DNA recovery from Droplet Digital PCR emulsions using liquid nitrogen

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Analysis of the Molecular Diversity Among Cronobacter Species Isolated From Filth Flies Using Targeted PCR, Pan Genomic DNA Microarray, and Whole Genome Sequencing Analyses

 

Cronobacter species are opportunistic pathogens capable of causing life-threatening infections in humans, with serious complications arising in neonates, infants, immuno-compromised individuals, and elderly adults. The genus is comprised of seven species: Cronobacter sakazakii, Cronobacter malonaticus, Cronobacter turicensis, Cronobacter muytjensii, Cronobacter dublinensis, Cronobacter universalis, and Cronobacter condimenti. Despite a multiplicity of genomic data for the genus, little is known about likely transmission vectors. Using DNA microarray analysis, in parallel with whole genome sequencing, and targeted PCR analyses, the total gene content of two C. malonaticus, three C. turicensis, and 14 C. sakazaki isolated from various filth flies was assessed.
Phylogenetic relatedness among these and other strains obtained during surveillance and outbreak investigations were comparatively assessed. Specifically, microarray analysis (MA) demonstrated its utility to cluster strains according to species-specific and sequence type (ST) phylogenetic relatedness, and that the fly strains clustered among strains obtained from clinical, food and environmental sources from United States, Europe, and Southeast Asia. This combinatorial approach was useful in data mining for virulence factor genes, and phage genes and gene clusters. In addition, results of plasmidotyping were in agreement with the species identity for each strain as determined by species-specific PCR assays, MA, and whole genome sequencing.
Microarray and BLAST analyses of Cronobacter fly sequence datasets were corroborative and showed that the presence and absence of virulence factors followed species and ST evolutionary lines even though such genes were orthologous. Additionally, zebrafish infectivity studies showed that these pathotypes were as virulent to zebrafish embryos as other clinical strains. In summary, these findings support a striking phylogeny amongst fly, clinical, and surveillance strains isolated during 2010-2015, suggesting that flies are capable vectors for transmission of virulent Cronobacter spp.; they continue to circulate among United States and European populations, environments, and that this “pattern of circulation” has continued over decades.

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HCV10009 6 X 1 mL
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HCV10010 7 X 1 mL
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HCV Seroconversion Panel Donor# 66666 (13 X 1 mL)

HCV10011 13 X 1 mL
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HCV Seroconversion Panel Donor# 66747 (13 X 1 mL)

HCV10012 13 X 1 mL
EUR 986
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HCV Seroconversion Panel Donor# 67356 (10 X 1 mL)

HCV10013 10 X 1 mL
EUR 986
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HCV Seroconversion Panel Donor# 68157 (4 X 1 mL)

HCV10014 4 X 1 mL
EUR 986
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HCV Seroconversion Panel Donor# 68628 (12 X 1 mL)

HCV10016 12 X 1 mL
EUR 986
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HCV Seroconversion Panel Donor# 65064 (16 X 1 mL)

HCV10017 16 X 1 mL
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HCV Seroconversion Panel Donor# 69142 (8 X 1 mL)

HCV10019 8 X 1 mL
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HCV Seroconversion Panel Donor# 68744 (13 X 1 mL)

HCV10020 13 X 1 mL
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HCV Seroconversion Panel Donor# 68470 (14 X 1 mL)

HCV10021 14 X 1 mL
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HCV Seroconversion Panel Donor# 68450 (11 X 1 mL)

HCV10022 11 X 1 mL
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HCV Seroconversion Panel Donor# 67505 (21 X 1 mL)

HCV10023 21 X 1 mL
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HCV Seroconversion Panel Donor# 66719 (8 X 1 mL)

HCV10024 8 X 1 mL
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HCV Seroconversion Panel Donor# 66801 (11 X 1 mL)

HCV10025 11 X 1 mL
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HCV Seroconversion Panel Donor# 67391 (12 X 1 mL)

HCV10026 12 X 1 mL
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HCV Seroconversion Panel Donor# 66751 (8 X 1 mL)

HCV10027 8 X 1 mL
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HCV Seroconversion Panel Donor# 67116 (7 X 1 mL)

HCV10028 7 X 1 mL
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HCV Seroconversion Panel Donor# 67539 (16 X 1 mL)

HCV10029 16 X 1 mL
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HCV Seroconversion Panel Donor# 65977 (8 X 1 mL)

HCV10030 8 X 1 mL
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HCV Seroconversion Panel Donor# 66113 (16 X 1 mL)

HCV10032 16 X 1 mL
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HCV Seroconversion Panel Donor# 68629 (10 X 1 mL)

