DNA recovery from Droplet Digital PCR emulsions using liquid nitrogen

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Analysis of the Molecular Diversity Among Cronobacter Species Isolated From Filth Flies Using Targeted PCR, Pan Genomic DNA Microarray, and Whole Genome Sequencing Analyses

 

Cronobacter species are opportunistic pathogens capable of causing life-threatening infections in humans, with serious complications arising in neonates, infants, immuno-compromised individuals, and elderly adults. The genus is comprised of seven species: Cronobacter sakazakii, Cronobacter malonaticus, Cronobacter turicensis, Cronobacter muytjensii, Cronobacter dublinensis, Cronobacter universalis, and Cronobacter condimenti. Despite a multiplicity of genomic data for the genus, little is known about likely transmission vectors. Using DNA microarray analysis, in parallel with whole genome sequencing, and targeted PCR analyses, the total gene content of two C. malonaticus, three C. turicensis, and 14 C. sakazaki isolated from various filth flies was assessed.
Phylogenetic relatedness among these and other strains obtained during surveillance and outbreak investigations were comparatively assessed. Specifically, microarray analysis (MA) demonstrated its utility to cluster strains according to species-specific and sequence type (ST) phylogenetic relatedness, and that the fly strains clustered among strains obtained from clinical, food and environmental sources from United States, Europe, and Southeast Asia. This combinatorial approach was useful in data mining for virulence factor genes, and phage genes and gene clusters. In addition, results of plasmidotyping were in agreement with the species identity for each strain as determined by species-specific PCR assays, MA, and whole genome sequencing.
Microarray and BLAST analyses of Cronobacter fly sequence datasets were corroborative and showed that the presence and absence of virulence factors followed species and ST evolutionary lines even though such genes were orthologous. Additionally, zebrafish infectivity studies showed that these pathotypes were as virulent to zebrafish embryos as other clinical strains. In summary, these findings support a striking phylogeny amongst fly, clinical, and surveillance strains isolated during 2010-2015, suggesting that flies are capable vectors for transmission of virulent Cronobacter spp.; they continue to circulate among United States and European populations, environments, and that this “pattern of circulation” has continued over decades.

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DNA recovery from Droplet Digital PCR emulsions using liquid nitrogen

 

 

Droplet microfluidics is a technology that enables the production and manipulation of small volumes. In biosciences, the most popular application of this technology is Droplet Digital™ PCR (ddPCR™), where parallel nanoliter-scale PCR assays are used to provide a high sensitivity and specificity for DNA detection.

 

However, the recovery of PCR products for downstream applications such as sequencing can be challenging due to the droplets’ stability. Here we compared five methods for disrupting the droplets to recover DNA. We found that rapid freezing in liquid nitrogen results in a clear phase separation and recovery of up to 70% of the DNA content. Liquid nitrogen freezing can thus offer a simple and environmentally friendly protocol for recovering DNA from ddPCR.

 

Classification and review of free PCR primer design software

 

Motivation: Polymerase chain reaction (PCR) has been a revolutionary biomedical advancement. However, for PCR to be appropriately used, one must spend a significant amount of effort on PCR primer design. Carefully designed PCR primers not only increase sensitivity and specificity, but also decrease effort spent on experimental optimization. Computer software removes the human element by performing and automating the complex and rigorous calculations required in PCR primer design. Classification and review of the available software options and their capabilities should be a valuable resource for any PCR application.
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Results: This paper focuses on currently available free PCR primer design software and their major functions (https://pcrprimerdesign.github.io/). The software are classified according to their PCR applications, such as Sanger sequencing, reverse transcription quantitative PCR, single nucleotide polymorphism detection, splicing variant detection, methylation detection, microsatellite detection, multiplex PCR and targeted next generation sequencing, and conserved/degenerate primers to clone orthologous genes from related species, new gene family members in the same species, or to detect a group of related pathogens. Each software is summarized to provide a technical review of their capabilities and utilities.

