Evaluation of PCR Positive Cases in the Pediatric Emergency Department

November 12, 2020
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mhealthinfo

A rapid, high-volume cervical screening project using self-sampling and isothermal PCR HPV testing

 

Objective: Rapid, high-volume screening programs are needed as part of cervical cancer prevention in China.
Methods: In a 5-day screening project in Inner Mongolia, 3345 women volunteered following a community awareness campaign, and self-swabbed to permit rapid HPV testing. Two AmpFire™ HPV detection systems (Atila Biosystems) were sufficient to provide pooled 15-HPV type data within an hour. HPV+ patients had same-day digital colposcopy (DC) performed by 1 of 6 physicians, using the EVA™ system (MobileODT). Digital images were obtained and, after biopsy of suspected lesions for later confirmatory diagnosis, women were treated immediately based on colposcopic impression. Suspected low- grade lesions were offered treatment with thermal ablation (Wisap), and suspected high-grade lesions were treated with LLETZ.
Results: Of 3345 women screened, 624 (18.7%) were HPV+. Of these, 88.5% HPV+ women underwent same-day colposcopy and 78 were treated. Later consensus histology results obtained on 197 women indicated 20 CIN2+, of whom 15 were detected and treated/referred at screening (10 by thermal ablation, 4 by LLETZ, 1 by referral).
Conclusions: Global control of cervical cancer will require both vaccination and screening of a huge number of women. This study illustrates a cervical screening strategy that can be used to screen-and-treat large numbers of women. HPV self-sampling facilitates high-volume screening. Specimens can be tested rapidly, promoting minimal loss-to-follow-up.
Specifically, the AmpFire™ system used in this study is highly portable, simple, rapid (92 specimens per 65 min per unit), and economical. Visual triage can be performed on HPV+ women with a portable digital colposcope that provides magnification, lighting, and a recorded image. Diagnosis and appropriate treatment remain the most subjective elements. The digital image is under study for deep-learning based automated evaluation that could assist the management decision, either by itself or combined with HPV typing.

[The Identification of Acinetobacter baumannii Blood Isolates by MALDI-TOF MS, ARDRA and blaOXA-51-like Gene-Specific Real-Time PCR]

The Acinetobacter calcoaceticus-Acinetobacter baumannii (Acb) complex consists of phenotypically very similar nosocomial species; A.baumannii, Acinetobacter nosocomialis, Acinetobacter pittii, Acinetobacter seifertii and Acinetobacter djikshoorniae and one environmental species A.calcoaceticus. The rapid and accurate identification of the members of Acb complex is critical as these nosocomial pathogens can show differences in antimicrobial susceptibility and clinical outcomes.
The conventional phenotypic methods are slow, unreliable and less efficient for the differentiation of Acb complex species, including the A.baumannii species within the Acb complex. Although various molecular methods are available, such as amplified ribosomal DNA restriction analysis (ARDRA) and blaOXA-51-like gene specific PCR, they are usually inconvenient for the routine diagnostic laboratories. Recently, matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) offers an opportunity for rapid, cost-effective and convenient bacterial identification in routine diagnostic procedures conducted in clinical laboratory. In this study, we aimed to evaluate the diagnosis performance of MALDI-TOF MS system to identify blood isolates of A.baumannii. A total of 180 nonduplicate carbapenem resistant Acb complex (strain numbers; TR1-TR60) and A.baumannii (TR61-TR180) blood isolates were collected from the intensive care units of the three university hospitals in Turkey from January 2016 to December 2016.
All isolates were evaluated by using blaOXA-51-like gene specific real time (Rt-PCR) analysis, ARDRA (restriction enzymes-AluI, CfoI, MboI, MspI, RsaI) method and MALDI-TOF MS (VITEK® MS, bioMérieux, France) system. All the strains except TR10, TR31, TR35 and TR52 were identified as A.baumannii by ARDRA method. Out of 177 of all the isolates, presence of blaOXA-51-like gene was found except for TR10, TR31 and TR52 isolates. However, TR31 without the presence of blaOXA-51-like gene was identified as A.pittii using the ARDRA. Totally 176 isolates which were identified as A.baumannii by both of the methods, ARDRA and Rt-PCR- blaOXA-51-like, were accepted as a reference for the evaluation of the diagnosis performance capacity of the MALDI-TOF MS. Overall, for all 176 isolates tested, the sensitivity obtained with the MALDI-TOF MS were 99.4% with 75% specificity.
The accuracy value of the method was determined as 98.9% for the identification of A.baumannii to the species level. MALDI-TOF MS is increasingly used in diagnostic microbiology for the routine identification of bacteria to the genus, species or subspecies level with high rates of sensitivity and specificity. In future, by expanding the database, MALDI-TOF MS system would possibly become the ideal method for routine diagnostic laboratories that could potentially identify more species and even determine some characteristics of antimicrobial resistance and virulence determinants.