HCV10033 10 X 1 mL
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HCV Seroconversion Panel Donor# 68223 (13 X 1 mL)

HCV10036 13 X 1 mL
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HCV Seroconversion Panel Donor# 65643 (10 X 1 mL)

HCV10043 10 X 1 mL
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HCV Seroconversion Panel Donor# 65982 (4 X 1 mL)

HCV10044 4 X 1 mL
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HCV Seroconversion Panel Donor# 66074 (5 X 1 mL)

HCV10045 5 X 1 mL
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HCV Seroconversion Panel Donor# 67766 (15 X 1 mL)

HCV10050 15 X 1 mL
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HCV Seroconversion Panel Donor# 67787 (14 X 1 mL)

HCV10051 14 X 1 mL
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HCV Seroconversion Panel Donor# 67861 (12 X 1 mL)

HCV10052 12 X 1 mL
EUR 986
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HCV Seroconversion Panel Donor# 420245049 (7 X 1 mL)

HCV10057 7 X 1 mL
EUR 1548.64
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HCV Seroconversion Panel Donor# 420299823 (7 X 1 mL)

HCV10058 7 X 1 mL
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HCV Seroconversion Panel Donor# 490104584 (8 X 1 mL)

HCV10062 8 X 1 mL
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HCV Seroconversion Panel Donor# 90163052 (7 X 1 mL)

HCV10071 7 X 1 mL
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HCV Seroconversion Panel Donor# 72237 (9 X 1 mL)

HCV10165 9 X 1 mL
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HCV Seroconversion Panel Donor# 70225 (5 X 1 mL)

HCV10185 5 X 1 mL
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HCV Seroconversion Panel Donor# 61025 (5 X 1 mL)

HCV6217 5 X 1 mL
EUR 928.8
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HCV6218 8 X 1 mL
EUR 928.8
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HCV6219 14 X 1 mL
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HCV Seroconversion Panel Donor# 63792 (6 X 1 mL)

HCV6220 6 X 1 mL
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HCV6221 8 X 1 mL
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HCV6222 8 X 1 mL
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HCV6223 22 X 1 mL
EUR 928.8
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HCV Seroconversion Panel Donor# 62680 (6 X 1 mL)

HCV6224 6 X 1 mL
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HCV Seroconversion Panel Donor# 62999 (19 X 1 mL)

HCV6225 19 X 1 mL
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HCV Seroconversion Panel Donor# 63135 (12 X 1 mL)

HCV6226 12 X 1 mL
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HCV Seroconversion Panel Donor# 63318 (7 X 1 mL)

HCV6227 7 X 1 mL
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HCV6228 12 X 1 mL
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HCV6229 8 X 1 mL
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HCV Seroconversion Panel Donor# 63625 (8 X 1 mL)

HCV9041 8 X 1 mL
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HCV Seroconversion Panel Donor# 63792 (6 X 1 mL)

HCV9042 6 X 1 mL
EUR 928.8
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HCV Seroconversion Panel Donor# 63869 (6 X 1 mL)

HCV9043 6 X 1 mL
EUR 928.8
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HCV Seroconversion Panel Donor# 63911 (6 X 1 mL)

HCV9044 6 X 1 mL
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HCV Seroconversion Panel Donor# 64150 (8 X 1 mL)

HCV9045 8 X 1 mL
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HCV9046 5 X 1 mL
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HCV Seroconversion Panel Donor# 64273 (10 X 1 mL)

HCV9047 10 X 1 mL
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HCV Seroconversion Panel Donor# 64437 (8 X 1 mL)

HCV9048 8 X 1 mL
EUR 928.8
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HCV9049 5 X 1 mL
EUR 928.8
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HCV9050 13 X 1 mL
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HCV9054 8 X 1 mL
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HCV9055 11 X 1 mL
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HCV9057 11 X 1 mL
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HCV9058 5 X 1 mL
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HCV9094 5 X 1 mL
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HCV9095 5 X 1 mL
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HCV10235 5 X 1 mL
EUR 512.8
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EBV9097 14 X 1 mL
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HBV10231 5 X 1 mL
EUR 582.48
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HBV10232 6 X 1 mL
EUR 582.48
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HBV11000 9 X 1 mL
EUR 859.12
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HBV11001 8 X 1 mL
EUR 859.12
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HBV11002 6 X 1 mL
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HBV11003 8 X 1 mL
EUR 859.12
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HBV11004 8 X 1 mL
EUR 859.12
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HBV11005 14 X 1 mL
EUR 859.12
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HBV11006 17 X 1 mL
EUR 859.12
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HBV11007 14 X 1 mL
EUR 859.12
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HBV11008 18 X 1 mL
EUR 859.12
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HBV11009 23 X 1 mL
EUR 859.12
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HBV11010 18 X 1 mL
EUR 859.12
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HBV11011 14 X 1 mL
EUR 859.12
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HBV Seroconversion Panel Donor# 67773 (6 X 1 mL)

