Analysis of the Molecular Diversity Among Cronobacter Species Isolated From Filth Flies Using Targeted PCR, Pan Genomic DNA Microarray, and Whole Genome Sequencing Analyses

 

DNA recovery from Droplet Digital PCR emulsions using liquid nitrogen

 

 

Droplet microfluidics is a technology that enables the production and manipulation of small volumes. In biosciences, the most popular application of this technology is Droplet Digital™ PCR (ddPCR™), where parallel nanoliter-scale PCR assays are used to provide a high sensitivity and specificity for DNA detection.

 

However, the recovery of PCR products for downstream applications such as sequencing can be challenging due to the droplets’ stability. Here we compared five methods for disrupting the droplets to recover DNA. We found that rapid freezing in liquid nitrogen results in a clear phase separation and recovery of up to 70% of the DNA content. Liquid nitrogen freezing can thus offer a simple and environmentally friendly protocol for recovering DNA from ddPCR.

 

Classification and review of free PCR primer design software

 

Sensitive detection and quantification of SARS-CoV-2 by multiplex droplet digital RT-PCR

 

The purpose of this study is to develop a one-step droplet digital RT-PCR (RT-ddPCR) multiplex assay that allows for sensitive quantification of SARS-CoV-2 RNA with respect to human-derived RNA and could be used for screening and monitoring of Covid-19 patients. A one-step RT-ddPCR multiplex assay was developed for simultaneous detection of SARS-CoV-2 E, RdRp and N viral RNA, and human Rpp30 DNA and GUSB mRNA, for internal nucleic acid (NA) extraction and RT-PCR control. Dilution series of viral RNA transcripts were prepared in water and total NA extract of Covid-19-negative patients. As reference assay, an E-GUSB duplex RT-PCR was used.

 

GUSB mRNA detection was used to set validity criteria to assure viral RNA and RT-PCR assay quality and to enable quantification of SARS-CoV-2 RNA. In a background of at least 100 GUSB mRNA copies, 5 copies of viral RNA are reliably detectable and 10 copies viral RNA copies are reliably quantifiable. It was found that assay sensitivity of the RT-ddPCR was not affected by the total NA background while assay sensitivity of the gold standard RT-PCR assay is drastically decreased when SARS-CoV-2 copies were detected in a background of total NA extract compared with water. The present study describes a robust and sensitive one-step ddRT-PCR multiplex assay for reliable quantification of SARS-CoV-2 RNA. By determining the fractional abundance of viral RNA with respect to a human housekeeping gene, viral loads from different samples can be compared, what could be used to investigate the infectiveness and to monitor Covid-19 patients.

 

rRT-PCR Results of a Covid-19 Diagnosed Geriatric Patient

 

In this study, we aimed to present a geriatric patient with the diagnosis of COVID-19 and with contradictory results in rRT-PCR examinations in short time intervals. A 69-year-old male patient was admitted to the emergency room on the 18th day of May 2020, with the complaints of fever, sweating, myalgia, dry cough that continued for 5 days, and the lack of taste that started on the day he applied to the emergency room. Comorbidity factors include diabetes mellitus, bronchial asthma, and hypertension. The patient has a history of 36 years of smoking 1.5 packs per day. High laboratory findings during hospitalization: monocytes, creatinine, CRP (C-reactive protein). In the thorax CT, in the parenchyma areas of both lungs, there are increases in attenuation with multilobe distributions (more visible at the level of the upper lobes) in the form of ground-glass opacities.

 

May 19, 2020, was subjected to the rRT-PCR test, repeated twice on the 19th of May which also resulted in positive. Despite rRT-PCR tests, which were negative on 27th of May and positive on 28th of May, the patient, whose symptoms disappeared, and general condition improved, was discharged on June 1, 2020, with the recommendation for home isolation. In our case, unlike the incubation period only, we encountered a negative rRT-PCR result on the 8th day after diagnosis. Therefore, the COVID-19 pandemic control and filiation evaluation with the rRT-PCR test may produce false negative results.

