A real-time PCR assay to detect predation by spiny dogfish on Atlantic cod in the western North Atlantic Ocean

mhealthinfo

Prospective case-control study of enterovirus detection differences in children’s cerebrospinal fluid between multiplex PCR and real-time RT-PCR assay

Background: Viral encephalitis is common in childhood. It is an acute brain parenchymal inflammation caused by a variety of viral infection, and enterovirus accounts for the majority. Due to atypical clinical manifestations, pathogenic testing is important for assisting clinical diagnosis. The purpose of this study was to evaluate the performance of the multiplex PCR assay compared with quantitative real-time PCR for enterovirus detection.
Methods: A prospective case-control study was performed involving 103 pediatric patients suspected for viral encephalitis and cerebrospinal fluid (CSF) samples were collected and tested for 9 pathogens using multiplex PCR assay during April to November in 2018. In parallel, an aliquot of samples was tested for enterovirus infection by real-time PCR assay.
mhealthinfo

mhealthinfo

Results: There were 85.4% children were confirmed as viral encephalitis on discharge, the remaining ones were diagnosed as other CNS diseases, such as epilepsy. The specificity of the two methods was the same as that of the clinical diagnosis, but the sensitivity and consistency with clinical diagnosis of multiplex PCR were both higher than the real-time PCR. Besides of enterovirus, multiplex PCR could also detect coinfection of enterovirus with Epstein-Barr virus and mumps virus.
Conclusion: Results of multiplex PCR method are more consistent with the clinical diagnosis and are superior to real-time PCR for detecting enterovirus in CSF.

Development of a Novel Competitive qRT-PCR Assay to Measure Relative Lentiviral Packaging Efficiency

Third-generation HIV-1-derived lentiviral vectors are successfully used as therapeutic agents in various clinical applications. To further promote their use, we attempted to enhance vector infectivity by targeting the dimerization and packaging properties of the RNA transfer vector based on the premise that these two processes are tightly linked.
We rationally designed mutant vectors to favor the dimeric conformation, potentially enhancing genome packaging. Initial assessments using standard assays generated outputs of variable reproducibility, sometimes with conflicting results. Therefore, we developed a novel competitive qRT-PCR assay in a co-transfection setting to measure the relative packaging efficiencies of wild-type and mutant transfer vectors. Here we report the effect of the dimerization-stabilizing mutations on infectious and physical titers of lentiviral vectors together with their packaging efficiency, measured using our novel assay.

GPR12 Antibody

ABD2721 100 ug
EUR 438.00

GPR12 Antibody

44910-100ul 100ul
EUR 252.00

GPR12 Antibody

44910-50ul 50ul
EUR 187.00

GPR12 antibody

20R-GR041 50 ug
EUR 656.00
Description: Rabbit polyclonal GPR12 antibody

GPR12 Antibody

DF2721 200ul
EUR 304.00
Description: GPR12 antibody detects endogenous levels of total GPR12.

GPR12 Antibody

1-CSB-PA008905
  • EUR 222.00
  • EUR 195.00
  • 0
  • 1
  • Form: Liquid
  • Buffer: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Description: A polyclonal antibody against GPR12. Recognizes GPR12 from Human. This antibody is Unconjugated. Tested in the following application: WB, IF, ELISA;WB:1/500-1/2000.IF:1/200-1/1000.ELISA:1/5000

GPR12 Antibody

1-CSB-PA009742LA01HU
  • EUR 317.00
  • EUR 335.00
  • 0
  • 1
  • Form: Liquid
  • Buffer: Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against GPR12. Recognizes GPR12 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200

GPR12

RA30050 50 ug
EUR 618.00

Gpr12/ Rat Gpr12 ELISA Kit

ELI-09764r 96 Tests
EUR 886.00

GPR12 Conjugated Antibody

C44910 100ul
EUR 397.00

GPR12 Polyclonal Antibody

ES5585-100ul 100ul
EUR 279.00
Description: A Rabbit Polyclonal antibody against GPR12 from Human. This antibody is tested and validated for WB, ELISA, IF, WB, ELISA

GPR12 Polyclonal Antibody

ES5585-50ul 50ul
EUR 207.00
Description: A Rabbit Polyclonal antibody against GPR12 from Human. This antibody is tested and validated for WB, ELISA, IF, WB, ELISA

GPR12 Polyclonal Antibody

ABP54586-003ml 0.03ml
EUR 158.00
  • Immunogen information: Synthesized peptide derived from the N-terminal region of human GPR12 at AA rangle: 10-90
  • Applications tips:
Description: A polyclonal antibody for detection of GPR12 from Human. This GPR12 antibody is for WB, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the N-terminal region of human GPR12 at AA rangle: 10-90