Evaluation of SARS-CoV-2 IgG antibody response in PCR positive patients: Comparison of nine tests in relation to clinical data

 

SARS-CoV-2 antibody tests are available in various formats, detecting different viral target proteins and antibody subclasses. The specificity and sensitivity of SARS-CoV-2 antibody tests are known to vary and very few studies have addressed the performance of these tests in COVID-19 patient groups at different time points. We here compared the sensitivity and specificity of seven commercial (SNIBE, Epitope, Euroimmun, Roche, Abbott, DiaSorin, Biosensor) and two in-house LIPS assays (LIPS N and LIPS S-RBD) IgG/total Ab tests in serum samples from 97 COVID-19 patients and 100 controls, and correlated the results with the patients’ clinical data and the time-point the test was performed. We found a remarkable variation in the sensitivity of antibody tests with the following performance: LIPS N (91.8%), Epitope (85.6%), Abbott and in-house LIPS S-RBD (both 84.5%), Roche (83.5%), Euroimmun (82.5%), DiaSorin (81.4%), SNIBE (70.1%), and Biosensor (64.9%).
The overall agreement between the tests was between 71-95%, whereas the specificity of all tests was within 98-100%. The correlation with patients’ clinical symptoms score ranged from strongest in LIPS N (ρ = 0.41; p<0.001) to nonsignificant in LIPS S-RBD. Furthermore, the time of testing since symptom onset had an impact on the sensitivity of some tests. Our study highlights the importance to consider clinical symptoms, time of testing, and using more than one viral antigen in SARS-CoV-2 antibody testing. Our results suggest that some antibody tests are more sensitive for the detection of antibodies in early stage and asymptomatic patients, which may explain the contradictory results of previous studies and should be taken into consideration in clinical practice and epidemiological studies.

Monkeypox Virus Real Time PCR Kit
PDPS-AR064 1 unit Ask for price
Description: Creative Biogene Monkeypox Virus Real Time PCR Kit is used for the detection of monkeypox Virus in serum or lesion exudate samples by using real time PCR systems. Monkeypox virus (MPV) is a double-stranded DNA, zoonotic virus and a species of the genus Orthopoxvirus in the family Poxviridae. It is one of the human orthopoxviruses that includes variola (VARV), cowpox (CPX), and vaccinia (VACV) viruses. The kit contains a specific ready-to-use system for the detection of the monkeypox Virus. Fluorescence is emitted and measured by the real time systems' optical unit during the PCR.

Monkeypox Virus Real Time PCR Kit
YJC70115NW-25T 25 tests/kit Ask for price
Description: The Bioperfectus Monkeypox Virus Real Time PCR Kit is an in vitro diagnostic test, based on real-time PCR technology, for the detection of DNA from the Monkeypox virus. Specimens can be obtained from human serum, lesion exudate samples and scab. BSL-2 facilities with standard BSL-2 work practices may be used for the test of t he Monkeypox virus.

