Prospective case-control study of enterovirus detection differences in children’s cerebrospinal fluid between multiplex PCR and real-time RT-PCR assay
Background: Viral encephalitis is common in childhood. It is an acute brain parenchymal inflammation caused by a variety of viral infection, and enterovirus accounts for the majority. Due to atypical clinical manifestations, pathogenic testing is important for assisting clinical diagnosis. The purpose of this study was to evaluate the performance of the multiplex PCR assay compared with quantitative real-time PCR for enterovirus detection.
Methods: A prospective case-control study was performed involving 103 pediatric patients suspected for viral encephalitis and cerebrospinal fluid (CSF) samples were collected and tested for 9 pathogens using multiplex PCR assay during April to November in 2018. In parallel, an aliquot of samples was tested for enterovirus infection by real-time PCR assay.
mhealthinfo
Results: There were 85.4% children were confirmed as viral encephalitis on discharge, the remaining ones were diagnosed as other CNS diseases, such as epilepsy. The specificity of the two methods was the same as that of the clinical diagnosis, but the sensitivity and consistency with clinical diagnosis of multiplex PCR were both higher than the real-time PCR. Besides of enterovirus, multiplex PCR could also detect coinfection of enterovirus with Epstein-Barr virus and mumps virus.
Conclusion: Results of multiplex PCR method are more consistent with the clinical diagnosis and are superior to real-time PCR for detecting enterovirus in CSF.
Development of a Novel Competitive qRT-PCR Assay to Measure Relative Lentiviral Packaging Efficiency
Third-generation HIV-1-derived lentiviral vectors are successfully used as therapeutic agents in various clinical applications. To further promote their use, we attempted to enhance vector infectivity by targeting the dimerization and packaging properties of the RNA transfer vector based on the premise that these two processes are tightly linked.
We rationally designed mutant vectors to favor the dimeric conformation, potentially enhancing genome packaging. Initial assessments using standard assays generated outputs of variable reproducibility, sometimes with conflicting results. Therefore, we developed a novel competitive qRT-PCR assay in a co-transfection setting to measure the relative packaging efficiencies of wild-type and mutant transfer vectors. Here we report the effect of the dimerization-stabilizing mutations on infectious and physical titers of lentiviral vectors together with their packaging efficiency, measured using our novel assay.
GPR12 antibody |
70R-49804 |
Fitzgerald |
100 ul |
EUR 242 |
|
Description: Purified Polyclonal GPR12 antibody |
GPR12 Antibody |
1-CSB-PA008905 |
Cusabio |
-
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Description: A polyclonal antibody against GPR12. Recognizes GPR12 from Human. This antibody is Unconjugated. Tested in the following application: WB, IF, ELISA;WB:1/500-1/2000.IF:1/200-1/1000.ELISA:1/5000 |
GPR12 Antibody |
1-CSB-PA009742LA01HU |
Cusabio |
-
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Ask for price
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Description: A polyclonal antibody against GPR12. Recognizes GPR12 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
GPR12 Antibody |
MBS7105637-005mg |
MyBiosource |
0.05mg |
EUR 190 |
GPR12 Antibody |
MBS7105637-01mg |
MyBiosource |
0.1mg |
EUR 270 |
GPR12 Antibody |
MBS7105637-5x01mg |
MyBiosource |
5x0.1mg |
EUR 1205 |
GPR12 Antibody |
MBS7119453-005mg |
MyBiosource |
0.05mg |
EUR 150 |
GPR12 Antibody |
MBS7119453-01mg |
MyBiosource |
0.1mg |
EUR 190 |
GPR12 Antibody |
MBS7119453-5x01mg |
MyBiosource |
5x0.1mg |
EUR 845 |
GPR12 Antibody |
MBS9233167-01mL |
MyBiosource |
0.1mL |
EUR 415 |
GPR12 Antibody |
MBS9233167-5x01mL |
MyBiosource |
5x0.1mL |
EUR 1841 |
GPR12 Antibody |
MBS853434-01mg |
MyBiosource |
0.1mg |
EUR 305 |
GPR12 Antibody |
MBS853434-01mLAF405L |
MyBiosource |
0.1mL(AF405L) |
EUR 465 |
GPR12 Antibody |
MBS853434-01mLAF405S |
MyBiosource |
0.1mL(AF405S) |
EUR 465 |
GPR12 Antibody |
MBS853434-01mLAF610 |
MyBiosource |
0.1mL(AF610) |
EUR 465 |
GPR12 Antibody |
MBS853434-01mLAF635 |
MyBiosource |
0.1mL(AF635) |
EUR 465 |
GPR12 Antibody |
MBS5314424-01mL |
MyBiosource |
0.1mL |
EUR 470 |
GPR12 Antibody |
MBS5314424-5x01mL |
MyBiosource |
5x0.1mL |
EUR 1955 |
GPR12 antibody |