Sensitive detection and quantification of SARS-CoV-2 by multiplex droplet digital RT-PCR

 

The purpose of this study is to develop a one-step droplet digital RT-PCR (RT-ddPCR) multiplex assay that allows for sensitive quantification of SARS-CoV-2 RNA with respect to human-derived RNA and could be used for screening and monitoring of Covid-19 patients. A one-step RT-ddPCR multiplex assay was developed for simultaneous detection of SARS-CoV-2 E, RdRp and N viral RNA, and human Rpp30 DNA and GUSB mRNA, for internal nucleic acid (NA) extraction and RT-PCR control. Dilution series of viral RNA transcripts were prepared in water and total NA extract of Covid-19-negative patients. As reference assay, an E-GUSB duplex RT-PCR was used.

 

GUSB mRNA detection was used to set validity criteria to assure viral RNA and RT-PCR assay quality and to enable quantification of SARS-CoV-2 RNA. In a background of at least 100 GUSB mRNA copies, 5 copies of viral RNA are reliably detectable and 10 copies viral RNA copies are reliably quantifiable. It was found that assay sensitivity of the RT-ddPCR was not affected by the total NA background while assay sensitivity of the gold standard RT-PCR assay is drastically decreased when SARS-CoV-2 copies were detected in a background of total NA extract compared with water. The present study describes a robust and sensitive one-step ddRT-PCR multiplex assay for reliable quantification of SARS-CoV-2 RNA. By determining the fractional abundance of viral RNA with respect to a human housekeeping gene, viral loads from different samples can be compared, what could be used to investigate the infectiveness and to monitor Covid-19 patients.

 

rRT-PCR Results of a Covid-19 Diagnosed Geriatric Patient

 

In this study, we aimed to present a geriatric patient with the diagnosis of COVID-19 and with contradictory results in rRT-PCR examinations in short time intervals. A 69-year-old male patient was admitted to the emergency room on the 18th day of May 2020, with the complaints of fever, sweating, myalgia, dry cough that continued for 5 days, and the lack of taste that started on the day he applied to the emergency room. Comorbidity factors include diabetes mellitus, bronchial asthma, and hypertension. The patient has a history of 36 years of smoking 1.5 packs per day. High laboratory findings during hospitalization: monocytes, creatinine, CRP (C-reactive protein). In the thorax CT, in the parenchyma areas of both lungs, there are increases in attenuation with multilobe distributions (more visible at the level of the upper lobes) in the form of ground-glass opacities.

 

May 19, 2020, was subjected to the rRT-PCR test, repeated twice on the 19th of May which also resulted in positive. Despite rRT-PCR tests, which were negative on 27th of May and positive on 28th of May, the patient, whose symptoms disappeared, and general condition improved, was discharged on June 1, 2020, with the recommendation for home isolation. In our case, unlike the incubation period only, we encountered a negative rRT-PCR result on the 8th day after diagnosis. Therefore, the COVID-19 pandemic control and filiation evaluation with the rRT-PCR test may produce false negative results.

 

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EUR 1858.37
Description: Please contact Gentaur in order to receive the datasheet of the product.

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HCV10165 9 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 70225 (5 X 1 mL)

HCV10185 5 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 61025 (5 X 1 mL)

HCV6217 5 X 1 mL
EUR 1035

HCV Seroconversion Panel Donor# 61251 (8 X 1 mL)

HCV6218 8 X 1 mL
EUR 1035

HCV Seroconversion Panel Donor# 60749 (14 X 1 mL)

HCV6219 14 X 1 mL
EUR 1035

HCV Seroconversion Panel Donor# 63792 (6 X 1 mL)

HCV6220 6 X 1 mL
EUR 1035

HCV Seroconversion Panel Donor# 62258 (8 X 1 mL)

HCV6221 8 X 1 mL
EUR 1035

HCV Seroconversion Panel Donor# 62286 (8 X 1 mL)

HCV6222 8 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 62507 (22 X 1 mL)

HCV6223 22 X 1 mL
EUR 1035

HCV Seroconversion Panel Donor# 62680 (6 X 1 mL)

HCV6224 6 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 62999 (19 X 1 mL)

HCV6225 19 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 63135 (12 X 1 mL)