 

HCV Seroconversion Panel Donor# 64273 (10 X 1 mL)
HCV9047	Zeptometrix	10 X 1 mL	EUR 1764

HCV Seroconversion Panel Donor# 64437 (8 X 1 mL)
HCV9048	Zeptometrix	8 X 1 mL	EUR 1035

HCV Seroconversion Panel Donor# 1038851 (5 X 1 mL)
HCV9095	Zeptometrix	5 X 1 mL	EUR 1764

HCV Seroconversion Panel Donor# 61025 (5 X 1 mL)
HCV6217	Zeptometrix	5 X 1 mL	EUR 1035

HCV Seroconversion Panel Donor# 61251 (8 X 1 mL)
HCV6218	Zeptometrix	8 X 1 mL	EUR 1035

HCV Seroconversion Panel Donor# 60749 (14 X 1 mL)
HCV6219	Zeptometrix	14 X 1 mL	EUR 1035

HCV Seroconversion Panel Donor# 63792 (6 X 1 mL)
HCV6220	Zeptometrix	6 X 1 mL	EUR 1035

HCV Seroconversion Panel Donor# 62258 (8 X 1 mL)
HCV6221	Zeptometrix	8 X 1 mL	EUR 1035

HCV Seroconversion Panel Donor# 62286 (8 X 1 mL)
HCV6222	Zeptometrix	8 X 1 mL	EUR 1764

HCV Seroconversion Panel Donor# 62507 (22 X 1 mL)
HCV6223	Zeptometrix	22 X 1 mL	EUR 1035

HCV Seroconversion Panel Donor# 62680 (6 X 1 mL)
HCV6224	Zeptometrix	6 X 1 mL	EUR 1764

HCV Seroconversion Panel Donor# 62999 (19 X 1 mL)
HCV6225	Zeptometrix	19 X 1 mL	EUR 1764

HCV Seroconversion Panel Donor# 63135 (12 X 1 mL)
HCV6226	Zeptometrix	12 X 1 mL	EUR 1764

HCV Seroconversion Panel Donor# 63318 (7 X 1 mL)
HCV6227	Zeptometrix	7 X 1 mL	EUR 1764

HCV Seroconversion Panel Donor# 62886 (12 X 1 mL)
HCV6228	Zeptometrix	12 X 1 mL	EUR 1764

HCV Seroconversion Panel Donor# 62804 (8 X 1 mL)
HCV6229	Zeptometrix	8 X 1 mL	EUR 1764

HCV Seroconversion Panel Donor# 64766 (5 X 1 mL)
HCV9049	Zeptometrix	5 X 1 mL	EUR 1035

HCV Seroconversion Panel Donor# 66158 (13 X 1 mL)
HCV9050	Zeptometrix	13 X 1 mL	EUR 1764

HCV Seroconversion Panel Donor# 66626 (8 X 1 mL)
HCV9054	Zeptometrix	8 X 1 mL	EUR 1764

HCV Seroconversion Panel Donor# 66732 (11 X 1 mL)
HCV9055	Zeptometrix	11 X 1 mL	EUR 1764

HCV Seroconversion Panel Donor# 66861 (11 X 1 mL)
HCV9057	Zeptometrix	11 X 1 mL	EUR 1764

HCV Seroconversion Panel Donor# 66647 (5 X 1 mL)
HCV9058	Zeptometrix	5 X 1 mL	EUR 1764

HCV Seroconversion Panel Donor# 69089 (10 X 1 mL)
HCV10000	Zeptometrix	10 X 1 mL	EUR 1101

HCV Seroconversion Panel Donor# 69654 (7 X 1 mL)
HCV10002	Zeptometrix	7 X 1 mL	EUR 1101

HCV Seroconversion Panel Donor# 69158 (13 X 1 mL)
HCV10003	Zeptometrix	13 X 1 mL	EUR 1101

HCV Seroconversion Panel Donor# 69028 (6 X 1 mL)
HCV10004	Zeptometrix	6 X 1 mL	EUR 1101

HCV Seroconversion Panel Donor# 67355 (11 X 1 mL)
HCV10007	Zeptometrix	11 X 1 mL	EUR 1101

HCV Seroconversion Panel Donor# 69263 (10 X 1 mL)
HCV10008	Zeptometrix	10 X 1 mL	EUR 1101

HCV Seroconversion Panel Donor# 68896 (6 X 1 mL)
HCV10009	Zeptometrix	6 X 1 mL	EUR 1101

HCV Seroconversion Panel Donor# 65470 (7 X 1 mL)
HCV10010	Zeptometrix	7 X 1 mL	EUR 1101

HCV Seroconversion Panel Donor# 66666 (13 X 1 mL)
HCV10011	Zeptometrix	13 X 1 mL	EUR 1101

