Prospective case-control study of enterovirus detection differences in children’s cerebrospinal fluid between multiplex PCR and real-time RT-PCR assay
Background: Viral encephalitis is common in childhood. It is an acute brain parenchymal inflammation caused by a variety of viral infection, and enterovirus accounts for the majority. Due to atypical clinical manifestations, pathogenic testing is important for assisting clinical diagnosis. The purpose of this study was to evaluate the performance of the multiplex PCR assay compared with quantitative real-time PCR for enterovirus detection.
Methods: A prospective case-control study was performed involving 103 pediatric patients suspected for viral encephalitis and cerebrospinal fluid (CSF) samples were collected and tested for 9 pathogens using multiplex PCR assay during April to November in 2018. In parallel, an aliquot of samples was tested for enterovirus infection by real-time PCR assay.

mhealthinfo
Results: There were 85.4% children were confirmed as viral encephalitis on discharge, the remaining ones were diagnosed as other CNS diseases, such as epilepsy. The specificity of the two methods was the same as that of the clinical diagnosis, but the sensitivity and consistency with clinical diagnosis of multiplex PCR were both higher than the real-time PCR. Besides of enterovirus, multiplex PCR could also detect coinfection of enterovirus with Epstein-Barr virus and mumps virus.
Conclusion: Results of multiplex PCR method are more consistent with the clinical diagnosis and are superior to real-time PCR for detecting enterovirus in CSF.
Development of a Novel Competitive qRT-PCR Assay to Measure Relative Lentiviral Packaging Efficiency
Third-generation HIV-1-derived lentiviral vectors are successfully used as therapeutic agents in various clinical applications. To further promote their use, we attempted to enhance vector infectivity by targeting the dimerization and packaging properties of the RNA transfer vector based on the premise that these two processes are tightly linked.
We rationally designed mutant vectors to favor the dimeric conformation, potentially enhancing genome packaging. Initial assessments using standard assays generated outputs of variable reproducibility, sometimes with conflicting results. Therefore, we developed a novel competitive qRT-PCR assay in a co-transfection setting to measure the relative packaging efficiencies of wild-type and mutant transfer vectors. Here we report the effect of the dimerization-stabilizing mutations on infectious and physical titers of lentiviral vectors together with their packaging efficiency, measured using our novel assay.
GPR12 Polyclonal Antibody |
ABP54586-003ml |
Abbkine |
0.03ml |
EUR 189.6 |
|
Description: A polyclonal antibody for detection of GPR12 from Human. This GPR12 antibody is for WB, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the N-terminal region of human GPR12 at AA rangle: 10-90 |
GPR12 Polyclonal Antibody |
ABP54586-01ml |
Abbkine |
0.1ml |
EUR 346.8 |
|
Description: A polyclonal antibody for detection of GPR12 from Human. This GPR12 antibody is for WB, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the N-terminal region of human GPR12 at AA rangle: 10-90 |
GPR12 Polyclonal Antibody |
ABP54586-02ml |
Abbkine |
0.2ml |
EUR 496.8 |
|
Description: A polyclonal antibody for detection of GPR12 from Human. This GPR12 antibody is for WB, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the N-terminal region of human GPR12 at AA rangle: 10-90 |
GPR12 Polyclonal Antibody |
ES5585-100ul |
ELK Biotech |
100ul |
EUR 334.8 |
Description: A Rabbit Polyclonal antibody against GPR12 from Human. This antibody is tested and validated for WB, ELISA, IF, WB, ELISA |
GPR12 Polyclonal Antibody |
ES5585-50ul |
ELK Biotech |
50ul |
EUR 248.4 |
Description: A Rabbit Polyclonal antibody against GPR12 from Human. This antibody is tested and validated for WB, ELISA, IF, WB, ELISA |
GPR12 Antibody, HRP conjugated |
1-CSB-PA009742LB01HU |