HCV6226 12 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 63318 (7 X 1 mL)

HCV6227 7 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 62886 (12 X 1 mL)

HCV6228 12 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 62804 (8 X 1 mL)

HCV6229 8 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 63625 (8 X 1 mL)

HCV9041 8 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 63792 (6 X 1 mL)

HCV9042 6 X 1 mL
EUR 1035

HCV Seroconversion Panel Donor# 63869 (6 X 1 mL)

HCV9043 6 X 1 mL
EUR 1035

HCV Seroconversion Panel Donor# 63911 (6 X 1 mL)

HCV9044 6 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 64150 (8 X 1 mL)

HCV9045 8 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 64254 (5 X 1 mL)

HCV9046 5 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 64273 (10 X 1 mL)

HCV9047 10 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 64437 (8 X 1 mL)

HCV9048 8 X 1 mL
EUR 1035

HCV Seroconversion Panel Donor# 64766 (5 X 1 mL)

HCV9049 5 X 1 mL
EUR 1035

HCV Seroconversion Panel Donor# 66158 (13 X 1 mL)

HCV9050 13 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 66626 (8 X 1 mL)

HCV9054 8 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 66732 (11 X 1 mL)

HCV9055 11 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 66861 (11 X 1 mL)

HCV9057 11 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 66647 (5 X 1 mL)

HCV9058 5 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 1038851 (5 X 1 mL)

HCV9095 5 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 22708D (5 X 1 mL)

HCV9094 5 X 1 mL
EUR 1764

HCV Pre/Post Seroconversion Panel Donor# 66822 (5 X 1 mL)

HCV10235 5 X 1 mL
EUR 545

HIV Seroconversion Panel Donor# 65404 (7 X mL)

HIV9013 7 X mL
EUR 1764

EBV Seroconversion Panel Donor# 283843421 (14 X 1 mL)

EBV9097 14 X 1 mL
EUR 1764

HIV Seroconversion Panel Donor# 73695 (9 X 1 mL)

HIV12007 9 X 1 mL
EUR 2344

HIV Seroconversion Panel Donor# 73698 (13 X 1 mL)

HIV12008 13 X 1 mL
EUR 2344

HIV Seroconversion Panel Donor# 63331 (7 X 1 mL)

HIV6248 7 X 1 mL
EUR 1764

HIV Seroconversion Panel Donor# 64954 (11 X 1 mL)

HIV9011 11 X 1 mL
EUR 1764

HIV Seroconversion Panel Donor# 65389 (8 X 1 mL)

HIV9012 8 X 1 mL
EUR 1764

HIV Seroconversion Panel Donor# 65522 (5 X 1 mL)

HIV9014 5 X 1 mL
EUR 1764

HIV Seroconversion Panel Donor# 65661 (6 X 1 mL)

HIV9015 6 X 1 mL
EUR 1858.37
Description: Please contact Gentaur in order to receive the datasheet of the product.

HIV Seroconversion Panel Donor# 65790 (10 X 1 mL)

HIV9016 10 X 1 mL
EUR 1764

HIV Seroconversion Panel Donor# 66575 (10 X 1 mL)

HIV9018 10 X 1 mL
EUR 1764

HIV Seroconversion Panel Donor# 65685 (3 X 1 mL)

HIV9019 3 X 1 mL
EUR 1764

HIV Seroconversion Panel Donor# 67512 (22 X 1 mL)

HIV9020 22 X 1 mL
EUR 1764

HIV Seroconversion Panel Donor# 67485 (17 X 1 mL)

HIV9021 17 X 1 mL
EUR 1764

HIV Seroconversion Panel Donor# 64578 (8 X 1 mL)

HIV9022 8 X 1 mL
EUR 1764

HIV Seroconversion Panel Donor# 67706 (22 X 1 mL)

HIV9023 22 X 1 mL
EUR 1764

HIV Seroconversion Panel Donor# 64469 (12 X 1 mL)

HIV9024 12 X 1 mL
EUR 1764

HIV Seroconversion Panel Donor# 67996 (12 X 1 mL)