HCV Seroconversion Panel Donor# 66747 (13 X 1 mL)
HCV10012	Zeptometrix	13 X 1 mL	EUR 1101

HCV Seroconversion Panel Donor# 67356 (10 X 1 mL)
HCV10013	Zeptometrix	10 X 1 mL	EUR 1101

HCV Seroconversion Panel Donor# 68157 (4 X 1 mL)
HCV10014	Zeptometrix	4 X 1 mL	EUR 1101

HCV Seroconversion Panel Donor# 68628 (12 X 1 mL)
HCV10016	Zeptometrix	12 X 1 mL	EUR 1101

HCV Seroconversion Panel Donor# 65064 (16 X 1 mL)
HCV10017	Zeptometrix	16 X 1 mL	EUR 1764

HCV Seroconversion Panel Donor# 69142 (8 X 1 mL)
HCV10019	Zeptometrix	8 X 1 mL	EUR 1101

HCV Seroconversion Panel Donor# 68744 (13 X 1 mL)
HCV10020	Zeptometrix	13 X 1 mL	EUR 1101

HCV Seroconversion Panel Donor# 68470 (14 X 1 mL)
HCV10021	Zeptometrix	14 X 1 mL	EUR 1101

HCV Seroconversion Panel Donor# 68450 (11 X 1 mL)
HCV10022	Zeptometrix	11 X 1 mL	EUR 1101

HCV Seroconversion Panel Donor# 67505 (21 X 1 mL)
HCV10023	Zeptometrix	21 X 1 mL	EUR 1101

HCV Seroconversion Panel Donor# 66719 (8 X 1 mL)
HCV10024	Zeptometrix	8 X 1 mL	EUR 1101

HCV Seroconversion Panel Donor# 66801 (11 X 1 mL)
HCV10025	Zeptometrix	11 X 1 mL	EUR 1101

HCV Seroconversion Panel Donor# 67391 (12 X 1 mL)
HCV10026	Zeptometrix	12 X 1 mL	EUR 1764

HCV Seroconversion Panel Donor# 66751 (8 X 1 mL)
HCV10027	Zeptometrix	8 X 1 mL	EUR 1101

HCV Seroconversion Panel Donor# 67116 (7 X 1 mL)
HCV10028	Zeptometrix	7 X 1 mL	EUR 1101

HCV Seroconversion Panel Donor# 67539 (14 X 1 mL)
HCV10029	Zeptometrix	16 X 1 mL	EUR 1101

HCV Seroconversion Panel Donor# 65977 (8 X 1 mL)
HCV10030	Zeptometrix	8 X 1 mL	EUR 1101

HCV Seroconversion Panel Donor# 66113 (16 X 1 mL)
HCV10032	Zeptometrix	16 X 1 mL	EUR 1101

HCV Seroconversion Panel Donor# 68629 (10 X 1 mL)
HCV10033	Zeptometrix	10 X 1 mL	EUR 1101

HCV Seroconversion Panel Donor# 68223 (13 X 1 mL)
HCV10036	Zeptometrix	13 X 1 mL	EUR 1101

HCV Seroconversion Panel Donor# 65643 (10 X 1 mL)
HCV10043	Zeptometrix	10 X 1 mL	EUR 1101

HCV Seroconversion Panel Donor# 65982 (4 X 1 mL)
HCV10044	Zeptometrix	4 X 1 mL	EUR 1101

HCV Seroconversion Panel Donor# 66074 (5 X 1 mL)
HCV10045	Zeptometrix	5 X 1 mL	EUR 1101

HCV Seroconversion Panel Donor# 67766 (15 X 1 mL)
HCV10050	Zeptometrix	15 X 1 mL	EUR 1101

HCV Seroconversion Panel Donor# 67787 (14 X 1 mL)
HCV10051	Zeptometrix	14 X 1 mL	EUR 1101

HCV Seroconversion Panel Donor# 67861 (12 X 1 mL)
HCV10052	Zeptometrix	12 X 1 mL	EUR 1101

HCV Seroconversion Panel Donor# 420245049 (7 X 1 mL)
HCV10057	Zeptometrix	7 X 1 mL	EUR 1764

HCV Seroconversion Panel Donor# 420299823 (7 X 1 mL)
HCV10058	Zeptometrix	7 X 1 mL	EUR 1858.37
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 490104584 (8 X 1 mL)
HCV10062	Zeptometrix	8 X 1 mL	EUR 1764

