Molecular Detection of the mcr Genes by Multiplex PCR

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Complete antigen-bridged DNA strand displacement amplification immuno-PCR assay for ultrasensitive detection of salbutamol

 

Monitoring of low-level analytes are typical examples for analytical challenges. Salbutamol (SAL), a phenol-β2-agonist, has a very low residual content in the environment. Here, we present an ultrasensitive complete antigen-bridged PCR assay for detecting salbutamol (SAL). These DNA probes modified SAL complete antigens target recognition SAL antibodies and agglutinate synthetic DNA conjugates, thus enabling ligation of DNA probes to form a full-length DNA amplicon that contained a recognition site for cleavage endonuclease and subsequent quantification by qPCR.
Moreover, SAL antibodies were modified with magnetic beads which were used to reduce the background noise and sample matrix effect, and the DNA signals were isothermally amplified by strand displacement amplification technology. Some key parameters which influence assay performance were optimized: the length of the bridge oligonucleotide, the concentration of immunomagnetic beads, SAL probes, and initiation chain, etc.
Under the optimum conditions, the signal amplification of proposed Immuno-PCR assay for the detection of SAL was exponential, resulting in high potential sensitivity(~1 fg/mL) and a broad detection dynamic range (> 105 fold). Using this proposed method, we detected SAL in spiked tap water and urine samples with acceptable recoveries ranging from 88.1 to 103.3%. Theoretically, the method developed here has broad applicability and practical utility in immunoassays of a wide variety of analytes.

Application of Quantitative PCR in the Diagnosis and Evaluating Treatment Efficacy of Leishmaniasis

Leishmaniasis is still a serious neglected tropical disease that may cause death in infected individuals. At present, the clinical diagnosis and treatment monitoring still rely on parasitological culture and microscopy that needs experienced technicians. The low sensitivity and inconvenience of microscopic examination could cause misdiagnosis and relapse of leishmaniasis.
There is an urgent need for developing a sensitive and easily operated diagnostic method for the diagnosis and disease management of leishmaniasis. Thus, a quantitative real-time PCR (qPCR) based on the conversed regions of kinetoplast minicircle DNA (mkDNA) of Leishmania spp. was developed to detect different species of Leishmania.
The designed mkDNA-based qPCR was able to detect as low as one copy of Leishmania mkDNA or DNA from single parasite. It also detected Pan-Leishmania protozoa including Leishmania donovani, Leishmania infantum and Leishmania major without cross-reaction with other pathogen DNAs available in our lab.
This method was clinically applied to quantitatively detect skin lesion samples from 20 cutaneous leishmaniasis (CL) and bone marrow and/or PBMC samples from 30 current and cured visceral leishmaniasis (VL) patients, and blood samples from 11 patients with other infections and 5 normal donors as well.
Total 20 skin lesion samples from current CL patients and 20 bone marrow and/or PBMC samples from current VL patients were all detected as positive with qPCR without cross-reaction with samples from patients with malaria, brucellosis and dengue or normal donors. Two VL patients with parasite converted to microscopically negative after treatment were detected positive with qPCR.
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Relaxin 3 Polyclonal Antibody

ABP53672-003ml 0.03ml
EUR 158
Description: A polyclonal antibody for detection of Relaxin 3 from Human. This Relaxin 3 antibody is for IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human Relaxin 3

Relaxin 3 Polyclonal Antibody

ABP53672-01ml 0.1ml
EUR 289
Description: A polyclonal antibody for detection of Relaxin 3 from Human. This Relaxin 3 antibody is for IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human Relaxin 3

Relaxin 3 Polyclonal Antibody

ABP53672-02ml 0.2ml
EUR 414
Description: A polyclonal antibody for detection of Relaxin 3 from Human. This Relaxin 3 antibody is for IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human Relaxin 3

Relaxin 3 (RLN3) Antibody

20-abx323035
  • EUR 314.00
  • EUR 244.00
  • 100 ug
  • 50 ug

Relaxin 3 (RLN3) Antibody

20-abx218313
  • EUR 425.00
  • EUR 342.00
  • 100 ug
  • 50 ug

Relaxin 3 Blocking Peptide

20-abx161415
  • EUR 606.00
  • EUR 1428.00
  • 1 mg
  • 5 mg

Relaxin 3 (RLN3) Antibody

20-abx178243
  • EUR 1233.00
  • EUR 592.00
  • 1 mg
  • 200 ug

Relaxin 3 (RLN3) Antibody

20-abx178244
  • EUR 1233.00
  • EUR 592.00
  • 1 mg
  • 200 ug

Relaxin 3 (RLN3) Antibody

20-abx174368
  • EUR 356.00
  • EUR 913.00
  • EUR 467.00
  • EUR 154.00
  • EUR 272.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Anti-Relaxin 3 antibody

STJ95406 200 µl
EUR 197
Description: Rabbit polyclonal to Relaxin 3.