GPR12 Polyclonal Antibody

ABP54586-01ml 0.1ml
EUR 289.00
  • Immunogen information: Synthesized peptide derived from the N-terminal region of human GPR12 at AA rangle: 10-90
  • Applications tips:
Description: A polyclonal antibody for detection of GPR12 from Human. This GPR12 antibody is for WB, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the N-terminal region of human GPR12 at AA rangle: 10-90

GPR12 Polyclonal Antibody

ABP54586-02ml 0.2ml
EUR 414.00
  • Immunogen information: Synthesized peptide derived from the N-terminal region of human GPR12 at AA rangle: 10-90
  • Applications tips:
Description: A polyclonal antibody for detection of GPR12 from Human. This GPR12 antibody is for WB, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the N-terminal region of human GPR12 at AA rangle: 10-90

GPR12 siRNA

20-abx902268
  • EUR 551.00
  • EUR 732.00
  • 0
  • 1
  • Shipped within 5-10 working days.

GPR12 siRNA

20-abx918427
  • EUR 551.00
  • EUR 732.00
  • 0
  • 1
  • Shipped within 5-10 working days.

GPR12 siRNA

20-abx918428
  • EUR 551.00
  • EUR 732.00
  • 0
  • 1
  • Shipped within 5-10 working days.

GPR12 Antibody, HRP conjugated

1-CSB-PA009742LB01HU
  • EUR 317.00
  • EUR 335.00
  • 0
  • 1
  • Form: Liquid
  • Buffer: Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against GPR12. Recognizes GPR12 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA

GPR12 Antibody, FITC conjugated

1-CSB-PA009742LC01HU
  • EUR 317.00
  • EUR 335.00
  • 0
  • 1
  • Form: Liquid
  • Buffer: Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against GPR12. Recognizes GPR12 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA

GPR12 Antibody, Biotin conjugated

1-CSB-PA009742LD01HU
  • EUR 317.00
  • EUR 335.00
  • 0
  • 1
  • Form: Liquid
  • Buffer: Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against GPR12. Recognizes GPR12 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA

GPR12 Blocking Peptide

20-abx062679
  • EUR 272.00
  • EUR 411.00
  • 0
  • 1
  • Shipped within 5-10 working days.

GPR12 Blocking Peptide

DF2721-BP 1mg
EUR 195.00

Polyclonal GPR12 Antibody (Extracellular Domain)

APR16480G 0.05mg
EUR 484.00
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human GPR12 (Extracellular Domain). This antibody is tested and proven to work in the following applications:

Polyclonal GPR12 Antibody (N-Terminus)

APR16481G 0.05mg
EUR 484.00
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human GPR12 (N-Terminus). This antibody is tested and proven to work in the following applications:

Polyclonal Goat anti-GST α-form

GST-ANTI-1 50 uL
EUR 280.00

Polyclonal Goat anti-GST μ-form

GST-ANTI-2 50 uL
EUR 280.00

Polyclonal Goat anti-GST p-form

GST-ANTI-3 50 uL
EUR 280.00

Rat GPR12 shRNA Plasmid

20-abx986567
  • EUR 801.00
  • EUR 1121.00
  • 0
  • 1
  • Shipped within 15-20 working days.

GPR12 ELISA KIT|Human

EF005024 96 Tests
EUR 689.00

Mouse GPR12 shRNA Plasmid

20-abx970621
  • EUR 801.00
  • EUR 1121.00
  • 0
  • 1
  • Shipped within 15-20 working days.

Human GPR12 shRNA Plasmid

20-abx951900
  • EUR 801.00
  • EUR 1121.00
  • 0
  • 1
  • Shipped within 15-20 working days.

GPR12 Recombinant Protein (Human)

RP061035 100 ug Ask for price

GPR12 Recombinant Protein (Rat)

RP203285 100 ug Ask for price

GPR12 Recombinant Protein (Rat)

RP203288 100 ug Ask for price

GPR12 Recombinant Protein (Mouse)

RP139421 100 ug Ask for price

GPR12 Recombinant Protein (Mouse)

RP139424 100 ug Ask for price

Polyclonal GPR12 Antibody - C-terminal region

APR16482G 0.05mg
EUR 528.00
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human GPR12 - C-terminal region. This antibody is tested and proven to work in the following applications:

G Protein Coupled Receptor 12 (GPR12) Antibody

20-abx147559
  • EUR 425.00
  • EUR 342.00
  • 0
  • 1
  • Shipped within 5-10 working days.

G Protein Coupled Receptor 12 (GPR12) Antibody

20-abx009208
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 0
  • 1
  • 2
  • Shipped within 5-10 working days.

G Protein Coupled Receptor 12 (GPR12) Antibody

20-abx324375
  • EUR 314.00
  • EUR 244.00
  • 0
  • 1
  • Shipped within 5-10 working days.

G Protein Coupled Receptor 12 (GPR12) Antibody

20-abx338465
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 0
  • 1
  • 2
  • 3
  • 4
  • Shipped within 5-10 working days.

