Determination of the viability of Toxoplasma gondii oocysts by PCR real-time after treatment with propidium monoazide

November 12, 2020
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RNase H-dependent PCR enables highly specific amplification of antibody variable domains from single B-cells

 

Immunization-based antibody discovery platforms require robust and effective protocols for the amplification, cloning, expression, and screening of antibodies from large numbers of B-cells in order to effectively capture the diversity of an experienced Ig-repertoire. Multiplex PCR using a series of forward and reverse primers designed to recover antibodies from a range of different germline sequences is challenging because primer design requires the recovery of full length antibody sequences, low starting template concentrations, and the need for all the primers to function under the same PCR conditions.
Here we demonstrate several advantages to incorporating RNase H2-dependent PCR (rh-PCR) into a high-throughput, antibody-discovery platform. Firstly, rh-PCR eliminated primer dimer synthesis to below detectable levels, thereby eliminating clones with a false positive antibody titer. Secondly, by increasing the specificity of PCR, the rh-PCR primers increased the recovery of cognate antibody variable regions from single B-cells, as well as downstream recombinant antibody titers.
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Monkeypox Virus Real Time PCR Kit
PDPS-AR064 1 unit Ask for price
Description: Creative Biogene Monkeypox Virus Real Time PCR Kit is used for the detection of monkeypox Virus in serum or lesion exudate samples by using real time PCR systems. Monkeypox virus (MPV) is a double-stranded DNA, zoonotic virus and a species of the genus Orthopoxvirus in the family Poxviridae. It is one of the human orthopoxviruses that includes variola (VARV), cowpox (CPX), and vaccinia (VACV) viruses. The kit contains a specific ready-to-use system for the detection of the monkeypox Virus. Fluorescence is emitted and measured by the real time systems' optical unit during the PCR.

Monkeypox Virus Real Time PCR Kit
ZD-0076-01 25 tests/kit Ask for price
Description: Monkeypox virus is the virus that causes the disease monkeypox in both humans and animals. Monkeypox virus is an Orthopoxvirus, a genus of the family Poxviridae that contains other viral species that target mammals. The virus is mainly found in tropical rainforest regions of central and West Africa. The primary route of infection is thought to be contact with the infected animals or their bodily fluids. The genome is not segmented and contains a single molecule of linear double-stranded DNA, 185000 nucleotides long. The Monkeypox Virus real time PCR Kit contains a specific ready-to-use system for the detection of the Monkeypox Virusthrough polymerase chain reaction (PCR) in the real-time PCR system. The master contains reagents and enzymes for the specific amplification of the Monkeypox Virus DNA. Fluorescence is emitted and measured by the real time systems ́ optical unit during the PCR. The detection of amplified Monkeypox Virus DNA fragment is performed in fluorimeter channel 530nm with the fluorescent quencher BHQ1. DNA extraction buffer is available in the kit and serum or lesion exudate samples are used for the extraction of the DNA. In addition, the kit contains a system to identify possible PCR inhibition by measuring the 560nm fluorescence of the internal control (IC). An external positive control defined as 1×10^7 copies/ml is supplied which allow the determination of the gene load.

Monkeypox Virus Real Time PCR Kit
ZD-0076-02 25 tests/kit Ask for price
Description: Monkeypox virus is the virus that causes the disease monkeypox in both humans and animals. Monkeypox virus is an Orthopoxvirus, a genus of the family Poxviridae that contains other viral species that target mammals. The virus is mainly found in tropical rainforest regions of central and West Africa. The primary route of infection is thought to be contact with the infected animals or their bodily fluids.The genome is not segmented and contains a single molecule of linear double-stranded DNA, 185000 nucleotides long.The Monkeypox Virus real time PCR Kit contains a specific ready-to-use system for the detection of the Monkeypox Virusthrough polymerase chain reaction (PCR) in the real-time PCR system. The master contains reagents and enzymes for the specific amplification of theMonkeypox VirusDNA. Fluorescence is emitted and measured by the real time systems ́ optical unit during the PCR. The detection of amplified Monkeypox Virus DNA fragment is performed in fluorimeter channelFAM with the fluorescent quencher BHQ1. DNA extraction buffer is available in the kit and serum or lesion exudate samples are used for the extraction of the DNA. In addition, the kit contains a system to identify possible PCR inhibition by measuring the HEX/VIC/JOE fluorescence of the internal control (IC). An external positive control defined as 1×107copies/ml is supplied which allow the determination of the gene load.

Monkeypox Virus Real Time PCR Kit
YJC70115NW-25T 25 tests/kit Ask for price
Description: The Bioperfectus Monkeypox Virus Real Time PCR Kit is an in vitro diagnostic test, based on real-time PCR technology, for the detection of DNA from the Monkeypox virus. Specimens can be obtained from human serum, lesion exudate samples and scab. BSL-2 facilities with standard BSL-2 work practices may be used for the test of t he Monkeypox virus.