Recombinant Relaxin 3 (RLN3)

4-RPD869Ra01
  • EUR 490.66
  • EUR 234.00
  • EUR 1564.96
  • EUR 588.32
  • EUR 1076.64
  • EUR 391.00
  • EUR 3762.40
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Rat Relaxin 3 expressed in: E.coli

Relaxin-3 Recombinant Protein

40-230-0005mg 0.005 mg
EUR 259.25
Description: Relaxin-3 (H3 relaxin, Insulin-like peptide-7, INSL7) is a secreted protein structurally related to insulin, which is expressed primarily in the brain and central nervous system. Relaxin-3 has been identified as the ligand for the GPCR135 receptor, previously known as "somatostatin-like” or "angiotensin-like” peptide receptor, and also binds specifically to the LGR7 receptor, previously identified as an "orphan” G protein coupled receptor. Signaling by Relaxin-3 through its target receptors is, most likely, part of a CNS processing system, activated in response to signaling by neuropeptides and other factors. Intracerebroventricular injections of Relaxin-3 have been shown to cause a significant increase of food intake and body weight in Wistar rats. Recombinant Relaxin-3 is a 5.5 kDa disulfide linked heterodimeric protein consisting of a 24 amino acid A-chain and a 27 amino acid B-chain.

Relaxin-3 Recombinant Protein

40-230-0025mg 0.025 mg
EUR 364.25
Description: Relaxin-3 (H3 relaxin, Insulin-like peptide-7, INSL7) is a secreted protein structurally related to insulin, which is expressed primarily in the brain and central nervous system. Relaxin-3 has been identified as the ligand for the GPCR135 receptor, previously known as "somatostatin-like” or "angiotensin-like” peptide receptor, and also binds specifically to the LGR7 receptor, previously identified as an "orphan” G protein coupled receptor. Signaling by Relaxin-3 through its target receptors is, most likely, part of a CNS processing system, activated in response to signaling by neuropeptides and other factors. Intracerebroventricular injections of Relaxin-3 have been shown to cause a significant increase of food intake and body weight in Wistar rats. Recombinant Relaxin-3 is a 5.5 kDa disulfide linked heterodimeric protein consisting of a 24 amino acid A-chain and a 27 amino acid B-chain.

Relaxin H2 (human)

H-6784.0200 0.2mg
EUR 618
Description: Sum Formula: C256H408N74O74S8; CAS# [99489-94-8] net

Relaxin H2 (human)

H-6784.1000 1.0mg
EUR 2378
Description: Sum Formula: C256H408N74O74S8; CAS# [99489-94-8] net

Relaxin H3 (human)

H-7322.0200 0.2mg
EUR 618
Description: Sum Formula: C237H374N70O69S6; CAS# [1158181-62-4] net

Relaxin H3 (human)

H-7322.1000 1.0mg
EUR 2378
Description: Sum Formula: C237H374N70O69S6; CAS# [1158181-62-4] net

Human Relaxin 3 (RLN3) ELISA Kit

abx512123-96tests 96 tests
EUR 754

Human RLN3/ Relaxin-3 ELISA Kit

E2158Hu 1 Kit
EUR 571

Human Relaxin 3 (RLN3) ELISA Kit

abx253713-96tests 96 tests
EUR 754

RLN3 Relaxin-3 Human Recombinant Protein

PROTQ8WXF3 Regular: 25ug
EUR 317
Description: Relaxin-3 Human Recombinant produced in E.Coli is a disulfide-linked heterodimeric, non-glycosylated, polypeptide chain containing 24 amino acids for A chain and 27 amino acids for B chain and having a molecular mass of 2.5kDa for A chain and 3kDa for B chain.;The Relaxin-3 is purified by proprietary chromatographic techniques.

ELISA kit for Human Relaxin 3

EK5653 96 tests
EUR 553
Description: Enzyme-linked immunosorbent assay kit for quantification of Human Relaxin 3 in samples from serum, plasma, tissue homogenates and other biological fluids.