Gpr12 ORF Vector (Rat) (pORF)

ORF067763 1.0 ug DNA
EUR 506.00

Gpr12 ORF Vector (Rat) (pORF)

ORF067764 1.0 ug DNA
EUR 506.00

Gpr12 ORF Vector (Mouse) (pORF)

ORF046475 1.0 ug DNA
EUR 506.00

Gpr12 ORF Vector (Mouse) (pORF)

ORF046476 1.0 ug DNA
EUR 506.00

GPR12 ORF Vector (Human) (pORF)

ORF020346 1.0 ug DNA
EUR 405.00

G Protein Coupled Receptor 12 (GPR12) Antibody (HRP)

20-abx335850
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 0
  • 1
  • 2
  • 3
  • 4
  • Shipped within 5-10 working days.

G Protein Coupled Receptor 12 (GPR12) Antibody (FITC)

20-abx335851
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 0
  • 1
  • 2
  • 3
  • 4
  • Shipped within 5-10 working days.

G Protein Coupled Receptor 12 (GPR12) Antibody (Biotin)

20-abx335852
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 0
  • 1
  • 2
  • 3
  • 4
  • Shipped within 5-10 working days.

GPR12 sgRNA CRISPR Lentivector set (Human)

K0889201 3 x 1.0 ug
EUR 339.00

Gpr12 sgRNA CRISPR Lentivector set (Mouse)

K3382801 3 x 1.0 ug
EUR 339.00

Gpr12 sgRNA CRISPR Lentivector set (Rat)

K7010601 3 x 1.0 ug
EUR 339.00

GPR12 sgRNA CRISPR Lentivector (Human) (Target 1)

K0889202 1.0 ug DNA
EUR 154.00

GPR12 sgRNA CRISPR Lentivector (Human) (Target 2)

K0889203 1.0 ug DNA
EUR 154.00

GPR12 sgRNA CRISPR Lentivector (Human) (Target 3)

K0889204 1.0 ug DNA
EUR 154.00

Gpr12 sgRNA CRISPR Lentivector (Mouse) (Target 1)

K3382802 1.0 ug DNA
EUR 154.00

Gpr12 sgRNA CRISPR Lentivector (Mouse) (Target 2)

K3382803 1.0 ug DNA
EUR 154.00

Gpr12 sgRNA CRISPR Lentivector (Mouse) (Target 3)

K3382804 1.0 ug DNA
EUR 154.00

Gpr12 sgRNA CRISPR Lentivector (Rat) (Target 1)

K7010602 1.0 ug DNA
EUR 154.00

Gpr12 sgRNA CRISPR Lentivector (Rat) (Target 2)

K7010603 1.0 ug DNA
EUR 154.00

Gpr12 sgRNA CRISPR Lentivector (Rat) (Target 3)

K7010604 1.0 ug DNA
EUR 154.00

GPR12 Protein Vector (Human) (pPB-C-His)

PV081381 500 ng
EUR 552.00

GPR12 Protein Vector (Human) (pPB-N-His)

PV081382 500 ng
EUR 552.00

GPR12 Protein Vector (Human) (pPM-C-HA)

PV081383 500 ng
EUR 552.00

GPR12 Protein Vector (Human) (pPM-C-His)

PV081384 500 ng
EUR 552.00

GPR12 Protein Vector (Mouse) (pPB-C-His)

PV185898 500 ng
EUR 603.00

GPR12 Protein Vector (Mouse) (pPB-N-His)

PV185899 500 ng
EUR 603.00

GPR12 Protein Vector (Mouse) (pPM-C-HA)

PV185900 500 ng
EUR 603.00

GPR12 Protein Vector (Mouse) (pPM-C-His)

PV185901 500 ng
EUR 603.00

GPR12 Protein Vector (Mouse) (pPB-C-His)

PV185902 500 ng
EUR 603.00

GPR12 Protein Vector (Mouse) (pPB-N-His)

PV185903 500 ng
EUR 603.00

GPR12 Protein Vector (Mouse) (pPM-C-HA)

PV185904 500 ng
EUR 603.00

GPR12 Protein Vector (Mouse) (pPM-C-His)

PV185905 500 ng
EUR 603.00

GPR12 Protein Vector (Rat) (pPB-C-His)

PV271050 500 ng
EUR 603.00

GPR12 Protein Vector (Rat) (pPB-N-His)

PV271051 500 ng
EUR 603.00

GPR12 Protein Vector (Rat) (pPM-C-HA)

PV271052 500 ng
EUR 603.00

GPR12 Protein Vector (Rat) (pPM-C-His)

PV271053 500 ng
EUR 603.00

GPR12 Protein Vector (Rat) (pPB-C-His)

PV271054 500 ng
EUR 603.00

GPR12 Protein Vector (Rat) (pPB-N-His)

PV271055 500 ng
EUR 603.00

GPR12 Protein Vector (Rat) (pPM-C-HA)

PV271056 500 ng
EUR 603.00

GPR12 Protein Vector (Rat) (pPM-C-His)

