Molecular Detection of the mcr Genes by Multiplex PCR

November 12, 2020
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Complete antigen-bridged DNA strand displacement amplification immuno-PCR assay for ultrasensitive detection of salbutamol

 

Monitoring of low-level analytes are typical examples for analytical challenges. Salbutamol (SAL), a phenol-β2-agonist, has a very low residual content in the environment. Here, we present an ultrasensitive complete antigen-bridged PCR assay for detecting salbutamol (SAL). These DNA probes modified SAL complete antigens target recognition SAL antibodies and agglutinate synthetic DNA conjugates, thus enabling ligation of DNA probes to form a full-length DNA amplicon that contained a recognition site for cleavage endonuclease and subsequent quantification by qPCR.
Moreover, SAL antibodies were modified with magnetic beads which were used to reduce the background noise and sample matrix effect, and the DNA signals were isothermally amplified by strand displacement amplification technology. Some key parameters which influence assay performance were optimized: the length of the bridge oligonucleotide, the concentration of immunomagnetic beads, SAL probes, and initiation chain, etc.
Under the optimum conditions, the signal amplification of proposed Immuno-PCR assay for the detection of SAL was exponential, resulting in high potential sensitivity(~1 fg/mL) and a broad detection dynamic range (> 105 fold). Using this proposed method, we detected SAL in spiked tap water and urine samples with acceptable recoveries ranging from 88.1 to 103.3%. Theoretically, the method developed here has broad applicability and practical utility in immunoassays of a wide variety of analytes.

Application of Quantitative PCR in the Diagnosis and Evaluating Treatment Efficacy of Leishmaniasis

Leishmaniasis is still a serious neglected tropical disease that may cause death in infected individuals. At present, the clinical diagnosis and treatment monitoring still rely on parasitological culture and microscopy that needs experienced technicians. The low sensitivity and inconvenience of microscopic examination could cause misdiagnosis and relapse of leishmaniasis.
There is an urgent need for developing a sensitive and easily operated diagnostic method for the diagnosis and disease management of leishmaniasis. Thus, a quantitative real-time PCR (qPCR) based on the conversed regions of kinetoplast minicircle DNA (mkDNA) of Leishmania spp. was developed to detect different species of Leishmania.
The designed mkDNA-based qPCR was able to detect as low as one copy of Leishmania mkDNA or DNA from single parasite. It also detected Pan-Leishmania protozoa including Leishmania donovani, Leishmania infantum and Leishmania major without cross-reaction with other pathogen DNAs available in our lab.
This method was clinically applied to quantitatively detect skin lesion samples from 20 cutaneous leishmaniasis (CL) and bone marrow and/or PBMC samples from 30 current and cured visceral leishmaniasis (VL) patients, and blood samples from 11 patients with other infections and 5 normal donors as well.
Total 20 skin lesion samples from current CL patients and 20 bone marrow and/or PBMC samples from current VL patients were all detected as positive with qPCR without cross-reaction with samples from patients with malaria, brucellosis and dengue or normal donors. Two VL patients with parasite converted to microscopically negative after treatment were detected positive with qPCR.
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Relaxin-3, human recombinant
7195-50 each
EUR 810