MBS535594-005mg |
MyBiosource |
0.05mg |
EUR 1125 |
GPR12 antibody |
MBS535594-5x005mg |
MyBiosource |
5x0.05mg |
EUR 4905 |
GPR12 Antibody |
MBS9604235-01mL |
MyBiosource |
0.1mL |
EUR 260 |
GPR12 Antibody |
MBS9604235-02mL |
MyBiosource |
0.2mL |
EUR 305 |
GPR12 Antibody |
MBS9604235-5x02mL |
MyBiosource |
5x0.2mL |
EUR 1220 |
GPR12 Antibody |
G323-100ul |
Assay Biotech |
100μl |
EUR 217 |
Description: GPR12 Rabbit Polyclonal Antibody |
GPR12 Antibody |
G323-50ul |
Assay Biotech |
50μl |
EUR 143.5 |
Description: GPR12 Rabbit Polyclonal Antibody |
GPR12 Antibody |
MBS9418304-005mL |
MyBiosource |
0.05mL |
EUR 245 |
GPR12 Antibody |
MBS9418304-01mL |
MyBiosource |
0.1mL |
EUR 305 |
GPR12 Antibody |
MBS9418304-5x01mL |
MyBiosource |
5x0.1mL |
EUR 1230 |
GPR12 Antibody |
MBS9508050-005mL |
MyBiosource |
0.05mL |
EUR 285 |
GPR12 Antibody |
MBS9508050-01mL |
MyBiosource |
0.1mL |
EUR 385 |
GPR12 Antibody |
MBS9508050-5x01mL |
MyBiosource |
5x0.1mL |
EUR 1590 |
GPR12 Polyclonal Antibody |
ABP54586-003ml |
Abbkine |
0.03ml |
EUR 189.6 |
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Description: A polyclonal antibody for detection of GPR12 from Human. This GPR12 antibody is for WB, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the N-terminal region of human GPR12 at AA rangle: 10-90 |
GPR12 Polyclonal Antibody |
ABP54586-01ml |
Abbkine |
0.1ml |
EUR 346.8 |
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Description: A polyclonal antibody for detection of GPR12 from Human. This GPR12 antibody is for WB, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the N-terminal region of human GPR12 at AA rangle: 10-90 |
GPR12 Polyclonal Antibody |
ABP54586-02ml |
Abbkine |
0.2ml |
EUR 496.8 |
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Description: A polyclonal antibody for detection of GPR12 from Human. This GPR12 antibody is for WB, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the N-terminal region of human GPR12 at AA rangle: 10-90 |
GPR12 Polyclonal Antibody |
E20-71963 |
EnoGene |
100ug |
EUR 225 |
Description: Available in various conjugation types. |
GPR12 Conjugated Antibody |
C44910 |
SAB |
100ul |
EUR 476.4 |
GPR12 Polyclonal Antibody |
BT-AP03685-100ul |
Jiaxing Korain Biotech Ltd (BT Labs) |
100ul |
Ask for price |
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Description: Promotes neurite outgrowth and blocks myelin inhibition in neurons (By similarity). Receptor with constitutive G(s) signaling activity that stimulates cyclic AMP production. |
GPR12 Polyclonal Antibody |
BT-AP03685-20ul |
Jiaxing Korain Biotech Ltd (BT Labs) |
20ul |
Ask for price |
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Description: Promotes neurite outgrowth and blocks myelin inhibition in neurons (By similarity). Receptor with constitutive G(s) signaling activity that stimulates cyclic AMP production. |
GPR12 Polyclonal Antibody |
BT-AP03685-50ul |
Jiaxing Korain Biotech Ltd (BT Labs) |
50ul |
Ask for price |
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Description: Promotes neurite outgrowth and blocks myelin inhibition in neurons (By similarity). Receptor with constitutive G(s) signaling activity that stimulates cyclic AMP production. |
GPR12 Polyclonal Antibody |
E44H04856 |
EnoGene |
100ul |
EUR 255 |
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody |
GPR12 Polyclonal Antibody |
MBS8522944-01mg |
MyBiosource |
0.1mg |
EUR 305 |
GPR12 Polyclonal Antibody |
MBS8522944-01mLAF405L |
MyBiosource |
0.1mL(AF405L) |
EUR 465 |
GPR12 Polyclonal Antibody |
MBS8522944-01mLAF405S |
MyBiosource |
0.1mL(AF405S) |
EUR 465 |
GPR12 Polyclonal Antibody |
MBS8522944-01mLAF610 |
MyBiosource |
0.1mL(AF610) |
EUR 465 |
GPR12 Polyclonal Antibody |
MBS8522944-01mLAF635 |
MyBiosource |
0.1mL(AF635) |
EUR 465 |
GPR12 Polyclonal Antibody |
MBS2559908-002mL |
MyBiosource |
0.02mL |
EUR 135 |
GPR12 Polyclonal Antibody |
MBS2559908-006mL |
MyBiosource |
0.06mL |
EUR 180 |
GPR12 Polyclonal Antibody |
MBS2559908-012mL |
MyBiosource |
0.12mL |
EUR 255 |
GPR12 Polyclonal Antibody |
MBS2559908-02mL |
MyBiosource |
0.2mL |
EUR 400 |
GPR12 Polyclonal Antibody |
MBS2559908-5x02mL |
MyBiosource |
5x0.2mL |
EUR 1715 |
GPR12 Conjugated Antibody |
MBS9452862-01mLAF350 |
MyBiosource |
0.1mL(AF350) |
EUR 480 |
GPR12 Conjugated Antibody |
MBS9452862-01mLAF405 |
MyBiosource |
0.1mL(AF405) |
EUR 480 |
GPR12 Conjugated Antibody |
MBS9452862-01mLAF488 |
MyBiosource |
0.1mL(AF488) |
