Determination of the viability of Toxoplasma gondii oocysts by PCR real-time after treatment with propidium monoazide

mhealthinfo

RNase H-dependent PCR enables highly specific amplification of antibody variable domains from single B-cells

 

Immunization-based antibody discovery platforms require robust and effective protocols for the amplification, cloning, expression, and screening of antibodies from large numbers of B-cells in order to effectively capture the diversity of an experienced Ig-repertoire. Multiplex PCR using a series of forward and reverse primers designed to recover antibodies from a range of different germline sequences is challenging because primer design requires the recovery of full length antibody sequences, low starting template concentrations, and the need for all the primers to function under the same PCR conditions.
Here we demonstrate several advantages to incorporating RNase H2-dependent PCR (rh-PCR) into a high-throughput, antibody-discovery platform. Firstly, rh-PCR eliminated primer dimer synthesis to below detectable levels, thereby eliminating clones with a false positive antibody titer. Secondly, by increasing the specificity of PCR, the rh-PCR primers increased the recovery of cognate antibody variable regions from single B-cells, as well as downstream recombinant antibody titers.
mhealthinfo

mhealthinfo

 

Rubella virus RT PCR kit

RTq-H481-50R 50T
EUR 1155.6

Rubella virus PCR kit

PCR-H481-48R 50T
EUR 987.6

Rubella virus PCR kit

PCR-H481-96R 100T
EUR 1335.6

Rubella virus One-Step PCR kit

Oneq-H481-100R 100T
EUR 1932

Rubella virus One-Step PCR kit

Oneq-H481-150R 150T
EUR 2646

Rubella virus One-Step PCR kit

Oneq-H481-50R 50T
EUR 1410

Measles Virus Real Time RT-PCR Kit

GWB-LRB043 25 tests Ask for price

Measles Virus Real Time RT-PCR Kit

GWB-LRB044 25 tests Ask for price

Monkeypox Virus Real Time PCR Kit

PDPS-AR064 1 unit Ask for price
Description: Creative Biogene Monkeypox Virus Real Time PCR Kit is used for the detection of monkeypox Virus in serum or lesion exudate samples by using real time PCR systems. Monkeypox virus (MPV) is a double-stranded DNA, zoonotic virus and a species of the genus Orthopoxvirus in the family Poxviridae. It is one of the human orthopoxviruses that includes variola (VARV), cowpox (CPX), and vaccinia (VACV) viruses. The kit contains a specific ready-to-use system for the detection of the monkeypox Virus. Fluorescence is emitted and measured by the real time systems' optical unit during the PCR.

Monkeypox Virus Real Time PCR Kit

ZD-0076-01 25 tests/kit Ask for price
Description: Monkeypox virus is the virus that causes the disease monkeypox in both humans and animals. Monkeypox virus is an Orthopoxvirus, a genus of the family Poxviridae that contains other viral species that target mammals. The virus is mainly found in tropical rainforest regions of central and West Africa. The primary route of infection is thought to be contact with the infected animals or their bodily fluids. The genome is not segmented and contains a single molecule of linear double-stranded DNA, 185000 nucleotides long. The Monkeypox Virus real time PCR Kit contains a specific ready-to-use system for the detection of the Monkeypox Virusthrough polymerase chain reaction (PCR) in the real-time PCR system. The master contains reagents and enzymes for the specific amplification of the Monkeypox Virus DNA. Fluorescence is emitted and measured by the real time systems ́ optical unit during the PCR. The detection of amplified Monkeypox Virus DNA fragment is performed in fluorimeter channel 530nm with the fluorescent quencher BHQ1. DNA extraction buffer is available in the kit and serum or lesion exudate samples are used for the extraction of the DNA. In addition, the kit contains a system to identify possible PCR inhibition by measuring the 560nm fluorescence of the internal control (IC). An external positive control defined as 1×10^7 copies/ml is supplied which allow the determination of the gene load.