Relaxin-3 Human Recombinant
rAP-4650 Inquiry Ask for price

INSL7 / Relaxin 3 (Human)
035-36 20 μg
EUR 160.92

INSL7 / Relaxin 3 (Human)
035-36A 100 μg
EUR 604.8

Human Relaxin-3 ELISA Kit
MBS2887639-10x96StripWells 10x96-Strip-Wells
EUR 4080

Human Relaxin-3 ELISA Kit
MBS2887639-48StripWells 48-Strip-Wells
EUR 390

Human Relaxin-3 ELISA Kit
MBS2887639-5x96StripWells 5x96-Strip-Wells
EUR 2220

Human Relaxin-3 ELISA Kit
MBS2887639-96StripWells 96-Strip-Wells
EUR 520

Human Relaxin-3 ELISA Kit
MBS765502-10x96StripWells 10x96-Strip-Wells
EUR 3900

Human Relaxin-3 ELISA Kit
MBS765502-48StripWells 48-Strip-Wells
EUR 340

Human Relaxin-3 ELISA Kit
MBS765502-5x96StripWells 5x96-Strip-Wells
EUR 2045

Human Relaxin-3 ELISA Kit
MBS765502-96StripWells 96-Strip-Wells
EUR 455

Human Relaxin-3,RLN-3 ELISA
YLA4309HU-48T 48T Ask for price

Human Relaxin-3,RLN-3 ELISA
YLA4309HU-96T 96T Ask for price

Human Relaxin 3 ELISA Kit
MBS054925-10x96StripWells 10x96-Strip-Wells
EUR 6725

Human Relaxin 3 ELISA Kit
MBS054925-48StripWells 48-Strip-Wells
EUR 550

Human Relaxin 3 ELISA Kit
MBS054925-5x96StripWells 5x96-Strip-Wells
EUR 3420

Human Relaxin 3 ELISA Kit
MBS054925-96StripWells 96-Strip-Wells
EUR 765

Human Relaxin 3 ELISA Kit
EK5653 96 tests
EUR 599

Human Relaxin 3 ELISA Kit
MBS8248392-5x96Tests 5x96Tests
EUR 2880

Human Relaxin 3 ELISA Kit
MBS8248392-96Tests 96Tests
EUR 645

Human relaxin 3 ELISA Kit
SL2396Hu 96 Tests
EUR 468

Human Relaxin-3 Recombinant Protein
100-356 25 µg
EUR 196.35
Description: Relaxin-3 (H3 relaxin, Insulin-like peptide-7, INSL7) is a secreted protein structurally related to insulin, which is expressed primarily in the brain and central nervous system. Relaxin-3 has been identified as the ligand for the GPCR135 receptor, previously known as “somatostatin-like” or “angiotensin-like” peptide receptor, and also binds specifically to the LGR7 receptor, previously identified as an “orphan” G protein coupled receptor. Signaling by Relaxin-3 through its target receptors is, most likely, part of a CNS processing system, activated in response to signaling by neuropeptides and other factors. Intracerebroventricular injections of Relaxin-3 have been shown to cause a significant increase of food intake and body weight in Wistar rats. Recombinant Relaxin-3 is a 5.5 kDa disulfide linked heterodimeric protein consisting of a 24 amino acid A-chain and a 27 amino acid B-chain.

Human Relaxin-3 Recombinant Protein
100-356S 5 µg
EUR 92.4
Description: Relaxin-3 (H3 relaxin, Insulin-like peptide-7, INSL7) is a secreted protein structurally related to insulin, which is expressed primarily in the brain and central nervous system. Relaxin-3 has been identified as the ligand for the GPCR135 receptor, previously known as “somatostatin-like” or “angiotensin-like” peptide receptor, and also binds specifically to the LGR7 receptor, previously identified as an “orphan” G protein coupled receptor. Signaling by Relaxin-3 through its target receptors is, most likely, part of a CNS processing system, activated in response to signaling by neuropeptides and other factors. Intracerebroventricular injections of Relaxin-3 have been shown to cause a significant increase of food intake and body weight in Wistar rats. Recombinant Relaxin-3 is a 5.5 kDa disulfide linked heterodimeric protein consisting of a 24 amino acid A-chain and a 27 amino acid B-chain.

Recombinant Human Relaxin-3 Protein
PROTQ8WXF3-1 25ug
EUR 380.4
Description: Relaxin-3 (H3 relaxin, Insulin-like peptide-7, INSL7) is a secreted protein structurally related to insulin, which is expressed primarily in the brain and central nervous system. Relaxin-3 has been identified as the ligand for the GPCR135 receptor, previously known as “somatostatin-like” or “angiotensin-like” peptide receptor, and also binds specifically to the LGR7 receptor, previously identified as an “orphan” G protein coupled receptor. Signaling by Relaxin-3 through its target receptors is, most likely, part of a CNS processing system, activated in response to signaling by neuropeptides and other factors. Intracerebroventricular injections of Relaxin-3 have been shown to cause a significant increase of food intake and body weight in Wistar rats. Recombinant Relaxin-3 is a 5.5 kDa disulfide linked heterodimeric protein consisting of a 24 amino acid A-chain and a 27 amino acid B-chain.

Human Relaxin-1
hAP-0525 50ug
EUR 400

Human Relaxin-2
MBS691734-0005mg 0.005mg
EUR 300

Human Relaxin-2
MBS691734-0025mg 0.025mg
EUR 450

Human Relaxin-2
MBS691734-5x0025mg 5x0.025mg
EUR 1725

Human Relaxin 3 (RLN3) Protein
abx680520-100g 100 µg
EUR 225

ELISA Kit for Human Relaxin-3
E0189h 96T
EUR 387.4

Human Relaxin-3 (RLN3) ELISA Kit
AE22052HU-48T 48T
EUR 325
Description: Human (Homo sapiens)

Human Relaxin-3 (RLN3) ELISA Kit
AE22052HU-48Tests 48 Tests
EUR 325
Description: Human (Homo sapiens)

Human Relaxin-3 (RLN3) ELISA Kit
AE22052HU-96T 96T
EUR 610
Description: Human (Homo sapiens)

Human Relaxin-3 (RLN3) ELISA Kit
AE22052HU-96Tests 96 Tests
EUR 610
Description: Human (Homo sapiens)

Human Relaxin-3(RLN3) Elisa Kit
EK713553 96 Wells
EUR 0.37

Human Relaxin-3 (RLN3) ELISA Kit
EK7514 96Т
EUR 799

Human Relaxin-3 (RLN3) ELISA Kit
KTE60790-48T 48T
EUR 339
Description: This Human Relaxin-3 (RLN3) ELISA Kit employs a two-site sandwich ELISA to quantitate RLN3.

Human Relaxin-3 (RLN3) ELISA Kit
KTE60790-96T 96T
EUR 589
Description: This Human Relaxin-3 (RLN3) ELISA Kit employs a two-site sandwich ELISA to quantitate RLN3.