QPCR Kit RNA Rubella virus
MOL8312 EACH
EUR 788.88

Rubella virus protein
30-1344 1 ml
EUR 457.2
Description: Purified native Rubella virus protein (Grade III)

Rubella Virus Protein
abx670270-1mg 1 mg
EUR 1212

Rubella Virus Protein
abx670271-1mg 1 mg
EUR 2163.6

Rubella virus antibody
10R-R119a 1 mg
EUR 444
Description: Mouse monoclonal Antibody raised against Rubella virus

Rubella virus antibody
20-RG04 1 ml
EUR 440.4
Description: Goat polyclonal Rubella virus antibody

Rubella Virus Antibody
abx023129-1ml 1 ml
EUR 644.4

Rubella Virus E1 Mosaic
PR27125 500 ug
EUR 999.6

Rubella Virus Capsid C
PR27126 500 ug
EUR 999.6

Rubella Virus E2 Protein
20-abx260148
  • EUR 1062.00
  • EUR 410.40
  • EUR 1646.40
  • 0.5 mg
  • 100 ug
  • 1 mg

Rubella virus E2 protein
30-1281 1 mg
EUR 1585.2
Description: Purified recombinant Rubella virus E2 protein

Rubella Virus E1 protein
80-1059 1 mg
EUR 2058
Description: Purified recombinant Rubella Virus E1 protein

Rubella Virus E2 Protein
abx060648-1mg 1 mg
EUR 2055.6

Rubella Virus E2 Protein
abx060649-1mg 1 mg
EUR 1713.6

Rubella virus E2 protein
DAGA-498 1mg
EUR 3385.2

Rubella Virus E1 Antibody
abx022204-1mg 1 mg
EUR 718.8

Rubella Virus E1 Antibody
abx022205-1mg 1 mg
EUR 610.8

Rubella Virus E1 Antibody
abx022206-1mg 1 mg
EUR 610.8

Rubella Virus Antibody (HRP)
abx023126-1ml 1 ml
EUR 718.8

Rubella virus antibody (HRP)
60-RG05 1 ml
EUR 480
Description: Goat polyclonal Rubella virus antibody (HRP) conjugated

Rubella Virus Antibody (FITC)
abx023127-1ml 1 ml
EUR 661.2

Rubella virus antibody (FITC)
60-RG04 1 ml
EUR 440.4
Description: Goat polyclonal Rubella virus antibody (FITC) conjugated

Rubella Virus Capsid Protein
abx060645-1mg 1 mg
EUR 2180.4

Recombinant Rubella Virus E2
7-07861 100µg Ask for price

Recombinant Rubella Virus E2
7-07862 500µg Ask for price

Recombinant Rubella Virus E2
7-07863 1000µg Ask for price

Rubella Virus E1 protein [GST]
DAGA-499 1mg
EUR 4446

Rubella virus protein (Grade 1)
30-1330 1 ml
EUR 975.6
Description: Purified Native Rubella virus protein (Grade 1)

Rubella virus protein (Grade 2)
30-1331 1 mg
EUR 2018.4
Description: Purified Native Rubella virus protein (Grade 2)

Rubella Virus Capsid C Protein
20-abx260149
  • EUR 1062.00
  • EUR 410.40
  • EUR 1646.40
  • 0.5 mg
  • 100 ug
  • 1 mg

Rubella Virus E1 Mosaic Protein
20-abx260310
  • EUR 1062.00
  • EUR 410.40
  • EUR 1930.80
  • 0.5 mg
  • 100 ug
  • 1 mg

Rubella Virus Capsid C protein
80-1058 100 ug
EUR 548.4
Description: Purified recombinant Rubella Virus Capsid C protein

Rubella Virus E1 Mosaic Protein
abx060646-1mg 1 mg
EUR 2214

Rubella Virus E1 Mosaic Protein
abx060647-1mg 1 mg
EUR 1796.4

Recombinant Rubella Virus Rubella E1 Protein, GST, E.coli-1mg
QP13373-1mg 1mg
EUR 1513.2

Recombinant Rubella Virus Rubella E1 Protein, GST, E.coli-100ug
QP13373-100ug 100ug
EUR 261.6

Recombinant Rubella Virus Rubella E1 Protein, GST, E.coli-500ug
QP13373-500ug 500ug
EUR 795.6

Rat Rubella virus antibody IgG ELISA Kit
ERR0019 96Tests
EUR 625.2

Rat Rubella virus antibody IgA ELISA Kit
ERR0020 96Tests
EUR 625.2

Rat Rubella virus antibody IgM ELISA Kit
ERR0021 96Tests
EUR 625.2

Recombinant Rubella Virus Rubella Capsid Protein, GST, E.coli-1mg
QP13372-1mg 1mg
EUR 1273.2