Human Relaxin 3(RLN3)ELISA Kit

QY-E01376 96T
EUR 361

Human RLN3(Relaxin-3) ELISA Kit

EH0755 96T
EUR 567.6
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml

Relaxin Receptor 3 Polyclonal Antibody

ES6513-100ul 100ul
EUR 279
Description: A Rabbit Polyclonal antibody against Relaxin Receptor 3 from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, WB, ELISA

Relaxin Receptor 3 Polyclonal Antibody

ES6513-50ul 50ul
EUR 207
Description: A Rabbit Polyclonal antibody against Relaxin Receptor 3 from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, WB, ELISA

Relaxin Receptor 3 Polyclonal Antibody

ABP55514-003ml 0.03ml
EUR 158
Description: A polyclonal antibody for detection of Relaxin Receptor 3 from Human, Mouse, Rat. This Relaxin Receptor 3 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human Relaxin Receptor 3 at AA range: 130-210

Relaxin Receptor 3 Polyclonal Antibody

ABP55514-01ml 0.1ml
EUR 289
Description: A polyclonal antibody for detection of Relaxin Receptor 3 from Human, Mouse, Rat. This Relaxin Receptor 3 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human Relaxin Receptor 3 at AA range: 130-210

Relaxin Receptor 3 Polyclonal Antibody

ABP55514-02ml 0.2ml
EUR 414
Description: A polyclonal antibody for detection of Relaxin Receptor 3 from Human, Mouse, Rat. This Relaxin Receptor 3 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human Relaxin Receptor 3 at AA range: 130-210

Relaxin 3 receptor 1 Antibody

abx019161-100ug 100 ug
EUR 342

Rat Relaxin 3 (RLN3) Protein

20-abx168490
  • EUR 690.00
  • EUR 286.00
  • EUR 2110.00
  • EUR 815.00
  • EUR 495.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Anti-Relaxin Receptor 3 antibody

STJ95408 200 µl
EUR 197
Description: Rabbit polyclonal to Relaxin Receptor 3.

Mouse Relaxin 3 (RLN3) Protein

20-abx654935
  • EUR 578.00
  • EUR 258.00
  • EUR 1720.00
  • EUR 690.00
  • EUR 425.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Mouse Relaxin 3 (RLN3) Protein

20-abx654936
  • EUR 578.00
  • EUR 258.00
  • EUR 1720.00
  • EUR 690.00
  • EUR 425.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Relaxin 3 receptor 1 Antibody

48026-100ul 100ul
EUR 333

Relaxin 3 receptor 1 Antibody

48026-50ul 50ul
EUR 239

ELISA kit for Human RLN3 (Relaxin-3)

E-EL-H1652 1 plate of 96 wells
EUR 534
Description: A sandwich ELISA kit for quantitative measurement of Human RLN3 (Relaxin-3) in samples from Serum, Plasma, Cell supernatant

ELISA kit for Human Relaxin-3 (RLN3)

KTE60790-48T 48T
EUR 332
Description: Quantitative sandwich ELISA for measuring Human Relaxin-3 (RLN3) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human Relaxin-3 (RLN3)

KTE60790-5platesof96wells 5 plates of 96 wells
EUR 2115
Description: Quantitative sandwich ELISA for measuring Human Relaxin-3 (RLN3) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human Relaxin-3 (RLN3)

KTE60790-96T 96T
EUR 539
Description: Quantitative sandwich ELISA for measuring Human Relaxin-3 (RLN3) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

Human Relaxin (RLN) Protein

20-abx654931
  • EUR 578.00
  • EUR 258.00
  • EUR 1720.00
  • EUR 690.00
  • EUR 425.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Human Relaxin ELISA Kit

ELA-E1216h 96 Tests
EUR 824

Relaxin-2, human recombinant

7194-10
EUR 207

Relaxin-2, human recombinant

7194-50
EUR 675

Human Relaxin ELISA kit

E01R0008-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Human Relaxin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Relaxin ELISA kit

E01R0008-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Human Relaxin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Relaxin ELISA kit

E01R0008-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Human Relaxin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Porcine relaxin peptides / relaxin (RLN) ELISA Kit

QY-E40036 96T
EUR 400

Recombinant Human MMP-3 Protein

PROTP08254-3 10ug
EUR 317
Description: Matrix metalloproteinases (MMPs) are a family of endoproteases that require zinc and calcium for expressing catalytic activity. These enzymes play a central role in the maintenance and remodeling of the extracellular matrix. Elevated expression of their activity, caused either by up-regulation of their expression or down-regulation of their cognate inhibitors, has been implicated in various degenerative disorders, including arthritis, cardiovascular disease, skeletal growth-plate disorders, and cancer metastasis. MMP-3 degrades fibronectin, laminin, collagens III, IV, and X, and cartilage proteoglycans. Recombinant human MMP-3 is a 42.8 kDa protein containing the entire catalytic N-terminal domain and the C-terminal domain (378 amino acids).