EUR 480 |
GPR12 Conjugated Antibody |
MBS9452862-01mLAF555 |
MyBiosource |
0.1mL(AF555) |
EUR 480 |
GPR12 Conjugated Antibody |
MBS9452862-01mLBiotin |
MyBiosource |
0.1mL(Biotin) |
EUR 480 |
GPR12 Polyclonal Antibody |
UB-GEN-13085 |
UpingBio |
100 ul |
EUR 200 |
Anti- G Protein-Coupled Receptor GPR12 Antibody |
GWB-4852F8 |
GenWay Biotech |
0.05 mg |
Ask for price |
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GPR12 Antibody, HRP conjugated |
1-CSB-PA009742LB01HU |
Cusabio |
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Description: A polyclonal antibody against GPR12. Recognizes GPR12 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA |
GPR12 Antibody, HRP conjugated |
MBS7105638-005mg |
MyBiosource |
0.05mg |
EUR 190 |
GPR12 Antibody, HRP conjugated |
MBS7105638-01mg |
MyBiosource |
0.1mg |
EUR 270 |
GPR12 Antibody, HRP conjugated |
MBS7105638-5x01mg |
MyBiosource |
5x0.1mg |
EUR 1205 |
GPR12 Antibody, FITC conjugated |
1-CSB-PA009742LC01HU |
Cusabio |
-
Ask for price
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Description: A polyclonal antibody against GPR12. Recognizes GPR12 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA |
GPR12 Antibody, FITC conjugated |
MBS7105639-005mg |
MyBiosource |
0.05mg |
EUR 190 |
GPR12 Antibody, FITC conjugated |
MBS7105639-01mg |
MyBiosource |
0.1mg |
EUR 270 |
GPR12 Antibody, FITC conjugated |
MBS7105639-5x01mg |
MyBiosource |
5x0.1mg |
EUR 1205 |
GPR12 Antibody, Biotin conjugated |
1-CSB-PA009742LD01HU |
Cusabio |
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Ask for price
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Description: A polyclonal antibody against GPR12. Recognizes GPR12 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA |
GPR12 Antibody, Biotin conjugated |
MBS7105640-005mg |
MyBiosource |
0.05mg |
EUR 190 |
GPR12 Antibody, Biotin conjugated |
MBS7105640-01mg |
MyBiosource |
0.1mg |
EUR 270 |
GPR12 Antibody, Biotin conjugated |
MBS7105640-5x01mg |
MyBiosource |
5x0.1mg |
EUR 1205 |
GPR12 Rabbit Polyclonal Antibody |
ES5585-100ul |
ELK Biotech |
100ul |
EUR 124 |
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Description: A Rabbit Polyclonal antibody against GPR12 from Human. This antibody is tested and validated for WB, ELISA, IF, WB, ELISA |
GPR12 Rabbit Polyclonal Antibody |
ES5585-50ul |
ELK Biotech |
50ul |
EUR 74 |
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Description: A Rabbit Polyclonal antibody against GPR12 from Human. This antibody is tested and validated for WB, ELISA, IF, WB, ELISA |
GPR12 Rabbit Polyclonal Antibody |
E10G06593 |
EnoGene |
100 μl |
EUR 275 |
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody |
GPR12 Antibody - C-terminal region |
MBS3216517-01mL |
MyBiosource |
0.1mL |
EUR 455 |
GPR12 Antibody - C-terminal region |
MBS3216517-5x01mL |
MyBiosource |
5x0.1mL |
EUR 1995 |
GPR12 Antibody - N-terminal region |
MBS3222635-01mL |
MyBiosource |
0.1mL |
EUR 455 |
GPR12 Antibody - N-terminal region |
MBS3222635-5x01mL |
MyBiosource |
5x0.1mL |
EUR 1995 |
Polyclonal GPR12 Antibody (N-Terminus) |
APR16481G |
Leading Biology |
0.05mg |
EUR 580.8 |
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human GPR12 (N-Terminus). This antibody is tested and proven to work in the following applications: |
Polyclonal GPR12 Antibody (Extracellular Domain) |
APR16480G |
Leading Biology |
0.05mg |
EUR 580.8 |
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human GPR12 (Extracellular Domain). This antibody is tested and proven to work in the following applications: |
Polyclonal GPR12 Antibody - C-terminal region |
APR16482G |
Leading Biology |
0.05mg |
EUR 633.6 |
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human GPR12 - C-terminal region. This antibody is tested and proven to work in the following applications: |
G Protein Coupled Receptor 12 (GPR12) Antibody |
20-abx147559 |
Abbexa |
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G Protein Coupled Receptor 12 (GPR12) Antibody |
20-abx009208 |
Abbexa |
-
Ask for price
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G Protein Coupled Receptor 12 (GPR12) Antibody |
20-abx338465 |
Abbexa |
-
Ask for price
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- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
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G Protein Coupled Receptor 12 (GPR12) Antibody |
20-abx324375 |
Abbexa |
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Ask for price