Human Relaxin-3 (RLN3) ELISA Kit
KTE60790-96T5 96 T×5
EUR 2629
Description: This Human Relaxin-3 (RLN3) ELISA Kit employs a two-site sandwich ELISA to quantitate RLN3.

Human Relaxin-3 (RLN3) ELISA Kit
KTE60790-96T50 96 T×50
EUR 24569
Description: This Human Relaxin-3 (RLN3) ELISA Kit employs a two-site sandwich ELISA to quantitate RLN3.

Human Relaxin-3 (RLN3) ELISA Kit
MBS9714455-48Tests 48Tests
EUR 340

Human Relaxin-3 (RLN3) ELISA Kit
MBS9714455-5x96Tests 5x96Tests
EUR 1820

Human Relaxin-3 (RLN3) ELISA Kit
MBS9714455-96Tests 96Tests
EUR 500

Human Relaxin-3 (RLN3) ELISA Kit
RK06347 96T
EUR 322.37

Relaxin-2 (Human)
MBS408061-005mg 0.05mg
EUR 345

Relaxin-2 (Human)
MBS408061-5x005mg 5x0.05mg
EUR 1500

Relaxin 3, Recombinant, Human (H3 Relaxin, Insulin-Like Peptide-7, INSL7)
MBS650130-0005mg 0.005mg
EUR 340

Relaxin 3, Recombinant, Human (H3 Relaxin, Insulin-Like Peptide-7, INSL7)
MBS650130-0025mg 0.025mg
EUR 490

Relaxin 3, Recombinant, Human (H3 Relaxin, Insulin-Like Peptide-7, INSL7)
MBS650130-5x0025mg 5x0.025mg
EUR 2055

Human RLN3/ Relaxin-3 ELISA Kit
E2158Hu 1 Kit
EUR 515
Description: UNQ6188/PRO20213,INSL7,RXN3,ZINS4,Insulin-like peptide INSL7,Prorelaxin H3,Insulin-like peptide 7

Human RLN3(Relaxin-3) ELISA Kit
EH0755 96T
EUR 681.12
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml

Human RLN3 (Relaxin-3) ELISA Kit
MBS2516020-10x96Tests 10x96Tests
EUR 4060

Human RLN3 (Relaxin-3) ELISA Kit
MBS2516020-24Tests 24Tests
EUR 265

Human RLN3 (Relaxin-3) ELISA Kit
MBS2516020-48Test 48Test
EUR 450

Human RLN3 (Relaxin-3) ELISA Kit
MBS2516020-5x96Test 5x96Test
EUR 2265

Human RLN3 (Relaxin-3) ELISA Kit
MBS2516020-96Tests 96Tests
EUR 535

Human RLN3(Relaxin-3) ELISA Kit
RE1961H-48wells 48 wells
EUR 125.55

Human RLN3(Relaxin-3) ELISA Kit
RE1961H-48wellsplate 48 wells plate
EUR 126

Human RLN3(Relaxin-3) ELISA Kit
RE1961H-96wells 96 wells
EUR 179.55

Human RLN3(Relaxin-3) ELISA Kit
RE1961H-96wellsplate 96 wells plate
EUR 180

INSL7 / Relaxin 3 (Human) - Antibody
H-035-36 50 μl
EUR 604.8

Human Relaxin 3 (RLN3) ELISA Kit
abx512123-96tests 96 tests
EUR 904.8

Human Relaxin 3 (RLN3) ELISA Kit
abx253713-96tests 96 tests
EUR 562.5

Human Relaxin 3,RLN3 ELISA KIT
JOT-EK7543Hu 96 wells Ask for price
Description: Human

Human Relaxin 3,RLN3 ELISA KIT
E7543Hu-1096T 10*96T
EUR 4122

Human Relaxin 3,RLN3 ELISA KIT
E7543Hu-48wells 48 wells
EUR 300

Human Relaxin 3,RLN3 ELISA KIT
E7543Hu-596T 5*96T
EUR 2061

Human Relaxin 3,RLN3 ELISA KIT
E7543Hu-96wells 96 wells
EUR 458

Human Relaxin 3, RLN3 ELISA Kit
MBS1609027-10x96StripWells 10x96-Strip-Wells
EUR 3460

Human Relaxin 3, RLN3 ELISA Kit
MBS1609027-48StripWells 48-Strip-Wells
EUR 285

Human Relaxin 3, RLN3 ELISA Kit
MBS1609027-5x96StripWells 5x96-Strip-Wells
EUR 1750

Human Relaxin 3, RLN3 ELISA Kit
MBS1609027-96StripWells 96-Strip-Wells
EUR 425

Nori® Human Relaxin-3 ELISA Kit
GR111288 96-well
EUR 461

Relaxin 2 (Human)
035-62 20 μg
EUR 261.36

Relaxin 2 (Human)
035-62A 100 μg
EUR 469.8

Relaxin H2 (human)
4039910.02 0.2 mg
EUR 455.07

Relaxin H2 (human)
4039910.1 1 mg
EUR 1818.92

Relaxin H3 (human)
4039569.02 0.2 mg
EUR 455.07

Relaxin H3 (human)
4039569.1 1 mg
EUR 1818.92

Relaxin H2 (human)
H-6784.0200 0.2mg
EUR 741.6
Description: Sum Formula: C256H408N74O74S8; CAS# [99489-94-8] net