Monkeypox Virus Real Time PCR Kit

ZD-0076-02 25 tests/kit Ask for price
Description: Monkeypox virus is the virus that causes the disease monkeypox in both humans and animals. Monkeypox virus is an Orthopoxvirus, a genus of the family Poxviridae that contains other viral species that target mammals. The virus is mainly found in tropical rainforest regions of central and West Africa. The primary route of infection is thought to be contact with the infected animals or their bodily fluids.The genome is not segmented and contains a single molecule of linear double-stranded DNA, 185000 nucleotides long.The Monkeypox Virus real time PCR Kit contains a specific ready-to-use system for the detection of the Monkeypox Virusthrough polymerase chain reaction (PCR) in the real-time PCR system. The master contains reagents and enzymes for the specific amplification of theMonkeypox VirusDNA. Fluorescence is emitted and measured by the real time systems ́ optical unit during the PCR. The detection of amplified Monkeypox Virus DNA fragment is performed in fluorimeter channelFAM with the fluorescent quencher BHQ1. DNA extraction buffer is available in the kit and serum or lesion exudate samples are used for the extraction of the DNA. In addition, the kit contains a system to identify possible PCR inhibition by measuring the HEX/VIC/JOE fluorescence of the internal control (IC). An external positive control defined as 1×107copies/ml is supplied which allow the determination of the gene load.

Monkeypox Virus Real Time PCR Kit

YJC70115NW-25T 25 tests/kit Ask for price
Description: The Bioperfectus Monkeypox Virus Real Time PCR Kit is an in vitro diagnostic test, based on real-time PCR technology, for the detection of DNA from the Monkeypox virus. Specimens can be obtained from human serum, lesion exudate samples and scab. BSL-2 facilities with standard BSL-2 work practices may be used for the test of t he Monkeypox virus.

Chikungunya Virus Real Time RT-PCR Kit

GWB-LRB001 25 tests Ask for price

Chikungunya Virus Real Time RT-PCR Kit

GWB-LRB002 25 tests Ask for price

Rubella virus

Oneq-H481-OneqH481100R Oneq-H481-100R
EUR 1028

Rubella virus

Oneq-H481-OneqH481150R Oneq-H481-150R
EUR 1438

Rubella virus

Oneq-H481-OneqH48150R Oneq-H481-50R
EUR 728

Rubella virus

PCR-H481-PCRH48148R PCR-H481-48R
EUR 485

Rubella virus

PCR-H481-PCRH48196R PCR-H481-96R
EUR 685

Rubella Virus E2,

GWB-70779B 1 mg Ask for price

Rubella Virus C

GWB-77F49F 1 mg Ask for price

Rubella Virus -HRP

GWB-7ABC27 1 ml Ask for price

Rubella Virus IgG

RUBG0400 96
EUR 98

Rubella Virus Avidity Reagent

B129AVID 1 mL
EUR 85

Rubella VIrus -FITC

GWB-143373 1 ml Ask for price

QPCR Kit RNA Rubella virus

MOL8312 EACH
EUR 788.88

Rubella Virus IgG ELISA Kit

ESR129G 96 Tests
EUR 362

Rubella Virus IgM ELISA Kit

ESR129M 96 Tests
EUR 362

NATIVE RUBELLA VIRUS

GWB-DA7089 1 ml Ask for price

Rubella virus protein

30-1344 1 ml
EUR 497
Description: Purified native Rubella virus protein (Grade III)

Rubella Virus Protein

abx670270-1mg 1 mg
EUR 1212

Rubella Virus Protein

abx670271-1mg 1 mg
EUR 2163.6

Rubella Virus Antigen

PR-BA129G2-L 1mg
EUR 1736.4
Description: native, Vero cell culture

Rubella Virus Antigen

PR-BA129G2-S 250µg
EUR 578.7
Description: native, Vero cell culture

Rubella virus antibody

20-RG04 1 ml
EUR 360
Description: Goat polyclonal Rubella virus antibody

Rubella virus antibody

10R-R119a 1 mg
EUR 444
Description: Mouse monoclonal Antibody raised against Rubella virus