Monkeypox Virus Real Time PCR Kit
ZD-0076-01 25 tests/kit Ask for price
Description: Monkeypox virus is the virus that causes the disease monkeypox in both humans and animals. Monkeypox virus is an Orthopoxvirus, a genus of the family Poxviridae that contains other viral species that target mammals. The virus is mainly found in tropical rainforest regions of central and West Africa. The primary route of infection is thought to be contact with the infected animals or their bodily fluids. The genome is not segmented and contains a single molecule of linear double-stranded DNA, 185000 nucleotides long. The Monkeypox Virus real time PCR Kit contains a specific ready-to-use system for the detection of the Monkeypox Virusthrough polymerase chain reaction (PCR) in the real-time PCR system. The master contains reagents and enzymes for the specific amplification of the Monkeypox Virus DNA. Fluorescence is emitted and measured by the real time systems ́ optical unit during the PCR. The detection of amplified Monkeypox Virus DNA fragment is performed in fluorimeter channel 530nm with the fluorescent quencher BHQ1. DNA extraction buffer is available in the kit and serum or lesion exudate samples are used for the extraction of the DNA. In addition, the kit contains a system to identify possible PCR inhibition by measuring the 560nm fluorescence of the internal control (IC). An external positive control defined as 1×10^7 copies/ml is supplied which allow the determination of the gene load.

Monkeypox Virus Real Time PCR Kit
ZD-0076-02 25 tests/kit Ask for price
Description: Monkeypox virus is the virus that causes the disease monkeypox in both humans and animals. Monkeypox virus is an Orthopoxvirus, a genus of the family Poxviridae that contains other viral species that target mammals. The virus is mainly found in tropical rainforest regions of central and West Africa. The primary route of infection is thought to be contact with the infected animals or their bodily fluids.The genome is not segmented and contains a single molecule of linear double-stranded DNA, 185000 nucleotides long.The Monkeypox Virus real time PCR Kit contains a specific ready-to-use system for the detection of the Monkeypox Virusthrough polymerase chain reaction (PCR) in the real-time PCR system. The master contains reagents and enzymes for the specific amplification of theMonkeypox VirusDNA. Fluorescence is emitted and measured by the real time systems ́ optical unit during the PCR. The detection of amplified Monkeypox Virus DNA fragment is performed in fluorimeter channelFAM with the fluorescent quencher BHQ1. DNA extraction buffer is available in the kit and serum or lesion exudate samples are used for the extraction of the DNA. In addition, the kit contains a system to identify possible PCR inhibition by measuring the HEX/VIC/JOE fluorescence of the internal control (IC). An external positive control defined as 1×107copies/ml is supplied which allow the determination of the gene load.

lyophilised real-time PCR Master Mix for dual labeled probes
M-PCR-156L 960 reactions x 20 µl
EUR 900.68
Description: lyophilised real-time PCR Master Mix for dual labeled probes

lyophilised real-time PCR Master Mix for dual labeled probes
M-PCR-156S 192 reactions x 20 µl
EUR 225.3
Description: lyophilised real-time PCR Master Mix for dual labeled probes

Novel Coronavirus COVID-19 (2019-nCoV) Real Time RT-PCR Kit
RR-0478-02 25 tests/kit
EUR 1189.2
Description: Novel Coronavirus (2019-nCoV) Real Time RT-PCR Kit is used for the qualitative detection of a novel coronavirus, which was identified in 2019 at Wuhan City, Hubei Province, China, in upper respiratory tract specimens (nasopharyngeal extracts, deep cough sputum, etc.) and lower respiratory tract specimens (alveoli irrigation fluid, etc.) by real time PCR systems.

dsGreen for Real-Time PCR, 100×, 2 mL
61010 2 mL
EUR 435.6

0,1ML THIN WALL TUBES 8 PER STRIP. CLEAR & REAL TIME STRIP CAPS
PCR-0108-LP-RT-C 125/pk
EUR 850.8
Description: PCR Plates & Tubes; PCR Strip Tubes and Strip Caps - Axygen

0,1ML THIN WALL TUBES 8 PER STRIP. WHITE & REAL TIME STRIP CAPS
PCR-0108-LP-RT-W 125/pk
EUR 924
Description: PCR Plates & Tubes; PCR Strip Tubes and Strip Caps - Axygen

hsa-mir-521 Real-Time RT-PCR Detection and U6 Calibration Kit
20-abx097601
  • EUR 878.40
  • EUR 1245.60
  • EUR 627.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