PV271057 500 ng
EUR 603.00

Gpr12 3'UTR Luciferase Stable Cell Line

TU205326 1.0 ml Ask for price

Gpr12 3'UTR GFP Stable Cell Line

TU158969 1.0 ml Ask for price

GPR12 3'UTR Luciferase Stable Cell Line

TU009144 1.0 ml
EUR 1394.00

Gpr12 3'UTR Luciferase Stable Cell Line

TU108969 1.0 ml Ask for price

GPR12 3'UTR GFP Stable Cell Line

TU059144 1.0 ml
EUR 1394.00

Gpr12 3'UTR GFP Stable Cell Line

TU255326 1.0 ml Ask for price

GPR12 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV)

LV626605 1.0 ug DNA
EUR 682.00

GPR12 Lentiviral Vector (Rat) (UbC) (pLenti-GIII-UbC)

LV626609 1.0 ug DNA
EUR 682.00

GPR12 Lentiviral Vector (Rat) (EF1a) (pLenti-GIII-EF1a)

LV626610 1.0 ug DNA
EUR 682.00

GPR12 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV)

LV652531 1.0 ug DNA
EUR 682.00

GPR12 Lentiviral Vector (Rat) (UbC) (pLenti-GIII-UbC)

LV652535 1.0 ug DNA
EUR 682.00

GPR12 Lentiviral Vector (Rat) (EF1a) (pLenti-GIII-EF1a)

LV652536 1.0 ug DNA
EUR 682.00

Mouse G- protein coupled receptor 12, Gpr12 ELISA KIT

ELI-09763m 96 Tests
EUR 865.00

Human G- protein coupled receptor 12, GPR12 ELISA KIT

ELI-19453h 96 Tests
EUR 824.00

Human G Protein Coupled Receptor 12 (GPR12) ELISA Kit

abx384941-96tests 96 tests
EUR 911.00
  • Shipped within 5-12 working days.

Mouse G Protein Coupled Receptor 12 (GPR12) ELISA Kit

abx389367-96tests 96 tests
EUR 911.00
  • Shipped within 5-12 working days.

Rat G Protein Coupled Receptor 12 (GPR12) ELISA Kit

abx391367-96tests 96 tests
EUR 911.00
  • Shipped within 5-12 working days.

GPR12 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Human)

K0889205 3 x 1.0 ug
EUR 376.00

Gpr12 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Mouse)

K3382805 3 x 1.0 ug
EUR 376.00

Gpr12 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Rat)

K7010605 3 x 1.0 ug
EUR 376.00

Gpr12 ELISA Kit| Rat G-protein coupled receptor 12 ELISA Kit

EF018723 96 Tests
EUR 689.00

Gpr12 ELISA Kit| Mouse G-protein coupled receptor 12 ELISA Kit

EF015000 96 Tests
EUR 689.00

GPR12 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 1)

K0889206 1.0 ug DNA
EUR 167.00

GPR12 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 2)

K0889207 1.0 ug DNA
EUR 167.00

GPR12 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 3)

K0889208 1.0 ug DNA
EUR 167.00

Gpr12 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 1)

K3382806 1.0 ug DNA
EUR 167.00

Gpr12 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 2)

K3382807 1.0 ug DNA
EUR 167.00

Gpr12 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 3)

K3382808 1.0 ug DNA
EUR 167.00
Enhancing dimerization did not automatically lead to better vector RNA packaging, suggesting that, for vector functionality, sufficient flexibility of the RNA to adopt different conformations is more important than the dimerization capacity. Our novel competitive qPCR assay enables a more stringent analysis of RNA packaging efficiency, allowing a much more precise understanding of the links between RNA structure, packaging, and infectious titers that will be invaluable for future vector development.

At what times during infection is SARS-CoV-2 detectable and no longer detectable using RT-PCR-based tests? A systematic review of individual participant data

 

Background: Tests for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viral ribonucleic acid (RNA) using reverse transcription polymerase chain reaction (RT-PCR) are pivotal to detecting current coronavirus disease (COVID-19) and duration of detectable virus indicating potential for infectivity.
Methods: We conducted an individual participant data (IPD) systematic review of longitudinal studies of RT-PCR test results in symptomatic SARS-CoV-2. We searched PubMed, LitCOVID, medRxiv, and COVID-19 Living Evidence databases. We assessed risk of bias using a QUADAS-2 adaptation. Outcomes were the percentage of positive test results by time and the duration of detectable virus, by anatomical sampling sites.
Results: Of 5078 studies screened, we included 32 studies with 1023 SARS-CoV-2 infected participants and 1619 test results, from – 6 to 66 days post-symptom onset and hospitalisation. The highest percentage virus detection was from nasopharyngeal sampling between 0 and 4 days post-symptom onset at 89% (95% confidence interval (CI) 83 to 93) dropping to 54% (95% CI 47 to 61) after 10 to 14 days. On average, duration of detectable virus was longer with lower respiratory tract (LRT) sampling than upper respiratory tract (URT). Duration of faecal and respiratory tract virus detection varied greatly within individual participants. In some participants, virus was still detectable at 46 days post-symptom onset.
Conclusions: RT-PCR misses detection of people with SARS-CoV-2 infection; early sampling minimises false negative diagnoses. Beyond 10 days post-symptom onset, lower RT or faecal testing may be preferred sampling sites. The included studies are open to substantial risk of bias, so the positivity rates are probably overestimated.