HBV11012 6 X 1 mL
EUR 859.12
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HBV Seroconversion Panel Donor# 67962 (35 X 1 mL)

HBV11013 35 X 1 mL
EUR 859.12
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HBV11014 12 X 1 mL
EUR 859.12
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HBV11015 14 X 1 mL
EUR 859.12
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HBV11016 10 X 1 mL
EUR 859.12
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HBV11017 14 X 1 mL
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HBV Seroconversion Panel Donor# 70793 (14 X 1 mL)

HBV11024 14 X 1 mL
EUR 859.12
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HBV11026 16 X 1 mL
EUR 859.12
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HBV11027 13 X 1 mL
EUR 859.12
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HBV11028 10 X 1 mL
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HBV11029 13 X 1 mL
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HBV11031 15 X 1 mL
EUR 859.12
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HBV Seroconversion Panel Donor# 72439 (13 X 1 mL)

HBV11052 13 X 1 mL
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DNA recovery from Droplet Digital PCR emulsions using liquid nitrogen

 

 

Droplet microfluidics is a technology that enables the production and manipulation of small volumes. In biosciences, the most popular application of this technology is Droplet Digital™ PCR (ddPCR™), where parallel nanoliter-scale PCR assays are used to provide a high sensitivity and specificity for DNA detection.

 

However, the recovery of PCR products for downstream applications such as sequencing can be challenging due to the droplets’ stability. Here we compared five methods for disrupting the droplets to recover DNA. We found that rapid freezing in liquid nitrogen results in a clear phase separation and recovery of up to 70% of the DNA content. Liquid nitrogen freezing can thus offer a simple and environmentally friendly protocol for recovering DNA from ddPCR.

 

Classification and review of free PCR primer design software

 

Motivation: Polymerase chain reaction (PCR) has been a revolutionary biomedical advancement. However, for PCR to be appropriately used, one must spend a significant amount of effort on PCR primer design. Carefully designed PCR primers not only increase sensitivity and specificity, but also decrease effort spent on experimental optimization. Computer software removes the human element by performing and automating the complex and rigorous calculations required in PCR primer design. Classification and review of the available software options and their capabilities should be a valuable resource for any PCR application.
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Results: This paper focuses on currently available free PCR primer design software and their major functions (https://pcrprimerdesign.github.io/). The software are classified according to their PCR applications, such as Sanger sequencing, reverse transcription quantitative PCR, single nucleotide polymorphism detection, splicing variant detection, methylation detection, microsatellite detection, multiplex PCR and targeted next generation sequencing, and conserved/degenerate primers to clone orthologous genes from related species, new gene family members in the same species, or to detect a group of related pathogens. Each software is summarized to provide a technical review of their capabilities and utilities.

Sensitive detection and quantification of SARS-CoV-2 by multiplex droplet digital RT-PCR

 

The purpose of this study is to develop a one-step droplet digital RT-PCR (RT-ddPCR) multiplex assay that allows for sensitive quantification of SARS-CoV-2 RNA with respect to human-derived RNA and could be used for screening and monitoring of Covid-19 patients. A one-step RT-ddPCR multiplex assay was developed for simultaneous detection of SARS-CoV-2 E, RdRp and N viral RNA, and human Rpp30 DNA and GUSB mRNA, for internal nucleic acid (NA) extraction and RT-PCR control. Dilution series of viral RNA transcripts were prepared in water and total NA extract of Covid-19-negative patients. As reference assay, an E-GUSB duplex RT-PCR was used.

 

GUSB mRNA detection was used to set validity criteria to assure viral RNA and RT-PCR assay quality and to enable quantification of SARS-CoV-2 RNA. In a background of at least 100 GUSB mRNA copies, 5 copies of viral RNA are reliably detectable and 10 copies viral RNA copies are reliably quantifiable. It was found that assay sensitivity of the RT-ddPCR was not affected by the total NA background while assay sensitivity of the gold standard RT-PCR assay is drastically decreased when SARS-CoV-2 copies were detected in a background of total NA extract compared with water. The present study describes a robust and sensitive one-step ddRT-PCR multiplex assay for reliable quantification of SARS-CoV-2 RNA. By determining the fractional abundance of viral RNA with respect to a human housekeeping gene, viral loads from different samples can be compared, what could be used to investigate the infectiveness and to monitor Covid-19 patients.