HIV9025 12 X 1 mL
EUR 1764

HIV Seroconversion Panel Donor# 68205 (7 X 1 mL)

HIV9026 7 X 1 mL
EUR 1764

HIV Seroconversion Panel Donor# 67860 (7 X 1 mL)

HIV9028 7 X 1 mL
EUR 1764

HIV Seroconversion Panel Donor# 68262 (12 X 1 mL)

HIV9029 12 X 1 mL
EUR 1764

HIV Seroconversion Panel Donor# 68582 (16 X 1 mL)

HIV9030 16 X 1 mL
EUR 1764

HIV Seroconversion Panel Donor# 66048 (19 X 1 mL)

HIV9031 19 X 1 mL
EUR 1764

HIV Seroconversion Panel Donor# 66686 (16 X 1 mL)

HIV9033 16 X 1 mL
EUR 1764

HIV Seroconversion Panel Donor# 66632 (13 X 1 mL)

HIV9034 13 X 1 mL
EUR 1764

HIV Seroconversion Panel Donor# 62216 (4 X 1 mL)

HIV9075 4 X 1 mL
EUR 2344

HIV Seroconversion Panel Donor# 63753 (9 X 1 mL)

HIV9076 9 X 1 mL
EUR 2344

HIV Seroconversion Panel Donor# 75018 (23 X 1 mL)

HIV9077 23 X 1 mL
EUR 2344

HIV Seroconversion Panel Donor# 79065 (5 X 1 mL)

HIV9078 5 X 1 mL
EUR 2344

HIV Seroconversion Panel Donor# 75062 (15 X 1 mL)

HIV9079 15 X 1 mL
EUR 2344

HIV Seroconversion Panel Donor# 63215 (4 X 1 mL)

HIV9081 4 X 1 mL
EUR 2344

HIV Seroconversion Panel Donor# 77840 (4 X 1 mL)

HIV9082 4 X 1 mL
EUR 1764

HIV Seroconversion Panel Donor# 77600 (4 X 1 mL)

HIV9084 4 X 1 mL
EUR 1764

HIV Seroconversion Panel Donor# 65376 (6 X 1 mL)

HIV9089 6 X 1 mL
EUR 1764

HIV Seroconversion Panel Donor# 1043487 (6 X 1 mL)

HIV9096 6 X 1 mL
EUR 1764

HBV Seroconversion Panel Donor# 64090 (5 X 1 mL)

HBV10231 5 X 1 mL
EUR 627

HBV Seroconversion Panel Donor# 64017 (6 X 1 mL)

HBV10232 6 X 1 mL
EUR 627

HBV Seroconversion Panel Donor# 65449 (9 X 1 mL)

HBV11000 9 X 1 mL
EUR 953

HBV Seroconversion Panel Donor# 65524 (8 X 1 mL)

HBV11001 8 X 1 mL
EUR 953

HBV Seroconversion Panel Donor# 65556 (6 X 1 mL)

HBV11002 6 X 1 mL
EUR 953

HBV Seroconversion Panel Donor# 65584 (8 X 1 mL)

HBV11003 8 X 1 mL
EUR 953

HBV Seroconversion Panel Donor# 65732 (8 X 1 mL)

HBV11004 8 X 1 mL
EUR 953

HBV Seroconversion Panel Donor# 65777 (14 X 1 mL)

HBV11005 14 X 1 mL
EUR 953

HBV Seroconversion Panel Donor# 66201 (17 X 1 mL)

HBV11006 17 X 1 mL
EUR 953

HBV Seroconversion Panel Donor# 66537 (14 X 1 mL)

HBV11007 14 X 1 mL
EUR 953

HBV Seroconversion Panel Donor# 67303 (18 X 1 mL)

HBV11008 18 X 1 mL
EUR 953

HBV Seroconversion Panel Donor# 67449 (23 X 1 mL)

HBV11009 23 X 1 mL
EUR 953

HBV Seroconversion Panel Donor# 67457 (18 X 1 mL)

HBV11010 18 X 1 mL
EUR 953

HBV Seroconversion Panel Donor# 67694 (14 X 1 mL)