A real-time PCR assay to detect predation by spiny dogfish on Atlantic cod in the western North Atlantic Ocean

Conventional observations show spiny dogfish (Squalus acanthius Linnaeus) rarely eat Atlantic cod (Gadus morhua Linnaeus; 0.02% of stomachs) in the northwestern Atlantic Ocean. Critics express concern that digestion may limit species-level prey identification, and with recovery from overfishing, dogfish populations may be suppressing cod by competition or predation.
This study applied a real-time PCR TaqMan assay to identify cod in dogfish stomachs collected by cooperating fishing boats during normal trawling operations (May 2014-May 2015; Gulf of Maine, Georges Bank). Conventional methods observed 51 different prey taxa and nearly 1,600 individual prey items, but no cod were observed. Cod DNA was detected in 31 (10.5%) of the dogfish stomachs, with a higher percentage of these from the homogenate of amorphous, well-digested prey and stomach fluids (20 stomachs or 65%) than from discrete animal tissues (11 stomachs or 35%). Re-examination of photographs of these 11 tissue samples revealed one whole, partially digested fish that could be recognized in hindsight as cod.
Cod DNA was observed in dogfish stomachs year round: in January (1 of 1 trip), February (1 of 1), May (1 of 3), June (0 of 1), July (3 of 4), August (1 of 2), and October (3 of 3). Although these data suggest higher interaction rates between dogfish and cod than previously observed, addressing the population consequences of this predator-prey relationship requires a robust sampling design, estimates of digestion rates by dogfish to account for complete degradation of DNA sequences, and consideration for dogfish scavenging during fishing operations.

Multiple PCR assay based on the cigR gene for detection of Salmonella spp. and Salmonella Pullorum/Gallinarum identification