TurboQ Real-time PCR Machine with Laptop
MOL2700 EACH
EUR 30805.08

lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain
M-PCR-173L 960 reactions x 20 µl
EUR 789.36
Description: lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain

lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain
M-PCR-173S 192 reactions x 20 µl
EUR 197.34
Description: lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain

dsGreen for Real-Time PCR, 100×, 5 mL
71010 5 mL
EUR 750

dsGreen for Real-Time PCR, 100×, 1 mL
41010 1 mL
EUR 298.8

dsGreen for Real-Time PCR, 100×, 10 mL
81010 10 mL
EUR 1042.8

mmu-mir-24-2-5p Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097869
  • EUR 878.40
  • EUR 1245.60
  • EUR 627.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

PMA Real-Time PCR Bacterial Viability Kit - Staphylococcus aureus (mecA)
31036 1kit
EUR 478.8
Description: Minimum order quantity: 1 unit of 1kit

PMA Real-Time PCR Bacterial Viability Kit - E. coli (uidA)
31050 1kit
EUR 513.6
Description: Minimum order quantity: 1 unit of 1kit

PMA Real-Time PCR Bacterial Viability Kit - Listeria monocytogenes (hly)
31051 1kit
EUR 478.8
Description: Minimum order quantity: 1 unit of 1kit

PMA Real-Time PCR Bacterial Viability Kit - Legionella pneumophila (mip)
31053 1kit
EUR 513.6
Description: Minimum order quantity: 1 unit of 1kit