HBV11011 14 X 1 mL
EUR 953

HBV Seroconversion Panel Donor# 67773 (6 X 1 mL)

HBV11012 6 X 1 mL
EUR 953

HBV Seroconversion Panel Donor# 67962 (35 X 1 mL)

HBV11013 35 X 1 mL
EUR 953

HBV Seroconversion Panel Donor# 68029 (12 X 1 mL)

HBV11014 12 X 1 mL
EUR 953

HBV Seroconversion Panel Donor# 68105 (14 X 1 mL)

HBV11015 14 X 1 mL
EUR 953

HBV Seroconversion Panel Donor# 68541 (10 X 1 mL)

HBV11016 10 X 1 mL
EUR 953

HBV Seroconversion Panel Donor# 68739 (14 X 1 mL)

HBV11017 14 X 1 mL
EUR 953

HBV Seroconversion Panel Donor# 70793 (14 X 1 mL)

HBV11024 14 X 1 mL
EUR 953

HBV Seroconversion Panel Donor# 70970 (16 X 1 mL)

HBV11026 16 X 1 mL
EUR 953

HBV Seroconversion Panel Donor# 71415 (13 X 1 mL)

HBV11027 13 X 1 mL
EUR 953

HBV Seroconversion Panel Donor# 71840 (10 X 1 mL)

HBV11028 10 X 1 mL
EUR 953

HBV Seroconversion Panel Donor# 69751 (13 X 1 mL)

HBV11029 13 X 1 mL
EUR 953

HBV Seroconversion Panel Donor# 70292 (15 X 1 mL)

HBV11031 15 X 1 mL
EUR 953

HBV Seroconversion Panel Donor# 72439 (13 X 1 mL)

HBV11052 13 X 1 mL
EUR 953

HBV Seroconversion Panel Donor# 72474 (11 X 1 mL)

HBV11056 11 X 1 mL
EUR 953

HBV Seroconversion Panel Donor# 71612 (8 X 1 mL)

HBV11058 8 X 1 mL
EUR 953

HBV Seroconversion Panel Donor# 72324 (9 X 1 mL)

HBV11059 9 X 1 mL
EUR 953

HBV Seroconversion Panel Donor# 71922 (11 X 1 mL)

HBV11062 11 X 1 mL
EUR 953

HBV Seroconversion Panel Donor# 71782 (9 X 1 mL)

HBV11064 9 X 1 mL
EUR 953

HBV Seroconversion Panel Donor# 73299 (15 X 1 mL)

HBV11069 15 X 1 mL
EUR 953

HBV Seroconversion Panel Donor# 61248 (5 X 1 mL)

HBV6271 5 X 1 mL
EUR 953

HBV Seroconversion Panel Donor# 61291 (25 X 1 mL)

HBV6272 25 X 1 mL
EUR 953

HBV Seroconversion Panel Donor# 61042 (6 X 1 mL)

HBV6273 6 X 1 mL
EUR 953

HBV Seroconversion Panel Donor# 61799 (7 X 1 mL)

HBV6274 7 X 1 mL
EUR 953

HBV Seroconversion Panel Donor# 61066 (7 X 1 mL)

HBV6275 7 X 1 mL
EUR 953

HBV Seroconversion Panel Donor# 60409 (8 X 1 mL)

HBV6276 8 X 1 mL
EUR 953

HBV Seroconversion Panel Donor# 63291 (11 X 1 mL)

HBV6277 11 X 1 mL
EUR 953

HBV Seroconversion Panel Donor# 63426 (10 X 1 mL)

HBV6278 10 X 1 mL
EUR 1030.94
Description: Please contact Gentaur in order to receive the datasheet of the product.