Rubella Virus Antibody

GWB-3BEA8B 1 mg Ask for price

Rubella Virus Antibody

abx023129-1ml 1 ml
EUR 644.4

Rubella virus Antibody

GWB-60F0D7 0.1 mg Ask for price

RUBELLA Virus Antibody

GWB-E871A4 0.2 mg Ask for price

Rubella Virus Antigens

GWB-8DCDE6 1 mg Ask for price

Rubella Virus, Antibody

GWB-D84015 1 mg Ask for price

Rubella Virus Antibody

abx023129-400l 400 µl Ask for price

Rubella Virus Antibody

abx023129-80l 80 µl
EUR 975

Transfusion-transmitted Virus (TTV) Real Time PCR Kit

GWB-LRB041 25 tests Ask for price

Transfusion-transmitted Virus (TTV) Real Time PCR Kit

GWB-LRB042 25 tests Ask for price

Rubella Virus E1 mosaic

GWB-9E07D0 1 mg Ask for price

Rubella Virus E1 Mosaic

PR27125 500 ug
EUR 999.6

Rubella Virus Capsid C

PR27126 500 ug
EUR 999.6

Rubella Virus E1 Mosaic

PR27125-B each
EUR 540

Rubella Virus Capsid C

PR27126-B each
EUR 540

Rubella virus E2 protein

30-1281 1 mg
EUR 1195
Description: Purified recombinant Rubella virus E2 protein

Rubella virus E2 protein

DAGA-498 1mg
EUR 3385.2

Rubella Virus E1 protein

80-1059 1 mg
EUR 1680
Description: Purified recombinant Rubella Virus E1 protein

Rubella Virus E2 Protein

abx060648-1mg 1 mg
EUR 2055.6

Rubella Virus E2 Protein

abx060649-1mg 1 mg
EUR 1713.6

Rubella Virus E2 Protein

20-abx260148
  • Ask for price
  • Ask for price
  • Ask for price
  • 0.5 mg
  • 100 ug
  • 1 mg

Rubella Virus E2 Protein

abx260148-20g 20 µg
EUR 950

Rubella Virus E2 Protein

abx260148-5g 5 µg
EUR 350

Rubella Virus E1 Antibody

abx022204-1mg 1 mg
EUR 718.8

Rubella Virus E1 Antibody

abx022205-1mg 1 mg
EUR 610.8

Rubella Virus E1 Antibody

abx022206-1mg 1 mg
EUR 610.8

Rubella Virus E1 Antibody

abx022206-400l 400 µl Ask for price

Rubella Virus E1 Antibody

abx022206-80l 80 µl
EUR 550

Rubella virus antibody (HRP)

60-RG05 1 ml
EUR 405
Description: Goat polyclonal Rubella virus antibody (HRP) conjugated

Rubella Virus Antibody (HRP)

abx023126-1ml 1 ml
EUR 718.8

Rubella Virus Antibody (HRP)

abx023126-400l 400 µl Ask for price

Rubella Virus Antibody (HRP)

abx023126-80l 80 µl
EUR 1112.5

Rubella virus antibody (FITC)

60-RG04 1 ml
EUR 385
Description: Goat polyclonal Rubella virus antibody (FITC) conjugated

Rubella Virus Antibody (FITC)

abx023127-1ml 1 ml
EUR 661.2

Rubella Virus Antibody (FITC)

abx023127-400l 400 µl Ask for price

Rubella Virus Antibody (FITC)

abx023127-80l 80 µl
EUR 1112.5

Rubella Virus E2 (Recombinant)

22060684-1 100 µg
EUR 264.47

Rubella Virus IgG Immunoblot

GWB-521231 1x96 Assays Ask for price

Rubella Virus (German Measles)

GWB-1B7BFC 1 ml Ask for price

Recombinant Rubella Virus E2

7-07861 100µg Ask for price

Recombinant Rubella Virus E2

7-07862 500µg Ask for price

Recombinant Rubella Virus E2

7-07863 1000µg Ask for price

Rubella Virus Capsid Protein

abx060645-1mg 1 mg
EUR 2180.4

Rubella Virus E2 Recombinant

rAP-5458 Inquiry Ask for price

Rubella Virus Virion Antibody

GWB-2DDE26 1 ml Ask for price

Rubella Virus Virions Antibody

GWB-CDDDE8 1 ml Ask for price

Rubella Virus IgG quantitative

GWB-31CB3E 1x96 Assays Ask for price

Rubella virus-like particles

REC32047-100 0.1
EUR 504.63
Description:
Rubella virus-like particles produced in a HEK293 stable cell line. Particles are secreted into the culture supernatant from stably transfected cells and purified by isopycnic centrifugation.

Rubella virus-like particles

REC32047-500 0.5
EUR 2144.68
Description:
Rubella virus-like particles produced in a HEK293 stable cell line. Particles are secreted into the culture supernatant from stably transfected cells and purified by isopycnic centrifugation.