HCV Seroconversion Panel Donor# 90163052 (7 X 1 mL)
HCV10071	Zeptometrix	7 X 1 mL	EUR 1764

HCV Seroconversion Panel Donor# 72237 (9 X 1 mL)
HCV10165	Zeptometrix	9 X 1 mL	EUR 1764

HCV Seroconversion Panel Donor# 70225 (5 X 1 mL)
HCV10185	Zeptometrix	5 X 1 mL	EUR 1764

HCV Seroconversion Panel Donor# 22708D (5 X 1 mL)
HCV9094	Zeptometrix	5 X 1 mL	EUR 1764

HCV Pre/Post Seroconversion Panel Donor# 66822 (5 X 1 mL)
HCV10235	Zeptometrix	5 X 1 mL	EUR 545

HIV Seroconversion Panel Donor# 65404 (7 X mL)
HIV9013	Zeptometrix	7 X mL	EUR 1764

EBV Seroconversion Panel Donor# 283843421 (14 X 1 mL)
EBV9097	Zeptometrix	14 X 1 mL	EUR 1764

HIV Seroconversion Panel Donor# 73695 (9 X 1 mL)
HIV12007	Zeptometrix	9 X 1 mL	EUR 2344

HIV Seroconversion Panel Donor# 73698 (13 X 1 mL)
HIV12008	Zeptometrix	13 X 1 mL	EUR 2344

HIV Seroconversion Panel Donor# 63331 (7 X 1 mL)
HIV6248	Zeptometrix	7 X 1 mL	EUR 1764

HIV Seroconversion Panel Donor# 64954 (11 X 1 mL)
HIV9011	Zeptometrix	11 X 1 mL	EUR 1764

HIV Seroconversion Panel Donor# 65389 (8 X 1 mL)
HIV9012	Zeptometrix	8 X 1 mL	EUR 1764

HIV Seroconversion Panel Donor# 65522 (5 X 1 mL)
HIV9014	Zeptometrix	5 X 1 mL	EUR 1764

HIV Seroconversion Panel Donor# 65661 (6 X 1 mL)
HIV9015	Zeptometrix	6 X 1 mL	EUR 1858.37
Description: Please contact Gentaur in order to receive the datasheet of the product.

HIV Seroconversion Panel Donor# 65790 (10 X 1 mL)
HIV9016	Zeptometrix	10 X 1 mL	EUR 1764

HIV Seroconversion Panel Donor# 66575 (10 X 1 mL)
HIV9018	Zeptometrix	10 X 1 mL	EUR 1764

HIV Seroconversion Panel Donor# 65685 (3 X 1 mL)
HIV9019	Zeptometrix	3 X 1 mL	EUR 1764

HIV Seroconversion Panel Donor# 67512 (22 X 1 mL)
HIV9020	Zeptometrix	22 X 1 mL	EUR 1764

HIV Seroconversion Panel Donor# 67485 (17 X 1 mL)
HIV9021	Zeptometrix	17 X 1 mL	EUR 1764

HIV Seroconversion Panel Donor# 64578 (8 X 1 mL)
HIV9022	Zeptometrix	8 X 1 mL	EUR 1764

HIV Seroconversion Panel Donor# 67706 (22 X 1 mL)
HIV9023	Zeptometrix	22 X 1 mL	EUR 1764

HIV Seroconversion Panel Donor# 64469 (12 X 1 mL)
HIV9024	Zeptometrix	12 X 1 mL	EUR 1764

HIV Seroconversion Panel Donor# 67996 (12 X 1 mL)
HIV9025	Zeptometrix	12 X 1 mL	EUR 1764

HIV Seroconversion Panel Donor# 68205 (7 X 1 mL)
HIV9026	Zeptometrix	7 X 1 mL	EUR 1764

HIV Seroconversion Panel Donor# 67860 (7 X 1 mL)
HIV9028	Zeptometrix	7 X 1 mL	EUR 1764

HIV Seroconversion Panel Donor# 68262 (12 X 1 mL)
HIV9029	Zeptometrix	12 X 1 mL	EUR 1764

HIV Seroconversion Panel Donor# 68582 (16 X 1 mL)
HIV9030	Zeptometrix	16 X 1 mL	EUR 1764

