 (7 X 1 mL)
HCV10058 7 X 1 mL
EUR 1858.37
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 490104584 (8 X 1 mL)
HCV10062 8 X 1 mL
EUR 1858.37
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 90163052 (7 X 1 mL)
HCV10071 7 X 1 mL
EUR 1858.37
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 72237 (9 X 1 mL)
HCV10165 9 X 1 mL
EUR 1858.37
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 70225 (5 X 1 mL)
HCV10185 5 X 1 mL
EUR 1858.37
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 61025 (5 X 1 mL)
HCV6217 5 X 1 mL
EUR 1114.56
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Pre/Post Seroconversion Panel Donor# 66822 (5 X 1 mL)
HCV10235 5 X 1 mL
EUR 615.36
Description: Please contact Gentaur in order to receive the datasheet of the product.

Positive control tissue section for each antibody; Based on availability INQUIRE
Control-Slides Set of 5
EUR 211.2

Zika Virus Seroconversion Panel (7 X 0.5mL)
KZMC047 7 X 0.5mL
EUR 2840.54
Description: Please contact Gentaur in order to receive the datasheet of the product.

HIV Seroconversion Panel Donor# 65404 (7 X mL)
HIV9013 7 X mL
EUR 1858.37
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Genotype Panel (14 X 0.5 mL)
KZMC049 14 X 0.5 mL
EUR 2629.63
Description: Please contact Gentaur in order to receive the datasheet of the product.

EBV Seroconversion Panel Donor# 283843421 (14 X 1 mL)
EBV9097 14 X 1 mL
EUR 1858.37
Description: Please contact Gentaur in order to receive the datasheet of the product.

HBV Seroconversion Panel Donor# 64090 (5 X 1 mL)
HBV10231 5 X 1 mL
EUR 698.98
Description: Please contact Gentaur in order to receive the datasheet of the product.

HBV Seroconversion Panel Donor# 64017 (6 X 1 mL)
HBV10232 6 X 1 mL
EUR 698.98
Description: Please contact Gentaur in order to receive the datasheet of the product.

HBV Seroconversion Panel Donor# 65449 (9 X 1 mL)
HBV11000 9 X 1 mL
EUR 1030.94
Description: Please contact Gentaur in order to receive the datasheet of the product.

HBV Seroconversion Panel Donor# 65524 (8 X 1 mL)
HBV11001 8 X 1 mL
EUR 1030.94
Description: Please contact Gentaur in order to receive the datasheet of the product.

HBV Seroconversion Panel Donor# 65556 (6 X 1 mL)
HBV11002 6 X 1 mL
EUR 1030.94
Description: Please contact Gentaur in order to receive the datasheet of the product.

HBV Seroconversion Panel Donor# 65584 (8 X 1 mL)
HBV11003 8 X 1 mL
EUR 1030.94
Description: Please contact Gentaur in order to receive the datasheet of the product.

HBV Seroconversion Panel Donor# 65732 (8 X 1 mL)
HBV11004 8 X 1 mL
EUR 1030.94
Description: Please contact Gentaur in order to receive the datasheet of the product.

HBV Seroconversion Panel Donor# 65777 (14 X 1 mL)
HBV11005 14 X 1 mL
EUR 1030.94
Description: Please contact Gentaur in order to receive the datasheet of the product.

HBV Seroconversion Panel Donor# 66201 (17 X 1 mL)
HBV11006 17 X 1 mL
EUR 1030.94
Description: Please contact Gentaur in order to receive the datasheet of the product.

HBV Seroconversion Panel Donor# 66537 (14 X 1 mL)
HBV11007 14 X 1 mL
EUR 1030.94
Description: Please contact Gentaur in order to receive the datasheet of the product.

HBV Seroconversion Panel Donor# 67303 (18 X 1 mL)
HBV11008 18 X 1 mL
EUR 1030.94
Description: Please contact Gentaur in order to receive the datasheet of the product.

HBV Seroconversion Panel Donor# 67449 (23 X 1 mL)
HBV11009 23 X 1 mL
EUR 1030.94
Description: Please contact Gentaur in order to receive the datasheet of the product.

HBV Seroconversion Panel Donor# 67457 (18 X 1 mL)
HBV11010 18 X 1 mL
EUR 1030.94
Description: Please contact Gentaur in order to receive the datasheet of the product.

HBV Seroconversion Panel Donor# 67694 (14 X 1 mL)
HBV11011 14 X 1 mL
EUR 1030.94
Description: Please contact Gentaur in order to receive the datasheet of the product.
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