Relaxin H2 (human)
H-6784.1000 1.0mg
EUR 2853.6
Description: Sum Formula: C256H408N74O74S8; CAS# [99489-94-8] net

Relaxin H3 (human)
H-7322.0200 0.2mg
EUR 741.6
Description: Sum Formula: C237H374N70O69S6; CAS# [1158181-62-4] net

Relaxin H3 (human)
H-7322.1000 1.0mg
EUR 2853.6
Description: Sum Formula: C237H374N70O69S6; CAS# [1158181-62-4] net

Recombinant Human Relaxin-3 (RLN3), partial
MBS7094488-005mgBaculovirus 0.05mg(Baculovirus)
EUR 885

Recombinant Human Relaxin-3 (RLN3), partial
MBS7094488-005mgEColi 0.05mg(E-Coli)
EUR 510

Recombinant Human Relaxin-3 (RLN3), partial
MBS7094488-02mgEColi 0.2mg(E-Coli)
EUR 665

Recombinant Human Relaxin-3 (RLN3), partial
MBS7094488-02mgYeast 0.2mg(Yeast)
EUR 945

Recombinant Human Relaxin-3 (RLN3), partial
MBS7094488-05mgEColi 0.5mg(E-Coli)
EUR 710

ELISA kit for Human Relaxin-3 (RLN3)
KTE60790-5platesof96wells 5 plates of 96 wells
EUR 2538
Description: Quantitative sandwich ELISA for measuring Human Relaxin-3 (RLN3) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

INSL7 / Relaxin 3 (Human) - RIA Kit
RK-035-36 125 tubes
EUR 1061.64

Relaxin-3
AP87248 1mg
EUR 2640

Relaxin-3
AP87370 1mg
EUR 2640

Relaxin-3
AP87734 1mg
EUR 2640

Relaxin-3
AP88315 1mg
EUR 2640

Human Relaxin 3-RLN3-ELISA Kit
QY-E01376 96T
EUR 400

Human RLN3 (Relaxin 3) ELISA Kit
MBS8819812-10x96StripWells 10x96-Strip-Wells
EUR 3130

Human RLN3 (Relaxin 3) ELISA Kit
MBS8819812-48StripWells 48-Strip-Wells
EUR 350

Human RLN3 (Relaxin 3) ELISA Kit
MBS8819812-5x96StripWells 5x96-Strip-Wells
EUR 1710

Human RLN3 (Relaxin 3) ELISA Kit
MBS8819812-96StripWells 96-Strip-Wells
EUR 445

Human Relaxin 3 PicoKine ELISA Kit
MBS177627-5x96StripWells 5x96-Strip-Wells
EUR 3935

Human Relaxin 3 PicoKine ELISA Kit
MBS177627-96StripWells 96-Strip-Wells
EUR 855

RLN3 (untagged)-Human relaxin 3 (RLN3)
SC305868 10 µg Ask for price

ELISA kit for Human RLN3 (Relaxin-3)
E-EL-H1652 1 plate of 96 wells
EUR 640.8
Description: A sandwich ELISA kit for quantitative measurement of Human RLN3 (Relaxin-3) in samples from Serum, Plasma, Cell supernatant

Relaxin 3 (BDelta23-27) R (Human)
036-20 100 μg
EUR 461.16

EasyPro Human Relaxin-3(RLN3)ELISA Kit
FY-EH1961S each
EUR 520
Description: 18.75 pg/mL

RLN3 Relaxin-3 Human Recombinant Protein
PROTQ8WXF3 Regular: 25ug
EUR 250
Description: Relaxin-3 Human Recombinant produced in E. coli is a disulfide-linked heterodimeric, non-glycosylated, polypeptide chain containing 24 amino acids for A chain and 27 amino acids for B chain and having a molecular mass of 2.5kDa for A chain and 3kDa for B chain. The Relaxin-3 is purified by proprietary chromatographic techniques.

INSL7 / Relaxin 3 (Human) - Cy5 Labeled
FC5-035-36 1 nmol
EUR 756

Human Relaxin-3 Recombinant Protein Lyophilized
IHURLXN3RLY5UG each
EUR 341
Description: Human Relaxin-3 Recombinant Protein Lyophilized

Human Relaxin-3 Recombinant Protein Lyophilized
MBS8433281-INQUIRE INQUIRE Ask for price

Recombinant Human Relaxin-2
MBS146516-0005mg 0.005mg
EUR 240

Recombinant Human Relaxin-2
MBS146516-0025mg 0.025mg
EUR 310

Recombinant Human Relaxin-2
MBS146516-1mg 1mg
EUR 2880

Recombinant Human Relaxin-2
MBS146516-5x1mg 5x1mg
EUR 12630

Recombinant Human Relaxin-3 receptor 1 (RXFP3)
MBS7029195-002mg 0.02mg
EUR 1810

Recombinant Human Relaxin-3 receptor 1 (RXFP3)
MBS7029195-01mg 0.1mg
EUR 2745

Recombinant Human Relaxin-3 receptor 1 (RXFP3)
MBS7029195-5x01mg 5x0.1mg
EUR 12310