HBV Seroconversion Panel Donor# 63701 (7 X 1 mL)

HBV6279 7 X 1 mL
EUR 953

HBV Seroconversion Panel Donor# 61832 (5 X 1 mL)

HBV6280 5 X 1 mL
EUR 953

HBV Seroconversion Panel Donor# 62433 (12 X 1 mL)

HBV6281 12 X 1 mL
EUR 1937

HBV Seroconversion Panel Donor# 62675 (14 X 1 mL)

HBV6282 14 X 1 mL
EUR 953

HBV Seroconversion Panel Donor# 62825 (11 X 1 mL)

HBV6283 11 X 1 mL
EUR 953

HBV Seroconversion Panel Donor# 62347 (19 X 1 mL)

HBV6284 19 X 1 mL
EUR 953

HBV Seroconversion Panel Donor# 62967 (16 X 1 mL)

HBV6285 16 X 1 mL
EUR 953

HBV Seroconversion Panel Donor# 63133 (9 X 1 mL)

HBV6286 9 X 1 mL
EUR 953

HBV Seroconversion Panel Donor# 63253 (11 X 1 mL)

HBV6287 11 X 1 mL
EUR 953

 

Flat Replaceable Tips 13 mm Diameter Flat Titanium Tip

0-120-0016 13 mm
EUR 86
Description: use with corresponding Tapped Tips and Tapped Extender Tips

Flat Replaceable Tips 19 mm Diameter Flat Titanium Tip

0-120-0017 19 mm
EUR 92
Description: use with corresponding Tapped Tips and Tapped Extender Tips

Flat Replaceable Tips 25 mm Diameter Flat Titanium Tip

0-120-0018 25 mm
EUR 100
Description: use with corresponding Tapped Tips and Tapped Extender Tips

Titanium Cup Tip, 250 ml

0-120-0019 250 ml
EUR 1377
Description: includes Interface Washers

Microtube Tray, 8 Position (for 250 ml Cup Tip)

0-120-0021 each
EUR 113
Description: includes Interface Washers

Continuous Flow Chamber

0-120-0026 each
EUR 774
Description: includes Interface Washers

Interface Washers 1.5” Diameter, 5/Pkg

0-120-003 1.5'' Diameter
EUR 31

13 mm Diameter Solid Titanium Extender Tip

0-120-0032 13 mm
EUR 301

13 mm Diameter Tapped Titanium Extender Tip

0-120-0033 13 mm
EUR 362

19 mm Diameter Solid Titanium Extender Tip

0-120-0034 19 mm
EUR 315

19 mm Diameter Tapped Titanium Extender Tip

0-120-0035 19 mm
EUR 375

25 mm Diameter Solid Titanium Extender Tip

0-120-0036 25 mm
EUR 328

25 mm Diameter Tapped Titanium Extender Tip

0-120-0037 25 mm
EUR 389

KoldPod, 1.5ml Micro Tube

0-120-0038 1.5 ml
EUR 152
Description: Thermo conductive tube pods

KoldPod, 15ml Conical Tube

0-120-0039 15 ml
EUR 275
Description: Thermo conductive tube pods

KoldPod, 50ml Conical Tube

0-120-0040 50 ml
EUR 290
Description: Thermo conductive tube pods

Model 150 V/T Ultrasonic Homogenizer

0-121-0002 210-240V/50-60Hz
EUR 2920
Description: Delivers up to 150 Watts of ultrasonic power to the Titanium Tip. The Timer and Duty Cycle function increase preciosion in sample processing processing.

Model 300 V/T Ultrasonic Homogenizer

0-122-0002 210-240V/50-60Hz
EUR 3520
Description: Delivers up to 300 Watts of ultrasonic power to the Titanium Tip. The Timer and Duty Cycle function increase preciosion in sample.

SONABOZ Sound Abating Chamber

0-125-0001 each
EUR 1020
Description: Reduces cavitational sound emitted during processing.

Model 3000 Ultrasonic Homogenizer

0-127-0002 210-240V/50-60Hz
EUR 4120
Description: Delivers up to 300 Watts of ultrasonic power to the Titanium Tip and includes an intergrated Sound Abating Chmaber to reduce cavitational sound emitted during processing. The Timer and Duty Cycle function increase preciosion in sample.

Model 3000MP Ultrasonic Homogenizer

0-128-0002 210-240V/50-60Hz
EUR 4720
Description: Delivers up to 300 Watts of ultrasonic power to the Titanium Tip with preciosion control from a microprocessor and a graphical user interface displayed on a large (145 mm) LCD display. The integrated Sound Abating Chamber reduces cavitational sound emitted during processing.