HIV Seroconversion Panel Donor# 66048 (19 X 1 mL)
HIV9031	Zeptometrix	19 X 1 mL	EUR 1764

HIV Seroconversion Panel Donor# 66686 (16 X 1 mL)
HIV9033	Zeptometrix	16 X 1 mL	EUR 1764

HIV Seroconversion Panel Donor# 66632 (13 X 1 mL)
HIV9034	Zeptometrix	13 X 1 mL	EUR 1764

HBV Seroconversion Panel Donor# 64090 (5 X 1 mL)
HBV10231	Zeptometrix	5 X 1 mL	EUR 627

HBV Seroconversion Panel Donor# 64017 (6 X 1 mL)
HBV10232	Zeptometrix	6 X 1 mL	EUR 627

HBV Seroconversion Panel Donor# 65449 (9 X 1 mL)
HBV11000	Zeptometrix	9 X 1 mL	EUR 953

HBV Seroconversion Panel Donor# 65524 (8 X 1 mL)
HBV11001	Zeptometrix	8 X 1 mL	EUR 953

HBV Seroconversion Panel Donor# 65556 (6 X 1 mL)
HBV11002	Zeptometrix	6 X 1 mL	EUR 953

HBV Seroconversion Panel Donor# 65584 (8 X 1 mL)
HBV11003	Zeptometrix	8 X 1 mL	EUR 953

HBV Seroconversion Panel Donor# 65732 (8 X 1 mL)
HBV11004	Zeptometrix	8 X 1 mL	EUR 953

HBV Seroconversion Panel Donor# 65777 (14 X 1 mL)
HBV11005	Zeptometrix	14 X 1 mL	EUR 953

HBV Seroconversion Panel Donor# 66201 (17 X 1 mL)
HBV11006	Zeptometrix	17 X 1 mL	EUR 953

HBV Seroconversion Panel Donor# 66537 (14 X 1 mL)
HBV11007	Zeptometrix	14 X 1 mL	EUR 953

HBV Seroconversion Panel Donor# 67303 (18 X 1 mL)
HBV11008	Zeptometrix	18 X 1 mL	EUR 953

HBV Seroconversion Panel Donor# 67449 (23 X 1 mL)
HBV11009	Zeptometrix	23 X 1 mL	EUR 953

HBV Seroconversion Panel Donor# 67457 (18 X 1 mL)
HBV11010	Zeptometrix	18 X 1 mL	EUR 953

HBV Seroconversion Panel Donor# 67694 (14 X 1 mL)
HBV11011	Zeptometrix	14 X 1 mL	EUR 953

HBV Seroconversion Panel Donor# 67773 (6 X 1 mL)
HBV11012	Zeptometrix	6 X 1 mL	EUR 953

HBV Seroconversion Panel Donor# 67962 (35 X 1 mL)
HBV11013	Zeptometrix	35 X 1 mL	EUR 953

HBV Seroconversion Panel Donor# 68029 (12 X 1 mL)
HBV11014	Zeptometrix	12 X 1 mL	EUR 953

HBV Seroconversion Panel Donor# 68105 (14 X 1 mL)
HBV11015	Zeptometrix	14 X 1 mL	EUR 953

HBV Seroconversion Panel Donor# 68541 (10 X 1 mL)
HBV11016	Zeptometrix	10 X 1 mL	EUR 953

HBV Seroconversion Panel Donor# 68739 (14 X 1 mL)
HBV11017	Zeptometrix	14 X 1 mL	EUR 953

HBV Seroconversion Panel Donor# 70793 (14 X 1 mL)
HBV11024	Zeptometrix	14 X 1 mL	EUR 953

HBV Seroconversion Panel Donor# 70970 (16 X 1 mL)
HBV11026	Zeptometrix	16 X 1 mL	EUR 953

HBV Seroconversion Panel Donor# 71415 (13 X 1 mL)
HBV11027	Zeptometrix	13 X 1 mL	EUR 953

HBV Seroconversion Panel Donor# 71840 (10 X 1 mL)
HBV11028	Zeptometrix	10 X 1 mL	EUR 953

HBV Seroconversion Panel Donor# 69751 (13 X 1 mL)
HBV11029	Zeptometrix	13 X 1 mL	EUR 953

HBV Seroconversion Panel Donor# 70292 (15 X 1 mL)
HBV11031	Zeptometrix	15 X 1 mL	EUR 953

