Genetic Distances of Rainbow Trout and Masu Salmon as Determined by PCR-Based Analysis

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Search for viral agents in cerebrospinal fluid in patients with multiple sclerosis using real-time PCR and metagenomics

 

Multiple sclerosis (MS) is a chronic, immune-mediated demyelinating disease of the central nervous system of unclear etiology, but there is some evidence that viral infections could be responsible for triggering autoimmune mechanisms against myelin. We searched for viral RNA and DNA in cerebrospinal fluid (CSF) of 34 MS patients and 13 controls using RT-PCR/PCR against common neurotropic viruses. In addition, shotgun DNA- and RNA-based metagenomics were done in 13 MS patients and 4 controls. Specific quantitative real-time RT-PCR/PCR testing revealed the presence of viral nucleic acid in seven (20.59%) MS patients and in one (7.69%) control patient. In MS patients the most frequently detected was human herpesvirus type 6 (HHV-6; 3 cases; 8.82%); followed by Epstein-Barr virus (EBV; 2 cases; 5.88%), varicella zoster virus (VZV; 1 case; 2.94%) and Enterovirus (EV; 1 case; 2.94%).
The single identified virus among controls was EBV (7.69%). DNA and RNA metagenomic assays did not identify any known eukaryotic viruses even though three of the analyzed samples were low-level positive by specific quantitative real-time PCR. In conclusion, we detected the presence of Herpesviridae and occasionally Enteroviridae in CSF from patients with MS but their prevalence was not significantly higher than among controls. Metagenomic analysis seems to be less sensitive than real-time RT-PCR/PCR and it did not detect any potential viral pathogens.

Pneumocystis jiroveci RT PCR kit

RTq-H440-100D 100T
EUR 860.4

Pneumocystis jiroveci RT PCR kit

RTq-H440-150D 150T
EUR 969.6

Pneumocystis jiroveci RT PCR kit

RTq-H440-50D 50T
EUR 717.6

Pneumocystis jiroveci One-Step PCR kit

Oneq-H440-100D 100T
EUR 1039.2

Pneumocystis jiroveci One-Step PCR kit

Oneq-H440-150D 150T
EUR 1177.2

Pneumocystis jiroveci One-Step PCR kit

Oneq-H440-50D 50T
EUR 861.6

Pneumocystis Stain Kit

PCS-1 1 kit(s)
EUR 187.2

Pneumocystis Stain Kit

PCS-2 100 Slides
EUR 124.8

Pneumocystis Stain Kit

K1434-125 each
EUR 510

Pneumocystis Stain Kit

K1434-30 each
EUR 379.2

Pneumocystis jiroveci

PCR-H440-PCRH44048D PCR-H440-48D
EUR 230

Pneumocystis jiroveci

PCR-H440-PCRH44096D PCR-H440-96D
EUR 312

Pneumocystis jiroveci

Oneq-H440-OneqH440100D Oneq-H440-100D
EUR 515

Pneumocystis jiroveci

Oneq-H440-OneqH440150D Oneq-H440-150D
EUR 594

Pneumocystis jiroveci

Oneq-H440-OneqH44050D Oneq-H440-50D
EUR 413

Pneumocystis jirovecii Control Slide

22251-1 1box
EUR 400

Fungi-Fluor® Pneumocystis Kit (Europe orders)

22363-E 1kit
EUR 736

Fungi-Fluor® Pneumocystis Kit (Europe orders)

22363E-1 1kit
EUR 736

Fungi-Fluor® Pneumocystis Kit (for U.S. & outside of Europe orders)

22363-1 - Ask for price

QPCR Kit DNA Pneumocysti

MOL8268 EACH
EUR 1045.79

QPCR Kit DNA Pneumocysti Mmix

MOL8270 EACH
EUR 1333.02

Monkeypox Virus Real Time PCR Kit

PDPS-AR064 1 unit Ask for price
Description: Creative Biogene Monkeypox Virus Real Time PCR Kit is used for the detection of monkeypox Virus in serum or lesion exudate samples by using real time PCR systems. Monkeypox virus (MPV) is a double-stranded DNA, zoonotic virus and a species of the genus Orthopoxvirus in the family Poxviridae. It is one of the human orthopoxviruses that includes variola (VARV), cowpox (CPX), and vaccinia (VACV) viruses. The kit contains a specific ready-to-use system for the detection of the monkeypox Virus. Fluorescence is emitted and measured by the real time systems' optical unit during the PCR.