Recombinant Rubella Virus Antigen

DAG-WT1047 1 mg
EUR 495
Description: Recombinant

Rubella Virus E1 protein [GST]

DAGA-499 1mg
EUR 4446

Rubella Virus IgM µ-capture

RUBM0400 96
EUR 104

Rubella Virus (strain HPV-77)

DAGA-2019 1 mg
EUR 1420.65
Description: Native

Recombinant Rubella Virus Rubella E1 Protein, GST, E.coli-1mg

QP13373-1mg 1mg
EUR 1513.2

Rat Rubella virus antibody IgG ELISA Kit

ERR0019 96Tests
EUR 625.2

Rat Rubella virus antibody IgA ELISA Kit

ERR0020 96Tests
EUR 625.2

Rat Rubella virus antibody IgM ELISA Kit

ERR0021 96Tests
EUR 625.2

Rubella virus protein (Grade 1)

30-1330 1 ml
EUR 786
Description: Purified Native Rubella virus protein (Grade 1)

Rubella virus protein (Grade 2)

30-1331 1 mg
EUR 2018.4
Description: Purified Native Rubella virus protein (Grade 2)

Rubella Virus Grade 2 Antigen

BA129G2VS 1mg
EUR 1082

Recombinant Rubella Virus Rubella E1 Protein, GST, E.coli-100ug

QP13373-100ug 100ug
EUR 261.6

Recombinant Rubella Virus Rubella E1 Protein, GST, E.coli-500ug

QP13373-500ug 500ug
EUR 795.6

Rubella Virus IgM -capture ELISA

GWB-1D2E67 1x96 Assays Ask for price

Rubella Virus Capsid C protein

80-1058 100 ug
EUR 210
Description: Purified recombinant Rubella Virus Capsid C protein

Rubella Virus E1 Mosaic Protein

abx060646-1mg 1 mg
EUR 2214

Rubella Virus E1 Mosaic Protein

abx060647-1mg 1 mg
EUR 1796.4

Rubella Virus Capsid C Protein

20-abx260149
  • Ask for price
  • Ask for price
  • Ask for price
  • 0.5 mg
  • 100 ug
  • 1 mg

Rubella Virus E1 Mosaic Protein

20-abx260310
  • Ask for price
  • Ask for price
  • Ask for price
  • 0.5 mg
  • 100 ug
  • 1 mg