Recombinant Human Relaxin-3 receptor 2 (RXFP4)
MBS7029197-002mg 0.02mg
EUR 1695

Recombinant Human Relaxin-3 receptor 2 (RXFP4)
MBS7029197-01mg 0.1mg
EUR 2650

Recombinant Human Relaxin-3 receptor 2 (RXFP4)
MBS7029197-5x01mg 5x0.1mg
EUR 11890

Human Relaxin ELISA kit
E01A7441 96T
EUR 700
Description: ELISA

Human Relaxin ELISA Kit
MBS009232-10x96StripWells 10x96-Strip-Wells
EUR 6725

Human Relaxin ELISA Kit
MBS009232-48StripWells 48-Strip-Wells
EUR 550

Human Relaxin ELISA Kit
MBS009232-5x96StripWells 5x96-Strip-Wells
EUR 3420

Human Relaxin ELISA Kit
MBS009232-96StripWells 96-Strip-Wells
EUR 765

Human Relaxin ELISA kit
E01R0008-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Human Relaxin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Relaxin ELISA kit
E01R0008-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Human Relaxin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Relaxin ELISA kit
E01R0008-48wellsplate 48 wells plate
EUR 280

Human Relaxin ELISA kit
E01R0008-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Human Relaxin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Relaxin ELISA kit
E01R0008-96wellsplate 96 wells plate
EUR 405

Human Relaxin ELISA Kit
ELA-E1216h 96 Tests
EUR 988.8

Human Relaxin ELISA Kit
IHURLNKT each
EUR 700
Description: Human Relaxin ELISA Kit

Human Relaxin ELISA Kit
MBS732658-10x96StripWells 10x96-Strip-Wells
EUR 5685

Human Relaxin ELISA Kit
MBS732658-48StripWells 48-Strip-Wells
EUR 485

Human Relaxin ELISA Kit
MBS732658-5x96StripWells 5x96-Strip-Wells
EUR 3020

Human Relaxin ELISA Kit
MBS732658-96StripWells 96-Strip-Wells
EUR 690

Human Relaxin-2 CLIA Kit
MBS8425885-INQUIRE INQUIRE Ask for price

Relaxin 2, mini (Human)
035-77 100 μg
EUR 405

Relaxin-2, human recombinant
7194-10 each
EUR 248.4

Relaxin-2, human recombinant
7194-50 each
EUR 810

Relaxin-2 Human Recombinant
rAP-4649 Inquiry Ask for price

RLN3 (GFP-tagged) - Human relaxin 3 (RLN3)
RG218745 10 µg Ask for price

Human Relaxin-2 ELISA Kit
E28L0578 96T
EUR 666.67

Human Relaxin-2 ELISA Kit
MBS761952-10x96StripWells 10x96-Strip-Wells
EUR 3900

Human Relaxin-2 ELISA Kit
MBS761952-48StripWells 48-Strip-Wells
EUR 340

Human Relaxin-2 ELISA Kit
MBS761952-5x96StripWells 5x96-Strip-Wells
EUR 2045

Human Relaxin-2 ELISA Kit
MBS761952-96StripWells 96-Strip-Wells
EUR 455

Human Relaxin-3/RLN3 ELISA Kit PicoKine®
EK1407 96 wells
EUR 750
Description: Human Relaxin 3 ELISA Kit PicoKine® (96 Tests). Quantitate Human RLN3 in cell culture supernatants, serum and plasma ( heparin). Sensitivity: 10pg/ml.

Human Relaxin (RLN) Protein
abx654931-100g 100 µg
EUR 4525

Human Relaxin (RLN) Protein
abx654931-10g 10 µg
EUR 1800

Human Relaxin (RLN) Protein
20-abx654931
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • 10 ug
  • 50 ug
  • 100 ug
  • 200 ug
  • 1 mg

Human Relaxin-3 receptor 2 (RXFP4) ELISA Kit
abx542655-96tests 96 tests
EUR 687.5

Human Relaxin-3 receptor 1 (RXFP3) ELISA Kit
AE20967HU-48T 48T
EUR 325
Description: Human (Homo sapiens)

Human Relaxin-3 receptor 1 (RXFP3) ELISA Kit
AE20967HU-48Tests 48 Tests
EUR 325
Description: Human (Homo sapiens)

Human Relaxin-3 receptor 1 (RXFP3) ELISA Kit
AE20967HU-96T 96T
EUR 610
Description: Human (Homo sapiens)

Human Relaxin-3 receptor 1 (RXFP3) ELISA Kit
AE20967HU-96Tests 96 Tests
EUR 610
Description: Human (Homo sapiens)

Human Relaxin-3 receptor 1 (RXFP3) ELISA Kit
EK7495 96Т
EUR 799

Human Relaxin-3 receptor 1 (RXFP3) ELISA Kit
KTE60775-48T 48T
EUR 339
Description: This Human Relaxin-3 receptor 1 (RXFP3) ELISA Kit employs a two-site sandwich ELISA to quantitate RXFP3.