Recombinant Rubella Virus Rubella E2 Protein, Untagged, E.coli-1mg
QP13374-1mg 1mg
EUR 1273.2

Human Rubella Virus IgM Antibody ELISA Kit
abx364857-96tests 96 tests
EUR 548.4

Human Rubella Virus IgG Antibody ELISA Kit
abx364858-96tests 96 tests
EUR 548.4

Human Rubella Virus IgG Antibody ELISA Kit
abx053898-96tests 96 tests
EUR 801.6

Human Rubella Virus IgM Antibody ELISA Kit
abx054089-96tests 96 tests
EUR 801.6

Mouse Rubella virus antibody IgG ELISA Kit
EMR0019 96Tests
EUR 625.2

Mouse Rubella virus antibody IgA ELISA Kit
EMR0020 96Tests
EUR 625.2

Mouse Rubella virus antibody IgM ELISA Kit
EMR0021 96Tests
EUR 625.2

Human Rubella virus antibody IgG ELISA Kit
EHR0019 96Tests
EUR 625.2

Human Rubella virus antibody IgA ELISA Kit
EHR0020 96Tests
EUR 625.2

Human Rubella virus antibody IgM ELISA Kit
EHR0021 96Tests
EUR 625.2

Bovine Rubella Virus Antibody IgG ELSA KIT
QY-E60087 96T
EUR 511.2

Recombinant Rubella Virus E1 Mosaic
7-07858 100µg Ask for price

Recombinant Rubella Virus E1 Mosaic
7-07859 500µg Ask for price

Recombinant Rubella Virus E1 Mosaic
7-07860 1000µg Ask for price

Recombinant Rubella Virus Capsid C
7-07864 100µg Ask for price

Recombinant Rubella Virus Capsid C
7-07865 500µg Ask for price

Recombinant Rubella Virus Capsid C
7-07866 1000µg Ask for price

Recombinant Rubella Virus Rubella Capsid Protein, GST, E.coli-100ug
QP13372-100ug 100ug
EUR 261.6

Recombinant Rubella Virus Rubella Capsid Protein, GST, E.coli-500ug
QP13372-500ug 500ug
EUR 795.6

Recombinant Rubella Virus Rubella E2 Protein, Untagged, E.coli-100ug
QP13374-100ug 100ug
EUR 261.6

Recombinant Rubella Virus Rubella E2 Protein, Untagged, E.coli-500ug
QP13374-500ug 500ug
EUR 795.6

Rubella virus E2 recombinant antigen
00216-V-01mg 0,1 mg
EUR 321
Description: Rubella virus E2 recombinant antigen a.a. 31-105.

Rubella virus E2 recombinant antigen
00216-V-1000ug 1000 ug
EUR 1539
Description: Rubella virus E2 recombinant antigen a.a. 31-105.

Rubella virus E1 recombinant antigen
8806 100 ug
EUR 529.8
Description: This is Rubella virus E1 recombinant antigen for ELISA,WB.

Rubella virus E2 recombinant antigen
8807 100 ug
EUR 529.8
Description: This is Rubella virus E2 recombinant antigen for ELISA,WB.

Rubella virus E2 Recombinant Protein
53-354 0.01 mg
EUR 714.3
Description: Rubella virus E2 Recombinant Protein

Rubella virus E1 Recombinant Protein
53-355 0.01 mg
EUR 714.3
Description: Rubella virus E1 Recombinant Protein

Recombinant Rubella Virus E2 Protein
DAG514 1mg
EUR 1537.2

Human Rubella Virus (RV) IgG Rapid Test Kit
abx092097-20tests 20 tests
EUR 260.4

Rubella Virus Capsid Protein Antibody
abx022208-1mg 1 mg
EUR 610.8

Recombinant Rubella Virus Capsid (33 kDa)
DAG494 1mg
EUR 1731.6

Goat Rubella virus antibody IgG ELSA KIT ELISA Kit
EGTR0019 96Tests
EUR 625.2

Goat Rubella virus antibody IgA ELSA KIT ELISA Kit
EGTR0020 96Tests
EUR 625.2

Goat Rubella virus antibody IgM ELSA KIT ELISA Kit
EGTR0021 96Tests
EUR 625.2

Recombinant (E.Coli) Rubella Virus E2 protein
RP-1414 100 ug
EUR 343.2

Rabbit Rubella virus antibody IgG ELSA KIT ELISA Kit
ERTR0019 96Tests
EUR 625.2

Rabbit Rubella virus antibody IgA ELSA KIT ELISA Kit
ERTR0020 96Tests
EUR 625.2

Rabbit Rubella virus antibody IgM ELSA KIT ELISA Kit
ERTR0021 96Tests
EUR 625.2

Bovine Rubella virus antibody IgG ELSA KIT ELISA Kit
EBR0019 96Tests
EUR 625.2

Bovine Rubella virus antibody IgA ELSA KIT ELISA Kit
EBR0020 96Tests
EUR 625.2

Bovine Rubella virus antibody IgM ELSA KIT ELISA Kit
EBR0021 96Tests
EUR 625.2

Canine Rubella virus antibody IgG ELSA KIT ELISA Kit
ECR0019 96Tests
EUR 625.2

Canine Rubella virus antibody IgA ELSA KIT ELISA Kit
ECR0020 96Tests
EUR 625.2

Canine Rubella virus antibody IgM ELSA KIT ELISA Kit
ECR0021 96Tests
EUR 625.2

Goat polyclonal antibody for Rubella virus
1701 1 ml
EUR 327.53
Description: This is goat polyclonal antibody against Rubella virus virions for WB, ELISA.