Monkeypox Virus Real Time PCR Kit

YJC70115NW-25T 25 tests/kit Ask for price
Description: The Bioperfectus Monkeypox Virus Real Time PCR Kit is an in vitro diagnostic test, based on real-time PCR technology, for the detection of DNA from the Monkeypox virus. Specimens can be obtained from human serum, lesion exudate samples and scab. BSL-2 facilities with standard BSL-2 work practices may be used for the test of t he Monkeypox virus.

Monkeypox Virus Real Time PCR Kit

ZD-0076-01 25 tests/kit Ask for price
Description: Monkeypox virus is the virus that causes the disease monkeypox in both humans and animals. Monkeypox virus is an Orthopoxvirus, a genus of the family Poxviridae that contains other viral species that target mammals. The virus is mainly found in tropical rainforest regions of central and West Africa. The primary route of infection is thought to be contact with the infected animals or their bodily fluids. The genome is not segmented and contains a single molecule of linear double-stranded DNA, 185000 nucleotides long. The Monkeypox Virus real time PCR Kit contains a specific ready-to-use system for the detection of the Monkeypox Virusthrough polymerase chain reaction (PCR) in the real-time PCR system. The master contains reagents and enzymes for the specific amplification of the Monkeypox Virus DNA. Fluorescence is emitted and measured by the real time systems ́ optical unit during the PCR. The detection of amplified Monkeypox Virus DNA fragment is performed in fluorimeter channel 530nm with the fluorescent quencher BHQ1. DNA extraction buffer is available in the kit and serum or lesion exudate samples are used for the extraction of the DNA. In addition, the kit contains a system to identify possible PCR inhibition by measuring the 560nm fluorescence of the internal control (IC). An external positive control defined as 1×10^7 copies/ml is supplied which allow the determination of the gene load.

Monkeypox Virus Real Time PCR Kit

ZD-0076-02 25 tests/kit Ask for price
Description: Monkeypox virus is the virus that causes the disease monkeypox in both humans and animals. Monkeypox virus is an Orthopoxvirus, a genus of the family Poxviridae that contains other viral species that target mammals. The virus is mainly found in tropical rainforest regions of central and West Africa. The primary route of infection is thought to be contact with the infected animals or their bodily fluids.The genome is not segmented and contains a single molecule of linear double-stranded DNA, 185000 nucleotides long.The Monkeypox Virus real time PCR Kit contains a specific ready-to-use system for the detection of the Monkeypox Virusthrough polymerase chain reaction (PCR) in the real-time PCR system. The master contains reagents and enzymes for the specific amplification of theMonkeypox VirusDNA. Fluorescence is emitted and measured by the real time systems ́ optical unit during the PCR. The detection of amplified Monkeypox Virus DNA fragment is performed in fluorimeter channelFAM with the fluorescent quencher BHQ1. DNA extraction buffer is available in the kit and serum or lesion exudate samples are used for the extraction of the DNA. In addition, the kit contains a system to identify possible PCR inhibition by measuring the HEX/VIC/JOE fluorescence of the internal control (IC). An external positive control defined as 1×107copies/ml is supplied which allow the determination of the gene load.

Human Lung Cancer PCR Primer Library

HLUCPL-I 1 set
EUR 657.6

Human Schwann Cell PCR Primer Library

HSCH-I 1 set
EUR 657.6

PMA Real-Time PCR Bacterial Viability Kit - Legionella pneumophila (mip)

31053 1kit
EUR 513.6
Description: Minimum order quantity: 1 unit of 1kit

0.2ML CLEAR STRIP CAPS FOR REAL TIME PCR

PCR-2CP-RT-C 125/pk
EUR 267.6
Description: PCR Plates & Tubes; PCR Strip Tubes and Strip Caps - Axygen