Rubella Virus IgG Control Serum

C129G 3 mL
EUR 105

Rubella Virus IgM Control Serum

C129M 3 mL
EUR 105

Rubella Virus E1 Mosaic Protein

abx260310-20g 20 µg
EUR 950

Rubella Virus E1 Mosaic Protein

abx260310-5g 5 µg
EUR 350

Rubella Virus Capsid C Protein

abx260149-25g 25 µg
EUR 950

Rubella Virus Capsid C Protein

abx260149-5g 5 µg
EUR 350

Mouse Rubella virus antibody IgG ELISA Kit

EMR0019 96Tests
EUR 625.2

Mouse Rubella virus antibody IgA ELISA Kit

EMR0020 96Tests
EUR 625.2

Mouse Rubella virus antibody IgM ELISA Kit

EMR0021 96Tests
EUR 625.2

Human Rubella virus antibody IgG ELISA Kit

EHR0019 96Tests
EUR 625.2

Human Rubella virus antibody IgA ELISA Kit

EHR0020 96Tests
EUR 625.2

Human Rubella virus antibody IgM ELISA Kit

EHR0021 96Tests
EUR 625.2

Human Rubella Virus IgM Antibody ELISA Kit

abx364857-96tests 96 tests
EUR 548.4

Human Rubella Virus IgG Antibody ELISA Kit

abx364858-96tests 96 tests
EUR 548.4

Human Rubella Virus IgG Antibody ELISA Kit

abx053898-96tests 96 tests
EUR 801.6

Human Rubella Virus IgM Antibody ELISA Kit

abx054089-96tests 96 tests
EUR 801.6

Bovine Rubella Virus Antibody IgG ELSA KIT

QY-E60087 96T
EUR 511.2

Recombinant Rubella Virus Rubella Capsid Protein, GST, E.coli-1mg

QP13372-1mg 1mg
EUR 1273.2

Human Rubella Virus IgG (RV IgG) ELISA Kit

abx053898-100g 100 µg Ask for price

Human Rubella Virus IgG (RV IgG) ELISA Kit

abx053898-10g 10 µg
EUR 493.75

Human Rubella Virus IgG (RV IgG) ELISA Kit

abx053898-50g 50 µg Ask for price

Human Rubella Virus IgM (RV IgM) ELISA Kit

abx054089-100g 100 µg Ask for price

Human Rubella Virus IgM (RV IgM) ELISA Kit

abx054089-10g 10 µg
EUR 493.75

Human Rubella Virus IgM (RV IgM) ELISA Kit

abx054089-50g 50 µg Ask for price

Recombinant Rubella Virus Rubella E2 Protein, Untagged, E.coli-1mg

QP13374-1mg 1mg
EUR 1273.2

Recombinant Rubella Virus Rubella Capsid Protein, GST, E.coli-100ug

QP13372-100ug 100ug
EUR 261.6

Recombinant Rubella Virus Rubella Capsid Protein, GST, E.coli-500ug

QP13372-500ug 500ug
EUR 795.6

Human Rubella Virus (RV) IgG Rapid Test Kit

abx092097-20tests 20 tests
EUR 260.4

Recombinant Rubella Virus Rubella E2 Protein, Untagged, E.coli-100ug

QP13374-100ug 100ug
EUR 261.6

Recombinant Rubella Virus Rubella E2 Protein, Untagged, E.coli-500ug

QP13374-500ug 500ug
EUR 795.6

Rubella Virus Capsid C (Recombinant)

22060682-1 100 µg
EUR 237.01

Rubella Virus E1 Mosaic (Recombinant)

22060683-1 100 µg
EUR 264.47

Recombinant Rubella Virus E1 Mosaic

7-07858 100µg Ask for price

Recombinant Rubella Virus E1 Mosaic

7-07859 500µg Ask for price

Recombinant Rubella Virus E1 Mosaic

7-07860 1000µg Ask for price

Recombinant Rubella Virus Capsid C

7-07864 100µg Ask for price

Recombinant Rubella Virus Capsid C

7-07865 500µg Ask for price

Recombinant Rubella Virus Capsid C

7-07866 1000µg Ask for price

Rubella Virus Core Protein Antibody

GWB-T00630 1 mg Ask for price

Rubella virus core antigen Antibody

GWB-86609B 0.1 mg Ask for price

Rubella Virus E1 Mosaic Recombinant

rAP-5457 Inquiry Ask for price

Rubella Virus Capsid C Recombinant

rAP-5459 Inquiry Ask for price

Rubella virus E2 recombinant antigen

00216-V-01mg 0,1 mg
EUR 321
Description: Rubella virus E2 recombinant antigen a.a. 31-105.

Rubella virus E2 recombinant antigen

00216-V-1000ug 1000 ug
EUR 1539
Description: Rubella virus E2 recombinant antigen a.a. 31-105.

Recombinant Rubella Virus E2 Protein

DAG514 1mg
EUR 1537.2

Rubella virus E2 Recombinant Protein

53-354 0.01 mg
EUR 714.3
Description: Rubella virus E2 Recombinant Protein

Rubella virus E1 Recombinant Protein

53-355 0.01 mg
EUR 714.3
Description: Rubella virus E1 Recombinant Protein

Rubella virus E1 recombinant antigen

8806 100 ug
EUR 529.8
Description: This is Rubella virus E1 recombinant antigen for ELISA,WB.

Rubella virus E2 recombinant antigen

8807 100 ug
EUR 529.8
Description: This is Rubella virus E2 recombinant antigen for ELISA,WB.

Rubella Virus E2, Recombinant Protein

GWB-T00055 1mg Ask for price

Rubella Virus Capsid Protein Antibody

abx022208-1mg 1 mg
EUR 610.8

Rubella Virus Capsid Protein Antibody

GWB-751C39 0.2 mg Ask for price

OPEF01327-1MG - Rubella Virus Antigen

OPEF01327-1MG 1mg
EUR 1589

Rubella Virus Capsid Protein Antibody

abx022208-400l 400 µl Ask for price
Finally, we demonstrate that rh-PCR primers provide a more homogeneous sample pool and greater sequence quality in a Next Generation Sequencing-based approach to obtaining DNA sequence information from large numbers of cloned antibody cognate pairs. Furthermore, the higher specificity of the rh-PCR primers allowed for a better match between native antibody germline sequences and the VL/VH fragments amplified from single B-cells.