Salmonella spp. are important zoonotic pathogens that are responsible for severe diseases in both animals and humans. Salmonella enterica subsp. enterica serovar Gallinarum biovar Gallinarum (S. Gallinarum) and biovar Pullorum (S. Pullorum) are typical infectious pathogens detected in the chicken industry that have caused great economic losses. To facilitate their detection and prevent contamination, we developed a rapid multiple PCR method, which can simultaneously detect Salmonella spp. and further identify the biovars S. Pullorum/Gallinarum. This PCR detection method is based on the cigR gene, which is conserved among Salmonella spp. but has a 42-bp deletion in S. Pullorum/Gallinarum. The specificity and sensitivity of the PCR assay was evaluated with 41 different strains: 34 Salmonella strains, including 5 S. Pullorum/Gallinarum strains, and 7 non-Salmonella strains.
The lower limit of detection was 8.15 pg of S. Pullorum (S06004) genomic DNA and 20 cfu in PCR, which shows a great sensitivity. In addition, this method was applied to detect or identify Salmonella from processing chicken liver and egg samples, and the results corresponded to those obtained from serotype analysis using the conventional slide agglutination test. Overall, the new cigR-based PCR assay is efficient and practical for Salmonella detection and S. Pullorum/Gallinarum identification and will greatly reduce the workload of epidemiologic investigation.
Virus Zika PCR kit
PCR-H434-96R 100T
EUR 669.40
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of Virus Zika
MDV Real-Time PCR Kit
PD65-06 96t
EUR 1553.60
Description: Please check the datasheet of MDV Real-Time PCR Kit before using the test.
ALV Real-Time PCR Kit
PD65-15 96t
EUR 1724.40
Description: Please check the datasheet of ALV Real-Time PCR Kit before using the test.
hsa-mir-521 Real-time RT-PCR Detection Kit
20-abx097600
  • EUR 551.00
  • EUR 787.00
  • EUR 398.00
  • 0
  • 1
  • 2
  • Shipped within 3 weeks.
ELISA kit for Human Zika Virus (ZIKV)  Kit
KTE62982-48T 48T
EUR 354.00
Description: Quantitative sandwich ELISA for measuring Human Zika Virus (ZIKV)  Kit in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
ELISA kit for Human Zika Virus (ZIKV)  Kit
KTE62982-5platesof96wells 5 plates of 96 wells
EUR 2252.00
Description: Quantitative sandwich ELISA for measuring Human Zika Virus (ZIKV)  Kit in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
ELISA kit for Human Zika Virus (ZIKV)  Kit
KTE62982-96T 96T
EUR 572.00
Description: Quantitative sandwich ELISA for measuring Human Zika Virus (ZIKV)  Kit in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
Novel Coronavirus COVID-19 (2019-nCoV) Real Time RT-PCR Kit
RR-0478-02 25 tests/kit
EUR 991.00
  • For use with PE5700, MJ-Opticon & other single color systems, ABI7000, ABI7300, ABI7500, ABI7900, ABI StepOne, StepOne plus, MJ-Opticon2, MJ-chromo4, MX3000P, MX3005P, Smart Cycler II, Rotor-Gene 6000, LightCycler 480, CFX 96, Life 96, Slan 96, iCycl
  • Show more
Description: Novel Coronavirus (2019-nCoV) Real Time RT-PCR Kit is used for the qualitative detection of a novel coronavirus, which was identified in 2019 at Wuhan City, Hubei Province, China, in upper respiratory tract specimens (nasopharyngeal extracts, deep cough sputum, etc.) and lower respiratory tract specimens (alveoli irrigation fluid, etc.) by real time PCR systems.
hsa-mir-521 Real-Time RT-PCR Detection and U6 Calibration Kit
20-abx097601
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 0
  • 1
  • 2
  • Shipped within 3 weeks.
0,1ML THIN WALL TUBES 8 PER STRIP. CLEAR & REAL TIME STRIP CAPS
PCR-0108-LP-RT-C 125/pk
EUR 709.00
Description: PCR Plates & Tubes; PCR Strip Tubes and Strip Caps - Axygen
0,1ML THIN WALL TUBES 8 PER STRIP. WHITE & REAL TIME STRIP CAPS
PCR-0108-LP-RT-W 125/pk
EUR 770.00
Description: PCR Plates & Tubes; PCR Strip Tubes and Strip Caps - Axygen
Virus Zika One-Step PCR kit
Oneq-H434-100R 100T
EUR 933.00
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Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Virus Zika.
Virus Zika One-Step PCR kit
Oneq-H434-150R 150T
EUR 1060.60
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Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Virus Zika.
Virus Zika One-Step PCR kit
Oneq-H434-50R 50T
EUR 751.75
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Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Virus Zika.
Dengue virus RT PCR kit
RTq-H451-100R 100T
EUR 776.40
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Description: A Real-Time PCR kit for detection of Dengue virus .
Dengue virus RT PCR kit
RTq-H451-150R 150T
EUR 877.90
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Description: A Real-Time PCR kit for detection of Dengue virus .
Dengue virus RT PCR kit
RTq-H451-50R 50T
EUR 631.40
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Description: A Real-Time PCR kit for detection of Dengue virus .
Rubella virus RT PCR kit
RTq-H481-100R 100T
EUR 776.40
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Description: A Real-Time PCR kit for detection of Rubella virus .
Rubella virus RT PCR kit
RTq-H481-150R 150T
EUR 877.90
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Description: A Real-Time PCR kit for detection of Rubella virus .
Rubella virus RT PCR kit
RTq-H481-50R 50T
EUR 631.40
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Description: A Real-Time PCR kit for detection of Rubella virus .
Chikungunya virus RT PCR kit
RTq-H525-100R 100T
EUR 776.40
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Description: A Real-Time PCR kit for detection of Chikungunya virus .
Chikungunya virus RT PCR kit
RTq-H525-150R 150T
EUR 877.90
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Description: A Real-Time PCR kit for detection of Chikungunya virus .
Chikungunya virus RT PCR kit
RTq-H525-50R 50T
EUR 631.40
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Description: A Real-Time PCR kit for detection of Chikungunya virus .