Goat polyclonal antibody for Rubella virus
1703 1 ml
EUR 347.1
Description: This is FITC conjugated goat polyclonal antibody against Rubella virus virions for WB, ELISA.

Goat polyclonal antibody for Rubella virus
1707 1 ml
EUR 347.1
Description: This is Biotin conjugated goat polyclonal antibody against Rubella virus virions for WB, ELISA.

Goat polyclonal antibody for Rubella virus
504 1 ml
EUR 373.2
Description: This is HRP conjugated goat polyclonal antibody against Rubella virus virions for WB, ELISA.

Porcine Rubella virus antibody IgG ELSA KIT ELISA Kit
EPR0019 96Tests
EUR 625.2

Porcine Rubella virus antibody IgA ELSA KIT ELISA Kit
EPR0020 96Tests
EUR 625.2

Porcine Rubella virus antibody IgM ELSA KIT ELISA Kit
EPR0021 96Tests
EUR 625.2

Rubella virus E1 mosaic recombinant antigen
00215-V-01mg 0,1 mg
EUR 321
Description: Rubella virus E1 mosaic recombinant antigen a.a. 157-176/374-390/213-239.

Rubella virus E1 mosaic recombinant antigen
00215-V-1000ug 1000 ug
EUR 1539
Description: Rubella virus E1 mosaic recombinant antigen a.a. 157-176/374-390/213-239.

Rubella virus capsid (C) recombinant antigen
00217-V-01mg 0,1 mg
EUR 321
Description: Rubella virus capsid (C) recombinant antigen a.a. 1-123.

Rubella virus capsid (C) recombinant antigen
00217-V-1000ug 1000 ug
EUR 1539
Description: Rubella virus capsid (C) recombinant antigen a.a. 1-123.

Anserine Rubella virus antibody IgG ELSA KIT ELISA Kit
EAR0019 96Tests
EUR 625.2

Anserine Rubella virus antibody IgA ELSA KIT ELISA Kit
EAR0020 96Tests
EUR 625.2

Anserine Rubella virus antibody IgM ELSA KIT ELISA Kit
EAR0021 96Tests
EUR 625.2

Mouse Anti Rubella Virus Monoclonal Antibody
DMABT-51535MR 0.2 mg
EUR 795.6

Mouse Rubella Virus Antibody IgG(RV-IgG )ELSA KIT
QY-E21685 96T
EUR 448.8

Recombinant Rubella Virus E1 mosaic (32 kDa)
DAG507 1mg
EUR 1725.6

Guinea Pig Rubella virus antibody IgG ELSA KIT ELISA Kit
EGR0019 96Tests
EUR 625.2

Guinea Pig Rubella virus antibody IgA ELSA KIT ELISA Kit
EGR0020 96Tests
EUR 625.2

Guinea Pig Rubella virus antibody IgM ELSA KIT ELISA Kit
EGR0021 96Tests
EUR 625.2

Rubella virus E2 recombinant antigen a.a. 31-105.
00216-V-100ugvial 100 ug/vial
EUR 150
Description: Rubella virus E2 recombinant antigen a.a. 31-105.

Mouse antibody for Rubella virus E1 glycoprotein
1712 100 ug
EUR 386.26
Description: This is purified Mouse monoclonal antibody against Rubella virus E1 glycoprotein for WB, ELISA.

Mouse antibody for Rubella virus E1 glycoprotein
1714 100 ug
EUR 386.26
Description: This is purified Mouse monoclonal antibody against Rubella virus E1 glycoprotein for WB, ELISA.

Mouse antibody for Rubella virus E1 glycoprotein
1715 100 ug
EUR 386.26
Description: This is purified Mouse monoclonal antibody against Rubella virus E1 glycoprotein for WB, ELISA.

Mouse antibody for Rubella virus E2 glycoprotein
1717 100 ug
EUR 386.26
Description: This is purified Mouse monoclonal antibody against Rubella virus E2 glycoprotein for WB, ELISA.