Cusabio |
|
|
|
Description: A polyclonal antibody against GPR12. Recognizes GPR12 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA |
GPR12 Antibody, FITC conjugated |
1-CSB-PA009742LC01HU |
Cusabio |
|
|
|
Description: A polyclonal antibody against GPR12. Recognizes GPR12 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA |
GPR12 Antibody, Biotin conjugated |
1-CSB-PA009742LD01HU |
Cusabio |
|
|
|
Description: A polyclonal antibody against GPR12. Recognizes GPR12 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA |
Polyclonal GPR12 Antibody (N-Terminus) |
APR16481G |
Leading Biology |
0.05mg |
EUR 580.8 |
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human GPR12 (N-Terminus). This antibody is tested and proven to work in the following applications: |
Polyclonal GPR12 Antibody (Extracellular Domain) |
APR16480G |
Leading Biology |
0.05mg |
EUR 580.8 |
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human GPR12 (Extracellular Domain). This antibody is tested and proven to work in the following applications: |
Polyclonal GPR12 Antibody - C-terminal region |
APR16482G |
Leading Biology |
0.05mg |
EUR 633.6 |
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human GPR12 - C-terminal region. This antibody is tested and proven to work in the following applications: |
G Protein Coupled Receptor 12 (GPR12) Antibody |
20-abx147559 |
Abbexa |
|
|
|
G Protein Coupled Receptor 12 (GPR12) Antibody |
20-abx324375 |
Abbexa |
|
|
|
G Protein Coupled Receptor 12 (GPR12) Antibody |
20-abx338465 |
Abbexa |
-
EUR 493.20
-
EUR 2214.00
-
EUR 718.80
-
EUR 218.40
-
EUR 360.00
|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
G Protein Coupled Receptor 12 (GPR12) Antibody |
20-abx009208 |
Abbexa |
-
EUR 360.00
-
EUR 526.80
-
EUR 226.80
|
|
|
G Protein Coupled Receptor 12 (GPR12) Antibody (HRP) |
20-abx335850 |
Abbexa |
-
EUR 493.20
-
EUR 2214.00
-
EUR 718.80
-
EUR 218.40
-
EUR 360.00
|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
G Protein Coupled Receptor 12 (GPR12) Antibody (FITC) |
20-abx335851 |
Abbexa |
-
EUR 493.20
-
EUR 2214.00
-
EUR 718.80
-
EUR 218.40
-
EUR 360.00
|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
G Protein Coupled Receptor 12 (GPR12) Antibody (Biotin) |
20-abx335852 |
Abbexa |
-
EUR 493.20
-
EUR 2214.00
-
EUR 718.80
-
EUR 218.40
-
EUR 360.00
|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
GPR12 siRNA |
20-abx918427 |
Abbexa |
|
|
|
GPR12 siRNA |
20-abx918428 |
Abbexa |
|
|
|
GPR12 siRNA |
20-abx902268 |
Abbexa |
|
|
|
Anti-GPR12 antibody |
STJ93328 |
St John's Laboratory |
200 µl |
EUR 236.4 |
Description: Rabbit polyclonal to GPR12. |
GPR12 Blocking Peptide |
20-abx062679 |
Abbexa |
|
|
|
GPR12 Blocking Peptide |
DF2721-BP |
Affbiotech |
1mg |
EUR 234 |
anti- GPR105 antibody |
FNab03588 |
FN Test |
100µg |
EUR 658.5 |
|
Description: Antibody raised against GPR105 |
anti- GPR107 antibody |
FNab03589 |
FN Test |
100µg |
EUR 702 |
|
Description: Antibody raised against GPR107 |
anti- GPR108 antibody |
FNab03590 |
FN Test |
100µg |
EUR 658.5 |
|
Description: Antibody raised against GPR108 |
anti- GPR116 antibody |
FNab03591 |
FN Test |
100µg |
EUR 658.5 |
|
Description: Antibody raised against GPR116 |
anti- GPR132 antibody |
FNab03593 |
FN Test |
100µg |
EUR 606.3 |
|
Description: Antibody raised against GPR132 |
anti- GPR137 antibody |
FNab03594 |
FN Test |
100µg |
EUR 606.3 |
|
Description: Antibody raised against GPR137 |
anti- GPR142 antibody |
FNab03597 |
FN Test |
100µg |
EUR 702 |
|
Description: Antibody raised against GPR142 |
anti- GPR161 antibody |
FNab03598 |
FN Test |
100µg |
EUR 606.3 |
|
Description: Antibody raised against GPR161 |
anti- GPR162 antibody |
FNab03599 |
FN Test |
100µg |
EUR 702 |
|
Description: Antibody raised against GPR162 |
anti- GPR177 antibody |
FNab03601 |
FN Test |
100µg |
EUR 658.5 |
|
Description: Antibody raised against GPR177 |
anti- GPR17 antibody |
FNab03600 |
FN Test |
100µg |
EUR 658.5 |
|
Description: Antibody raised against GPR17 |