AzuraQuant cDNA Synthesis Kit - 100 Reactions

AZ-1996 100 Reactions
EUR 446.4

AzuraQuant cDNA Synthesis Kit - 25 Reactions

AZ-1995 25 Reactions
EUR 183.6

Axygen Polyester ultra clear sealing film for real time PCR

AXY2152 PK100
EUR 382.8

Eztime Real - time PCRPremix 2x,Taqman

FYT105-100P 100 Preps 1.25 ml Ask for price

Eco 48 Real Time PCR system including software and loading dock

MOL6348 EACH
EUR 18466.8

Eztime Real - time PCRPremix 2x,SYBR Green

FYT103-100P 100 Preps 1.25 ml Ask for price

Eztime Real - time PCRPremix 2x,SYBR Green

FYT103-400P 400 Preps 1.25 ml x 4 Ask for price

Eztime Real - time PCRPremix 2x,Taqman, ROX

FYT105-400P 400 Preps 1.25 ml x 4 Ask for price

Eztime Real - time PCRPremix 2x,Taqman, ROX

FYT106-100P 100 Preps 1.25 ml Ask for price

Eztime Real - time PCRPremix 2x,SYBRGreen , ROX

FYT104-100P 100 Preps 1.25 ml Ask for price

Eztime Real - time PCRPremix 2x,SYBRGreen , ROX

FYT104-400P 400 Preps 1.25 ml x 4 Ask for price

Novel Coronavirus COVID-19 (2019-nCoV) Real Time RT-PCR Kit

RR-0478-02 25 tests/kit
EUR 1189.2
Description: Novel Coronavirus (2019-nCoV) Real Time RT-PCR Kit is used for the qualitative detection of a novel coronavirus, which was identified in 2019 at Wuhan City, Hubei Province, China, in upper respiratory tract specimens (nasopharyngeal extracts, deep cough sputum, etc.) and lower respiratory tract specimens (alveoli irrigation fluid, etc.) by real time PCR systems.

Green Dye One Step qRT-PCR Master Mix For Quantitative Real Time PCR With ROX Dye