OMNICON® Zone Reader, 210-240V/50-60Hz

0-131-0002 210-240V/50-60Hz
EUR 35200
Description: Designed to Perform multi-plate Assays on round 90/100mm Petri Dishes. The integrated LED illumination system provides transmitted light for brightfield and darkfield illumination of transparent media.

OMNI-Noculator Peni Cylinder Filler, 210-240V/50-60Hz

0-134-0002 210-240V/50-60Hz
EUR 32200
Description: A robotic liquid handling system designed to dispense Peni Cylinders and fill Peni Cylinders with the corresponding antibiotic liquid sample.

Peni Cylinder Dispenser with Manual Hopper

0-144-0002 each
EUR 5406
Description: Dispenser can be configured to dispense 4 or 6 Peni Cylinders onto a petri dish.

Peni Cylinder Dispenser with Motorized Hopper, 100-240V/50-60Hz

0-144-0003 100-240V/50-60Hz
EUR 6254
Description: The motorized hopper can be configured to dispense 4 or 6 Peni Cylinders onto a petri Dispenser can be disassembled for disinfection.

Stainless Steel Peni Cylinder with Flat Face

0-144-0005 6mm I.D. x 8mm O.D. x 10mm Long
EUR 399
Description: sold in packages of 100 pieces

Stainless Steel Peni Cylinder with Chamfered Face

0-144-0006 6mm I.D. x 8mm O.D. x 10mm Long
EUR 412
Description: sold in packages of 100 pieces

Custom development of ELISAs for other species or antibody isotypes not listed in the catalog. Custom testing of samples for IgG/IgM/IgA or total (IgG+IgM+IgA)

000-CUS Custom
EUR 602

Alpha-Bungarotoxin, CF®405S, 500 ug

00002 1UG
EUR 527
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus)

Alpha-bungarotoxin, CF405s

00002 500uG
EUR 594
Description: Minimum order quantity: 1 unit of 500uG

Alpha-Bungarotoxin, CF®405S, 500 ug

00002-1 EA
EUR 527

Alpha-Bungarotoxin, CF®405S 100ug

00002-100ug 100uG
EUR 132
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus)

Alpha-Bungarotoxin, CF®680R, 500 ug

9-00003
  • Ask for price
  • Ask for price
  • 500uG
  • 100uG
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus)

Alpha-Bungarotoxin, CF®680R, 500 ug

00003-1 EA
EUR 527

Alpha-Bungarotoxin, CF®680R 100ug

9-00003
  • Ask for price
  • Ask for price
  • 500uG
  • 100uG
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus)

Alpha-Bungarotoxin, CF®640R, 500 ug

9-00004
  • Ask for price
  • Ask for price
  • 500uG
  • 100uG
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus)

Alpha-Bungarotoxin, CF®640R, 500 ug

00004-1 EA
EUR 527

Alpha-Bungarotoxin, CF®640R 100ug

9-00004
  • Ask for price
  • Ask for price
  • 500uG
  • 100uG
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus)

Alpha-Bungarotoxin, CF®488A, 500 ug

9-00005
  • Ask for price
  • Ask for price
  • 500uG
  • 100uG
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus)

Alpha-Bungarotoxin, CF®488A, 500 ug

00005-1 EA
EUR 527

Alpha-Bungarotoxin CF®488A 100ug

9-00005
  • Ask for price
  • Ask for price
  • 500uG
  • 100uG
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus)

Alpha-Bungarotoxin, CF®568, 500 ug

9-00006
  • Ask for price
  • Ask for price
  • 500uG
  • 100uG
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus)

Alpha-Bungarotoxin, CF®568, 500 ug

00006-1 EA
EUR 527

Alpha-Bungarotoxin, CF®568 100ug

9-00006
  • Ask for price
  • Ask for price
  • 500uG
  • 100uG
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus)

Alpha-Bungarotoxin, CF®594, 500 ug

9-00007
  • Ask for price
  • Ask for price
  • 500uG
  • 100uG
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus)