Human Relaxin-3 receptor 1 (RXFP3) ELISA Kit
KTE60775-96T 96T
EUR 589
Description: This Human Relaxin-3 receptor 1 (RXFP3) ELISA Kit employs a two-site sandwich ELISA to quantitate RXFP3.

Human Relaxin-3 receptor 1 (RXFP3) ELISA Kit
KTE60775-96T5 96 T×5
EUR 2629
Description: This Human Relaxin-3 receptor 1 (RXFP3) ELISA Kit employs a two-site sandwich ELISA to quantitate RXFP3.
The patients with bigger skin lesion in CL and higher level of immunoglobulin or splenomegaly in VL, had the higher parasite load detected by qPCR. The parasite load was significantly reduced after treatment. In conclusion, the mkDNA-based qPCR assay that we developed in this study can be used not only for diagnosis of both cutaneous and visceral leishmaniasis with high sensitivity and specificity, but also for evaluating the severity and treatment efficacy of this disease, presenting a rapid and accurate tool for clinical surveillance, treatment monitoring and the end point determination of leishmaniasis.

Molecular Detection of the mcr Genes by Multiplex PCR

Background: The emergence and prevalence of plasmid-mediated colistin-resistant bacterial strains in recent years have raised great concerns in clinical medicine. It is urgently needed to develop a cheaper, faster, simpler, sensitive, and specific molecular detection method to identify and monitor the dissemination of the transferable resistant determinants.
Methods and results: Herein, eight pairs of primers were designed to set up a multiplex PCR method for the rapid and efficient determination of reported mcr genes. This assay can give results within 85 min (35 min for amplification and 50 min for electrophoresis). We validated the feasibility of this assay by testing the presence of mcr genes in 60 colistin-resistant isolates.
Conclusion: Our multiplex PCR technique exhibits remarkable advantages in the light of clear identification, efficiency of amplification, as well as the time consuming for detection, and thus could be useful for the surveillance and epidemiological research of plasmid-mediated colistin resistance, particularly for the under-resourced laboratories.

Use of the PCR in a Combined Methodological Approach for the Study of Human Fascioliasis in an Endemic Area

 

Purpose: Fascioliasis is a worldwide distributed trematodiasis considered a neglected disease. Diagnosis in humans has been traditionally based on parasitological and immunological techniques. Recently we reported the use of the PCR in stool samples for the individual diagnosis. The purpose of this study was to evaluate human fascioliasis by a combination of diagnostic methods in an area where the disease is highly endemic in animals.
Methods: We studied all the inhabitants (N = 240) of Tatón village, Argentina, by Fasciola hepatica rproCL1-ELISA. Among them, we continued the study with 13 cases that had at least two positive serological tests, who performed a questionnaire, physical examination, abdominal ultrasonography, and collection of blood and faeces. Blood/serum samples were used for Fh rproCL1-ELISA and liver function tests. Faeces were used for parasitological analysis and PCR of a repetitive fragment of Fasciola sp.
Results: Among the 13 patients, 9 presented symptoms of biliary colic. All patients repeated positive serology. F. hepatica eggs were not detected. PCR was positive in 11 cases.
Conclusion: This is the first report employing an approach based on the combination of methods for the evaluation of human fascioliasis in an endemic area, which includes molecular tools with a high value in detecting low infections.

Evaluation of a real-time PCR assay for diagnosis of schistosomiasis japonica in the domestic goat

 

Background: Schistosomiasis japonica is an infectious disease caused by Schistosoma japonicum that seriously endangers human health. Domestic animals have important roles in disease transmission and goats are considered a primary reservoir host and source of infection.
The prevalence and intensity of schistosomiasis infections have significantly decreased in China, and a more sensitive, specific detection method is urgently needed. The aim of this study was to develop a real-time PCR assay for accurate detection of S. japonicum infection in goats.
Methods: A real-time PCR method for detecting schistosomiasis japonica in goats was developed by amplification of a specific S. japonicum DNA fragment, and validated using a total of 94 negative and 159 positive plasma and serum samples collected in our previous study of S. japonicum infection.
Both plasma and serum samples were evaluated by real-time PCR and enzyme-linked immunosorbent assay (ELISA). In addition, 120 goat plasma samples from an S. japonicum-endemic area (Wangjiang) and 33 from a non-endemic region (Weihai) were collected and evaluated using our method.
Results: The sensitivity and specificity of the real-time PCR for detecting infected samples were 98.74% (157/159, 95% CI: 95.53-99.85%) and 100% (94/94, 95% CI: 96.15-100%), respectively. For the ELISA, sensitivity and specificity were 98.11% (156/159, 95% CI: 94.59-99.61%) and 90.43% (85/94, 95% CI: 82.60-95.53%), respectively. Further, we found positivity rates for S. japonicum infection in Wangjiang and Weihai of 8.33% (10/120, 95% CI: 4.07-14.79%) and 0% (0/33, 95% CI: 0-10.58%), respectively.
Conclusions: The results of this study indicate that our real-time PCR method exhibits higher sensitivity and specificity than ELISA and is a useful method for detection of S. japonicum infection in goats.