Determination of the viability of Toxoplasma gondii oocysts by PCR real-time after treatment with propidium monoazide

 

This study aimed to investigate a methodology for discriminating viable and non-viable T. gondii oocysts in water. Analyses included two steps: (i) microscopic investigation with vital dyes; (ii) molecular investigation, using a real time PCR (qPCR), after parasite treatment (or not) with propidium monoazide (PMA).
The method was called qPCR-PMA. Oocyst aliquots were incubated (15 min) at 25 ºC or 100 ºC and analyzed by microscopy, after trypan blue and neutral red staining. Microscopic investigation determined viable and non-viable oocysts. For the molecular investigation, both aliquots of oocysts were treated with PMA. Non-viable oocysts, after PMA treatment, exhibited an inhibition of DNA amplification by qPCR. Although analyses were carried out with oocysts treated experimentally, these results suggest that qPCR-PMA can be a useful strategy to distinguish viable and non-viable T. gondii oocysts in water safety testing, showing if water is safe to drink.

Diagnostic Utility of a Custom 34-Gene Anchored Multiplex PCR-Based Next-Generation Sequencing Fusion Panel for the Diagnosis of Bone and Soft Tissue Neoplasms With Identification of Novel USP6 Fusion Partners in Aneurysmal Bone Cysts

 

Context.—: Bone and soft tissue tumors are heterogeneous, diagnostically challenging, and often defined by gene fusions.
Objective.—: To present our experience using a custom 34-gene targeted sequencing fusion panel.
Design.—: Total nucleic acid extracted from formalin-fixed, paraffin-embedded (FFPE) tumor specimens was subjected to open-ended, nested anchored multiplex polymerase chain reaction and enrichment of 34 gene targets, thus enabling detection of known and novel fusion partners.
Results.—: During a 12-month period, 145 patients were tested as part of routine clinical care. Tumor percentage ranged from 10% to 100% and turnaround time ranged from 3 to 15 (median, 7.9) days. The most common diagnostic groups were small round blue cell tumors, tumors of uncertain differentiation, fibroblastic/myofibroblastic tumors, and adipocytic tumors.
In-frame fusion transcripts were identified in 64 of 140 cases sequenced (46%): in 62 cases, the detection of a disease-defining fusion confirmed the morphologic impression; in 2 cases, a germline TFG-GPR128 polymorphic fusion variant was detected. Several genes in the panel partnered with multiple fusion partners specific for different diagnoses, for example, EWSR1, NR4A3, FUS, NCOA2, and TFE3. Interesting examples are presented to highlight how fusion detection or lack thereof was instrumental in establishing accurate diagnoses. Novel fusion partners were detected for 2 cases of solid aneurysmal bone cysts (PTBP1-USP6, SLC38A2-USP6).
Conclusions.—: Multiplex detection of fusions in total nucleic acid purified from FFPE specimens facilitates diagnosis of bone and soft tissue tumors. This technology is particularly useful for morphologically challenging entities and in the absence of prior knowledge of fusion partners, and has the potential to discover novel fusion partners.

Performance Evaluation of a Commercial Real-Time PCR Assay and of an In-House Real-Time PCR for Trypanosoma cruzi DNA Detection in a Tropical Medicine Reference Center, Northern Italy

 

Chagas disease, a neglected protozoal disease endemic in Latin America, is also currently considered an emerging threat in nonendemic areas because of population movements. The detection of Trypanosoma cruzi DNA is increasingly being considered as important evidence to support Chagas disease diagnoses.
However, further performance evaluation of molecular assays is useful for a standardization of strategy considering the whole process in routine diagnosis, especially for the different settings such as endemic and nonendemic countries. Seventy-five samples were collected from subjects screened for Chagas disease in Italy.
The DNA was isolated from blood using automated extraction. We evaluated the performance of the commercial RealCycler® CHAG kit (pmPCR) based on satellite DNA (SatDNA) and of an in-house real-time PCR (ihPCR) targeting Sat and kinetoplast (k) DNAs, using the concordance of two serology assays as a reference standard. The sensitivity of kDNA and SatDNA tests by ihPCR and SatDNA by pmPCR were 14.29% (95% confidence interval (CI) 6.38 to 26.22), 7.14% (95% CI 1.98 to 17.29), and 7.14% (95% CI 1.98 to 17.29), respectively.
Specificity was 100% for all PCR assays and targets. Overall, our results suggest that the preferred approach for clinical laboratories is to combine the kDNA and SatDNA as targets in order to minimize false-negative results increasing sensitivity.