hsa-let-7a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097603
  • EUR 878.40
  • EUR 1245.60
  • EUR 627.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-let-7b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097604
  • EUR 878.40
  • EUR 1245.60
  • EUR 627.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-let-7c Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097605
  • EUR 878.40
  • EUR 1245.60
  • EUR 627.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-let-7d Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097606
  • EUR 878.40
  • EUR 1245.60
  • EUR 627.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-let-7e Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097607
  • EUR 878.40
  • EUR 1245.60
  • EUR 627.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-let-7f Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097608
  • EUR 878.40
  • EUR 1245.60
  • EUR 627.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-let-7g Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097609
  • EUR 878.40
  • EUR 1245.60
  • EUR 627.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-let-7i Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097610
  • EUR 878.40
  • EUR 1245.60
  • EUR 627.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-let-7f Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097611
  • EUR 878.40
  • EUR 1245.60
  • EUR 627.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-1 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097612
  • EUR 878.40
  • EUR 1245.60
  • EUR 627.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-7 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097613
  • EUR 878.40
  • EUR 1245.60
  • EUR 627.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-9 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097614
  • EUR 878.40
  • EUR 1245.60
  • EUR 627.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-9* Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097615
  • EUR 878.40
  • EUR 1245.60
  • EUR 627.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-10a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097616
  • EUR 878.40
  • EUR 1245.60
  • EUR 627.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-10b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097617
  • EUR 878.40
  • EUR 1245.60
  • EUR 627.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-10a* Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097618
  • EUR 878.40
  • EUR 1245.60
  • EUR 627.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-15a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097619
  • EUR 878.40
  • EUR 1245.60
  • EUR 627.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-15b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097620
  • EUR 878.40
  • EUR 1245.60
  • EUR 627.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-16 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097622
  • EUR 878.40
  • EUR 1245.60
  • EUR 627.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-18a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097625
  • EUR 878.40
  • EUR 1245.60
  • EUR 627.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-18b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097626
  • EUR 878.40
  • EUR 1245.60
  • EUR 627.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-19a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097627
  • EUR 878.40
  • EUR 1245.60
  • EUR 627.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-19b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097628
  • EUR 878.40
  • EUR 1245.60
  • EUR 627.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-20a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097629
  • EUR 878.40
  • EUR 1245.60
  • EUR 627.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-20b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097630
  • EUR 878.40
  • EUR 1245.60
  • EUR 627.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-21 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097631
  • EUR 878.40
  • EUR 1245.60
  • EUR 627.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-22 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097632
  • EUR 878.40
  • EUR 1245.60
  • EUR 627.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-23a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097633
  • EUR 878.40
  • EUR 1245.60
  • EUR 627.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-23b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097634
  • EUR 878.40
  • EUR 1245.60
  • EUR 627.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-25 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097636
  • EUR 878.40
  • EUR 1245.60
  • EUR 627.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-26a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097637
  • EUR 878.40
  • EUR 1245.60
  • EUR 627.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-26b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097638
  • EUR 878.40
  • EUR 1245.60
  • EUR 627.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-27a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097639
  • EUR 878.40
  • EUR 1245.60
  • EUR 627.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-27b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097640
  • EUR 878.40
  • EUR 1245.60
  • EUR 627.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-29a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097642
  • EUR 878.40
  • EUR 1245.60
  • EUR 627.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-29b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097643
  • EUR 878.40
  • EUR 1245.60
  • EUR 627.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-29c Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097644
  • EUR 878.40
  • EUR 1245.60
  • EUR 627.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-30a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097645
  • EUR 878.40
  • EUR 1245.60
  • EUR 627.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-30b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097647
  • EUR 878.40
  • EUR 1245.60
  • EUR 627.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-30c Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097648
  • EUR 878.40
  • EUR 1245.60
  • EUR 627.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-30d Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097650
  • EUR 878.40
  • EUR 1245.60
  • EUR 627.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-30e Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097651
  • EUR 878.40
  • EUR 1245.60
  • EUR 627.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-31 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097652
  • EUR 878.40
  • EUR 1245.60
  • EUR 627.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-32 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097653
  • EUR 878.40
  • EUR 1245.60
  • EUR 627.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-33a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097654
  • EUR 878.40
  • EUR 1245.60
  • EUR 627.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-34a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097657
  • EUR 878.40
  • EUR 1245.60
  • EUR 627.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-34b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097659
  • EUR 878.40
  • EUR 1245.60
  • EUR 627.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-92a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097661
  • EUR 878.40
  • EUR 1245.60
  • EUR 627.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-92b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097662
  • EUR 878.40
  • EUR 1245.60
  • EUR 627.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-93 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097663
  • EUR 878.40
  • EUR 1245.60
  • EUR 627.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-95 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097664
  • EUR 878.40
  • EUR 1245.60
  • EUR 627.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-96 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097665
  • EUR 878.40
  • EUR 1245.60
  • EUR 627.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-98 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097666
  • EUR 878.40
  • EUR 1245.60
  • EUR 627.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-99a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097667
  • EUR 878.40
  • EUR 1245.60
  • EUR 627.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-99b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097669
  • EUR 878.40
  • EUR 1245.60
  • EUR 627.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-100 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097671
  • EUR 878.40
  • EUR 1245.60
  • EUR 627.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-101 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097673
  • EUR 878.40
  • EUR 1245.60
  • EUR 627.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-103 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097675
  • EUR 878.40
  • EUR 1245.60
  • EUR 627.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-105 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097676
  • EUR 878.40
  • EUR 1245.60
  • EUR 627.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-106a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097677
  • EUR 878.40
  • EUR 1245.60
  • EUR 627.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-106b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097678
  • EUR 878.40
  • EUR 1245.60
  • EUR 627.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-107 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097679
  • EUR 878.40
  • EUR 1245.60
  • EUR 627.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-124 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097680
  • EUR 878.40
  • EUR 1245.60
  • EUR 627.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-125a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097681
  • EUR 878.40
  • EUR 1245.60
  • EUR 627.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-127 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097686
  • EUR 878.40
  • EUR 1245.60
  • EUR 627.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-129 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097689
  • EUR 878.40
  • EUR 1245.60
  • EUR 627.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-130a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097690
  • EUR 878.40
  • EUR 1245.60
  • EUR 627.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-130b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097691
  • EUR 878.40
  • EUR 1245.60
  • EUR 627.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-132 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097692
  • EUR 878.40
  • EUR 1245.60
  • EUR 627.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-133a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097693
  • EUR 878.40
  • EUR 1245.60
  • EUR 627.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-133b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097694
  • EUR 878.40
  • EUR 1245.60
  • EUR 627.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-134 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097695
  • EUR 878.40
  • EUR 1245.60
  • EUR 627.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-135b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097696
  • EUR 878.40
  • EUR 1245.60
  • EUR 627.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-137 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097699
  • EUR 878.40
  • EUR 1245.60
  • EUR 627.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-138 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097700
  • EUR 878.40
  • EUR 1245.60
  • EUR 627.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-139 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097701
  • EUR 878.40
  • EUR 1245.60
  • EUR 627.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-140 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097702
  • EUR 878.40
  • EUR 1245.60
  • EUR 627.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-141 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097703
  • EUR 878.40
  • EUR 1245.60
  • EUR 627.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