Measles Virus RT PCR kit
RTq-H639-100R 100T
EUR 776.40
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Description: A Real-Time PCR kit for detection of Measles Virus .
Measles Virus RT PCR kit
RTq-H639-150R 150T
EUR 877.90
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Description: A Real-Time PCR kit for detection of Measles Virus .
Measles Virus RT PCR kit
RTq-H639-50R 50T
EUR 631.40
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Description: A Real-Time PCR kit for detection of Measles Virus .
Mumps Virus RT PCR kit
RTq-H640-100R 100T
EUR 776.40
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Description: A Real-Time PCR kit for detection of Mumps Virus .
Mumps Virus RT PCR kit
RTq-H640-150R 150T
EUR 877.90
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Description: A Real-Time PCR kit for detection of Mumps Virus .
Mumps Virus RT PCR kit
RTq-H640-50R 50T
EUR 631.40
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Description: A Real-Time PCR kit for detection of Mumps Virus .
Uukuniemi virus RT PCR kit
RTq-V056-100R 100T
EUR 776.40
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Description: A Real-Time PCR kit for detection of Uukuniemi virus .
Uukuniemi virus RT PCR kit
RTq-V056-150R 150T
EUR 701.00
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Description: A Real-Time PCR kit for detection of Uukuniemi virus .
Uukuniemi virus RT PCR kit
RTq-V056-50R 50T
EUR 631.40
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Description: A Real-Time PCR kit for detection of Uukuniemi virus .
Reticuloendotheliosis virus RT PCR kit
RTq-V061-100R 100T
EUR 776.40
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Description: A Real-Time PCR kit for detection of Reticuloendotheliosis virus .
Reticuloendotheliosis virus RT PCR kit
RTq-V061-150R 150T
EUR 877.90
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Description: A Real-Time PCR kit for detection of Reticuloendotheliosis virus .
Reticuloendotheliosis virus RT PCR kit
RTq-V061-50R 50T
EUR 631.40
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Description: A Real-Time PCR kit for detection of Reticuloendotheliosis virus .
Distemper virus RT PCR kit
RTq-V111-100R 100T
EUR 776.40
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Description: A Real-Time PCR kit for detection of Distemper virus .
Distemper virus RT PCR kit
RTq-V111-150R 150T
EUR 877.90
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Description: A Real-Time PCR kit for detection of Distemper virus .
Distemper virus RT PCR kit
RTq-V111-50R 50T
EUR 631.40
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Description: A Real-Time PCR kit for detection of Distemper virus .
Pseudorabies Virus RT PCR kit
RTq-V123-100D 100T
EUR 628.50
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Description: A Real-Time PCR kit for detection of Pseudorabies Virus .
Pseudorabies Virus RT PCR kit
RTq-V123-150D 150T
EUR 701.00
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Description: A Real-Time PCR kit for detection of Pseudorabies Virus .
Pseudorabies Virus RT PCR kit
RTq-V123-50D 50T
EUR 532.80
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Description: A Real-Time PCR kit for detection of Pseudorabies Virus .
Cowpox virus RT PCR kit
RTq-V173-100D 100T
EUR 628.50
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Description: A Real-Time PCR kit for detection of Cowpox virus .
Cowpox virus RT PCR kit
RTq-V173-150D 150T
EUR 701.00
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Description: A Real-Time PCR kit for detection of Cowpox virus .
Cowpox virus RT PCR kit
RTq-V173-50D 50T
EUR 532.80
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Description: A Real-Time PCR kit for detection of Cowpox virus .
Akabane virus RT PCR kit
RTq-V267-100R 100T
EUR 776.40
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Description: A Real-Time PCR kit for detection of Akabane virus .
Akabane virus RT PCR kit
RTq-V267-150R 150T
EUR 877.90
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Description: A Real-Time PCR kit for detection of Akabane virus .
Akabane virus RT PCR kit
RTq-V267-50R 50T
EUR 631.40
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Description: A Real-Time PCR kit for detection of Akabane virus .
Irido virus RT PCR kit
RTq-V288-100D 100T
EUR 628.50
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Description: A Real-Time PCR kit for detection of Irido virus .
Irido virus RT PCR kit
RTq-V288-150D 150T
EUR 701.00
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Description: A Real-Time PCR kit for detection of Irido virus .
Irido virus RT PCR kit
RTq-V288-50D 50T
EUR 532.80
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Description: A Real-Time PCR kit for detection of Irido virus .
Mourilyan virus RT PCR kit
RTq-V291-100R 100T
EUR 776.40
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Description: A Real-Time PCR kit for detection of Mourilyan virus .
Mourilyan virus RT PCR kit
RTq-V291-150R 150T
EUR 877.90
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Description: A Real-Time PCR kit for detection of Mourilyan virus .
Mourilyan virus RT PCR kit
RTq-V291-50R 50T
EUR 631.40
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Description: A Real-Time PCR kit for detection of Mourilyan virus .
Schmallenberg virus RT PCR kit
RTq-V319-100R 100T
EUR 776.40
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Description: A Real-Time PCR kit for detection of Schmallenberg virus .
Schmallenberg virus RT PCR kit
RTq-V319-150R 150T
EUR 877.90
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Description: A Real-Time PCR kit for detection of Schmallenberg virus .
Schmallenberg virus RT PCR kit
RTq-V319-50R 50T
EUR 631.40
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Description: A Real-Time PCR kit for detection of Schmallenberg virus .
Ectromelia virus RT PCR kit