Selection of DNA Aptamers for Subcellular Localization of RBSDV P10 Protein in the Midgut of Small Brown Planthoppers by Emulsion PCR-Based SELEX

Rice black-streaked dwarf virus (RBSDV), classified under the Reoviridae, Fijivirus genus, caused an epidemic in the eastern provinces of China and other East Asian countries and resulted in severe yield loss in rice and wheat production. RBSDV is transmitted by the small brown planthopper (SBPHLaodelphax striatellus Fallén) in a persistent manner.
In order to provide a stable and cost-effective detection probe, in this study we selected three DNA aptamers (R3, R5 and R11) by an optimized, standardized and time saving emulsion PCR-based SELEX, for the detection of RBSDV outer-shell P10 protein for in situ localization studies in the midgut of SBPH. The specificity of these three DNA aptamers was tested through detection of the P10 protein using an enzyme-linked oligonucleotide assay (ELONA) and aptamer-based dot-blot ELISA.
All three DNA aptamers can be used to detect RBSDV P10 protein by immunofluorescent labeling in the midgut of RBSDV-infected SBPH. These data show that the selected aptamers can be used for the detection of RBSDV P10 protein in vitro and in vivo. This is the first report of aptamers being selected for detection of a rice virus capsid protein.

HCV Seroconversion Panel Donor# 69158 (13 X 1 mL)

HCV10003 13 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 69028 (6 X 1 mL)

HCV10004 6 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 67355 (11 X 1 mL)

HCV10007 11 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 69263 (10 X 1 mL)

HCV10008 10 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 68896 (6 X 1 mL)

HCV10009 6 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 65470 (7 X 1 mL)

HCV10010 7 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 66666 (13 X 1 mL)

HCV10011 13 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 66747 (13 X 1 mL)

HCV10012 13 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 67356 (10 X 1 mL)

HCV10013 10 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 68157 (4 X 1 mL)

HCV10014 4 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 68628 (12 X 1 mL)

HCV10016 12 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 65064 (16 X 1 mL)

HCV10017 16 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 69142 (8 X 1 mL)

HCV10019 8 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 68744 (13 X 1 mL)

HCV10020 13 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 68470 (14 X 1 mL)

HCV10021 14 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 68450 (11 X 1 mL)

HCV10022 11 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 67505 (21 X 1 mL)

HCV10023 21 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 66719 (8 X 1 mL)

HCV10024 8 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 66801 (11 X 1 mL)

HCV10025 11 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 67391 (12 X 1 mL)

HCV10026 12 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 66751 (8 X 1 mL)

HCV10027 8 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 67116 (7 X 1 mL)

HCV10028 7 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 67539 (14 X 1 mL)

HCV10029 16 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 65977 (8 X 1 mL)

HCV10030 8 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 66113 (16 X 1 mL)

HCV10032 16 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 68629 (10 X 1 mL)

HCV10033 10 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 68223 (13 X 1 mL)

HCV10036 13 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 65643 (10 X 1 mL)

HCV10043 10 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 65982 (4 X 1 mL)

HCV10044 4 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 66074 (5 X 1 mL)

HCV10045 5 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 67766 (15 X 1 mL)

HCV10050 15 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 67787 (14 X 1 mL)

HCV10051 14 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 67861 (12 X 1 mL)

HCV10052 12 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 420245049 (7 X 1 mL)

HCV10057 7 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 420299823 (7 X 1 mL)

HCV10058 7 X 1 mL
EUR 1858.37
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 490104584 (8 X 1 mL)

HCV10062 8 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 90163052 (7 X 1 mL)

HCV10071 7 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 72237 (9 X 1 mL)

HCV10165 9 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 70225 (5 X 1 mL)

HCV10185 5 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 61025 (5 X 1 mL)

HCV6217 5 X 1 mL
EUR 1035

HCV Seroconversion Panel Donor# 61251 (8 X 1 mL)

HCV6218 8 X 1 mL
EUR 1035

HCV Seroconversion Panel Donor# 60749 (14 X 1 mL)

HCV6219 14 X 1 mL
EUR 1035

HCV Seroconversion Panel Donor# 63792 (6 X 1 mL)

HCV6220 6 X 1 mL
EUR 1035

HCV Seroconversion Panel Donor# 62258 (8 X 1 mL)

HCV6221 8 X 1 mL
EUR 1035

HCV Seroconversion Panel Donor# 62286 (8 X 1 mL)

HCV6222 8 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 62507 (22 X 1 mL)