 

rRT-PCR Results of a Covid-19 Diagnosed Geriatric Patient

 

In this study, we aimed to present a geriatric patient with the diagnosis of COVID-19 and with contradictory results in rRT-PCR examinations in short time intervals. A 69-year-old male patient was admitted to the emergency room on the 18th day of May 2020, with the complaints of fever, sweating, myalgia, dry cough that continued for 5 days, and the lack of taste that started on the day he applied to the emergency room. Comorbidity factors include diabetes mellitus, bronchial asthma, and hypertension. The patient has a history of 36 years of smoking 1.5 packs per day. High laboratory findings during hospitalization: monocytes, creatinine, CRP (C-reactive protein). In the thorax CT, in the parenchyma areas of both lungs, there are increases in attenuation with multilobe distributions (more visible at the level of the upper lobes) in the form of ground-glass opacities.

 

May 19, 2020, was subjected to the rRT-PCR test, repeated twice on the 19th of May which also resulted in positive. Despite rRT-PCR tests, which were negative on 27th of May and positive on 28th of May, the patient, whose symptoms disappeared, and general condition improved, was discharged on June 1, 2020, with the recommendation for home isolation. In our case, unlike the incubation period only, we encountered a negative rRT-PCR result on the 8th day after diagnosis. Therefore, the COVID-19 pandemic control and filiation evaluation with the rRT-PCR test may produce false negative results.

 

HCV Seroconversion Panel Donor# 68896 (6 X 1 mL)

HCV10009 6 X 1 mL
EUR 986
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HCV Seroconversion Panel Donor# 65470 (7 X 1 mL)

HCV10010 7 X 1 mL
EUR 986
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HCV Seroconversion Panel Donor# 66666 (13 X 1 mL)

HCV10011 13 X 1 mL
EUR 986
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HCV Seroconversion Panel Donor# 66747 (13 X 1 mL)

HCV10012 13 X 1 mL
EUR 986
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HCV Seroconversion Panel Donor# 67356 (10 X 1 mL)

HCV10013 10 X 1 mL
EUR 986
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HCV Seroconversion Panel Donor# 68157 (4 X 1 mL)

HCV10014 4 X 1 mL
EUR 986
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HCV Seroconversion Panel Donor# 68628 (12 X 1 mL)

HCV10016 12 X 1 mL
EUR 986
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HCV Seroconversion Panel Donor# 65064 (16 X 1 mL)

HCV10017 16 X 1 mL
EUR 1548.64
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HCV Seroconversion Panel Donor# 69142 (8 X 1 mL)

HCV10019 8 X 1 mL
EUR 986
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HCV Seroconversion Panel Donor# 68744 (13 X 1 mL)

HCV10020 13 X 1 mL
EUR 986
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HCV Seroconversion Panel Donor# 68470 (14 X 1 mL)

HCV10021 14 X 1 mL
EUR 986
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HCV Seroconversion Panel Donor# 68450 (11 X 1 mL)

HCV10022 11 X 1 mL
EUR 986
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HCV Seroconversion Panel Donor# 67505 (21 X 1 mL)

HCV10023 21 X 1 mL
EUR 986
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HCV Seroconversion Panel Donor# 66719 (8 X 1 mL)

HCV10024 8 X 1 mL
EUR 986
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HCV Seroconversion Panel Donor# 66801 (11 X 1 mL)

HCV10025 11 X 1 mL
EUR 986
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HCV Seroconversion Panel Donor# 67391 (12 X 1 mL)

HCV10026 12 X 1 mL
EUR 1548.64
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HCV Seroconversion Panel Donor# 66751 (8 X 1 mL)

HCV10027 8 X 1 mL
EUR 986
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HCV Seroconversion Panel Donor# 67116 (7 X 1 mL)

HCV10028 7 X 1 mL
EUR 986
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HCV Seroconversion Panel Donor# 67539 (16 X 1 mL)

HCV10029 16 X 1 mL
EUR 986
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HCV Seroconversion Panel Donor# 65977 (8 X 1 mL)

HCV10030 8 X 1 mL
EUR 986
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HCV Seroconversion Panel Donor# 66113 (16 X 1 mL)

HCV10032 16 X 1 mL
EUR 986
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HCV Seroconversion Panel Donor# 68629 (10 X 1 mL)

HCV10033 10 X 1 mL
EUR 986
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HCV Seroconversion Panel Donor# 68223 (13 X 1 mL)

HCV10036 13 X 1 mL
EUR 986
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HCV Seroconversion Panel Donor# 65643 (10 X 1 mL)

HCV10043 10 X 1 mL
EUR 986
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 65982 (4 X 1 mL)

HCV10044 4 X 1 mL
EUR 986
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 66074 (5 X 1 mL)