IL-3 Interleukin-3 Human Recombinant Protein, His Tag

PROTP08700-3 Regular: 50ug
EUR 317
Description: Interleukin-3 Human Recombinant produced in E.Coli is single, a non-glycosylated, Polypeptide chain containing 154 amino acids fragment (20-152) and having a total molecular mass of 17.3kDa and fused with a 20 aa N-terminal His tag. ;The IL3 His is purified by proprietary chromatographic techniques.

Mouse Relaxin 3 (RLN3) ELISA Kit

20-abx154612
  • EUR 7378.00
  • EUR 3933.00
  • EUR 911.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Rat Relaxin 3 (RLN3) ELISA Kit

abx575706-96tests 96 tests
EUR 668

Mouse Relaxin 3 (RLN3) ELISA Kit

abx576633-96tests 96 tests
EUR 668

Pig Relaxin 3 (RLN3) ELISA Kit

abx512125-96tests 96 tests
EUR 911

Mouse Rln3/ Relaxin-3 ELISA Kit

E1272Mo 1 Kit
EUR 571

Rat Rln3/ Relaxin-3 ELISA Kit

E0845Ra 1 Kit
EUR 571

Mouse Relaxin 3 (RLN3) CLIA Kit

20-abx490509
  • EUR 7973.00
  • EUR 4246.00
  • EUR 981.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Rat Relaxin 3 (RLN3) CLIA Kit

20-abx490510
  • EUR 8569.00
  • EUR 4560.00
  • EUR 1052.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Rat Relaxin 3 (RLN3) ELISA Kit

abx256229-96tests 96 tests
EUR 746

Mouse Relaxin 3 (RLN3) ELISA Kit

abx254703-96tests 96 tests
EUR 746

Rat Relaxin 3 (RLN3) ELISA Kit

20-abx156042
  • EUR 7237.00
  • EUR 3855.00
  • EUR 895.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Mouse Relaxin 3 (RLN3) ELISA Kit

RDR-RLN3-Mu-48Tests 48 Tests
EUR 557

Mouse Relaxin 3 (RLN3) ELISA Kit

RDR-RLN3-Mu-96Tests 96 Tests
EUR 774

Rat Relaxin 3 (RLN3) ELISA Kit

RDR-RLN3-Ra-48Tests 48 Tests
EUR 583

Rat Relaxin 3 (RLN3) ELISA Kit

RDR-RLN3-Ra-96Tests 96 Tests
EUR 811

Mouse Relaxin 3 (RLN3) ELISA Kit

RD-RLN3-Mu-48Tests 48 Tests
EUR 533

Mouse Relaxin 3 (RLN3) ELISA Kit

RD-RLN3-Mu-96Tests 96 Tests
EUR 740

Rat Relaxin 3 (RLN3) ELISA Kit

RD-RLN3-Ra-48Tests 48 Tests
EUR 557

Rat Relaxin 3 (RLN3) ELISA Kit

RD-RLN3-Ra-96Tests 96 Tests
EUR 775

ELISA kit for Rat Relaxin-3

EK1459 96 tests
EUR 553
Description: Enzyme-linked immunosorbent assay kit for quantification of Rat Relaxin-3 in samples from serum, plasma, tissue homogenates and other biological fluids.

ELISA kit for Mouse Relaxin-3

EK1460 96 tests
EUR 553
Description: Enzyme-linked immunosorbent assay kit for quantification of Mouse Relaxin-3 in samples from serum, plasma, tissue homogenates and other biological fluids.

Rat Rln3(Relaxin-3) ELISA Kit

ER0251 96T
EUR 567.6
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Rattus;Sensitivity: 46.9pg/ml

Mouse Rln3(Relaxin-3) ELISA Kit

EM0347 96T
EUR 567.6
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Mus ;Sensitivity: 0.938mIU/ml

Mouse Relaxin 3 (RLN3) ELISA Kit

DLR-RLN3-Mu-48T 48T
EUR 527
Description: A competitive inhibition quantitative ELISA assay kit for detection of Mouse Relaxin 3 (RLN3) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Mouse Relaxin 3 (RLN3) ELISA Kit

DLR-RLN3-Mu-96T 96T
EUR 688
Description: A competitive inhibition quantitative ELISA assay kit for detection of Mouse Relaxin 3 (RLN3) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Rat Relaxin 3 (RLN3) ELISA Kit

DLR-RLN3-Ra-48T 48T
EUR 549
Description: A sandwich quantitative ELISA assay kit for detection of Rat Relaxin 3 (RLN3) in samples from serum, plasma or other biological fluids.