RTq-V330-100D 100T
EUR 628.50
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Description: A Real-Time PCR kit for detection of Ectromelia virus .
Ectromelia virus RT PCR kit
RTq-V330-150D 150T
EUR 701.00
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Description: A Real-Time PCR kit for detection of Ectromelia virus .
Ectromelia virus RT PCR kit
RTq-V330-50D 50T
EUR 532.80
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Description: A Real-Time PCR kit for detection of Ectromelia virus .
K Virus RT PCR kit
RTq-V365-100D 100T
EUR 628.50
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Description: A Real-Time PCR kit for detection of K Virus .
K Virus RT PCR kit
RTq-V365-150D 150T
EUR 701.00
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Description: A Real-Time PCR kit for detection of K Virus .
K Virus RT PCR kit
RTq-V365-50D 50T
EUR 532.80
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Description: A Real-Time PCR kit for detection of K Virus .
Sialodacryoadenitis virus RT PCR kit
RTq-V370-100R 100T
EUR 776.40
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Description: A Real-Time PCR kit for detection of Sialodacryoadenitis virus .
Sialodacryoadenitis virus RT PCR kit
RTq-V370-150R 150T
EUR 877.90
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Description: A Real-Time PCR kit for detection of Sialodacryoadenitis virus .
Sialodacryoadenitis virus RT PCR kit
RTq-V370-50R 50T
EUR 631.40
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Description: A Real-Time PCR kit for detection of Sialodacryoadenitis virus .
Sendai virus RT PCR kit
RTq-V374-100R 100T
EUR 776.40
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Description: A Real-Time PCR kit for detection of Sendai virus .
Sendai virus RT PCR kit
RTq-V374-150R 150T
EUR 877.90
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Description: A Real-Time PCR kit for detection of Sendai virus .
Sendai virus RT PCR kit
RTq-V374-50R 50T
EUR 631.40
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Description: A Real-Time PCR kit for detection of Sendai virus .
Junin Virus RT PCR kit
RTq-V388-100R 100T
EUR 776.40
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Description: A Real-Time PCR kit for detection of Junin Virus .
Junin Virus RT PCR kit
RTq-V388-150R 150T
EUR 877.90
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Description: A Real-Time PCR kit for detection of Junin Virus .
Junin Virus RT PCR kit
RTq-V388-50R 50T
EUR 631.40
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Description: A Real-Time PCR kit for detection of Junin Virus .
Machupo Virus RT PCR kit
RTq-V389-100R 100T
EUR 776.40
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Description: A Real-Time PCR kit for detection of Machupo Virus .
Machupo Virus RT PCR kit
RTq-V389-150R 150T
EUR 877.90
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Description: A Real-Time PCR kit for detection of Machupo Virus .
Machupo Virus RT PCR kit
RTq-V389-50R 50T
EUR 631.40
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Description: A Real-Time PCR kit for detection of Machupo Virus .
Lassa virus RT PCR kit
RTq-V390-100R 100T
EUR 776.40
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Description: A Real-Time PCR kit for detection of Lassa virus .
Lassa virus RT PCR kit
RTq-V390-150R 150T
EUR 877.90
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Description: A Real-Time PCR kit for detection of Lassa virus .
Lassa virus RT PCR kit
RTq-V390-50R 50T
EUR 631.40
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Description: A Real-Time PCR kit for detection of Lassa virus .
Guanarito virus RT PCR kit
RTq-V391-100R 100T
EUR 776.40
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Description: A Real-Time PCR kit for detection of Guanarito virus .
Guanarito virus RT PCR kit
RTq-V391-150R 150T
EUR 877.90
  • Contact us in order to know the reactivity of the kit.
Description: A Real-Time PCR kit for detection of Guanarito virus .
Guanarito virus RT PCR kit
RTq-V391-50R 50T
EUR 631.40
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Description: A Real-Time PCR kit for detection of Guanarito virus .
Sabia Virus RT PCR kit
RTq-V392-100R 100T
EUR 776.40
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Description: A Real-Time PCR kit for detection of Sabia Virus .
Sabia Virus RT PCR kit
RTq-V392-150R 150T
EUR 877.90
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Description: A Real-Time PCR kit for detection of Sabia Virus .
Sabia Virus RT PCR kit
RTq-V392-50R 50T
EUR 631.40
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Description: A Real-Time PCR kit for detection of Sabia Virus .
Ecephalomyocarditis virus RT PCR kit
RTq-V394-100R 100T
EUR 776.40
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Description: A Real-Time PCR kit for detection of Ecephalomyocarditis virus .
Ecephalomyocarditis virus RT PCR kit
RTq-V394-150R 150T
EUR 877.90
  • Contact us in order to know the reactivity of the kit.
Description: A Real-Time PCR kit for detection of Ecephalomyocarditis virus .
Ecephalomyocarditis virus RT PCR kit
RTq-V394-50R 50T
EUR 631.40
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Description: A Real-Time PCR kit for detection of Ecephalomyocarditis virus .
Visna virus RT PCR kit
RTq-V634-100R 100T
EUR 776.40
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Description: A Real-Time PCR kit for detection of Visna virus .
Visna virus RT PCR kit
RTq-V634-150R 150T
EUR 877.90
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Description: A Real-Time PCR kit for detection of Visna virus .
Visna virus RT PCR kit
RTq-V634-50R 50T
EUR 631.40
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Description: A Real-Time PCR kit for detection of Visna virus .
Swinepox virus RT PCR kit
RTq-V727-100D 100T
EUR 628.50
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Description: A Real-Time PCR kit for detection of Swinepox virus .