MB1004-100Reactions 100 Reactions
EUR 392.4

Azura PureView 1 Kb DNA Ladder - 100 Lanes

AZ-1101 100 Lanes
EUR 140.4

Azura PureView 1 Kb DNA Ladder - 500 Lanes

AZ-1105 500 Lanes
EUR 277.2

Azura PureView 1 Kb DNA Ladder - 1000 Lanes

AZ-1105-2 1000 Lanes
EUR 326.4

Azura PureView 250bp DNA Ladder - 100 Lanes

AZ-1121 100 Lanes
EUR 140.4

Azura PureView 250bp DNA Ladder - 500 Lanes

AZ-1125 500 Lanes
EUR 277.2

Azura PureView 250bp DNA Ladder - 1000 Lanes

AZ-1125-2 1000 Lanes
EUR 326.4

Azura PureView 100bp DNA Ladder - 100 Lanes

AZ-1131 100 Lanes
EUR 140.4

Azura PureView 100bp DNA Ladder - 500 Lanes

AZ-1135 500 Lanes
EUR 277.2

Azura PureView 100bp DNA Ladder - 1000 Lanes

AZ-1135-2 1000 Lanes
EUR 326.4

Azura PureView 50bp DNA Ladder - 100 Lanes

AZ-1151 100 Lanes
EUR 140.4

Azura PureView 50bp DNA Ladder - 500 Lanes

AZ-1155 500 Lanes
EUR 277.2

Azura PureView 50bp DNA Ladder - 1000 Lanes

AZ-1155-2 1000 Lanes
EUR 326.4

AzuraView GreenFast qPCR Blue Mix Lo Rox - 200 Rxn

AZ-2301 200 Rxn
EUR 214.8

AzuraView GreenFast qPCR Blue Mix Lo Rox - 500 Rxn

AZ-2305 500 Rxn
EUR 384

AzuraView GreenFast qPCR Blue Mix Lo Rox - 2000 Rxn

AZ-2320 2000 Rxn
EUR 1195.2

AzuraView GreenFast qPCR Blue Mix Lo Rox - 5000 Rxn

AZ-2325 5000 Rxn
EUR 2817.6

AzuraView GreenFast qPCR Blue Mix Hi Rox - 200 Rxn

AZ-2401 200 Rxn
EUR 214.8

AzuraView GreenFast qPCR Blue Mix Hi Rox - 500 Rxn

AZ-2405 500 Rxn
EUR 384

AzuraView GreenFast qPCR Blue Mix Hi Rox - 2000 Rxn

AZ-2420 2000 Rxn
EUR 1195.2

AzuraView GreenFast qPCR Blue Mix Hi Rox - 5000 Rxn

AZ-2421 5000 Rxn
EUR 2817.6

Mouse Gdnf Signaling Primer Library

MGDNF-I 1 set
EUR 657.6

Mouse Glucose Metabolism Primer Library

MGLM-I 1 set
EUR 657.6

Mouse Hippo Signaling Primer Library

MHPO-I 1 set
EUR 657.6

Mouse Hypoxia Signaling Primer Library

MHYP-I 1 set
EUR 657.6

Mouse Notch Signaling Primer Library

MNOT-I 1 set
EUR 657.6

Mouse Neurotransmitter Signaling Primer Library

MNTS-I 1 set
EUR 657.6

Mouse Integrin Signaling Primer Library

MINT-I 1 set
EUR 891.6

Mouse Oxidative Stress Primer Library

MOSL-I 1 set
EUR 657.6

Mouse Oncogene Primer Library

MONC-I 1 set
EUR 657.6

Mouse Apoptosis Primer Library

MPA-I 1 set
EUR 540

Mouse Ras Signaling Primer Library

MRAS-I 1 set
EUR 657.6

Mouse T-Cell Receptor Signaling Primer Library

MTCR-I 1 set
EUR 657.6

Mouse TGF Beta Signaling Primer Library

MTGFB-I 1 set
EUR 657.6

Mouse Tumor Invasion/Metastasis Primer Library

MTIM-I 1 set
EUR 540

Mouse Tumor Immunity Primer Library

MTIMU-I 1 set
EUR 657.6

Mouse p53 Signaling Primer Library

MTPS-I 1 set
EUR 540

Mouse WNT Signaling Primer Library

MWNT-I 1 set
EUR 657.6

Mouse Toll-like Receptor Signaling Primer Library

newitem 1 set
EUR 657.6

Mouse PI3K-AKT Signaling Primer Library

MAKT-I 1 set
EUR 657.6

Mouse Angiogenesis Primer Library

MAPL-I 1 set
EUR 540

Mouse Adherin - Tight Junction Primer Library

MATJ-I 1 set
EUR 657.6

Mouse Autophagy Primer Library

MATPL-I 1 set
EUR 540

Mouse Bdnf Signaling Primer Library

MBDNF-I 1 set
EUR 657.6

Mouse BMP Signaling Primer Library

MBMP-I 1 set
EUR 657.6

Mouse Cytokine Primer Library I

MCA-I 1 set
EUR 540

Mouse Cytokine Primer Library II

MCA-II 1 set
EUR 540

Mouse Cell Cycle Primer Library

MCC-I 1 set
EUR 540

Mouse Cytokine and Chemokine Receptor Primer Library

MCCR-I 1 set
EUR 774

Mouse CREB Signaling Primer Library

MCREB-I 1 set
EUR 657.6

Mouse DNA Damage Signaling Primer Library

MDDS-I 1 set
EUR 657.6

Mouse DNA Repair Primer Library I

MDRL-I 1 set
EUR 774

Mouse DNA Repair Primer Library II

MDRL-II 1 set
EUR 774

Mouse Adipogenesis Primer Library

MADG-I 1 set
EUR 657.6

Mouse EGFR Signaling Primer Library

MEGFR-I 1 set
EUR 657.6

Mouse Epithelial-Mesenchymal Transition Primer Library

MEMT-I 1 set
EUR 657.6

Mouse Estrogen Receptor Signaling Library

MESR-I 1 set
EUR 774

Mouse Fatty Liver Signaling Primer Library

MFTL-I 1 set
EUR 657.6

Mouse Jak/Stat Signaling Primer Library

MJAK-I 1 set
EUR 774

Mouse MAP Kinase Signaling Primer Library

MMAP-I 1 set
EUR 657.6

Mouse Myeloid Derived Suppressor Cell Primer Library

MMDSC-I 1 set