Rat Relaxin 3 (RLN3) ELISA Kit

DLR-RLN3-Ra-96T 96T
EUR 718
Description: A sandwich quantitative ELISA assay kit for detection of Rat Relaxin 3 (RLN3) in samples from serum, plasma or other biological fluids.

Relaxin 3 (RLN3) Monoclonal Antibody (Rat)

4-MAD869Ra21
  • EUR 268.00
  • EUR 2840.00
  • EUR 700.00
  • EUR 340.00
  • EUR 223.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Mouse monoclonal antibody against Rat Relaxin 3 (RLN3)

Mouse Relaxin 3 (RLN3) ELISA Kit

CED869Mu-10x96wellstestplate 10x96-wells test plate
EUR 4862.4
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Mouse Relaxin 3 (RLN3) in serum, plasma, tissue homogenates and other biological fluids.

Mouse Relaxin 3 (RLN3) ELISA Kit

CED869Mu-1x48wellstestplate 1x48-wells test plate
EUR 488.08
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Mouse Relaxin 3 (RLN3) in serum, plasma, tissue homogenates and other biological fluids.

Mouse Relaxin 3 (RLN3) ELISA Kit

CED869Mu-1x96wellstestplate 1x96-wells test plate
EUR 654.4
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Mouse Relaxin 3 (RLN3) in serum, plasma, tissue homogenates and other biological fluids.

Mouse Relaxin 3 (RLN3) ELISA Kit

CED869Mu-5x96wellstestplate 5x96-wells test plate
EUR 2644.8
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Mouse Relaxin 3 (RLN3) in serum, plasma, tissue homogenates and other biological fluids.

Mouse Relaxin 3 (RLN3) ELISA Kit

4-CED869Mu
  • EUR 4913.00
  • EUR 2595.00
  • EUR 655.00
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Competitive Inhibition method for detection of Mouse Relaxin 3 (RLN3) in samples from Serum, plasma, tissue homogenates and other biological fluids. with no significant corss-reactivity with analogues from other species.

Rat Relaxin 3 (RLN3) ELISA Kit

CED869Ra-10x96wellstestplate 10x96-wells test plate
EUR 5124.2
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Rat Relaxin 3 (RLN3) in serum, plasma and other biological fluids.

Rat Relaxin 3 (RLN3) ELISA Kit

CED869Ra-1x48wellstestplate 1x48-wells test plate
EUR 509.64
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Rat Relaxin 3 (RLN3) in serum, plasma and other biological fluids.

Rat Relaxin 3 (RLN3) ELISA Kit

CED869Ra-1x96wellstestplate 1x96-wells test plate
EUR 685.2
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Rat Relaxin 3 (RLN3) in serum, plasma and other biological fluids.

Rat Relaxin 3 (RLN3) ELISA Kit

CED869Ra-5x96wellstestplate 5x96-wells test plate
EUR 2783.4
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Rat Relaxin 3 (RLN3) in serum, plasma and other biological fluids.

Rat Relaxin 3 (RLN3) ELISA Kit

4-CED869Ra
  • EUR 5175.00
  • EUR 2734.00
  • EUR 686.00
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Competitive Inhibition method for detection of Rat Relaxin 3 (RLN3) in samples from Serum, plasma and other biological fluids. with no significant corss-reactivity with analogues from other species.

Relaxin 3 (RLN3) Polyclonal Antibody (Rat)

4-PAD869Ra01
  • EUR 259.00
  • EUR 2708.00
  • EUR 670.00
  • EUR 328.00
  • EUR 219.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Rat Relaxin 3 (RLN3)

Human Relaxin- 3 receptor 2, RXFP4 ELISA KIT

ELI-14493h 96 Tests
EUR 824

Human Relaxin- 3 receptor 1, RXFP3 ELISA KIT

ELI-22043h 96 Tests
EUR 824

FSH (Human Follicle-stimulating hormone) ELISA test

3 96T/Box Ask for price
Description: ELISA based test for quantitative detection of FSH (Human Follicle-stimulating hormone)
The patients with bigger skin lesion in CL and higher level of immunoglobulin or splenomegaly in VL, had the higher parasite load detected by qPCR. The parasite load was significantly reduced after treatment. In conclusion, the mkDNA-based qPCR assay that we developed in this study can be used not only for diagnosis of both cutaneous and visceral leishmaniasis with high sensitivity and specificity, but also for evaluating the severity and treatment efficacy of this disease, presenting a rapid and accurate tool for clinical surveillance, treatment monitoring and the end point determination of leishmaniasis.