HBV Seroconversion Panel Donor# 72439 (13 X 1 mL)
HBV11052	Zeptometrix	13 X 1 mL	EUR 953

HBV Seroconversion Panel Donor# 72474 (11 X 1 mL)
HBV11056	Zeptometrix	11 X 1 mL	EUR 953

HBV Seroconversion Panel Donor# 71612 (8 X 1 mL)
HBV11058	Zeptometrix	8 X 1 mL	EUR 953

HBV Seroconversion Panel Donor# 72324 (9 X 1 mL)
HBV11059	Zeptometrix	9 X 1 mL	EUR 953

HBV Seroconversion Panel Donor# 71922 (11 X 1 mL)
HBV11062	Zeptometrix	11 X 1 mL	EUR 953

HBV Seroconversion Panel Donor# 71782 (9 X 1 mL)
HBV11064	Zeptometrix	9 X 1 mL	EUR 953

HBV Seroconversion Panel Donor# 73299 (15 X 1 mL)
HBV11069	Zeptometrix	15 X 1 mL	EUR 953

HBV Seroconversion Panel Donor# 61248 (5 X 1 mL)
HBV6271	Zeptometrix	5 X 1 mL	EUR 953

HBV Seroconversion Panel Donor# 61291 (25 X 1 mL)
HBV6272	Zeptometrix	25 X 1 mL	EUR 953

HBV Seroconversion Panel Donor# 61042 (6 X 1 mL)
HBV6273	Zeptometrix	6 X 1 mL	EUR 953

HBV Seroconversion Panel Donor# 61799 (7 X 1 mL)
HBV6274	Zeptometrix	7 X 1 mL	EUR 953

HBV Seroconversion Panel Donor# 61066 (7 X 1 mL)
HBV6275	Zeptometrix	7 X 1 mL	EUR 953

HBV Seroconversion Panel Donor# 60409 (8 X 1 mL)
HBV6276	Zeptometrix	8 X 1 mL	EUR 953

HBV Seroconversion Panel Donor# 63291 (11 X 1 mL)
HBV6277	Zeptometrix	11 X 1 mL	EUR 953

HBV Seroconversion Panel Donor# 63426 (10 X 1 mL)
HBV6278	Zeptometrix	10 X 1 mL	EUR 1030.94
Description: Please contact Gentaur in order to receive the datasheet of the product.

HBV Seroconversion Panel Donor# 63701 (7 X 1 mL)
HBV6279	Zeptometrix	7 X 1 mL	EUR 953

HBV Seroconversion Panel Donor# 61832 (5 X 1 mL)
HBV6280	Zeptometrix	5 X 1 mL	EUR 953

HBV Seroconversion Panel Donor# 62433 (12 X 1 mL)
HBV6281	Zeptometrix	12 X 1 mL	EUR 1937

HBV Seroconversion Panel Donor# 62675 (14 X 1 mL)
HBV6282	Zeptometrix	14 X 1 mL	EUR 953

HBV Seroconversion Panel Donor# 62825 (11 X 1 mL)
HBV6283	Zeptometrix	11 X 1 mL	EUR 953

HBV Seroconversion Panel Donor# 62347 (19 X 1 mL)
HBV6284	Zeptometrix	19 X 1 mL	EUR 953

HBV Seroconversion Panel Donor# 62967 (16 X 1 mL)
HBV6285	Zeptometrix	16 X 1 mL	EUR 953

HBV Seroconversion Panel Donor# 63133 (9 X 1 mL)
HBV6286	Zeptometrix	9 X 1 mL	EUR 953

HBV Seroconversion Panel Donor# 63253 (11 X 1 mL)
HBV6287	Zeptometrix	11 X 1 mL	EUR 953

HBV Seroconversion Panel Donor# 63568 (9 X 1 mL)
HBV6288	Zeptometrix	9 X 1 mL	EUR 953

HBV Seroconversion Panel Donor# 63659 (10 X 1 mL)
HBV6289	Zeptometrix	10 X 1 mL	EUR 953

HBV Seroconversion Panel Donor# 63997 (12 X 1 mL)
HBV6290	Zeptometrix	12 X 1 mL	EUR 953

HBV Seroconversion Panel Donor# 64121 (8 X 1 mL)
HBV6291	Zeptometrix	8 X 1 mL	EUR 953