HCV6223 22 X 1 mL
EUR 1035

HCV Seroconversion Panel Donor# 62680 (6 X 1 mL)

HCV6224 6 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 62999 (19 X 1 mL)

HCV6225 19 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 63135 (12 X 1 mL)

HCV6226 12 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 63318 (7 X 1 mL)

HCV6227 7 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 62886 (12 X 1 mL)

HCV6228 12 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 62804 (8 X 1 mL)

HCV6229 8 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 63625 (8 X 1 mL)

HCV9041 8 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 63792 (6 X 1 mL)

HCV9042 6 X 1 mL
EUR 1035

HCV Seroconversion Panel Donor# 63869 (6 X 1 mL)

HCV9043 6 X 1 mL
EUR 1035

HCV Seroconversion Panel Donor# 63911 (6 X 1 mL)

HCV9044 6 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 64150 (8 X 1 mL)

HCV9045 8 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 64254 (5 X 1 mL)

HCV9046 5 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 64273 (10 X 1 mL)

HCV9047 10 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 64437 (8 X 1 mL)

HCV9048 8 X 1 mL
EUR 1035

HCV Seroconversion Panel Donor# 64766 (5 X 1 mL)

HCV9049 5 X 1 mL
EUR 1035

HCV Seroconversion Panel Donor# 66158 (13 X 1 mL)

HCV9050 13 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 66626 (8 X 1 mL)

HCV9054 8 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 66732 (11 X 1 mL)

HCV9055 11 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 66861 (11 X 1 mL)

HCV9057 11 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 66647 (5 X 1 mL)

HCV9058 5 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 1038851 (5 X 1 mL)

HCV9095 5 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 22708D (5 X 1 mL)

HCV9094 5 X 1 mL
EUR 1764

HCV Pre/Post Seroconversion Panel Donor# 66822 (5 X 1 mL)

HCV10235 5 X 1 mL
EUR 545

Zika Virus Seroconversion Panel (7 X 0.5mL)

KZMC047 7 X 0.5mL
EUR 2890

HIV Seroconversion Panel Donor# 65404 (7 X mL)

HIV9013 7 X mL
EUR 1764

EBV Seroconversion Panel Donor# 283843421 (14 X 1 mL)

EBV9097 14 X 1 mL
EUR 1764

HBV Seroconversion Panel Donor# 64090 (5 X 1 mL)

HBV10231 5 X 1 mL
EUR 627

HBV Seroconversion Panel Donor# 64017 (6 X 1 mL)

HBV10232 6 X 1 mL
EUR 627

HBV Seroconversion Panel Donor# 65449 (9 X 1 mL)

HBV11000 9 X 1 mL
EUR 953

HBV Seroconversion Panel Donor# 65524 (8 X 1 mL)

HBV11001 8 X 1 mL
EUR 953

HBV Seroconversion Panel Donor# 65556 (6 X 1 mL)

HBV11002 6 X 1 mL
EUR 953

HBV Seroconversion Panel Donor# 65584 (8 X 1 mL)

HBV11003 8 X 1 mL
EUR 953

HBV Seroconversion Panel Donor# 65732 (8 X 1 mL)

HBV11004 8 X 1 mL
EUR 953

HBV Seroconversion Panel Donor# 65777 (14 X 1 mL)

HBV11005 14 X 1 mL
EUR 953

HBV Seroconversion Panel Donor# 66201 (17 X 1 mL)

HBV11006 17 X 1 mL
EUR 953

HBV Seroconversion Panel Donor# 66537 (14 X 1 mL)

HBV11007 14 X 1 mL
EUR 953

HBV Seroconversion Panel Donor# 67303 (18 X 1 mL)

HBV11008 18 X 1 mL
EUR 953

HBV Seroconversion Panel Donor# 67449 (23 X 1 mL)

HBV11009 23 X 1 mL
EUR 953

HBV Seroconversion Panel Donor# 67457 (18 X 1 mL)

HBV11010 18 X 1 mL
EUR 953

HBV Seroconversion Panel Donor# 67694 (14 X 1 mL)

HBV11011 14 X 1 mL
EUR 953

HBV Seroconversion Panel Donor# 67773 (6 X 1 mL)

HBV11012 6 X 1 mL
EUR 953

HBV Seroconversion Panel Donor# 67962 (35 X 1 mL)

HBV11013 35 X 1 mL
EUR 953