anti- GPR22 antibody |
FNab03603 |
FN Test |
100µg |
EUR 606.3 |
|
Description: Antibody raised against GPR22 |
anti- GPR35 antibody |
FNab03605 |
FN Test |
100µg |
EUR 702 |
|
Description: Antibody raised against GPR35 |
anti- GPR50 antibody |
FNab03607 |
FN Test |
100µg |
EUR 658.5 |
|
Description: Antibody raised against GPR50 |
anti- GPR50 antibody |
FNab03608 |
FN Test |
100µg |
EUR 658.5 |
|
Description: Antibody raised against GPR50 |
anti- GPR81 antibody |
FNab03610 |
FN Test |
100µg |
EUR 702 |
|
Description: Antibody raised against GPR81 |
anti- GPR83 antibody |
FNab03611 |
FN Test |
100µg |
EUR 702 |
|
Description: Antibody raised against GPR83 |
anti- GPR137B antibody |
FNab03595 |
FN Test |
100µg |
EUR 702 |
|
Description: Antibody raised against GPR137B |
Anti-GPR12/Gpcr12 Antibody |
A10818 |
BosterBio |
100ul |
EUR 476.4 |
Description: Rabbit Polyclonal GPR12/Gpcr12 Antibody. Validated in IF and tested in Human. |
anti- GPR3 antibody |
FNab03604 |
FN Test |
100µg |
EUR 702 |
|
Description: Antibody raised against GPR3 |
Rat GPR12 shRNA Plasmid |
20-abx986567 |
Abbexa |
|
|
|
anti- GPR183/EBI2 antibody |
FNab03602 |
FN Test |
100µg |
EUR 658.5 |
|
Description: Antibody raised against GPR183/EBI2 |
Human GPR12 shRNA Plasmid |
20-abx951900 |
Abbexa |
|
|
|
Mouse GPR12 shRNA Plasmid |
20-abx970621 |
Abbexa |
|
|
|
Anti-OR51E1/Gpr164 Antibody |
A30874 |
BosterBio |
100ul |
EUR 476.4 |
Description: Rabbit Polyclonal OR51E1/Gpr164 Antibody. Validated in IF, WB and tested in Human. |
GPR12 Recombinant Protein (Rat) |
RP203285 |
ABM |
100 ug |
Ask for price |
GPR12 Recombinant Protein (Rat) |
RP203288 |
ABM |
100 ug |
Ask for price |
GPR12 Recombinant Protein (Mouse) |
RP139421 |
ABM |
100 ug |
Ask for price |
GPR12 Recombinant Protein (Mouse) |
RP139424 |
ABM |
100 ug |
Ask for price |
GPR12 Recombinant Protein (Human) |
RP061035 |
ABM |
100 ug |
Ask for price |
Anti-FFAR3/Gpr41 Antibody |
A02437 |
BosterBio |
100ul |
EUR 476.4 |
Description: Rabbit Polyclonal FFAR3/Gpr41 Antibody. Validated in IF and tested in Human, Mouse. |
anti- GPR101-Specific antibody |
FNab03587 |
FN Test |
100µg |
EUR 702 |
|
Description: Antibody raised against GPR101-Specific |
anti- GPR123-Specific antibody |
FNab03592 |
FN Test |
100µg |
EUR 702 |
|
Description: Antibody raised against GPR123-Specific |
anti- GPR139-Specific antibody |
FNab03596 |
FN Test |
100µg |
EUR 606.3 |
|
Description: Antibody raised against GPR139-Specific |
Anti-SUCNR1/Gpr91 Antibody |
A08485 |
BosterBio |
100ul |
EUR 476.4 |
Description: Rabbit Polyclonal SUCNR1/Gpr91 Antibody. Validated in WB and tested in Human, Mouse, Rat. |
anti- GPR58/TAAR2 antibody |
FNab03609 |
FN Test |
100µg |
EUR 658.5 |
|
Description: Antibody raised against GPR58/TAAR2 |
anti- GPR37/Pael-R antibody |
FNab03606 |
FN Test |
100µg |
EUR 606.3 |
|
Description: Antibody raised against GPR37/Pael-R |
Gpr12 ORF Vector (Rat) (pORF) |
ORF067763 |
ABM |
1.0 ug DNA |
EUR 607.2 |
Gpr12 ORF Vector (Rat) (pORF) |
ORF067764 |
ABM |
1.0 ug DNA |
EUR 607.2 |
GPR12 ORF Vector (Human) (pORF) |
ORF020346 |
ABM |
1.0 ug DNA |
EUR 486 |
Gpr12 ORF Vector (Mouse) (pORF) |
ORF046475 |
ABM |
1.0 ug DNA |
EUR 607.2 |
Gpr12 ORF Vector (Mouse) (pORF) |
ORF046476 |
ABM |
1.0 ug DNA |
EUR 607.2 |
Anti-GPCR GPR40/FFAR1 Antibody |
A01349-2 |
BosterBio |
100ug/vial |
EUR 352.8 |
Anti-LGR4/Gpr48 Monoclonal Antibody |
M02134 |
BosterBio |
100uL |
EUR 531.6 |
Description: Mouse Monoclonal LGR4/Gpr48 Antibody. Validated in IHC, WB and tested in Human. |
Polyclonal Goat Anti-TAS1R2 / GPR71 Antibody |
AMM05957G |
Leading Biology |
0.1 mg |
EUR 580.8 |
Description: A polyclonal antibody raised in Goat that recognizes and binds to Human Goat Anti-TAS1R2 / GPR71 . This antibody is tested and proven to work in the following applications: |
Gpr12 sgRNA CRISPR Lentivector set (Rat) |
K7010601 |
ABM |
3 x 1.0 ug |
EUR 406.8 |
Anti-GPCR GPR7/NPBWR1 Antibody |
A08247-1 |
BosterBio |
100ug/vial |
EUR 352.8 |
Anti-LGR5/GPR49 Rabbit Monoclonal Antibody |