HCV10045 5 X 1 mL
EUR 986
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 67766 (15 X 1 mL)

HCV10050 15 X 1 mL
EUR 986
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 67787 (14 X 1 mL)

HCV10051 14 X 1 mL
EUR 986
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 67861 (12 X 1 mL)

HCV10052 12 X 1 mL
EUR 986
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 420245049 (7 X 1 mL)

HCV10057 7 X 1 mL
EUR 1548.64
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 420299823 (7 X 1 mL)

HCV10058 7 X 1 mL
EUR 1548.64
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 490104584 (8 X 1 mL)

HCV10062 8 X 1 mL
EUR 1548.64
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 90163052 (7 X 1 mL)

HCV10071 7 X 1 mL
EUR 1548.64
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 72237 (9 X 1 mL)

HCV10165 9 X 1 mL
EUR 1548.64
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 70225 (5 X 1 mL)

HCV10185 5 X 1 mL
EUR 1548.64
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 61025 (5 X 1 mL)

HCV6217 5 X 1 mL
EUR 928.8
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 61251 (8 X 1 mL)

HCV6218 8 X 1 mL
EUR 928.8
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 60749 (14 X 1 mL)

HCV6219 14 X 1 mL
EUR 928.8
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 63792 (6 X 1 mL)

HCV6220 6 X 1 mL
EUR 928.8
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 62258 (8 X 1 mL)

HCV6221 8 X 1 mL
EUR 928.8
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 62286 (8 X 1 mL)

HCV6222 8 X 1 mL
EUR 1548.64
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 62507 (22 X 1 mL)

HCV6223 22 X 1 mL
EUR 928.8
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 62680 (6 X 1 mL)

HCV6224 6 X 1 mL
EUR 1548.64
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 62999 (19 X 1 mL)

HCV6225 19 X 1 mL
EUR 1548.64
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 63135 (12 X 1 mL)

HCV6226 12 X 1 mL
EUR 1548.64
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 63318 (7 X 1 mL)

HCV6227 7 X 1 mL
EUR 1548.64
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 62886 (12 X 1 mL)

HCV6228 12 X 1 mL
EUR 1548.64
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 62804 (8 X 1 mL)

HCV6229 8 X 1 mL
EUR 1548.64
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 63625 (8 X 1 mL)

HCV9041 8 X 1 mL
EUR 1548.64
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 63792 (6 X 1 mL)

HCV9042 6 X 1 mL
EUR 928.8
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 63869 (6 X 1 mL)

HCV9043 6 X 1 mL
EUR 928.8
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 63911 (6 X 1 mL)

HCV9044 6 X 1 mL
EUR 1548.64
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 64150 (8 X 1 mL)

HCV9045 8 X 1 mL
EUR 1548.64
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 64254 (5 X 1 mL)

HCV9046 5 X 1 mL
EUR 1548.64
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 64273 (10 X 1 mL)

HCV9047 10 X 1 mL
EUR 1548.64
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 64437 (8 X 1 mL)

HCV9048 8 X 1 mL
EUR 928.8
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 64766 (5 X 1 mL)

HCV9049 5 X 1 mL
EUR 928.8
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 66158 (13 X 1 mL)

HCV9050 13 X 1 mL
EUR 1548.64
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 66626 (8 X 1 mL)

HCV9054 8 X 1 mL
EUR 1548.64
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 66732 (11 X 1 mL)

HCV9055 11 X 1 mL
EUR 1548.64
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 66861 (11 X 1 mL)

HCV9057 11 X 1 mL
EUR 1548.64
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 66647 (5 X 1 mL)

HCV9058 5 X 1 mL
EUR 1548.64
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 22708D (5 X 1 mL)

HCV9094 5 X 1 mL
EUR 1548.64
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 1038851 (5 X 1 mL)

HCV9095 5 X 1 mL
EUR 1548.64
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Pre/Post Seroconversion Panel Donor# 66822 (5 X 1 mL)

HCV10235 5 X 1 mL
EUR 512.8
Description: Please contact Gentaur in order to receive the datasheet of the product.

HIV Seroconversion Panel Donor# 65404 (7 X mL)

HIV9013 7 X mL
EUR 1548.64
Description: Please contact Gentaur in order to receive the datasheet of the product.

EBV Seroconversion Panel Donor# 283843421 (14 X 1 mL)

EBV9097 14 X 1 mL
EUR 1548.64
Description: Please contact Gentaur in order to receive the datasheet of the product.

HBV Seroconversion Panel Donor# 64090 (5 X 1 mL)

HBV10231 5 X 1 mL
EUR 582.48
Description: Please contact Gentaur in order to receive the datasheet of the product.