Molecular Detection of the mcr Genes by Multiplex PCR

Background: The emergence and prevalence of plasmid-mediated colistin-resistant bacterial strains in recent years have raised great concerns in clinical medicine. It is urgently needed to develop a cheaper, faster, simpler, sensitive, and specific molecular detection method to identify and monitor the dissemination of the transferable resistant determinants.
Methods and results: Herein, eight pairs of primers were designed to set up a multiplex PCR method for the rapid and efficient determination of reported mcr genes. This assay can give results within 85 min (35 min for amplification and 50 min for electrophoresis). We validated the feasibility of this assay by testing the presence of mcr genes in 60 colistin-resistant isolates.
Conclusion: Our multiplex PCR technique exhibits remarkable advantages in the light of clear identification, efficiency of amplification, as well as the time consuming for detection, and thus could be useful for the surveillance and epidemiological research of plasmid-mediated colistin resistance, particularly for the under-resourced laboratories.

Use of the PCR in a Combined Methodological Approach for the Study of Human Fascioliasis in an Endemic Area

 

Purpose: Fascioliasis is a worldwide distributed trematodiasis considered a neglected disease. Diagnosis in humans has been traditionally based on parasitological and immunological techniques. Recently we reported the use of the PCR in stool samples for the individual diagnosis. The purpose of this study was to evaluate human fascioliasis by a combination of diagnostic methods in an area where the disease is highly endemic in animals.
Methods: We studied all the inhabitants (N = 240) of Tatón village, Argentina, by Fasciola hepatica rproCL1-ELISA. Among them, we continued the study with 13 cases that had at least two positive serological tests, who performed a questionnaire, physical examination, abdominal ultrasonography, and collection of blood and faeces. Blood/serum samples were used for Fh rproCL1-ELISA and liver function tests. Faeces were used for parasitological analysis and PCR of a repetitive fragment of Fasciola sp.
Results: Among the 13 patients, 9 presented symptoms of biliary colic. All patients repeated positive serology. F. hepatica eggs were not detected. PCR was positive in 11 cases.
Conclusion: This is the first report employing an approach based on the combination of methods for the evaluation of human fascioliasis in an endemic area, which includes molecular tools with a high value in detecting low infections.

Evaluation of a real-time PCR assay for diagnosis of schistosomiasis japonica in the domestic goat

 

Background: Schistosomiasis japonica is an infectious disease caused by Schistosoma japonicum that seriously endangers human health. Domestic animals have important roles in disease transmission and goats are considered a primary reservoir host and source of infection.
The prevalence and intensity of schistosomiasis infections have significantly decreased in China, and a more sensitive, specific detection method is urgently needed. The aim of this study was to develop a real-time PCR assay for accurate detection of S. japonicum infection in goats.
Methods: A real-time PCR method for detecting schistosomiasis japonica in goats was developed by amplification of a specific S. japonicum DNA fragment, and validated using a total of 94 negative and 159 positive plasma and serum samples collected in our previous study of S. japonicum infection.
Both plasma and serum samples were evaluated by real-time PCR and enzyme-linked immunosorbent assay (ELISA). In addition, 120 goat plasma samples from an S. japonicum-endemic area (Wangjiang) and 33 from a non-endemic region (Weihai) were collected and evaluated using our method.
Results: The sensitivity and specificity of the real-time PCR for detecting infected samples were 98.74% (157/159, 95% CI: 95.53-99.85%) and 100% (94/94, 95% CI: 96.15-100%), respectively. For the ELISA, sensitivity and specificity were 98.11% (156/159, 95% CI: 94.59-99.61%) and 90.43% (85/94, 95% CI: 82.60-95.53%), respectively. Further, we found positivity rates for S. japonicum infection in Wangjiang and Weihai of 8.33% (10/120, 95% CI: 4.07-14.79%) and 0% (0/33, 95% CI: 0-10.58%), respectively.
Conclusions: The results of this study indicate that our real-time PCR method exhibits higher sensitivity and specificity than ELISA and is a useful method for detection of S. japonicum infection in goats.

Gpr160/ Rat Gpr160 ELISA Kit

ELI-27265r 96 Tests
EUR 886

GPR160 Polyclonal Antibody

ES5525-100ul 100ul
EUR 279
Description: A Rabbit Polyclonal antibody against GPR160 from Human. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA

GPR160 Polyclonal Antibody

ES5525-50ul 50ul
EUR 207
Description: A Rabbit Polyclonal antibody against GPR160 from Human. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA

GPR160 Polyclonal Antibody

ABP54526-003ml 0.03ml
EUR 158
Description: A polyclonal antibody for detection of GPR160 from Human. This GPR160 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human GPR160 at AA rangle: 270-350

GPR160 Polyclonal Antibody

ABP54526-01ml 0.1ml
EUR 289
Description: A polyclonal antibody for detection of GPR160 from Human. This GPR160 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human GPR160 at AA rangle: 270-350