EUR 657.6

Mouse NFKappaB Primer Library

MNFKB-I 1 set
EUR 540

Human DNA Repair Primer Library I

HDRL-I 1 set
EUR 657.6

Human DNA Repair Primer Library II

HDRL-II 1 set
EUR 657.6

Human Drug Transporter I Primer Library

HDTP-I 1 set
EUR 657.6

Human Drug Detoxication I

HDTX-I 1 set
EUR 657.6

Human Drug Detoxication II

HDTX-II 1 set
EUR 774

Human EGFR Signaling Primer Library

HEGFR-I 1 set
EUR 657.6

Human Epithelial Mesenchymal Transition Primer Library

HEMT-I 1 set
EUR 657.6

Human Endothelial Cell Primer Library

HEND-I 1 set
EUR 657.6

Human ERBB Signaling Primer Library

HERBB-I 1 set
EUR 657.6

Human Estrogen Signaling Primer Library

HESR-I 1 set
EUR 774

Human Focal Adhesion Signaling Primer Library

HFCA-I 1 set
EUR 657.6

Human FGF Receptor Signaling Primer Library

HFGF-I 1 set
EUR 774

Human Fatty Acid and Lipid Metabolism Primer Library

HFLM-I 1 set
EUR 657.6

Human Gastric Cancer Primer Library

HGCPL-I 1 set
EUR 657.6

Human GDNF Signaling Primer Library

HGDNF-I 1 set
EUR 657.6

Human Glucose Metabolism Primer Library

HGLM-I 1 set
EUR 540

Human G-Protein Coupled Receptor Signaling Primer Library

HGPCR-I 1 set
EUR 657.6

Human Hedgehog Signaling Primer Library

HHOG-I 1 set
EUR 774

Human Homeobox Gene Primer Library

HHOX-I 1 set
EUR 774

Human Hippo Signaling Primer Pathway

HHPO-I 1 set
EUR 657.6

Human Heat Shock Protein Primer Library

HHSP-I 1 set
EUR 891.6

Human Hypoxia Signaling Primer Library

HHYP-I 1 set
EUR 657.6

Human Interferon Type I Signaling Primer Library

HIFN-I 1 set
EUR 657.6

Genetic Distances of Rainbow Trout and Masu Salmon as Determined by PCR-Based Analysis

 

This study used a PCR-based genetic analysis platform to create a hierarchical polar dendrogram of Euclidean genetic distances for two salmonid species, Oncorhynchus mykiss (rainbow trout, RT) and Oncorhynchus masou (masu salmon, MS). The species were distantly related to other fish species based on PCR results from using the designed oligonucleotide primer series. Five oligonucleotide primers were used to generate 330 and 234 scorable fragments in the RT and MS populations, respectively. The DNA fragments ranged in size from approximately 50 bp to more than 2,000 bp.
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The bandsharing (BS) results showed that the RT population had a higher average BS value (0.852) than that for the MS population (0.704). The genetic distance between individuals supported the presence of adjacent affiliation in cluster I (RT 01-RT 11). The observation of a significant genetic distance between the two Oncorhynchus species verifies that this PCR-based technique can be a useful approach for individual- and population-based biological DNA investigations. The results of this type of investigation can be useful for species safekeeping and the maintenance of salmonid populations in the mountain streams of Korea.

Multiplex real-time PCR using double-strand primers and probes for the detection of nucleic acids

 

Multiplex PCR encounters difficulties in primer designing with all the primer pairs working at the same annealing temperature. In this study, we have developed a double-strand primer-mediated multiple strand displacement reaction for the detection of SARS-COV-2 ORF, N and E genes (as examples). The double primer is composed of a 5′-modified fluorophore strand, which does not impact polymerase extension and a 3′-modified quencher strand, which cannot impact elongation. At the annealing temperature, the fluorophore strand combined with the template, extended and resulted in fluorescence signal release.
Results showed that the double-strand primer relatively exhibits a wide annealing temperature range and good compatibility between three pairs of primers and probes. These merits allow the simple and multiplex real-time fluorescence quantification of nucleic acids. The detection limit was 400 copies/mL, and the detection time was approximately 2 h. In addition to its extreme specificity and simplicity, this method has a wide range of applications such as multiple PCR and SNP detection.