HBV Seroconversion Panel Donor# 64017 (6 X 1 mL)

HBV10232 6 X 1 mL
EUR 582.48
Description: Please contact Gentaur in order to receive the datasheet of the product.

HBV Seroconversion Panel Donor# 65449 (9 X 1 mL)

HBV11000 9 X 1 mL
EUR 859.12
Description: Please contact Gentaur in order to receive the datasheet of the product.

HBV Seroconversion Panel Donor# 65524 (8 X 1 mL)

HBV11001 8 X 1 mL
EUR 859.12
Description: Please contact Gentaur in order to receive the datasheet of the product.

HBV Seroconversion Panel Donor# 65556 (6 X 1 mL)

HBV11002 6 X 1 mL
EUR 859.12
Description: Please contact Gentaur in order to receive the datasheet of the product.

HBV Seroconversion Panel Donor# 65584 (8 X 1 mL)

HBV11003 8 X 1 mL
EUR 859.12
Description: Please contact Gentaur in order to receive the datasheet of the product.

HBV Seroconversion Panel Donor# 65732 (8 X 1 mL)

HBV11004 8 X 1 mL
EUR 859.12
Description: Please contact Gentaur in order to receive the datasheet of the product.

HBV Seroconversion Panel Donor# 65777 (14 X 1 mL)

HBV11005 14 X 1 mL
EUR 859.12
Description: Please contact Gentaur in order to receive the datasheet of the product.

HBV Seroconversion Panel Donor# 66201 (17 X 1 mL)

HBV11006 17 X 1 mL
EUR 859.12
Description: Please contact Gentaur in order to receive the datasheet of the product.

HBV Seroconversion Panel Donor# 66537 (14 X 1 mL)

HBV11007 14 X 1 mL
EUR 859.12
Description: Please contact Gentaur in order to receive the datasheet of the product.

HBV Seroconversion Panel Donor# 67303 (18 X 1 mL)

HBV11008 18 X 1 mL
EUR 859.12
Description: Please contact Gentaur in order to receive the datasheet of the product.

HBV Seroconversion Panel Donor# 67449 (23 X 1 mL)

HBV11009 23 X 1 mL
EUR 859.12
Description: Please contact Gentaur in order to receive the datasheet of the product.

HBV Seroconversion Panel Donor# 67457 (18 X 1 mL)

HBV11010 18 X 1 mL
EUR 859.12
Description: Please contact Gentaur in order to receive the datasheet of the product.

HBV Seroconversion Panel Donor# 67694 (14 X 1 mL)

HBV11011 14 X 1 mL
EUR 859.12
Description: Please contact Gentaur in order to receive the datasheet of the product.

HBV Seroconversion Panel Donor# 67773 (6 X 1 mL)

HBV11012 6 X 1 mL
EUR 859.12
Description: Please contact Gentaur in order to receive the datasheet of the product.

HBV Seroconversion Panel Donor# 67962 (35 X 1 mL)

HBV11013 35 X 1 mL
EUR 859.12
Description: Please contact Gentaur in order to receive the datasheet of the product.

HBV Seroconversion Panel Donor# 68029 (12 X 1 mL)

HBV11014 12 X 1 mL
EUR 859.12
Description: Please contact Gentaur in order to receive the datasheet of the product.

HBV Seroconversion Panel Donor# 68105 (14 X 1 mL)

HBV11015 14 X 1 mL
EUR 859.12
Description: Please contact Gentaur in order to receive the datasheet of the product.

HBV Seroconversion Panel Donor# 68541 (10 X 1 mL)

HBV11016 10 X 1 mL
EUR 859.12
Description: Please contact Gentaur in order to receive the datasheet of the product.

HBV Seroconversion Panel Donor# 68739 (14 X 1 mL)

HBV11017 14 X 1 mL
EUR 859.12
Description: Please contact Gentaur in order to receive the datasheet of the product.

HBV Seroconversion Panel Donor# 70793 (14 X 1 mL)

HBV11024 14 X 1 mL
EUR 859.12
Description: Please contact Gentaur in order to receive the datasheet of the product.

HBV Seroconversion Panel Donor# 70970 (16 X 1 mL)

HBV11026 16 X 1 mL
EUR 859.12
Description: Please contact Gentaur in order to receive the datasheet of the product.

HBV Seroconversion Panel Donor# 71415 (13 X 1 mL)

HBV11027 13 X 1 mL
EUR 859.12
Description: Please contact Gentaur in order to receive the datasheet of the product.

HBV Seroconversion Panel Donor# 71840 (10 X 1 mL)

HBV11028 10 X 1 mL
EUR 859.12
Description: Please contact Gentaur in order to receive the datasheet of the product.