[Evaluation of SARS-CoV-2 PCR Positive Cases in the Pediatric Emergency Department]

 

In December 2019, a previously unknown type of coronavirus was detected in China and named as “severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2)”. The World Health Organization has named the SARS-CoV-2 related as coronavirus disease-2019 (COVID-19) and declared it as a pandemic. There is a limited data about the COVID-19 disease for the pediatric patients. In this study, it was aimed to evaluate the epidemiological, clinical, laboratory and radiologic findings, treatment and clinical outcomes of patients admitted to the pediatric emergency department with the suspicion of COVID-19. Between March 11 and June 16, 2020, patients aged between 1 month-18 years admitted to the pediatric emergency department and who have an indication for sampling for the polymerase chain reaction (PCR) method with the suspicion of COVID-19 according to the current guidelines published by the Ministry of Health were included in the study. The demographic characteristics, symptoms, durations and the history of contact with the suspected/definite COVID-19 cases were questioned in the patients with positive results. Physical examination, laboratory and imaging data of the patients were recorded. According to clinical severity, patients were divided into five groups.
mhealthinfo

mhealthinfo

Treatment methods, ward/intensive care unit admission, length of stay at hospital, and prognosis were recorded. Of the 237 patients included in the study, 45 (18.9%) of the samples were positive and 192 (81.1%) were negative. There was a history of contact with COVID-19 positive case in 38 (85.6%) of COVID-19 PCR positive patients. The mean time for onset of symptoms after contact was 3.5 ± 1.7 days. Twenty-one of the patients (46.6%) were asymptomatic and the most common symptom was fever (34.1%) and cough (27.3%). Of the patients whose laboratory tests were requested, lymphopenia wasdetected in 50% and 52.3% of procalcitonin, 23.5% of C-reactive protein and 64.7% of D-dimer values were found to be high.
Chest radiography was obtained from 45.4% of the patients; 90.0% were evaluated as normal, bronchovascular change, pleural effusion and consolidation were detected in one of each (5.0%) patient. Thorax computed tomography (CT) was obtained from 4 (9.0%) patients. One patient had normal CT findings, two patients had consolidation, one patient had peripheral ground-glass appearance and one patient had pleural effusion. Antibiotics were started in 38.6% of the patients and the most commonly used antibiotic was azithromycin (34.1%). Oseltamivir was started in one (2.3%) patient, and 10 (24.7%) patients were treated with hydroxychloroquine.
There were no serious and critical cases according to the clinical severity. Pediatric patients constitute a small part of COVID-19 individuals in the community, and a significant part of them are asymptomatic, and patients who are symptomatic present with a mild clinic. In our study, most of the patients had a history of contact with COVID-19 positive cases, therefore, it should be questioned when evaluating a pediatric patient. There were no specific findings for COVID-19 positive patients in terms of laboratory and radiology.

Alpha-bungarotoxin, CF405s
00002-100ug 100uG
EUR 193.2
Description: Minimum order quantity: 1 unit of 100uG

Alpha-bungarotoxin, CF680r
9-00003
  • EUR 592.80
  • EUR 193.20
  • 500uG
  • 100uG
Description: Minimum order quantity: 1 unit of 500uG

Alpha-bungarotoxin, CF680r
9-00003
  • EUR 592.80
  • EUR 193.20
  • 500uG
  • 100uG
Description: Minimum order quantity: 1 unit of 100uG

Alpha-bungarotoxin, CF640r
9-00004
  • EUR 592.80
  • EUR 193.20
  • 500uG
  • 100uG
Description: Minimum order quantity: 1 unit of 500uG

Alpha-bungarotoxin, CF640r
9-00004
  • EUR 592.80
  • EUR 193.20
  • 500uG
  • 100uG
Description: Minimum order quantity: 1 unit of 100uG

Alpha-bungarotoxin, CF488a
9-00005
  • EUR 592.80
  • EUR 193.20
  • 500uG
  • 100uG
Description: Minimum order quantity: 1 unit of 500uG