GPR160 Antibody
DF4899-200ul 200ul
EUR 210
Description: WB,IF/ICC,ELISA(peptide)

GPR160 Antibody
E047605 100μg/100μl
EUR 255
Description: Available in various conjugation types.

GPR160 Antibody
E11-140G 100μg
EUR 225
Description: Available in various conjugation types.

GPR160 Antibody
G140-100ul 100μl
EUR 217
Description: GPR160 Rabbit Polyclonal Antibody

GPR160 Antibody
G140-50ul 50μl
EUR 143.5
Description: GPR160 Rabbit Polyclonal Antibody

GPR160 Antibody
E19-4899 100μg/100μl
EUR 225
Description: Available in various conjugation types.

GPR160 Antibody
MBS5315557-01mL 0.1mL
EUR 470

GPR160 Antibody
MBS5315557-5x01mL 5x0.1mL
EUR 1955

GPR160 antibody
MBS535434-005mg 0.05mg
EUR 1125

GPR160 antibody
MBS535434-5x005mg 5x0.05mg
EUR 4905

GPR160 Antibody
MBS7119398-005mg 0.05mg
EUR 150

GPR160 Antibody
MBS7119398-01mg 0.1mg
EUR 190

GPR160 Antibody
MBS7119398-5x01mg 5x0.1mg
EUR 845

GPR160 Antibody
MBS8515601-01mg 0.1mg
EUR 305

GPR160 Antibody
MBS8515601-01mLAF405L 0.1mL(AF405L)
EUR 465

GPR160 Antibody
MBS8515601-01mLAF405S 0.1mL(AF405S)
EUR 465

GPR160 Antibody
MBS8515601-01mLAF610 0.1mL(AF610)
EUR 465

GPR160 Antibody
MBS8515601-01mLAF635 0.1mL(AF635)
EUR 465

GPR160 Antibody
MBS853183-01mg 0.1mg
EUR 305

GPR160 Antibody
MBS853183-01mLAF405L 0.1mL(AF405L)
EUR 465

GPR160 Antibody
MBS853183-01mLAF405S 0.1mL(AF405S)
EUR 465

GPR160 Antibody
MBS853183-01mLAF610 0.1mL(AF610)
EUR 465

GPR160 Antibody
MBS853183-01mLAF635 0.1mL(AF635)
EUR 465

GPR160 Antibody
MBS9232645-01mL 0.1mL
EUR 415

GPR160 Antibody
MBS9232645-5x01mL 5x0.1mL
EUR 1841

GPR160 Antibody
MBS9606351-01mL 0.1mL
EUR 260

GPR160 Antibody
MBS9606351-02mL 0.2mL
EUR 305

GPR160 Antibody
MBS9606351-5x02mL 5x0.2mL
EUR 1220

GPR160 Antibody
MBS9509528-005mL 0.05mL
EUR 285

GPR160 Antibody
MBS9509528-01mL 0.1mL
EUR 385

GPR160 Antibody
MBS9509528-5x01mL 5x0.1mL
EUR 1590

GPR160 Antibody
MBS9507949-005mL 0.05mL
EUR 285

GPR160 Antibody
MBS9507949-01mL 0.1mL
EUR 385

GPR160 Antibody
MBS9507949-5x01mL 5x0.1mL
EUR 1590

GPR160 Antibody
MBS9430587-01mL 0.1mL
EUR 305

GPR160 Antibody
MBS9430587-5x01mL 5x0.1mL
EUR 1230

GPR160 Polyclonal Antibody
ABP54526-003ml 0.03ml
EUR 189.6
Description: A polyclonal antibody for detection of GPR160 from Human. This GPR160 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human GPR160 at AA rangle: 270-350

GPR160 Polyclonal Antibody
ABP54526-01ml 0.1ml
EUR 346.8
Description: A polyclonal antibody for detection of GPR160 from Human. This GPR160 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human GPR160 at AA rangle: 270-350

GPR160 Polyclonal Antibody
ABP54526-02ml 0.2ml
EUR 496.8
Description: A polyclonal antibody for detection of GPR160 from Human. This GPR160 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human GPR160 at AA rangle: 270-350

GPR160 Conjugated Antibody
C47605 100ul
EUR 476.4

GPR160 Polyclonal Antibody
BT-AP03712-100ul 100ul Ask for price
Description: Orphan receptor.

GPR160 Polyclonal Antibody
BT-AP03712-20ul 20ul Ask for price
Description: Orphan receptor.

GPR160 Polyclonal Antibody
BT-AP03712-50ul 50ul Ask for price
Description: Orphan receptor.

GPR160 Polyclonal Antibody
E-AB-19949-120uL 120uL
EUR 240
Description: Unconjugated

GPR160 Polyclonal Antibody
E-AB-19949-200uL 200uL
EUR 399
Description: Unconjugated

GPR160 Polyclonal Antibody
E-AB-19949-20uL 20uL
EUR 73
Description: Unconjugated

GPR160 Polyclonal Antibody
E-AB-19949-60uL 60uL
EUR 143
Description: Unconjugated

GPR160 Polyclonal Antibody
JOT-AP03712-100ul 100ul
EUR 220
Description: Human

GPR160 Polyclonal Antibody
JOT-AP03712-50ul 50ul
EUR 144
Description: Human

GPR160 Polyclonal Antibody
E20-71990 100ug
EUR 225
Description: Available in various conjugation types.