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G Protein Coupled Receptor 12 (GPR12) Antibody |
abx147559-1096tests |
Abbexa |
10 × 96 tests |
Ask for price |
G Protein Coupled Receptor 12 (GPR12) Antibody |
abx147559-596tests |
Abbexa |
5 × 96 tests |
EUR 387.5 |
G Protein Coupled Receptor 12 (GPR12) Antibody |
abx147559-96tests |
Abbexa |
96 tests |
EUR 300 |
G Protein Coupled Receptor 12 (GPR12) Antibody |
abx338465-100g |
Abbexa |
100 µg |
EUR 162.5 |
G Protein Coupled Receptor 12 (GPR12) Antibody |
abx324375-100g |
Abbexa |
100 µg |
EUR 250 |
G Protein Coupled Receptor 12 (GPR12) Antibody |
abx324375-50g |
Abbexa |
50 µg |
EUR 187.5 |
G Protein-Coupled Receptor GPR12 Rabbit anti-Human Polyclonal Antibody |
GWB-1C7874 |
GenWay Biotech |
0.05 mg |
Ask for price |
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ARP64695_P050-25UL - GPR12 Antibody - C-terminal region |
ARP64695_P050-25UL |
Aviva Systems Biology |
25ul |
EUR 99 |
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ARP85321_P050-25UL - GPR12 Antibody - N-terminal region |
ARP85321_P050-25UL |
Aviva Systems Biology |
25ul |
EUR 99 |
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ARP64695_P050 - GPR12 Antibody - C-terminal region (ARP64695_P050) |
ARP64695_P050 |
Aviva Systems Biology |
100ul |
EUR 389 |
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ARP85321_P050 - GPR12 Antibody - N-terminal region (ARP85321_P050) |
ARP85321_P050 |
Aviva Systems Biology |
100ul |
EUR 389 |
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G Protein Coupled Receptor 12 (GPR12) Antibody (HRP) |
20-abx335850 |
Abbexa |
-
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- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
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G Protein Coupled Receptor 12 (GPR12) Antibody (HRP) |
abx335850-100g |
Abbexa |
100 µg |
EUR 362.5 |
G Protein Coupled Receptor 12 (GPR12) Antibody (HRP) |
abx335850-20g |
Abbexa |
20 µg |
EUR 162.5 |
G Protein Coupled Receptor 12 (GPR12) Antibody (HRP) |
abx335850-50g |
Abbexa |
50 µg |
EUR 250 |
G Protein Coupled Receptor 12 (GPR12) Antibody (FITC) |
20-abx335851 |
Abbexa |
-
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- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
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G Protein Coupled Receptor 12 (GPR12) Antibody (FITC) |
abx335851-100g |
Abbexa |
100 µg |
EUR 362.5 |
G Protein Coupled Receptor 12 (GPR12) Antibody (FITC) |
abx335851-20g |
Abbexa |
20 µg |
EUR 162.5 |
G Protein Coupled Receptor 12 (GPR12) Antibody (FITC) |
abx335851-50g |
Abbexa |
50 µg |
EUR 250 |
G Protein Coupled Receptor 12 (GPR12) Antibody (Biotin) |
20-abx335852 |
Abbexa |
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- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
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G Protein Coupled Receptor 12 (GPR12) Antibody (Biotin) |
abx335852-100g |
Abbexa |
100 µg |
EUR 362.5 |
G Protein Coupled Receptor 12 (GPR12) Antibody (Biotin) |
abx335852-20g |
Abbexa |
20 µg |
EUR 162.5 |
Enhancing dimerization did not automatically lead to better vector RNA packaging, suggesting that, for vector functionality, sufficient flexibility of the RNA to adopt different conformations is more important than the dimerization capacity. Our novel competitive qPCR assay enables a more stringent analysis of RNA packaging efficiency, allowing a much more precise understanding of the links between RNA structure, packaging, and infectious titers that will be invaluable for future vector development.
At what times during infection is SARS-CoV-2 detectable and no longer detectable using RT-PCR-based tests? A systematic review of individual participant data
Background: Tests for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viral ribonucleic acid (RNA) using reverse transcription polymerase chain reaction (RT-PCR) are pivotal to detecting current coronavirus disease (COVID-19) and duration of detectable virus indicating potential for infectivity.
Methods: We conducted an individual participant data (IPD) systematic review of longitudinal studies of RT-PCR test results in symptomatic SARS-CoV-2. We searched PubMed, LitCOVID, medRxiv, and COVID-19 Living Evidence databases. We assessed risk of bias using a QUADAS-2 adaptation. Outcomes were the percentage of positive test results by time and the duration of detectable virus, by anatomical sampling sites.