Recombinant Rubella Virus E1 Mosaic, His-tagged
DAG488 mg
EUR 1452

Recombinant (E.Coli) Rubella Virus E1 Mosaic protein
RP-1413 100 ug
EUR 343.2

Recombinant (E.Coli) Rubella Virus Capsid C protein
RP-1415 100 ug
EUR 343.2

QPCR Kit RNA Rubella virusMmix
MOL8314 EACH
EUR 1010.04

Rubella virus (HPV77 strain) proteins/antigens extract
RUBL15-N-500 0.5 ml
EUR 388.8

Rubella virus capsid (C) recombinant antigen a.a. 1-123.
00217-V-100ugvial 100 ug/vial
EUR 150
Description: Rubella virus capsid (C) recombinant antigen a.a. 1-123.

Mouse Anti Rubella Virus Capsid Protein Monoclonal Antibody
DMABT-51537MR 0.2 mg
EUR 920.4

Mouse Anti Rubella Virus Glycoprotein E1 Monoclonal Antibody
DMABT-51538MR 0.2 mg
EUR 795.6

Mouse Anti Rubella Virus Glycoprotein E2 Monoclonal Antibody
DMABT-51540MR 0.2 mg
EUR 920.4

Rubella virus E1 mosaic recombinant antigen a.a. 157-176/374-390/213-239.
00215-V-100ugvial 100 ug/vial
EUR 150
Description: Rubella virus E1 mosaic recombinant antigen a.a. 157-176/374-390/213-239.

RealScreen Rubella IgG ELISA Kit
EL030-096 96T
EUR 999.6

RealScreen Rubella IgM ELISA Kit
EL031-096 96T
EUR 854.4

Human Cytomegalovirus, Toxoplasma, Rubella, Herpes simplex virus type II IgM Rapid Test Kit
abx092077-20tests 20 tests
EUR 410.4

Rubella ELISA Kit
55R-RB025G 96 wells
EUR 361.2
Description: ELISA kit for detection of Rubella in the research laboratory

0.2ML CLEAR STRIP CAPS FOR REAL TIME PCR
PCR-2CP-RT-C 125/pk
EUR 267.6
Description: PCR Plates & Tubes; PCR Strip Tubes and Strip Caps - Axygen

Rubella IgG ELISA Kit
E4666-100 each
EUR 646.8

Rubella IgM ELISA Kit
E4667-100 each
EUR 646.8

Human Lung Cancer PCR Primer Library
HLUCPL-I 1 set
EUR 657.6

Human Schwann Cell PCR Primer Library
HSCH-I 1 set
EUR 657.6

Novel Coronavirus COVID-19 (2019-nCoV) Real Time RT-PCR Kit
RR-0478-02 25 tests/kit
EUR 1189.2
Description: Novel Coronavirus (2019-nCoV) Real Time RT-PCR Kit is used for the qualitative detection of a novel coronavirus, which was identified in 2019 at Wuhan City, Hubei Province, China, in upper respiratory tract specimens (nasopharyngeal extracts, deep cough sputum, etc.) and lower respiratory tract specimens (alveoli irrigation fluid, etc.) by real time PCR systems.

Rat Rubella IgG ELISA Kit
QY-E11851 96T
EUR 448.8

Rubella 2-42 Protein
abx069921-1mg 1 mg
EUR 610.8

Rabbit Rubella IgG
QY-E30313 96T
EUR 448.8

Rubella BR2S Antigen
30-1979 1 mg
EUR 1524

Rubella 2-6 Protein
abx069923-1mg 1 mg
EUR 610.8

Rubella IgM MAX Protein
abx160034-1mg 1 mg
EUR 4202.4

AzuraQuant cDNA Synthesis Kit - 100 Reactions
AZ-1996 100 Reactions
EUR 446.4

Axygen Polyester ultra clear sealing film for real time PCR
AXY2152 PK100
EUR 382.8

Eztime Real - time PCRPremix 2x,Taqman
FYT105-100P 100 Preps 1.25 ml Ask for price

Human Rubella IgG ELISA Kit (Direct EIA)
EK7082 96wells/kit, with removable strips.
EUR 573.6

Human Rubella IgM ELISA Kit (Direct EIA)
EK7083 96wells/kit, with removable strips.
EUR 573.6

AzuraQuant cDNA Synthesis Kit - 25 Reactions
AZ-1995 25 Reactions
EUR 183.6

Eco 48 Real Time PCR system including software and loading dock
MOL6348 EACH
EUR 18466.8

Eztime Real - time PCRPremix 2x,SYBR Green
FYT103-100P 100 Preps 1.25 ml Ask for price

Eztime Real - time PCRPremix 2x,SYBR Green
FYT103-400P 400 Preps 1.25 ml x 4 Ask for price

Eztime Real - time PCRPremix 2x,Taqman, ROX
FYT105-400P 400 Preps 1.25 ml x 4 Ask for price

Eztime Real - time PCRPremix 2x,Taqman, ROX
FYT106-100P 100 Preps 1.25 ml Ask for price