M00239 |
BosterBio |
100ug/vial |
EUR 476.4 |
Description: Rabbit Monoclonal LGR5/GPR49 Antibody. Validated in Flow Cytometry, IP, WB and tested in Human, Mouse, Rat. |
GPR12 sgRNA CRISPR Lentivector set (Human) |
K0889201 |
ABM |
3 x 1.0 ug |
EUR 406.8 |
Gpr12 sgRNA CRISPR Lentivector set (Mouse) |
K3382801 |
ABM |
3 x 1.0 ug |
EUR 406.8 |
Anti Human GPR120 Monoclonal Antibody (Clone No. 2B6) |
KG138 |
Sceti |
each |
EUR 856.8 |
Description: The Anti Human GPR120 Monoclonal Antibody (Clone No. 2B6) is available in Europe and for worldwide shipping via Gentaur. |
Gpr12 3'UTR GFP Stable Cell Line |
TU158969 |
ABM |
1.0 ml |
Ask for price |
GPR12 3'UTR GFP Stable Cell Line |
TU059144 |
ABM |
1.0 ml |
EUR 1672.8 |
Gpr12 3'UTR GFP Stable Cell Line |
TU255326 |
ABM |
1.0 ml |
Ask for price |
Anti Mouse GPR40 Monoclonal Antibody (Clone No. G16) |
KG116 |
Sceti |
each |
EUR 919.2 |
Description: The Anti Mouse GPR40 Monoclonal Antibody (Clone No. G16) is available in Europe and for worldwide shipping via Gentaur. |
GPR12 Protein Vector (Rat) (pPM-C-HA) |
PV271052 |
ABM |
500 ng |
EUR 723.6 |
GPR12 Protein Vector (Rat) (pPM-C-HA) |
PV271056 |
ABM |
500 ng |
EUR 723.6 |
GPR12 Protein Vector (Rat) (pPB-C-His) |
PV271050 |
ABM |
500 ng |
EUR 723.6 |
GPR12 Protein Vector (Rat) (pPB-N-His) |
PV271051 |
ABM |
500 ng |
EUR 723.6 |
GPR12 Protein Vector (Rat) (pPM-C-His) |
PV271053 |
ABM |
500 ng |
EUR 723.6 |
GPR12 Protein Vector (Rat) (pPB-C-His) |
PV271054 |
ABM |
500 ng |
EUR 723.6 |
GPR12 Protein Vector (Rat) (pPB-N-His) |
PV271055 |
ABM |
500 ng |
EUR 723.6 |
GPR12 Protein Vector (Rat) (pPM-C-His) |
PV271057 |
ABM |
500 ng |
EUR 723.6 |
GPR12 Protein Vector (Mouse) (pPM-C-HA) |
PV185900 |
ABM |
500 ng |
EUR 723.6 |
GPR12 Protein Vector (Mouse) (pPM-C-HA) |
PV185904 |
ABM |
500 ng |
EUR 723.6 |
GPR12 Protein Vector (Human) (pPM-C-HA) |
PV081383 |
ABM |
500 ng |
EUR 662.4 |
GPR12 Protein Vector (Mouse) (pPB-C-His) |
PV185898 |
ABM |
500 ng |
EUR 723.6 |
GPR12 Protein Vector (Mouse) (pPB-N-His) |
PV185899 |
ABM |
500 ng |
EUR 723.6 |
GPR12 Protein Vector (Mouse) (pPM-C-His) |
PV185901 |
ABM |
500 ng |
EUR 723.6 |
GPR12 Protein Vector (Mouse) (pPB-C-His) |
PV185902 |
ABM |
500 ng |
EUR 723.6 |
GPR12 Protein Vector (Mouse) (pPB-N-His) |
PV185903 |
ABM |
500 ng |
EUR 723.6 |
GPR12 Protein Vector (Mouse) (pPM-C-His) |
PV185905 |
ABM |
500 ng |
EUR 723.6 |
GPR12 Protein Vector (Human) (pPB-C-His) |
PV081381 |
ABM |
500 ng |
EUR 662.4 |
GPR12 Protein Vector (Human) (pPB-N-His) |
PV081382 |
ABM |
500 ng |
EUR 662.4 |
GPR12 Protein Vector (Human) (pPM-C-His) |
PV081384 |
ABM |
500 ng |
EUR 662.4 |
Enhancing dimerization did not automatically lead to better vector RNA packaging, suggesting that, for vector functionality, sufficient flexibility of the RNA to adopt different conformations is more important than the dimerization capacity. Our novel competitive qPCR assay enables a more stringent analysis of RNA packaging efficiency, allowing a much more precise understanding of the links between RNA structure, packaging, and infectious titers that will be invaluable for future vector development.
At what times during infection is SARS-CoV-2 detectable and no longer detectable using RT-PCR-based tests? A systematic review of individual participant data
Background: Tests for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viral ribonucleic acid (RNA) using reverse transcription polymerase chain reaction (RT-PCR) are pivotal to detecting current coronavirus disease (COVID-19) and duration of detectable virus indicating potential for infectivity.
Methods: We conducted an individual participant data (IPD) systematic review of longitudinal studies of RT-PCR test results in symptomatic SARS-CoV-2. We searched PubMed, LitCOVID, medRxiv, and COVID-19 Living Evidence databases. We assessed risk of bias using a QUADAS-2 adaptation. Outcomes were the percentage of positive test results by time and the duration of detectable virus, by anatomical sampling sites.