GPR160 Polyclonal Antibody

ABP54526-02ml 0.2ml
EUR 414
Description: A polyclonal antibody for detection of GPR160 from Human. This GPR160 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human GPR160 at AA rangle: 270-350

GPR160 Conjugated Antibody

C47605 100ul
EUR 397

GPR160 siRNA

20-abx918471
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol

GPR160 siRNA

20-abx918472
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol

GPR160 siRNA

20-abx902275
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol

Polyclonal GPR160 Antibody (Internal)

APR16513G 0.05ml
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human GPR160 (Internal). This antibody is tested and proven to work in the following applications:

GPR160 Blocking Peptide

20-abx063814
  • EUR 272.00
  • EUR 411.00
  • 1 mg
  • 5 mg

GPR160 Blocking Peptide

DF4899-BP 1mg
EUR 195

pCMV-SPORT6-GPR160

PVT12413 2 ug
EUR 391

Polyclonal GPR160 Antibody (Extracellular Domain)

APR16512G 0.05mg
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human GPR160 (Extracellular Domain). This antibody is tested and proven to work in the following applications:

Polyclonal Goat anti-GST α-form

GST-ANTI-1 50 uL
EUR 280

Polyclonal Goat anti-GST μ-form

GST-ANTI-2 50 uL
EUR 280

Polyclonal Goat anti-GST p-form

GST-ANTI-3 50 uL
EUR 280

Rat GPR160 shRNA Plasmid

20-abx990910
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg

Mouse GPR160 shRNA Plasmid

20-abx977549
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg

Human GPR160 shRNA Plasmid

20-abx958914
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg

pECMV-3-FLAG-GPR160

PVT12978 2 ug
EUR 703

GPR160 Recombinant Protein (Rat)

RP203351 100 ug Ask for price

GPR160 Recombinant Protein (Human)

RP061095 100 ug Ask for price

GPR160 Recombinant Protein (Mouse)

RP139520 100 ug Ask for price

GPR160 Recombinant Protein (Mouse)

RP139523 100 ug Ask for price

GPR160 Recombinant Protein (Mouse)

RP139526 100 ug Ask for price

Polyclonal GPR160 Antibody - C-terminal region

APR16514G 0.05mg
EUR 528
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human GPR160 - C-terminal region. This antibody is tested and proven to work in the following applications:

G Protein-Coupled Receptor 160 (GPR160) Antibody

20-abx324785
  • EUR 314.00
  • EUR 244.00
  • 100 ug
  • 50 ug

Gpr160 ORF Vector (Mouse) (pORF)

ORF046508 1.0 ug DNA
EUR 506

Gpr160 ORF Vector (Mouse) (pORF)

ORF046509 1.0 ug DNA
EUR 506

Gpr160 ORF Vector (Mouse) (pORF)

ORF046510 1.0 ug DNA
EUR 506

GPR160 ORF Vector (Human) (pORF)

ORF020366 1.0 ug DNA
EUR 405

Gpr160 ORF Vector (Rat) (pORF)

ORF067785 1.0 ug DNA
EUR 506

Probable G-Protein Coupled Receptor 160 (GPR160) Antibody

20-abx008198
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul

GPR160 sgRNA CRISPR Lentivector set (Human)

K0898801 3 x 1.0 ug
EUR 339

Gpr160 sgRNA CRISPR Lentivector set (Mouse)

K4464401 3 x 1.0 ug
EUR 339

Gpr160 sgRNA CRISPR Lentivector set (Rat)

K6604401 3 x 1.0 ug
EUR 339

GPR160 sgRNA CRISPR Lentivector (Human) (Target 1)

K0898802 1.0 ug DNA
EUR 154

GPR160 sgRNA CRISPR Lentivector (Human) (Target 2)

K0898803 1.0 ug DNA
EUR 154

GPR160 sgRNA CRISPR Lentivector (Human) (Target 3)

K0898804 1.0 ug DNA
EUR 154

Gpr160 sgRNA CRISPR Lentivector (Mouse) (Target 1)

K4464402 1.0 ug DNA
EUR 154

Gpr160 sgRNA CRISPR Lentivector (Mouse) (Target 2)

K4464403 1.0 ug DNA
EUR 154

Gpr160 sgRNA CRISPR Lentivector (Mouse) (Target 3)

K4464404 1.0 ug DNA
EUR 154

Gpr160 sgRNA CRISPR Lentivector (Rat) (Target 1)

K6604402 1.0 ug DNA
EUR 154

Gpr160 sgRNA CRISPR Lentivector (Rat) (Target 2)