Eztime Real - time PCRPremix 2x,SYBRGreen , ROX
FYT104-100P 100 Preps 1.25 ml Ask for price

Eztime Real - time PCRPremix 2x,SYBRGreen , ROX
FYT104-400P 400 Preps 1.25 ml x 4 Ask for price

RV (Rubella Antibody IgM) ELISA test
31 96T/Box Ask for price
Description: ELISA based test for quantitative detection of RV (Rubella Antibody IgM)

RV (Rubella Antibody IgG) ELISA test
30 96T/Box Ask for price
Description: ELISA based test for quantitative detection of RV (Rubella Antibody IgG)

K Virus RT PCR kit
RTq-V365-100D 100T
EUR 860.4

K Virus RT PCR kit
RTq-V365-150D 150T
EUR 969.6
Finally, we demonstrate that rh-PCR primers provide a more homogeneous sample pool and greater sequence quality in a Next Generation Sequencing-based approach to obtaining DNA sequence information from large numbers of cloned antibody cognate pairs. Furthermore, the higher specificity of the rh-PCR primers allowed for a better match between native antibody germline sequences and the VL/VH fragments amplified from single B-cells.

Determination of the viability of Toxoplasma gondii oocysts by PCR real-time after treatment with propidium monoazide

 

This study aimed to investigate a methodology for discriminating viable and non-viable T. gondii oocysts in water. Analyses included two steps: (i) microscopic investigation with vital dyes; (ii) molecular investigation, using a real time PCR (qPCR), after parasite treatment (or not) with propidium monoazide (PMA).
The method was called qPCR-PMA. Oocyst aliquots were incubated (15 min) at 25 ºC or 100 ºC and analyzed by microscopy, after trypan blue and neutral red staining. Microscopic investigation determined viable and non-viable oocysts. For the molecular investigation, both aliquots of oocysts were treated with PMA. Non-viable oocysts, after PMA treatment, exhibited an inhibition of DNA amplification by qPCR. Although analyses were carried out with oocysts treated experimentally, these results suggest that qPCR-PMA can be a useful strategy to distinguish viable and non-viable T. gondii oocysts in water safety testing, showing if water is safe to drink.

Diagnostic Utility of a Custom 34-Gene Anchored Multiplex PCR-Based Next-Generation Sequencing Fusion Panel for the Diagnosis of Bone and Soft Tissue Neoplasms With Identification of Novel USP6 Fusion Partners in Aneurysmal Bone Cysts

 

Context.—: Bone and soft tissue tumors are heterogeneous, diagnostically challenging, and often defined by gene fusions.
Objective.—: To present our experience using a custom 34-gene targeted sequencing fusion panel.
Design.—: Total nucleic acid extracted from formalin-fixed, paraffin-embedded (FFPE) tumor specimens was subjected to open-ended, nested anchored multiplex polymerase chain reaction and enrichment of 34 gene targets, thus enabling detection of known and novel fusion partners.
Results.—: During a 12-month period, 145 patients were tested as part of routine clinical care. Tumor percentage ranged from 10% to 100% and turnaround time ranged from 3 to 15 (median, 7.9) days. The most common diagnostic groups were small round blue cell tumors, tumors of uncertain differentiation, fibroblastic/myofibroblastic tumors, and adipocytic tumors.
In-frame fusion transcripts were identified in 64 of 140 cases sequenced (46%): in 62 cases, the detection of a disease-defining fusion confirmed the morphologic impression; in 2 cases, a germline TFG-GPR128 polymorphic fusion variant was detected. Several genes in the panel partnered with multiple fusion partners specific for different diagnoses, for example, EWSR1, NR4A3, FUS, NCOA2, and TFE3. Interesting examples are presented to highlight how fusion detection or lack thereof was instrumental in establishing accurate diagnoses. Novel fusion partners were detected for 2 cases of solid aneurysmal bone cysts (PTBP1-USP6, SLC38A2-USP6).
Conclusions.—: Multiplex detection of fusions in total nucleic acid purified from FFPE specimens facilitates diagnosis of bone and soft tissue tumors. This technology is particularly useful for morphologically challenging entities and in the absence of prior knowledge of fusion partners, and has the potential to discover novel fusion partners.