Alpha-bungarotoxin CF488a
9-00005
  • EUR 592.80
  • EUR 193.20
  • 500uG
  • 100uG
Description: Minimum order quantity: 1 unit of 100uG

Alpha-bungarotoxin, CF568
9-00006
  • EUR 592.80
  • EUR 193.20
  • 500uG
  • 100uG
Description: Minimum order quantity: 1 unit of 500uG

Alpha-bungarotoxin, CF568
9-00006
  • EUR 592.80
  • EUR 193.20
  • 500uG
  • 100uG
Description: Minimum order quantity: 1 unit of 100uG

Alpha-bungarotoxin, CF594
9-00007
  • EUR 592.80
  • EUR 193.20
  • 500uG
  • 100uG
Description: Minimum order quantity: 1 unit of 500uG

Alpha-bungarotoxin CF594
9-00007
  • EUR 592.80
  • EUR 193.20
  • 500uG
  • 100uG
Description: Minimum order quantity: 1 unit of 100uG

Alpha-bungarotoxin, CF633
9-00009
  • EUR 592.80
  • EUR 193.20
  • 500uG
  • 100uG
Description: Minimum order quantity: 1 unit of 500uG

Alpha-bungarotoxin, CF633
9-00009
  • EUR 592.80
  • EUR 193.20
  • 500uG
  • 100uG
Description: Minimum order quantity: 1 unit of 100uG

Alpha-bungarotoxin
00010-1 1MG
EUR 201.6
Description: Minimum order quantity: 1 unit of 1MG

Fluorescein-Alpha-bungarotoxin
00011 500uG
EUR 440.4
Description: Minimum order quantity: 1 unit of 500uG

Tetramethylrhodamine-Alpha-bungarotoxin
00012 500uG
EUR 459.6
Description: Minimum order quantity: 1 unit of 500uG

Fluorescein-alpha-bungarotoxin: (10x50ug)
00013 10ST
EUR 500.4
Description: Minimum order quantity: 1 unit of 10ST

Tetramethylrhodamine-alpha-bungarotoxin: (10x50ug)
00014 10ST
EUR 559.2
Description: Minimum order quantity: 1 unit of 10ST

Sulforhodamine 101-a-bungarotoxin (Texas Red--a-bungarotoxin): (500ug)
00015 500uG
EUR 559.2
Description: Minimum order quantity: 1 unit of 500uG

Sulforhodamine 101-a-bungarotoxin (Texas Red--a-bungarotoxin): (10x50ug)
00016 10ST
EUR 627.6
Description: Minimum order quantity: 1 unit of 10ST

Biotin-xx-a-bungarotoxin
00017 500uG
EUR 519.6
Description: Minimum order quantity: 1 unit of 500uG

Alpha-Bungarotoxin, CF555 conjugate
9-00018
  • EUR 592.80
  • EUR 193.20
  • 500uG
  • 100ug
Description: Minimum order quantity: 1 unit of 500uG

Alpha-Bungarotoxin, CF555 conjugate
9-00018
  • EUR 592.80
  • EUR 193.20
  • 500uG
  • 100ug
Description: Minimum order quantity: 1 unit of 100ug

Biotin-cAMP, diisopropylethylammonium salt:
00020 1MG
EUR 376.8
Description: Minimum order quantity: 1 unit of 1MG

Biotin-cAMP, diisopropylethylammonium salt: (20x50ug)
00020-1 20ST
EUR 501.6
Description: Minimum order quantity: 1 unit of 20ST

Biotin-cGMP, diisopropylethylammonium salt:
00021 1MG
EUR 412.8
Description: Minimum order quantity: 1 unit of 1MG

Biotin-cGMP, diisopropylethylammonium salt: (20x50ug)
00021-1 20ST
EUR 537.6
Description: Minimum order quantity: 1 unit of 20ST

Cyanine 644-cAMP:
00022 1MG
EUR 589.2
Description: Minimum order quantity: 1 unit of 1MG

Cyanine 644-cAMP: (20x50ug)
00022-1 20ST
EUR 750
Description: Minimum order quantity: 1 unit of 20ST

Fluorescein Methotrexate, triammonium salt:
00023 1MG
EUR 337.2
Description: Minimum order quantity: 1 unit of 1MG