Results: Of 5078 studies screened, we included 32 studies with 1023 SARS-CoV-2 infected participants and 1619 test results, from – 6 to 66 days post-symptom onset and hospitalisation. The highest percentage virus detection was from nasopharyngeal sampling between 0 and 4 days post-symptom onset at 89% (95% confidence interval (CI) 83 to 93) dropping to 54% (95% CI 47 to 61) after 10 to 14 days. On average, duration of detectable virus was longer with lower respiratory tract (LRT) sampling than upper respiratory tract (URT). Duration of faecal and respiratory tract virus detection varied greatly within individual participants. In some participants, virus was still detectable at 46 days post-symptom onset.
Conclusions: RT-PCR misses detection of people with SARS-CoV-2 infection; early sampling minimises false negative diagnoses. Beyond 10 days post-symptom onset, lower RT or faecal testing may be preferred sampling sites. The included studies are open to substantial risk of bias, so the positivity rates are probably overestimated.
A real-time PCR assay to detect predation by spiny dogfish on Atlantic cod in the western North Atlantic Ocean
Conventional observations show spiny dogfish (Squalus acanthius Linnaeus) rarely eat Atlantic cod (Gadus morhua Linnaeus; 0.02% of stomachs) in the northwestern Atlantic Ocean. Critics express concern that digestion may limit species-level prey identification, and with recovery from overfishing, dogfish populations may be suppressing cod by competition or predation.
This study applied a real-time PCR TaqMan assay to identify cod in dogfish stomachs collected by cooperating fishing boats during normal trawling operations (May 2014-May 2015; Gulf of Maine, Georges Bank). Conventional methods observed 51 different prey taxa and nearly 1,600 individual prey items, but no cod were observed. Cod DNA was detected in 31 (10.5%) of the dogfish stomachs, with a higher percentage of these from the homogenate of amorphous, well-digested prey and stomach fluids (20 stomachs or 65%) than from discrete animal tissues (11 stomachs or 35%). Re-examination of photographs of these 11 tissue samples revealed one whole, partially digested fish that could be recognized in hindsight as cod.
Cod DNA was observed in dogfish stomachs year round: in January (1 of 1 trip), February (1 of 1), May (1 of 3), June (0 of 1), July (3 of 4), August (1 of 2), and October (3 of 3). Although these data suggest higher interaction rates between dogfish and cod than previously observed, addressing the population consequences of this predator-prey relationship requires a robust sampling design, estimates of digestion rates by dogfish to account for complete degradation of DNA sequences, and consideration for dogfish scavenging during fishing operations.
Multiple PCR assay based on the cigR gene for detection of Salmonella spp. and Salmonella Pullorum/Gallinarum identification
Salmonella spp. are important zoonotic pathogens that are responsible for severe diseases in both animals and humans. Salmonella enterica subsp. enterica serovar Gallinarum biovar Gallinarum (S. Gallinarum) and biovar Pullorum (S. Pullorum) are typical infectious pathogens detected in the chicken industry that have caused great economic losses. To facilitate their detection and prevent contamination, we developed a rapid multiple PCR method, which can simultaneously detect Salmonella spp. and further identify the biovars S. Pullorum/Gallinarum. This PCR detection method is based on the cigR gene, which is conserved among Salmonella spp. but has a 42-bp deletion in S. Pullorum/Gallinarum. The specificity and sensitivity of the PCR assay was evaluated with 41 different strains: 34 Salmonella strains, including 5 S. Pullorum/Gallinarum strains, and 7 non-Salmonella strains.
The lower limit of detection was 8.15 pg of S. Pullorum (S06004) genomic DNA and 20 cfu in PCR, which shows a great sensitivity. In addition, this method was applied to detect or identify Salmonella from processing chicken liver and egg samples, and the results corresponded to those obtained from serotype analysis using the conventional slide agglutination test. Overall, the new cigR-based PCR assay is efficient and practical for Salmonella detection and S. Pullorum/Gallinarum identification and will greatly reduce the workload of epidemiologic investigation.