Alpha-Bungarotoxin, CF®594, 500 ug

00007-1 EA
EUR 527

Alpha-Bungarotoxin CF®594 100ug

9-00007
  • Ask for price
  • Ask for price
  • 500uG
  • 100uG
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus)

Alpha-Bungarotoxin, CF®633, 500 ug

9-00009
  • Ask for price
  • Ask for price
  • 500uG
  • 100uG
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus)

Alpha-Bungarotoxin, CF®633, 500 ug

00009-1 EA
EUR 527

Alpha-Bungarotoxin, CF®633 100ug

9-00009
  • Ask for price
  • Ask for price
  • 500uG
  • 100uG
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus)

SARS-CoV-2 Indicator Cell Line for RNA Replication - GFP Reporter only

0001-PP-001 1 cell line (can order x amount)
EUR 12000
Description: SARS-CoV-2 GFP reporter cell line using HEK293T (ACE2/TMPRSS2) cells

Alpha-Bungarotoxin, 1 mg

00010-1 1MG
EUR 193
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus)

Alpha-Bungarotoxin, 1 mg

00010-1-1 EA
EUR 193

Fluorescein-Alpha-Bungarotoxin, 500 ug

00011 500uG
EUR 376
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus)

Fluorescein-Alpha-Bungarotoxin, 500 ug

00011-1 EA
EUR 376

Tetramethylrhodamine-Alpha-Bungarotoxin, 500 ug

00012 500uG
EUR 394
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus)

Tetramethylrhodamine-Alpha-Bungarotoxin, 500 ug

00012-1 EA
EUR 394

Fluorescein-alpha-bungarotoxin, 10x50ug

00013 10ST
EUR 436
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus)

Fluorescein-alpha-bungarotoxin, 10x50ug

00013-1 EA
EUR 436

Tetramethylrhodamine-A-Bungarotoxin, 10x50 ug

00014 10ST
EUR 494
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus)

Tetramethylrhodamine-A-Bungarotoxin, 10x50 ug

00014-1 EA
EUR 494

Sulforhodamine 101-Alpha-Bungarotoxin, 500 ug

00015 500uG
EUR 494
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus)

Sulforhodamine 101-Alpha-Bungarotoxin, 500 ug

00015-1 EA
EUR 494

Sulforhodamine 101-Alpha-Bungarotoxin, 50 ug

00016 10ST
EUR 560
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus)

Sulforhodamine 101-Alpha-Bungarotoxin, 50 ug

00016-1 EA
EUR 560

Biotin-XX-A-Bungarotoxin, 500 ug

00017 500uG
EUR 455
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus)

Biotin-XX-A-Bungarotoxin, 500 ug

00017-1 EA
EUR 455

Alpha-Bungarotoxin, CF®555, 500 ug

9-00018
  • Ask for price
  • Ask for price
  • 500uG
  • 100ug
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus)

Alpha-Bungarotoxin, CF®555, 500 ug

00018-1 EA
EUR 527

Alpha-Bungarotoxin, CF®555 100ug

9-00018
  • Ask for price
  • Ask for price
  • 500uG
  • 100ug
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus)

Acrylamide, Chemzymes Ultra Pure®

00019-100 100g
EUR 101.52
Description: 79-06-1

Acrylamide, Chemzymes Ultra Pure®

00019-500 500g
EUR 270
Description: 79-06-1

Biotin-cAMP, 1 mg

00020 1MG
EUR 298
Description: N/A

Biotin-cAMP, 1 mg

00020-1 20ST
EUR 298
Description: N/A

Biotin-cAMP, 50 ug

00020-1-1 EA
EUR 414

Biotin-cGMP, 1 mg

00021 1MG
EUR 331
Description: N/A

Biotin-cGMP, 1 mg

00021-1 20ST
EUR 331
Description: N/A

Biotin-cGMP, 20x50 ug

00021-1-1 EA
EUR 447

Cyanine 644-cAMP, 1 mg

00022 1MG
EUR 496
Description: N/A

Cyanine 644-cAMP, 1 mg

00022-1 20ST
EUR 496
Description: N/A