HBV Seroconversion Panel Donor# 64006 (12 X 1 mL)
HBV6292	Zeptometrix	12 X 1 mL	EUR 953

HBV Seroconversion Panel Donor# 64132 (7 X 1 mL)
HBV6293	Zeptometrix	7 X 1 mL	EUR 953

HBV Seroconversion Panel Donor# 66481 (17 X 1 mL)
HBV9072	Zeptometrix	17 X 1 mL	EUR 953

HBV Seroconversion Panel Donor# 65099 (16 X 1 mL)
HBV9073	Zeptometrix	16 X 1 mL	EUR 953

HBV Seroconversion Panel Donor# 66213 (20 X 1 mL)
HBV9074	Zeptometrix	20 X 1 mL	EUR 953

HBV Seroconversion Panel Donor# 1034644 (37 X 1 mL)
HBV9092	Zeptometrix	37 X 1 mL	EUR 2813.09
Description: Please contact Gentaur in order to receive the datasheet of the product.

 

100G Acrylamide
NAT1014	National Diagnostics	100G	EUR 120

500G Acrylamide
NAT1016	National Diagnostics	500G	EUR 261.6

1KG Acrylamide
NAT1018	National Diagnostics	1KG	EUR 412.8

25G AquaPor LE
NAT1020	National Diagnostics	25G	EUR 132

100G AquaPor LE GTAC
NAT1022	National Diagnostics	100G	EUR 250.8

500G AquaPor LE GTAC
NAT1024	National Diagnostics	500G	EUR 878.4

25G AquaPor ES
NAT1026	National Diagnostics	25G	EUR 152.4

100G AquaPor ES
NAT1028	National Diagnostics	100G	EUR 350.4

25G AquaPor LM
NAT1030	National Diagnostics	25G	EUR 288

100G AquaPor LM
NAT1032	National Diagnostics	100G	EUR 860.4

25G AquaPor HR
NAT1034	National Diagnostics	25G	EUR 236.4

100G AquaPor HR
NAT1036	National Diagnostics	100G	EUR 592.8

25G AquaPor 3:1
NAT1038	National Diagnostics	25G	EUR 250.8

100G AquaPor 3:1
NAT1040	National Diagnostics	100G	EUR 570

25G Bis (Methylene Bis-Acrylamide)
NAT1042	National Diagnostics	25G	EUR 100.8

25G DATD Ultrapure
NAT1044	National Diagnostics	25G	EUR 168

250G Glycine
NAT1046	National Diagnostics	250G	EUR 94.8

1KG Glycine Ultrapure
NAT1048	National Diagnostics	1KG	EUR 128.4

250G Tris
NAT1050	National Diagnostics	250G	EUR 102

1KG Tris Ultrapure
NAT1052	National Diagnostics	1KG	EUR 177.6

100G Tricine
NAT1054	National Diagnostics	100G	EUR 120

100G Ion Exchange Resin (mixed bed)
NAT1056	National Diagnostics	100G	EUR 96

25G Riboflavin
NAT1058	National Diagnostics	25G	EUR 96

25ML TEMED
NAT1060	National Diagnostics	25ML	EUR 99.6

25G Ammonium Persulfate
NAT1062	National Diagnostics	25G	EUR 96

100G Ammonium Persulfate
NAT1064	National Diagnostics	100G	EUR 118.8

1G Dithiothreitol (DTT)
NAT1066	National Diagnostics	1G	EUR 103.2

5G Dithiothreitol (DTT)
NAT1068	National Diagnostics	5G	EUR 183.6

50ML 2-Mercaptoethanol
NAT1070	National Diagnostics	50ML	EUR 112.8

100G SDS Ultrapure
NAT1072	National Diagnostics	100G	EUR 111.6

1KG SDS Ultrapure
NAT1074	National Diagnostics	1KG	EUR 457.2

250G Urea
NAT1076	National Diagnostics	250G	EUR 98.4

1KG Urea
NAT1078	National Diagnostics	1KG	EUR 138

450ML Glycerol Ultrapure
NAT1080	National Diagnostics	450ML	EUR 132

200ML Tween 20 Ultrapure
NAT1082	National Diagnostics	200ML	EUR 132

1L Tween 20
NAT1084	National Diagnostics	1L	EUR 169.2

200ML Formamide
NAT1086	National Diagnostics	200ML	EUR 111.6

500ML Boric Acid