Virus Zika PCR kit |
PCR-H434-96R |
Bioingentech |
100T |
EUR 1335.6 |
Virus Zika One-Step PCR kit |
Oneq-H434-100R |
Bioingentech |
100T |
EUR 1932 |
Virus Zika One-Step PCR kit |
Oneq-H434-150R |
Bioingentech |
150T |
EUR 2646 |
Virus Zika One-Step PCR kit |
Oneq-H434-50R |
Bioingentech |
50T |
EUR 1410 |
Zika Virus (ZIKV) Envelop protein ELISA kit for vaccine testing, 48 tests, Quantitative |
RV-403001-ENV-48 |
Alpha Diagnostics |
1 Kit |
EUR 1000.8 |
Zika Virus NS1 (ZIKV-NS1) protein ELISA kit for vaccine testing, 96 tests, Quantitative |
RV-403301-NS1-48 |
Alpha Diagnostics |
1 Kit |
EUR 1000.8 |
Zika Virus NS1 antigen |
00346-V-01mg |
Virogen |
0,1 mg |
EUR 321 |
|
Description: Zika Virus NS1 antigen 34 kDa |
Zika Virus NS1 antigen |
00346-V-1000ug |
Virogen |
1000 ug |
EUR 1539 |
|
Description: Zika Virus NS1 antigen 34 kDa |
Zika virus NS1 protein |
30-1931 |
Fitzgerald |
100 ug |
EUR 613.2 |
Description: Recombinant Zika virus NS1 protein |
Zika virus NS1 protein |
30-1936 |
Fitzgerald |
100 ug |
EUR 289.2 |
Description: Recombinant Zika virus NS1 protein |
Zika Virus NS1 Protein |
abx061380-1mg |
Abbexa |
1 mg |
EUR 1722 |
|
Zika virus NS1 antibody |
10-2707 |
Fitzgerald |
1 mg |
EUR 678 |
Description: Mouse monoclonal Zika virus NS1 antibody |
Zika virus NS1 antibody |
10-2708 |
Fitzgerald |
1 mg |
EUR 678 |
Description: Mouse monoclonal Zika virus NS1 antibody |
Zika virus NS1 antibody |
10-2709 |
Fitzgerald |
1 mg |
EUR 678 |
Description: Mouse monoclonal Zika virus NS1 antibody |
Zika virus NS1 antibody |
10-2710 |
Fitzgerald |
1 mg |
EUR 678 |
Description: Mouse monoclonal Zika virus NS1 antibody |
Zika virus NS1 antibody |
10-2711 |
Fitzgerald |
1 mg |
EUR 678 |
Description: Mouse monoclonal Zika virus NS1 antibody |
Zika Virus NS1 Antibody |
abx024134-1mg |
Abbexa |
1 mg |
EUR 1730.4 |
|
Zika Virus NS1 Antibody |
abx024135-1mg |
Abbexa |
1 mg |
EUR 1730.4 |
|
Zika Virus NS1 Antibody |
abx024136-1mg |
Abbexa |
1 mg |
EUR 1730.4 |
|
Zika Virus NS1 Antibody |
abx024137-1mg |
Abbexa |
1 mg |
EUR 1730.4 |
|
Zika Virus NS1 Antibody |
abx024138-1mg |
Abbexa |
1 mg |
EUR 1730.4 |
|
Zika Virus NS1 Antibody |
abx024139-1mg |
Abbexa |
1 mg |
EUR 1730.4 |
|
Zika Virus NS1 Antibody |
abx024140-1mg |
Abbexa |
1 mg |
EUR 1730.4 |
|
Zika Virus NS1 Antibody |
abx024141-1mg |
Abbexa |
1 mg |
EUR 1730.4 |
|
Zika Virus NS1 Antibody |
abx024142-1mg |
Abbexa |
1 mg |
EUR 1730.4 |
|
Zika Virus NS1 Antibody |
abx024143-1mg |
Abbexa |
1 mg |
EUR 1730.4 |
|
Zika Virus NS1 Antibody |
abx024144-1mg |
Abbexa |
1 mg |
EUR 1730.4 |
|
Zika Virus NS1 Antibody |
abx024159-1mg |
Abbexa |
1 mg |
EUR 727.2 |
|
Zika Virus NS1 Antibody |
abx024160-1mg |
Abbexa |
1 mg |
EUR 1212 |
|
Zika virus NS1 antibody |
70-1086 |
Fitzgerald |
500ug |
EUR 366 |
Description: Purified Goat polyclonal Zika virus NS1 antibody |
Zika virus NS1 antibody |
70-1087 |
Fitzgerald |
500 ug |
EUR 638.4 |
Description: Affinity purified goat polyclonal antibody |
Zika virus envelope protein |
30-1932 |
Fitzgerald |
100 ug |
EUR 457.2 |
Description: Recombinant Zika virus envelope protein |
Zika virus envelope protein |
30-1935 |
Fitzgerald |
100 ug |
EUR 289.2 |
Description: Recombinant Zika virus envelope protein |
Zika Virus Envelope Protein |
abx061381-1mg |
Abbexa |
1 mg |
EUR 1722 |
|
Zika Virus Envelope Protein |
abx160022-1mg |
Abbexa |
1 mg |
EUR 1746 |
|
Zika virus envelope antibody |
10-2712 |
Fitzgerald |
1 mg |
EUR 678 |
Description: Mouse monoclonal Zika virus envelope antibody |
Zika virus envelope antibody |
10-2713 |
Fitzgerald |
1 mg |
EUR 678 |
Description: Mouse monoclonal Zika virus envelope antibody |
Zika virus envelope antibody |
10-2714 |
Fitzgerald |
1 mg |
EUR 678 |
Description: Mouse monoclonal Zika virus envelope antibody |
Zika virus envelope antibody |
10-2715 |