Results: Of 5078 studies screened, we included 32 studies with 1023 SARS-CoV-2 infected participants and 1619 test results, from – 6 to 66 days post-symptom onset and hospitalisation. The highest percentage virus detection was from nasopharyngeal sampling between 0 and 4 days post-symptom onset at 89% (95% confidence interval (CI) 83 to 93) dropping to 54% (95% CI 47 to 61) after 10 to 14 days. On average, duration of detectable virus was longer with lower respiratory tract (LRT) sampling than upper respiratory tract (URT). Duration of faecal and respiratory tract virus detection varied greatly within individual participants. In some participants, virus was still detectable at 46 days post-symptom onset.
Conclusions: RT-PCR misses detection of people with SARS-CoV-2 infection; early sampling minimises false negative diagnoses. Beyond 10 days post-symptom onset, lower RT or faecal testing may be preferred sampling sites. The included studies are open to substantial risk of bias, so the positivity rates are probably overestimated.
A real-time PCR assay to detect predation by spiny dogfish on Atlantic cod in the western North Atlantic Ocean
Conventional observations show spiny dogfish (Squalus acanthius Linnaeus) rarely eat Atlantic cod (Gadus morhua Linnaeus; 0.02% of stomachs) in the northwestern Atlantic Ocean. Critics express concern that digestion may limit species-level prey identification, and with recovery from overfishing, dogfish populations may be suppressing cod by competition or predation.
This study applied a real-time PCR TaqMan assay to identify cod in dogfish stomachs collected by cooperating fishing boats during normal trawling operations (May 2014-May 2015; Gulf of Maine, Georges Bank). Conventional methods observed 51 different prey taxa and nearly 1,600 individual prey items, but no cod were observed. Cod DNA was detected in 31 (10.5%) of the dogfish stomachs, with a higher percentage of these from the homogenate of amorphous, well-digested prey and stomach fluids (20 stomachs or 65%) than from discrete animal tissues (11 stomachs or 35%). Re-examination of photographs of these 11 tissue samples revealed one whole, partially digested fish that could be recognized in hindsight as cod.
Cod DNA was observed in dogfish stomachs year round: in January (1 of 1 trip), February (1 of 1), May (1 of 3), June (0 of 1), July (3 of 4), August (1 of 2), and October (3 of 3). Although these data suggest higher interaction rates between dogfish and cod than previously observed, addressing the population consequences of this predator-prey relationship requires a robust sampling design, estimates of digestion rates by dogfish to account for complete degradation of DNA sequences, and consideration for dogfish scavenging during fishing operations.
Multiple PCR assay based on the cigR gene for detection of Salmonella spp. and Salmonella Pullorum/Gallinarum identification
Salmonella spp. are important zoonotic pathogens that are responsible for severe diseases in both animals and humans. Salmonella enterica subsp. enterica serovar Gallinarum biovar Gallinarum (S. Gallinarum) and biovar Pullorum (S. Pullorum) are typical infectious pathogens detected in the chicken industry that have caused great economic losses. To facilitate their detection and prevent contamination, we developed a rapid multiple PCR method, which can simultaneously detect Salmonella spp. and further identify the biovars S. Pullorum/Gallinarum. This PCR detection method is based on the cigR gene, which is conserved among Salmonella spp. but has a 42-bp deletion in S. Pullorum/Gallinarum. The specificity and sensitivity of the PCR assay was evaluated with 41 different strains: 34 Salmonella strains, including 5 S. Pullorum/Gallinarum strains, and 7 non-Salmonella strains.
The lower limit of detection was 8.15 pg of S. Pullorum (S06004) genomic DNA and 20 cfu in PCR, which shows a great sensitivity. In addition, this method was applied to detect or identify Salmonella from processing chicken liver and egg samples, and the results corresponded to those obtained from serotype analysis using the conventional slide agglutination test. Overall, the new cigR-based PCR assay is efficient and practical for Salmonella detection and S. Pullorum/Gallinarum identification and will greatly reduce the workload of epidemiologic investigation.