Staurosporine
00025 100uG
EUR 166.8
Description: Minimum order quantity: 1 unit of 100uG

Alpha-bungarotoxin, CF543
00026 500uG
EUR 592.8
Description: Minimum order quantity: 1 unit of 500uG

Rhodamine phalloidin 300u
00027 300
EUR 414
Description: Minimum order quantity: 1 unit of 300

Biotin-xx-phalloidin
00028 100U
EUR 513.6
Description: Minimum order quantity: 1 unit of 100U

Fluorescein-phalloidin
00030 300U
EUR 414
Description: Minimum order quantity: 1 unit of 300U

Rhodamine 110 phalloidin
00032 300ST
EUR 414
Description: Minimum order quantity: 1 unit of 300ST

Sulforhodamine 101 (Texas-Red) Phalloidin
00033 300EU
EUR 414
Description: Minimum order quantity: 1 unit of 300EU

Phalloidin, CF405M
00034 300U
EUR 530.4
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF405M
00034-T 50U
EUR 159.6
Description: Minimum order quantity: 1 unit of 50U

CF488A-cAMP
00036 100ug
EUR 206.4
Description: Minimum order quantity: 1 unit of 100ug

CF640R-cAMP
00037 100ug
EUR 206.4
Description: Minimum order quantity: 1 unit of 100ug

Phalloidin, CF555
00040 300U
EUR 530.4
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF555
00040-T 50U
EUR 159.6
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF647
00041 300U
EUR 530.4
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF647
00041-T 50U
EUR 159.6
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF488A
00042 300U
EUR 530.4
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF488A
00042-T 50U
EUR 159.6
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF543
00043 300U
EUR 530.4
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF543
00043-T 50U
EUR 159.6
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF568
00044 300U
EUR 530.4
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF568
00044-T 50U
EUR 159.6
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF594
00045 300U
EUR 530.4
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF594
00045-T 50U
EUR 159.6
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF633
00046 300U
EUR 530.4
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF633
00046-T 50U
EUR 159.6
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF660R
00047 300U
EUR 530.4
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF660R
00047-T 50U
EUR 159.6
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF680R
00048 300U
EUR 559.2
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF680R
00048-T 50U
EUR 159.6
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF350
00049 300U
EUR 530.4
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF350
00049-T 50U
EUR 159.6
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF640R
00050 300U
EUR 530.4
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF640R
00050-T 50U
EUR 159.6
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF532
00051 300U
EUR 530.4
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF532
00051-T 50U
EUR 159.6
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF660C
00052 300U
EUR 530.4
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF660C
00052-T 50U
EUR 159.6
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF680
00053 300U
EUR 559.2
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF680
00053-T 50U
EUR 176.4
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF430
00054 300U
EUR 538.8
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF430
00054-T 50U
EUR 166.8
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF440
00055 300U
EUR 538.8
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF440
00055-T 50U
EUR 166.8
Description: Minimum order quantity: 1 unit of 50U

Cholera Toxin Subunit B, CF488A conjugate
00070 100ug
EUR 313.2
Description: Minimum order quantity: 1 unit of 100ug

Cholera Toxin Subunit B, CF568 conjugate
00071 100ug
EUR 313.2
Description: Minimum order quantity: 1 unit of 100ug

Cholera Toxin Subunit B, CF594 conjugate
00072 100ug
EUR 313.2
Description: Minimum order quantity: 1 unit of 100ug

Cholera Toxin Subunit B, CF640R conjugate
00073 100ug
EUR 316.8
Description: Minimum order quantity: 1 unit of 100ug

Cholera Toxin Subunit B, CF532 conjugate
00074 100ug
EUR 316.8
Description: Minimum order quantity: 1 unit of 100ug

Cholera Toxin Subunit B, CF543 conjugate
00075 100ug
EUR 316.8
Description: Minimum order quantity: 1 unit of 100ug

Cholera Toxin Subunit B, CF620R conjugate
00076 100ug
EUR 316.8
Description: Minimum order quantity: 1 unit of 100ug

Cholera Toxin Subunit B, CF633 conjugate
00077 100ug
EUR 316.8
Description: Minimum order quantity: 1 unit of 100ug
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