GPR160 Polyclonal Antibody
E44H04883 100ul
EUR 255
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

GPR160 Polyclonal Antibody
MBS2561838-002mL 0.02mL
EUR 135

GPR160 Polyclonal Antibody
MBS2561838-006mL 0.06mL
EUR 190

GPR160 Polyclonal Antibody
MBS2561838-012mL 0.12mL
EUR 265

GPR160 Polyclonal Antibody
MBS2561838-02mL 0.2mL
EUR 415

GPR160 Polyclonal Antibody
MBS2561838-5x02mL 5x0.2mL
EUR 1835

GPR160 Polyclonal Antibody
MBS8522971-01mg 0.1mg
EUR 305

GPR160 Polyclonal Antibody
MBS8522971-01mLAF405L 0.1mL(AF405L)
EUR 465

GPR160 Polyclonal Antibody
MBS8522971-01mLAF405S 0.1mL(AF405S)
EUR 465

GPR160 Polyclonal Antibody
MBS8522971-01mLAF610 0.1mL(AF610)
EUR 465

GPR160 Polyclonal Antibody
MBS8522971-01mLAF635 0.1mL(AF635)
EUR 465

GPR160 Conjugated Antibody
MBS9455452-01mLAF350 0.1mL(AF350)
EUR 480

GPR160 Conjugated Antibody
MBS9455452-01mLAF405 0.1mL(AF405)
EUR 480

GPR160 Conjugated Antibody
MBS9455452-01mLAF488 0.1mL(AF488)
EUR 480

GPR160 Conjugated Antibody
MBS9455452-01mLAF555 0.1mL(AF555)
EUR 480

GPR160 Conjugated Antibody
MBS9455452-01mLBiotin 0.1mL(Biotin)
EUR 480

GPR160 Polyclonal Antibody
RD219949A-120uL 120μL
EUR 360
Description: Orphan receptor.

GPR160 Polyclonal Antibody
RD219949A-200uL 200μL
EUR 630
Description: Orphan receptor.

GPR160 Polyclonal Antibody
RD219949A-20uL 20μL
EUR 109.5
Description: Orphan receptor.

GPR160 Polyclonal Antibody
RD219949A-60uL 60μL
EUR 214.5
Description: Orphan receptor.

GPR160 Polyclonal Antibody
UB-GEN-13108 100 ul
EUR 200

GPR160 Antibody (C-term)
MBS9213578-008mL 0.08mL
EUR 210

GPR160 Antibody (C-term)
MBS9213578-04mL 0.4mL
EUR 430

GPR160 Antibody (C-term)
MBS9213578-5x04mL 5x0.4mL
EUR 1910

Polyclonal GPR160 Antibody (Internal)
APR16513G 0.05ml
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human GPR160 (Internal). This antibody is tested and proven to work in the following applications:

GPR160 Rabbit Polyclonal Antibody
E10G06629 100 μl
EUR 275
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

GPR160 Rabbit Polyclonal Antibody
ES5525-100ul 100ul
EUR 124
Description: WB, IHC, IF, ELISA

GPR160 Rabbit Polyclonal Antibody
ES5525-50ul 50ul
EUR 74
Description: WB, IHC, IF, ELISA

GPR160 Rabbit Polyclonal Antibody
E10G13515 100 μl
EUR 275
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

GPR160 Antibody - C-terminal region
MBS3217022-01mL 0.1mL
EUR 455

GPR160 Antibody - C-terminal region
MBS3217022-5x01mL 5x0.1mL
EUR 1995

GPR160 Antibody - N-terminal region
MBS3217023-01mL 0.1mL
EUR 455

GPR160 Antibody - N-terminal region
MBS3217023-5x01mL 5x0.1mL
EUR 1995

Polyclonal GPR160 Antibody (Extracellular Domain)
APR16512G 0.05mg
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human GPR160 (Extracellular Domain). This antibody is tested and proven to work in the following applications:

Anti- G Protein-Coupled Receptor GPR160/GPCR150 Human Antibody
GWB-84AB9F 0.05 mg Ask for price

GPR160 Antibody (C-term) Blocking Peptide
MBS9228244-INQUIRE INQUIRE Ask for price

Polyclonal GPR160 Antibody - C-terminal region
APR16514G 0.05mg
EUR 633.6
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human GPR160 - C-terminal region. This antibody is tested and proven to work in the following applications:

OAGA01201-100UL - GPR160 Antibody - middle region
OAGA01201-100UL 100ul
EUR 369

G Protein-Coupled Receptor 160 (GPR160) Antibody
abx324785-100g 100 µg
EUR 250

G Protein-Coupled Receptor 160 (GPR160) Antibody
20-abx324785
  • Ask for price
  • Ask for price
  • 50 ug
  • 100 ug
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