Fitzgerald |
1 mg |
EUR 678 |
Description: Mouse monoclonal Zika virus envelope antibody |
Zika Virus Envelope Antibody |
abx024128-1mg |
Abbexa |
1 mg |
EUR 1730.4 |
|
Zika Virus Envelope Antibody |
abx024129-1mg |
Abbexa |
1 mg |
EUR 1730.4 |
|
Zika Virus Envelope Antibody |
abx024130-1mg |
Abbexa |
1 mg |
EUR 1730.4 |
|
Zika Virus Envelope Antibody |
abx024131-1mg |
Abbexa |
1 mg |
EUR 1730.4 |
|
Zika Virus Envelope Antibody |
abx024132-1mg |
Abbexa |
1 mg |
EUR 1696.8 |
|
Zika Virus Envelope Antibody |
abx024133-1mg |
Abbexa |
1 mg |
EUR 1696.8 |
|
0.2ML CLEAR STRIP CAPS FOR REAL TIME PCR |
PCR-2CP-RT-C |
CORNING |
125/pk |
EUR 267.6 |
Description: PCR Plates & Tubes; PCR Strip Tubes and Strip Caps - Axygen |
Inactivated Zika Virus Antigen |
VAng-Lsx0574-1mL |
Creative Biolabs |
1 mL |
EUR 2510.4 |
Description: Zika virus, inactivated natural antigen. |
Custom Testing of Samples for Antibodies (IgA/IgG/IgM) to Zika Virus (ZIKV) vaccines (Env/NS1/prM/Capsid) by ELISA |
RV-403001-CUX |
Alpha Diagnostics |
Custom |
Ask for price |
Recombinant Zika Virus E Protein |
VAng-Lsx0581-100g |
Creative Biolabs |
100 µg |
EUR 1365.6 |
Description: Zika Virus Envelope, recombinant protein from Insect cells. |
Novel Coronavirus COVID-19 (2019-nCoV) Real Time RT-PCR Kit |
RR-0478-02 |
Liferiver |
25 tests/kit |
EUR 1189.2 |
|
Description: Novel Coronavirus (2019-nCoV) Real Time RT-PCR Kit is used for the qualitative detection of a novel coronavirus, which was identified in 2019 at Wuhan City, Hubei Province, China, in upper respiratory tract specimens (nasopharyngeal extracts, deep cough sputum, etc.) and lower respiratory tract specimens (alveoli irrigation fluid, etc.) by real time PCR systems. |
Zika Virus NS1 antigen 34 kDa |
00346-V-100ugvial |
Virogen |
100 ug/vial |
EUR 150 |
Description: Zika Virus NS1 antigen 34 kDa |
Zika Virus NS1 Recombinant Protein |
97-074 |
ProSci |
0.05 mg |
EUR 752.1 |
Description: Zika virus (ZIKV) is a member of the virus family Flaviviridae and the genus Flavivirus, transmitted by daytime-active Aedes mosquitoes, such as A. aegypti and A. albopictus. Its name comes from the Zika Forest of Uganda, where the virus was first isolated in 1947. Zika virus is related to dengue, yellow fever, Japanese encephalitis, and West Nile viruses. The infection, known as Zika fever, often causes no or only mild symptoms, similar to a mild form of dengue fever. It is treated by rest. Since the 1950s, it has been known to occur within a narrow equatorial belt from Africa to Asia. As of 2016, the illness cannot be prevented by drugs or vaccines. As of February 2016, there is evidence that Zika fever in pregnant women is associated with abnormal brain development in their fetuses through mother-to-child transmission of the virus, which may result in miscarriage or microcephaly. |
Recombinant Zika virus NS1 Protein |
P1064-10 |
Biovision |
each |
EUR 189.6 |
Recombinant Zika virus NS1 Protein |
P1064-50 |
Biovision |
each |
EUR 366 |
Mouse antibody for Zika virus NS1 |
5122 |
Virostat |
100 ug |
EUR 405.83 |
Description: This is purified Mouse monoclonal antibody against Zika virus NS1 for WB, ELISA. |
Mouse antibody for Zika virus NS1 |
5123 |
Virostat |
100 ug |
EUR 405.83 |
Description: This is purified Mouse monoclonal antibody against Zika virus NS1 for WB, ELISA. |
Mouse antibody for Zika virus NS1 |
5124 |
Virostat |
100 ug |
EUR 405.83 |
Description: This is purified Mouse monoclonal antibody against Zika virus NS1 for WB, ELISA. |
Mouse antibody for Zika virus NS1 |
5125 |
Virostat |
100 ug |
EUR 405.83 |
Description: This is purified Mouse monoclonal antibody against Zika virus NS1 for WB, ELISA. |
Zika virus Envelope recombinant antigen |
8826 |
Virostat |
100 ug |
EUR 673.36 |
Description: This is Zika virus Envelope recombinant antigen for ELISA,WB. |