Performance Evaluation of a Commercial Real-Time PCR Assay and of an In-House Real-Time PCR for Trypanosoma cruzi DNA Detection in a Tropical Medicine Reference Center, Northern Italy

 

Chagas disease, a neglected protozoal disease endemic in Latin America, is also currently considered an emerging threat in nonendemic areas because of population movements. The detection of Trypanosoma cruzi DNA is increasingly being considered as important evidence to support Chagas disease diagnoses.
However, further performance evaluation of molecular assays is useful for a standardization of strategy considering the whole process in routine diagnosis, especially for the different settings such as endemic and nonendemic countries. Seventy-five samples were collected from subjects screened for Chagas disease in Italy.
The DNA was isolated from blood using automated extraction. We evaluated the performance of the commercial RealCycler® CHAG kit (pmPCR) based on satellite DNA (SatDNA) and of an in-house real-time PCR (ihPCR) targeting Sat and kinetoplast (k) DNAs, using the concordance of two serology assays as a reference standard. The sensitivity of kDNA and SatDNA tests by ihPCR and SatDNA by pmPCR were 14.29% (95% confidence interval (CI) 6.38 to 26.22), 7.14% (95% CI 1.98 to 17.29), and 7.14% (95% CI 1.98 to 17.29), respectively.
Specificity was 100% for all PCR assays and targets. Overall, our results suggest that the preferred approach for clinical laboratories is to combine the kDNA and SatDNA as targets in order to minimize false-negative results increasing sensitivity.

Selection of DNA Aptamers for Subcellular Localization of RBSDV P10 Protein in the Midgut of Small Brown Planthoppers by Emulsion PCR-Based SELEX

Rice black-streaked dwarf virus (RBSDV), classified under the Reoviridae, Fijivirus genus, caused an epidemic in the eastern provinces of China and other East Asian countries and resulted in severe yield loss in rice and wheat production. RBSDV is transmitted by the small brown planthopper (SBPH, Laodelphax striatellus Fallén) in a persistent manner.
In order to provide a stable and cost-effective detection probe, in this study we selected three DNA aptamers (R3, R5 and R11) by an optimized, standardized and time saving emulsion PCR-based SELEX, for the detection of RBSDV outer-shell P10 protein for in situ localization studies in the midgut of SBPH. The specificity of these three DNA aptamers was tested through detection of the P10 protein using an enzyme-linked oligonucleotide assay (ELONA) and aptamer-based dot-blot ELISA.
All three DNA aptamers can be used to detect RBSDV P10 protein by immunofluorescent labeling in the midgut of RBSDV-infected SBPH. These data show that the selected aptamers can be used for the detection of RBSDV P10 protein in vitro and in vivo. This is the first report of aptamers being selected for detection of a rice virus capsid protein.

HCV Seroconversion Panel Donor# 63869 (6 X 1 mL)
HCV9043 6 X 1 mL
EUR 1035

HCV Seroconversion Panel Donor# 63911 (6 X 1 mL)
HCV9044 6 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 64150 (8 X 1 mL)
HCV9045 8 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 64254 (5 X 1 mL)
HCV9046 5 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 64273 (10 X 1 mL)
HCV9047 10 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 64437 (8 X 1 mL)
HCV9048 8 X 1 mL
EUR 1035

HCV Seroconversion Panel Donor# 1038851 (5 X 1 mL)
HCV9095 5 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 61025 (5 X 1 mL)
HCV6217 5 X 1 mL
EUR 1035

HCV Seroconversion Panel Donor# 61251 (8 X 1 mL)
HCV6218 8 X 1 mL
EUR 1035

HCV Seroconversion Panel Donor# 60749 (14 X 1 mL)
HCV6219 14 X 1 mL
EUR 1035

HCV Seroconversion Panel Donor# 63792 (6 X 1 mL)
HCV6220 6 X 1 mL
EUR 1035

HCV Seroconversion Panel Donor# 62258 (8 X 1 mL)
HCV6221 8 X 1 mL
EUR 1035

HCV Seroconversion Panel Donor# 62286 (8 X 1 mL)
HCV6222 8 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 62507 (22 X 1 mL)
HCV6223 22 X 1 mL
EUR 1035

HCV Seroconversion Panel Donor# 62680 (6 X 1 mL)
HCV6224 6 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 62999 (19 X 1 mL)
HCV6225 19 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 63135 (12 X 1 mL)
HCV6226 12 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 63318 (7 X 1 mL)
HCV6227 7 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 62886 (12 X 1 mL)
HCV6228 12 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 62804 (8 X 1 mL)
HCV6229 8 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 64766 (5 X 1 mL)
HCV9049 5 X 1 mL
EUR 1035