K6604403 1.0 ug DNA
EUR 154

Gpr160 sgRNA CRISPR Lentivector (Rat) (Target 3)

K6604404 1.0 ug DNA
EUR 154

GPR160 Protein Vector (Human) (pPB-C-His)

PV081461 500 ng
EUR 552

GPR160 Protein Vector (Human) (pPB-N-His)

PV081462 500 ng
EUR 552

GPR160 Protein Vector (Human) (pPM-C-HA)

PV081463 500 ng
EUR 552

GPR160 Protein Vector (Human) (pPM-C-His)

PV081464 500 ng
EUR 552

GPR160 Protein Vector (Rat) (pPB-C-His)

PV271138 500 ng
EUR 603

GPR160 Protein Vector (Rat) (pPB-N-His)

PV271139 500 ng
EUR 603

GPR160 Protein Vector (Rat) (pPM-C-HA)

PV271140 500 ng
EUR 603

GPR160 Protein Vector (Rat) (pPM-C-His)

PV271141 500 ng
EUR 603

GPR160 Protein Vector (Mouse) (pPB-C-His)

PV186030 500 ng
EUR 603

GPR160 Protein Vector (Mouse) (pPB-N-His)

PV186031 500 ng
EUR 603

GPR160 Protein Vector (Mouse) (pPM-C-HA)

PV186032 500 ng
EUR 603

GPR160 Protein Vector (Mouse) (pPM-C-His)

PV186033 500 ng
EUR 603

GPR160 Protein Vector (Mouse) (pPB-C-His)

PV186034 500 ng
EUR 603

GPR160 Protein Vector (Mouse) (pPB-N-His)

PV186035 500 ng
EUR 603

GPR160 Protein Vector (Mouse) (pPM-C-HA)

PV186036 500 ng
EUR 603

GPR160 Protein Vector (Mouse) (pPM-C-His)

PV186037 500 ng
EUR 603

GPR160 Protein Vector (Mouse) (pPB-C-His)

PV186038 500 ng
EUR 603

GPR160 Protein Vector (Mouse) (pPB-N-His)

PV186039 500 ng
EUR 603

GPR160 Protein Vector (Mouse) (pPM-C-HA)

PV186040 500 ng
EUR 603

GPR160 Protein Vector (Mouse) (pPM-C-His)

PV186041 500 ng
EUR 603

GPR160 3'UTR GFP Stable Cell Line

TU059240 1.0 ml
EUR 1394

GPR160 3'UTR Luciferase Stable Cell Line

TU009240 1.0 ml
EUR 1394

Gpr160 3'UTR GFP Stable Cell Line

TU255349 1.0 ml Ask for price

Gpr160 3'UTR Luciferase Stable Cell Line

TU205349 1.0 ml Ask for price

Gpr160 3'UTR GFP Stable Cell Line

TU158996 1.0 ml Ask for price

Gpr160 3'UTR Luciferase Stable Cell Line

TU108996 1.0 ml Ask for price

GPR160 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV)

LV683023 1.0 ug DNA
EUR 682

GPR160 Lentiviral Vector (Rat) (UbC) (pLenti-GIII-UbC)

LV683027 1.0 ug DNA
EUR 682

GPR160 Lentiviral Vector (Rat) (EF1a) (pLenti-GIII-EF1a)

LV683028 1.0 ug DNA
EUR 682

Mouse Probable G- protein coupled receptor 160, Gpr160 ELISA KIT

ELI-48533m 96 Tests
EUR 865

Human Probable G- protein coupled receptor 160, GPR160 ELISA KIT

ELI-08752h 96 Tests
EUR 824

GPR160 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Human)

K0898805 3 x 1.0 ug
EUR 376

Gpr160 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Mouse)

K4464405 3 x 1.0 ug
EUR 376

Gpr160 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Rat)

K6604405 3 x 1.0 ug
EUR 376

GPR160 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 1)

K0898806 1.0 ug DNA
EUR 167

GPR160 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 2)

K0898807 1.0 ug DNA
EUR 167

GPR160 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 3)

K0898808 1.0 ug DNA
EUR 167

GPR160 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-C-term-HA)

LV683024 1.0 ug DNA
EUR 682

GPR160 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-GFP-2A-Puro)

LV683025 1.0 ug DNA
EUR 740

GPR160 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-RFP-2A-Puro)

LV683026 1.0 ug DNA
EUR 740

Gpr160 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 1)

K4464406 1.0 ug DNA
EUR 167

Gpr160 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 2)

K4464407 1.0 ug DNA
EUR 167

Gpr160 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 3)

K4464408 1.0 ug DNA
EUR 167