Zika Virus ENV-NS1 fusion antigen |
00345-V-01mg |
Virogen |
0,1 mg |
EUR 321 |
|
Description: Zika Virus ENV -NS1 fusion antigen 54 kDa |
Zika Virus ENV-NS1 fusion antigen |
00345-V-1000ug |
Virogen |
1000 ug |
EUR 1539 |
|
Description: Zika Virus ENV -NS1 fusion antigen 54 kDa |
Human CellExp? Zika virus NS1 Protein |
P1062-10 |
Biovision |
each |
EUR 314.4 |
ELISA kit for Human Zika Virus IgG (ZV-IgG) |
KTE62724-48T |
Abbkine |
48T |
EUR 424.8 |
Description: Quantitative sandwich ELISA for measuring Human Zika Virus IgG (ZV-IgG) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
ELISA kit for Human Zika Virus IgG (ZV-IgG) |
KTE62724-5platesof96wells |
Abbkine |
5 plates of 96 wells |
EUR 2702.4 |
Description: Quantitative sandwich ELISA for measuring Human Zika Virus IgG (ZV-IgG) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
ELISA kit for Human Zika Virus IgG (ZV-IgG) |
KTE62724-96T |
Abbkine |
96T |
EUR 686.4 |
Description: Quantitative sandwich ELISA for measuring Human Zika Virus IgG (ZV-IgG) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
ELISA kit for Human Zika Virus IgM (ZV-IgM) |
KTE63006-48T |
Abbkine |
48T |
EUR 424.8 |
Description: Quantitative sandwich ELISA for measuring Human Zika Virus IgM (ZV-IgM) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
ELISA kit for Human Zika Virus IgM (ZV-IgM) |
KTE63006-5platesof96wells |
Abbkine |
5 plates of 96 wells |
EUR 2702.4 |
Description: Quantitative sandwich ELISA for measuring Human Zika Virus IgM (ZV-IgM) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
ELISA kit for Human Zika Virus IgM (ZV-IgM) |
KTE63006-96T |
Abbkine |
96T |
EUR 686.4 |
Description: Quantitative sandwich ELISA for measuring Human Zika Virus IgM (ZV-IgM) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
Zika virus NS1/Env. recombinant antigen |
8827 |
Virostat |
100 ug |
EUR 673.36 |
Description: This is Zika virus NS1/Env. recombinant antigen for ELISA,WB. |
Recombivirus? Human Anti-Zika Virus (ZIKV) PrM protein IgG ELISA kit, 96 tests, Quantitative |
RV-403200-1 |
Alpha Diagnostics |
1 Kit |
EUR 1074 |
Recombivirus? Human Anti-Zika Virus (ZIKV) PrM protein IgG1 ELISA kit, 96 tests, Quantitative |
RV-403200-HG1 |
Alpha Diagnostics |
1 Kit |
EUR 1220.4 |
Recombivirus? Human Anti-Zika Virus (ZIKV) PrM protein IgG2 ELISA kit, 96 tests, Quantitative |
RV-403200-HG2 |
Alpha Diagnostics |
1 Kit |
EUR 1220.4 |
Recombivirus? Human Anti-Zika Virus (ZIKV) PrM protein IgM ELISA kit, 96 tests, Quantitative |
RV-403205-1 |
Alpha Diagnostics |
1 Kit |
EUR 1074 |
Recombivirus? Monkey Anti-Zika Virus (ZIKV) PrM protein IgG ELISA kit, 96 tests, Quantitative |
RV-403210-1 |
Alpha Diagnostics |
1 Kit |
EUR 1074 |
Recombivirus? Monkey Anti-Zika Virus (ZIKV) PrM protein IgM ELISA kit, 96 tests, Quantitative |
RV-403215-1 |
Alpha Diagnostics |
1 Kit |
EUR 1074 |
Recombivirus? Mouse Anti-Zika Virus (ZIKV) PrM protein IgG ELISA kit, 96 tests, Quantitative |
RV-403220-1 |
Alpha Diagnostics |
1 Kit |
EUR 1074 |
Recombivirus? Mouse Anti-Zika Virus (ZIKV) PrM protein IgG1 ELISA kit, 96 tests, Quantitative |
RV-403220-MG1 |
Alpha Diagnostics |
1 Kit |
EUR 1220.4 |
Recombivirus? Mouse Anti-Zika Virus (ZIKV) PrM protein IgM ELISA kit, 96 tests, Quantitative |
RV-403225-1 |
Alpha Diagnostics |
1 Kit |
EUR 1074 |
Recombivirus? Rabbit Anti-Zika Virus (ZIKV) prM protein IgG ELISA kit, 96 tests, Quantitative |
RV-403230-1 |
Alpha Diagnostics |
1 Kit |
EUR 1074 |
Recombivirus? Rabbit Anti-Zika Virus (ZIKV) prM protein IgM ELISA kit, 96 tests, Quantitative |
RV-403235-1 |
Alpha Diagnostics |
1 Kit |
EUR 1074 |
Recombivirus? Human Anti-Zika Virus (ZIKV) NS1 protein IgG ELISA kit, 96 tests, Quantitative |
RV-403300-1 |
Alpha Diagnostics |
1 Kit |
EUR 1074 |
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