Monkeypox Virus Real Time PCR Kit |
PDPS-AR064 |
Creative Biogene |
1 unit |
Ask for price |
Description: Creative Biogene Monkeypox Virus Real Time PCR Kit is used for the detection of monkeypox Virus in serum or lesion exudate samples by using real time PCR systems. Monkeypox virus (MPV) is a double-stranded DNA, zoonotic virus and a species of the genus Orthopoxvirus in the family Poxviridae. It is one of the human orthopoxviruses that includes variola (VARV), cowpox (CPX), and vaccinia (VACV) viruses. The kit contains a specific ready-to-use system for the detection of the monkeypox Virus. Fluorescence is emitted and measured by the real time systems' optical unit during the PCR. |
Virus Zika RT PCR kit |
RTq-H434-100R |
Bioingentech |
100T |
EUR 1573.2 |
Virus Zika RT PCR kit |
RTq-H434-150R |
Bioingentech |
150T |
EUR 2144.4 |
Virus Zika RT PCR kit |
RTq-H434-50R |
Bioingentech |
50T |
EUR 1155.6 |
Measles Virus and Rubella Virus Real Time RT-PCR Kit |
GWB-LRB047 |
GenWay Biotech |
25 tests |
Ask for price |
Measles Virus and Rubella Virus Real Time RT-PCR Kit |
GWB-LRB048 |
GenWay Biotech |
25 tests |
Ask for price |
Zika virus (ZIKV) (strain Zika SPH2015) ZIKV-NS1 ELISA Pair Set |
MBS8118462-15Plates |
MyBiosource |
15Plates |
EUR 800 |
Zika virus (ZIKV) (strain Zika SPH2015) ZIKV-NS1 ELISA Pair Set |
MBS8118462-5Plates |
MyBiosource |
5Plates |
EUR 450 |
Zika virus (ZIKV) (strain Zika SPH2015) ZIKV-NS1 ELISA Pair Set |
MBS8118462-5x15Plates |
MyBiosource |
5x15Plates |
EUR 3695 |
Canine Distemper virus Real Time RT-PCR Detection kit |
TRI-L10M1 |
TRI Biotech |
32T |
EUR 705.6 |
Canine Distemper virus Real Time RT-PCR Detection kit |
TRI-L10S1 |
TRI Biotech |
16T |
EUR 378 |
Zika Virus (ZIKV-Env) Antibody |
MBS568015-1mg |
MyBiosource |
1mg |
EUR 650 |
Zika Virus (ZIKV-Env) Antibody |
MBS568015-5x1mg |
MyBiosource |
5x1mg |
EUR 2735 |
Zika Virus (ZIKV-NS1) Antibody |
MBS5680298-1mg |
MyBiosource |
1mg |
EUR 650 |
Zika Virus (ZIKV-NS1) Antibody |
MBS5680298-5x1mg |
MyBiosource |
5x1mg |
EUR 2735 |
Zika Virus (ZIKV-NS1) Antibody |
MBS5680299-1mg |
MyBiosource |
1mg |
EUR 650 |
Zika Virus (ZIKV-NS1) Antibody |
MBS5680299-5x1mg |
MyBiosource |
5x1mg |
EUR 2735 |
Zika Virus (ZIKV-NS1) Antibody |
MBS5680300-1mg |
MyBiosource |
1mg |
EUR 650 |
Zika Virus (ZIKV-NS1) Antibody |
MBS5680300-5x1mg |
MyBiosource |
5x1mg |
EUR 2735 |
Zika Virus (ZIKV-NS1) Antibody |
MBS5680301-1mg |
MyBiosource |
1mg |
EUR 650 |
Zika Virus (ZIKV-NS1) Antibody |
MBS5680301-5x1mg |
MyBiosource |
5x1mg |
EUR 2735 |
Zika Virus (ZIKV-NS1) Antibody |
MBS5680302-1mg |
MyBiosource |
1mg |
EUR 650 |
Zika Virus (ZIKV-NS1) Antibody |
MBS5680302-5x1mg |
MyBiosource |
5x1mg |
EUR 2735 |
Zika Virus (ZIKV-NS1) Antibody |
MBS568046-1mg |
MyBiosource |
1mg |
EUR 650 |
Zika Virus (ZIKV-NS1) Antibody |
MBS568046-5x1mg |
MyBiosource |
5x1mg |
EUR 2735 |
Zika Virus (ZIKV-Env) Antibody |
MBS568050-1mg |
MyBiosource |
1mg |
EUR 650 |
Zika Virus (ZIKV-Env) Antibody |
MBS568050-5x1mg |
MyBiosource |
5x1mg |
EUR 2735 |
Zika Virus (ZIKV-Env) Antibody |
MBS568061-1mg |
MyBiosource |
1mg |
EUR 650 |
Zika Virus (ZIKV-Env) Antibody |
MBS568061-5x1mg |
MyBiosource |
5x1mg |
EUR 2735 |
Zika Virus (ZIKV-NS1) Antibody |
MBS568107-1mg |
MyBiosource |
1mg |
EUR 650 |
Zika Virus (ZIKV-NS1) Antibody |
MBS568107-5x1mg |
MyBiosource |
5x1mg |
EUR 2735 |
Zika Virus (ZIKV-NS1) Antibody |
MBS568181-1mg |
MyBiosource |
1mg |
EUR 650 |
Zika Virus (ZIKV-NS1) Antibody |