HCV Seroconversion Panel Donor# 66158 (13 X 1 mL)
HCV9050 13 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 66626 (8 X 1 mL)
HCV9054 8 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 66732 (11 X 1 mL)
HCV9055 11 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 66861 (11 X 1 mL)
HCV9057 11 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 66647 (5 X 1 mL)
HCV9058 5 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 69089 (10 X 1 mL)
HCV10000 10 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 69654 (7 X 1 mL)
HCV10002 7 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 69158 (13 X 1 mL)
HCV10003 13 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 69028 (6 X 1 mL)
HCV10004 6 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 67355 (11 X 1 mL)
HCV10007 11 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 69263 (10 X 1 mL)
HCV10008 10 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 68896 (6 X 1 mL)
HCV10009 6 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 65470 (7 X 1 mL)
HCV10010 7 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 66666 (13 X 1 mL)
HCV10011 13 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 66747 (13 X 1 mL)
HCV10012 13 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 67356 (10 X 1 mL)
HCV10013 10 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 68157 (4 X 1 mL)
HCV10014 4 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 68628 (12 X 1 mL)
HCV10016 12 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 65064 (16 X 1 mL)
HCV10017 16 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 69142 (8 X 1 mL)
HCV10019 8 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 68744 (13 X 1 mL)
HCV10020 13 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 68470 (14 X 1 mL)
HCV10021 14 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 68450 (11 X 1 mL)
HCV10022 11 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 67505 (21 X 1 mL)
HCV10023 21 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 66719 (8 X 1 mL)
HCV10024 8 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 66801 (11 X 1 mL)
HCV10025 11 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 67391 (12 X 1 mL)
HCV10026 12 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 66751 (8 X 1 mL)
HCV10027 8 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 67116 (7 X 1 mL)
HCV10028 7 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 67539 (14 X 1 mL)
HCV10029 16 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 65977 (8 X 1 mL)
HCV10030 8 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 66113 (16 X 1 mL)
HCV10032 16 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 68629 (10 X 1 mL)
HCV10033 10 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 68223 (13 X 1 mL)
HCV10036 13 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 65643 (10 X 1 mL)
HCV10043 10 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 65982 (4 X 1 mL)
HCV10044 4 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 66074 (5 X 1 mL)
HCV10045 5 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 67766 (15 X 1 mL)
HCV10050 15 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 67787 (14 X 1 mL)
HCV10051 14 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 67861 (12 X 1 mL)
HCV10052 12 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 420245049 (7 X 1 mL)
HCV10057 7 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 420299823 (7 X 1 mL)
HCV10058 7 X 1 mL
EUR 1858.37
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 490104584 (8 X 1 mL)
HCV10062 8 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 90163052 (7 X 1 mL)
HCV10071 7 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 72237 (9 X 1 mL)
HCV10165 9 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 70225 (5 X 1 mL)
HCV10185 5 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 22708D (5 X 1 mL)
HCV9094 5 X 1 mL
EUR 1764

HCV Pre/Post Seroconversion Panel Donor# 66822 (5 X 1 mL)
HCV10235 5 X 1 mL
EUR 545

Zika Virus Seroconversion Panel (7 X 0.5mL)
KZMC047 7 X 0.5mL
EUR 2890

HIV Seroconversion Panel Donor# 65404 (7 X mL)
HIV9013 7 X mL
EUR 1764

EBV Seroconversion Panel Donor# 283843421 (14 X 1 mL)
EBV9097 14 X 1 mL
EUR 1764

HIV Seroconversion Panel Donor# 73695 (9 X 1 mL)
HIV12007 9 X 1 mL
EUR 2344

HIV Seroconversion Panel Donor# 73698 (13 X 1 mL)
HIV12008 13 X 1 mL
EUR 2344

HIV Seroconversion Panel Donor# 63331 (7 X 1 mL)
HIV6248 7 X 1 mL
EUR 1764

HIV Seroconversion Panel Donor# 64954 (11 X 1 mL)
HIV9011 11 X 1 mL
EUR 1764

HIV Seroconversion Panel Donor# 65389 (8 X 1 mL)
HIV9012 8 X 1 mL
EUR 1764

HIV Seroconversion Panel Donor# 65522 (5 X 1 mL)
HIV9014 5 X 1 mL
EUR 1764

HIV Seroconversion Panel Donor# 65661 (6 X 1 mL)
HIV9015 6 X 1 mL
EUR 1858.37
Description: Please contact Gentaur in order to receive the datasheet of the product.

HIV Seroconversion Panel Donor# 65790 (10 X 1 mL)
HIV9016 10 X 1 mL
EUR 1764

HIV Seroconversion Panel Donor# 66575 (10 X 1 mL)
HIV9018 10 X 1 mL
EUR 1764

HIV Seroconversion Panel Donor# 65685 (3 X 1 mL)
HIV9019 3 X 1 mL
EUR 1764

HIV Seroconversion Panel Donor# 67512 (22 X 1 mL)
HIV9020 22 X 1 mL
EUR 1764

HIV Seroconversion Panel Donor# 67485 (17 X 1 mL)
HIV9021 17 X 1 mL
EUR 1764

HIV Seroconversion Panel Donor# 64578 (8 X 1 mL)
HIV9022 8 X 1 mL
EUR 1764

HIV Seroconversion Panel Donor# 67706 (22 X 1 mL)
HIV9023 22 X 1 mL
EUR 1764

HIV Seroconversion Panel Donor# 64469 (12 X 1 mL)
HIV9024 12 X 1 mL
EUR 1764

HIV Seroconversion Panel Donor# 67996 (12 X 1 mL)
HIV9025 12 X 1 mL
EUR 1764
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