HBV Seroconversion Panel Donor# 69751 (13 X 1 mL)

HBV11029 13 X 1 mL
EUR 859.12
Description: Please contact Gentaur in order to receive the datasheet of the product.

HBV Seroconversion Panel Donor# 70292 (15 X 1 mL)

HBV11031 15 X 1 mL
EUR 859.12
Description: Please contact Gentaur in order to receive the datasheet of the product.

HBV Seroconversion Panel Donor# 72439 (13 X 1 mL)

HBV11052 13 X 1 mL
EUR 859.12
Description: Please contact Gentaur in order to receive the datasheet of the product.

 

Alpha-bungarotoxin, CF568

9-00006
  • EUR 494.00
  • EUR 161.00
  • 500uG
  • 100uG
Description: Minimum order quantity: 1 unit of 100uG

Alpha-bungarotoxin, CF594

9-00007
  • EUR 494.00
  • EUR 161.00
  • 500uG
  • 100uG
Description: Minimum order quantity: 1 unit of 500uG

Alpha-bungarotoxin CF594

9-00007
  • EUR 494.00
  • EUR 161.00
  • 500uG
  • 100uG
Description: Minimum order quantity: 1 unit of 100uG

Alpha-bungarotoxin, CF633

9-00009
  • EUR 494.00
  • EUR 161.00
  • 500uG
  • 100uG
Description: Minimum order quantity: 1 unit of 500uG

Alpha-bungarotoxin, CF633

9-00009
  • EUR 494.00
  • EUR 161.00
  • 500uG
  • 100uG
Description: Minimum order quantity: 1 unit of 100uG

Alpha-bungarotoxin

00010-1 1MG
EUR 168
Description: Minimum order quantity: 1 unit of 1MG

Fluorescein-Alpha-bungarotoxin

00011 500uG
EUR 367
Description: Minimum order quantity: 1 unit of 500uG

Tetramethylrhodamine-Alpha-bungarotoxin

00012 500uG
EUR 383
Description: Minimum order quantity: 1 unit of 500uG

Fluorescein-alpha-bungarotoxin: (10x50ug)

00013 10ST
EUR 417
Description: Minimum order quantity: 1 unit of 10ST

Tetramethylrhodamine-alpha-bungarotoxin: (10x50ug)

00014 10ST
EUR 466
Description: Minimum order quantity: 1 unit of 10ST

Sulforhodamine 101-a-bungarotoxin (Texas Red--a-bungarotoxin): (500ug)

00015 500uG
EUR 466
Description: Minimum order quantity: 1 unit of 500uG

Sulforhodamine 101-a-bungarotoxin (Texas Red--a-bungarotoxin): (10x50ug)

00016 10ST
EUR 523
Description: Minimum order quantity: 1 unit of 10ST

Biotin-xx-a-bungarotoxin

00017 500uG
EUR 433
Description: Minimum order quantity: 1 unit of 500uG

Alpha-Bungarotoxin, CF555 conjugate

9-00018
  • EUR 494.00
  • EUR 161.00
  • 500uG
  • 100ug
Description: Minimum order quantity: 1 unit of 500uG

Alpha-Bungarotoxin, CF555 conjugate

9-00018
  • EUR 494.00
  • EUR 161.00
  • 500uG
  • 100ug
Description: Minimum order quantity: 1 unit of 100ug

Biotin-cAMP, diisopropylethylammonium salt:

00020 1MG
EUR 314
Description: Minimum order quantity: 1 unit of 1MG

Biotin-cAMP, diisopropylethylammonium salt: (20x50ug)

00020-1 20ST
EUR 418
Description: Minimum order quantity: 1 unit of 20ST

Biotin-cGMP, diisopropylethylammonium salt:

00021 1MG
EUR 344
Description: Minimum order quantity: 1 unit of 1MG

Biotin-cGMP, diisopropylethylammonium salt: (20x50ug)

00021-1 20ST
EUR 448
Description: Minimum order quantity: 1 unit of 20ST

Cyanine 644-cAMP:

00022 1MG
EUR 491
Description: Minimum order quantity: 1 unit of 1MG

Cyanine 644-cAMP: (20x50ug)

00022-1 20ST
EUR 625
Description: Minimum order quantity: 1 unit of 20ST

Fluorescein Methotrexate, triammonium salt:

00023 1MG
EUR 281
Description: Minimum order quantity: 1 unit of 1MG

Staurosporine

00025 100uG
EUR 139
Description: Minimum order quantity: 1 unit of 100uG

Alpha-bungarotoxin, CF543

00026 500uG
EUR 494
Description: Minimum order quantity: 1 unit of 500uG

Rhodamine phalloidin 300u

00027 300
EUR 345
Description: Minimum order quantity: 1 unit of 300

Biotin-xx-phalloidin

00028 100U
EUR 428
Description: Minimum order quantity: 1 unit of 100U

Fluorescein-phalloidin

00030 300U
EUR 345
Description: Minimum order quantity: 1 unit of 300U

Rhodamine 110 phalloidin

00032 300ST
EUR 345
Description: Minimum order quantity: 1 unit of 300ST

Sulforhodamine 101 (Texas-Red) Phalloidin

00033 300EU
EUR 345
Description: Minimum order quantity: 1 unit of 300EU

Phalloidin, CF405M

00034 300U
EUR 442
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF405M

00034-T 50U
EUR 133
Description: Minimum order quantity: 1 unit of 50U

CF488A-cAMP

00036 100ug
EUR 172
Description: Minimum order quantity: 1 unit of 100ug

CF640R-cAMP

00037 100ug
EUR 172
Description: Minimum order quantity: 1 unit of 100ug

Phalloidin, CF555

00040 300U
EUR 442
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF555

00040-T 50U
EUR 133
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF647

00041 300U
EUR 442
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF647

00041-T 50U
EUR 133
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF488A

00042 300U
EUR 442
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF488A

00042-T 50U
EUR 133
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF543

00043 300U
EUR 442
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF543

00043-T 50U
EUR 133
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF568

00044 300U
EUR 442
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF568

00044-T 50U
EUR 133
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF594

00045 300U
EUR 442
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF594

00045-T 50U
EUR 133
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF633

00046 300U
EUR 442
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF633

00046-T 50U
EUR 133
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF660R

00047 300U
EUR 442
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF660R

00047-T 50U
EUR 133
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF680R

00048 300U
EUR 466
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF680R

00048-T 50U
EUR 133
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF350

00049 300U
EUR 442
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF350

00049-T 50U
EUR 133
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF640R

00050 300U
EUR 442
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF640R

00050-T 50U
EUR 133
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF532

00051 300U
EUR 442
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF532

00051-T 50U
EUR 133
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF660C

00052 300U
EUR 442
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF660C

00052-T 50U
EUR 133
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF680

00053 300U
EUR 466
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF680

00053-T 50U
EUR 147
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF430

00054 300U
EUR 449
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF430

00054-T 50U
EUR 139
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF440

00055 300U
EUR 449
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF440

00055-T 50U
EUR 139
Description: Minimum order quantity: 1 unit of 50U

Cholera Toxin Subunit B, CF488A conjugate

00070 100ug
EUR 261
Description: Minimum order quantity: 1 unit of 100ug

Cholera Toxin Subunit B, CF568 conjugate

00071 100ug
EUR 261
Description: Minimum order quantity: 1 unit of 100ug

Cholera Toxin Subunit B, CF594 conjugate

00072 100ug
EUR 261
Description: Minimum order quantity: 1 unit of 100ug

Cholera Toxin Subunit B, CF640R conjugate

00073 100ug
EUR 264
Description: Minimum order quantity: 1 unit of 100ug

Cholera Toxin Subunit B, CF532 conjugate

00074 100ug
EUR 264
Description: Minimum order quantity: 1 unit of 100ug

Cholera Toxin Subunit B, CF543 conjugate

00075 100ug
EUR 264
Description: Minimum order quantity: 1 unit of 100ug

Cholera Toxin Subunit B, CF620R conjugate

00076 100ug
EUR 264
Description: Minimum order quantity: 1 unit of 100ug

Cholera Toxin Subunit B, CF633 conjugate

00077 100ug
EUR 264
Description: Minimum order quantity: 1 unit of 100ug

Cholera Toxin Subunit B, CF660R conjugate

00078 100ug
EUR 264
Description: Minimum order quantity: 1 unit of 100ug

Cholera Toxin Subunit B, CF680R conjugate

00079 100ug
EUR 282
Description: Minimum order quantity: 1 unit of 100ug

Human Transferrin, CF488A conjugate

00081 1mg
EUR 149
Description: Minimum order quantity: 1 unit of 1mg

Human Transferrin, CF543 conjugate

00082 1mg
EUR 149
Description: Minimum order quantity: 1 unit of 1mg

Human Transferrin, CF568 conjugate

00083 1mg
EUR 149
Description: Minimum order quantity: 1 unit of 1mg

Human Transferrin, CF594 conjugate

00084 1mg
EUR 149
Description: Minimum order quantity: 1 unit of 1mg

Human Transferrin, CF640R conjugate

00085 1mg
EUR 149
Description: Minimum order quantity: 1 unit of 1mg