MBS568181-5x1mg |
MyBiosource |
5x1mg |
EUR 2735 |
Zika Virus (ZIKV-NS1) Antibody |
MBS568251-1mg |
MyBiosource |
1mg |
EUR 650 |
Zika Virus (ZIKV-NS1) Antibody |
MBS568251-5x1mg |
MyBiosource |
5x1mg |
EUR 2735 |
Zika Virus (ZIKV-Env) Antibody |
MBS568312-1mg |
MyBiosource |
1mg |
EUR 650 |
Zika Virus (ZIKV-Env) Antibody |
MBS568312-5x1mg |
MyBiosource |
5x1mg |
EUR 2735 |
Zika Virus (ZIKV-NS1) Antibody |
MBS568341-1mg |
MyBiosource |
1mg |
EUR 650 |
Zika Virus (ZIKV-NS1) Antibody |
MBS568341-5x1mg |
MyBiosource |
5x1mg |
EUR 2735 |
Zika Virus (ZIKV-NS1) Antibody |
MBS568350-1mg |
MyBiosource |
1mg |
EUR 650 |
Zika Virus (ZIKV-NS1) Antibody |
MBS568350-5x1mg |
MyBiosource |
5x1mg |
EUR 2735 |
Zika Virus (ZIKV-NS1) Antibody |
MBS568389-1mg |
MyBiosource |
1mg |
EUR 650 |
Zika Virus (ZIKV-NS1) Antibody |
MBS568389-5x1mg |
MyBiosource |
5x1mg |
EUR 2735 |
Zika virus (ZIKV) (strain Zika SPH2015) ZIKV-NS1 Antibody, Mouse MAb |
MBS8110733-002mL |
MyBiosource |
0.02mL |
EUR 185 |
Zika virus (ZIKV) (strain Zika SPH2015) ZIKV-NS1 Antibody, Mouse MAb |
MBS8110733-01mL |
MyBiosource |
0.1mL |
EUR 365 |
Zika virus (ZIKV) (strain Zika SPH2015) ZIKV-NS1 Antibody, Mouse MAb |
MBS8110733-5x01mL |
MyBiosource |
5x0.1mL |
EUR 1490 |
Zika virus (ZIKV) (strain Zika SPH2015) ZIKV-NS1 Antibody, Mouse MAb |
MBS8110734-002mL |
MyBiosource |
0.02mL |
EUR 185 |
Zika virus (ZIKV) (strain Zika SPH2015) ZIKV-NS1 Antibody, Mouse MAb |
MBS8110734-01mL |
MyBiosource |
0.1mL |
EUR 365 |
Zika virus (ZIKV) (strain Zika SPH2015) ZIKV-NS1 Antibody, Mouse MAb |
MBS8110734-5x01mL |
MyBiosource |
5x0.1mL |
EUR 1490 |
Zika virus (ZIKV) (strain Zika SPH2015) ZIKV-NS1 Antibody, Mouse MAb |
MBS8110736-002mL |
MyBiosource |
0.02mL |
EUR 185 |
Zika virus (ZIKV) (strain Zika SPH2015) ZIKV-NS1 Antibody, Mouse MAb |
MBS8110736-01mL |
MyBiosource |
0.1mL |
EUR 365 |
Zika virus (ZIKV) (strain Zika SPH2015) ZIKV-NS1 Antibody, Mouse MAb |
MBS8110736-5x01mL |
MyBiosource |
5x0.1mL |
EUR 1490 |
Zika virus (ZIKV) (strain Zika SPH2015) ZIKV-NS1 Antibody, Rabbit MAb |
MBS8110737-002mL |
MyBiosource |
0.02mL |
EUR 185 |
Zika virus (ZIKV) (strain Zika SPH2015) ZIKV-NS1 Antibody, Rabbit MAb |
MBS8110737-01mL |
MyBiosource |
0.1mL |
EUR 385 |
Zika virus (ZIKV) (strain Zika SPH2015) ZIKV-NS1 Antibody, Rabbit MAb |
MBS8110737-5x01mL |
MyBiosource |
5x0.1mL |
EUR 1590 |
Transfusion-transmitted Virus (TTV) Real Time PCR Kit |
GWB-LRB041 |
GenWay Biotech |
25 tests |
Ask for price |
Transfusion-transmitted Virus (TTV) Real Time PCR Kit |
GWB-LRB042 |
GenWay Biotech |
25 tests |
Ask for price |
Yellow head Virus Real Time PCR Detection Kit |
TRI-N08M1 |
TRI Biotech |
32T |
EUR 453.6 |
Yellow head Virus Real Time PCR Detection Kit |
TRI-N08S1 |
TRI Biotech |
16T |
EUR 252 |
Taura Syndrome Virus Real Time PCR Detection Kit |
TRI-N07M1 |
TRI Biotech |
32T |
EUR 453.6 |
Taura Syndrome Virus Real Time PCR Detection Kit |
TRI-N07S1 |
TRI Biotech |
16T |
EUR 252 |
Feline leukemia virus Real Time PCR Detection kit |
TRI-L23M1 |
TRI Biotech |
32T |
EUR 705.6 |
Feline leukemia virus Real Time PCR Detection kit |
TRI-L23S1 |
TRI Biotech |
16T |
EUR 378 |
Zika virus (ZIKV) (strain Zika SPH2015) ZIKV-NS1 Antibody (HRP), Mouse MAb |
MBS8110735-01mg |
MyBiosource |
0.1mg |
EUR 440 |
Zika virus (ZIKV) (strain Zika SPH2015) ZIKV-NS1 Antibody (HRP), Mouse MAb |
MBS8110735-5x01mg |
MyBiosource |
5x0.1mg |
EUR 1825 |
Zika virus (strain Zika SPH2015) ZIKV-NS1 ELISA Kit |
MBS8123983-5x96Tests |