NAT1088	National Diagnostics	500ML	EUR 103.2

100G EDTA
NAT1090	National Diagnostics	100G	EUR 94.8

500G EDTA
NAT1092	National Diagnostics	500G	EUR 177.6

GelDry Film 11 X 12 cm
NAT1094	National Diagnostics	PK50	EUR 100.8

25ML Formamide CE
NAT1096	National Diagnostics	25ML	Ask for price

25ML Glass Bond
NAT1098	National Diagnostics	25ML	EUR 158.4

450ML Glass Free
NAT1100	National Diagnostics	450ML	EUR 196.8

GelDry Film 22.5 X 22.5 cm
NAT1102	National Diagnostics	PK50	EUR 130.8

1L DEPC Treated Water
NAT1104	National Diagnostics	1L	EUR 120

Sterling Rapid Silver Stain Kit
NAT1106	National Diagnostics	1KIT	EUR 265.2

1L ProtoBlue Safe
NAT1108	National Diagnostics	1L	EUR 159.6

4L ProtoBlue Safe
NAT1110	National Diagnostics	4L	EUR 282

1L ProtoStain Blue
NAT1112	National Diagnostics	1L	EUR 164.4

25ML Nuclistain
NAT1114	National Diagnostics	25ML	EUR 128.4

450ML AcrylaGel 30%
NAT1116	National Diagnostics	450ML	EUR 128.4

1L AcrylaGel 30%
NAT1118	National Diagnostics	1L	EUR 177.6

450ML Bis-AcrylaGel 2%
NAT1120	National Diagnostics	450ML	EUR 98.4

1L Bis-AcrylaGel 2%
NAT1122	National Diagnostics	1L	EUR 111.6

450ML UreaGel 29:1 Concentrate
NAT1124	National Diagnostics	450ML	EUR 133.2

1L UreaGel 29:1 Concentrate
NAT1126	National Diagnostics	1L	EUR 183.6

UreaGel 29:1 System 1L Kit
NAT1128	National Diagnostics	EACH	EUR 171.6

UreaGel 29:1 System 2.2L Kit
NAT1130	National Diagnostics	EACH	EUR 253.2

450ML SequaGel UreaGel Concentrate
NAT1132	National Diagnostics	450ML	EUR 126

1L SequaGel UreaGel Concentrate
NAT1134	National Diagnostics	1L	EUR 160.8

SequaGel Sequencing System 1L Kit
NAT1136	National Diagnostics	1KIT	EUR 171.6

SequaGel Sequencing System 2.2L Kit
NAT1138	National Diagnostics	EACH	EUR 253.2

100ML SequaGel Buffer
NAT1140	National Diagnostics	100ML	EUR 80.4

200ML SequaGel UreaGel Buffer
NAT1142	National Diagnostics	200ML	EUR 84

450ML SequaGel UreaGel-6
NAT1144	National Diagnostics	450ML	EUR 115.2

1L SequaGel UreaGel-6
NAT1146	National Diagnostics	1L	EUR 151.2

450ML SequaGel UreaGel-8
NAT1148	National Diagnostics	450ML	EUR 115.2

1L SequaGel UreaGel-8
NAT1150	National Diagnostics	1L	EUR 151.2

450ML SequaGel UreaGel Diluent
NAT1152	National Diagnostics	450ML	EUR 90

1L SequaGel UreaGel Diluent
NAT1154	National Diagnostics	1L	EUR 103.2

90ML SequaGel Complete Buffer
NAT1156	National Diagnostics	90ML	EUR 79.2

200ML SequaGel UreaGel Complete Buffer
NAT1158	National Diagnostics	200ML	EUR 85.2

450ML SequaGel XR
NAT1160	National Diagnostics	450ML	EUR 126

1L SequaGel XR
NAT1162	National Diagnostics	1L	EUR 165.6

100ML SequaGel XR Concentrate
NAT1164	National Diagnostics	100ML	EUR 128.4

450ML SequaGel XR Concentrate
NAT1166	National Diagnostics	450ML	EUR 361.2

200ML SequaGel MD Monomer Solution
NAT1168	National Diagnostics	200ML	EUR 220.8

SequaGel MD SSCP Kit
NAT1170	National Diagnostics	1KIT	EUR 253.2

SequaGel MD Heteroduplex Kit
NAT1172	National Diagnostics	EACH	EUR 265.2