MyBiosource |
5x96Tests |
EUR 2995 |
Zika virus (strain Zika SPH2015) ZIKV-NS1 ELISA Kit |
MBS8123983-96Tests |
MyBiosource |
96Tests |
EUR 655 |
ELISA kit for Human Zika Virus (ZIKV) Kit |
KTE62982-48T |
Abbkine |
48T |
EUR 424.8 |
Description: Quantitative sandwich ELISA for measuring Human Zika Virus (ZIKV) Kit in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
ELISA kit for Human Zika Virus (ZIKV) Kit |
KTE62982-5platesof96wells |
Abbkine |
5 plates of 96 wells |
EUR 2702.4 |
Description: Quantitative sandwich ELISA for measuring Human Zika Virus (ZIKV) Kit in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
ELISA kit for Human Zika Virus (ZIKV) Kit |
KTE62982-96T |
Abbkine |
96T |
EUR 686.4 |
Description: Quantitative sandwich ELISA for measuring Human Zika Virus (ZIKV) Kit in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
Dengue Real-TM
Real Time PCR kit for detection of Dengue Virus |
V63-S-50FRT |
Sacace Biotechnologies |
50 |
EUR 627.84 |
Viral Hemorrhagic Septicemia Virus Real Time RT-PCR Detection kit |
TRI-N16M1 |
TRI Biotech |
32T |
EUR 577.08 |
Viral Hemorrhagic Septicemia Virus Real Time RT-PCR Detection kit |
TRI-N16S1 |
TRI Biotech |
16T |
EUR 327.6 |
White Spot Syndrome Virus Real Time PCR Detection Kit |
TRI-N01M1 |
TRI Biotech |
32T |
EUR 453.6 |
White Spot Syndrome Virus Real Time PCR Detection Kit |
TRI-N01S1 |
TRI Biotech |
16T |
EUR 252 |
Zika virus (ZIKV) (strain Zika SPH2015) ZIKV-E/Envelope Protein Antibody, Mouse MAb |
MBS8110727-002mL |
MyBiosource |
0.02mL |
EUR 185 |
Zika virus (ZIKV) (strain Zika SPH2015) ZIKV-E/Envelope Protein Antibody, Mouse MAb |
MBS8110727-01mL |
MyBiosource |
0.1mL |
EUR 365 |
Zika virus (ZIKV) (strain Zika SPH2015) ZIKV-E/Envelope Protein Antibody, Mouse MAb |
MBS8110727-5x01mL |
MyBiosource |
5x0.1mL |
EUR 1490 |
Virus Zika PCR kit |
PCR-H434-96R |
Bioingentech |
100T |
EUR 1335.6 |
Macrobrachium rosenbergii Norda Virus Real Time RT-PCR Detection kit |
TRI-N13M1 |
TRI Biotech |
32T |
EUR 577.08 |
Macrobrachium rosenbergii Norda Virus Real Time RT-PCR Detection kit |
TRI-N13S1 |
TRI Biotech |
16T |
EUR 327.6 |
Anti-Zika virus(ZIKV)(strain Zika SPH2015) ZIKV-NS1 Monoclonal Antibody |
E-AB-V1336-100uL |
Elabscience Biotech |
100uL |
EUR 415 |
|
Description: Unconjugated |
Anti-Zika virus(ZIKV)(strain Zika SPH2015) ZIKV-NS1 Monoclonal Antibody |
E-AB-V1336-50uL |
Elabscience Biotech |
50uL |
EUR 250 |
|
Description: Unconjugated |
Anti-Zika virus(ZIKV)(strain Zika SPH2015) ZIKV-NS1 Monoclonal Antibody |
E-AB-V1336-each |
Elabscience Biotech |
each |
Ask for price |
|
Description: Unconjugated |
Anti-Zika virus(ZIKV)(strain Zika SPH2015) ZIKV-NS1 Monoclonal Antibody |
E-AB-V1337-100uL |
Elabscience Biotech |
100uL |
EUR 415 |
|
Description: Unconjugated |
Anti-Zika virus(ZIKV)(strain Zika SPH2015) ZIKV-NS1 Monoclonal Antibody |
E-AB-V1337-50uL |
Elabscience Biotech |
50uL |
EUR 250 |
|
Description: Unconjugated |
Anti-Zika virus(ZIKV)(strain Zika SPH2015) ZIKV-NS1 Monoclonal Antibody |
E-AB-V1337-each |
Elabscience Biotech |
each |
Ask for price |
|
Description: Unconjugated |
Anti-Zika virus(ZIKV)(strain Zika SPH2015) ZIKV-NS1 Monoclonal Antibody |
E-AB-V1338-100uL |
Elabscience Biotech |
100uL |
EUR 415 |
|
Description: Unconjugated |
Anti-Zika virus(ZIKV)(strain Zika SPH2015) ZIKV-NS1 Monoclonal Antibody |
E-AB-V1338-50uL |
Elabscience Biotech |
50uL |
EUR 250 |
|
Description: Unconjugated |
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