Neonatal Acute Lymphoblastic Leukemia with t(9;11) Translocation Presenting as Blueberry Muffin Baby: Successful Treatment by ALL-BFM Induction Therapy, Allogeneic Stem Cell Transplantation from an Unrelated Donor, and PCR-MRD-Guided Post-Transplant Follow-Up

 

BACKGROUND Neonatal acute leukemia is a rare condition. Little is known about its incidence and outcomes, and treatment options have not been standardized. CASE REPORT A 3-day old, apparently healthy male newborn was referred to the pediatric intensive care unit with multiple violaceous macules and a few papules on his face and upper trunk. After initial spontaneous regression, the lesions reappeared. Skin biopsy and bone marrow aspirate revealed a diagnosis of acute lymphoblastic leukemia (ALL). ALL induction therapy was initiated on day 24, resulting in morphological remission at the end of induction therapy. ALL chemotherapy was guided by sequential PCR-based monitoring of minimal residual disease (MRD).
The patient received a transplant from an unrelated HLA high-resolution matched (10/10 loci) permissive donor. He was followed-up after transplant conducted by sequential PCR-based measurements of MRD in bone marrow. CONCLUSIONS Neonatal leukemia often presents as congenital skin lesions known as blueberry muffin rash. ALL induction therapy was started at the end of the neonatal period. Treatment was well-tolerated and effective. Early donor search and PCR-MRD guided treatment surveillance can help to achieve and maintain molecular remission.

Sex determination from the pulp tissue of deciduous teeth exposed to natural soil and wet clay – A PCR study

 

Context: Dental tissue remains are the toughest, and chemically, the most stable tissue in the body. Its high resilience in the events of fire and bacterial decomposition makes them vital for DNA analysis by PCR method.
Aims: Determination of sex of children through molecular analysis of pulp tissue of exfoliated deciduous teeth stored in different media and analyzed after a different time period.
Settings and design: Sixty samples of deciduous teeth were divided into three groups. Group IA and Group IIA were stored in natural soil and wet clay for 1 month, respectively. Group IB and Group IIB were stored in natural soil and wet clay for 6 months, respectively. Group III was analyzed immediately after extraction.
Methods and material: Sex determination was carried out in five steps: Pulp tissue removal, DNA isolation, DNA quantification, PCR amplification, Sex determination. X and Y specific chromosomes from each sample were amplified and compared.
Statistical analysis used: Kruskal-Wallis test, Dunn’s test, and Wilcoxon signed rank test.
Results: Group III revealed the highest amount of DNA quantified. Amount of DNA quantified after 6 months of storage in natural soil and wet clay decreased in both the groups with the samples stored in wet clay showing a maximum decrease. Results of the PCR analysis also showed 100% accuracy rate in the samples of Group III.
Conclusions: Sex determination from pulp tissue depends a lot on the quality and quantity of DNA extracted. Sex could be effectively determined among the samples evaluated immediately after extraction. This ability decreases as the storage condition changes and the time period increases. Samples stored in wet clay were found to show the least sex identification ability than dry soil.

qPCR ProbesMaster highROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2x conc. master mix** shipped ice packs - must be shipped via overnight service

M-PCR-362S 2 x 1,25 ml
EUR 205
Description: qPCR ProbesMaster highROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2x conc. master mix** shipped ice packs - must be shipped via overnight service

qPCR ProbesMaster lowROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service

M-PCR-361L 10 x 1,25 ml
EUR 818
Description: qPCR ProbesMaster lowROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service

qPCR ProbesMaster lowROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service

M-PCR-361S 2 x 1,25 ml
EUR 205
Description: qPCR ProbesMaster lowROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service

qPCR ProbesMaster UNG lowROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service

M-PCR-364L 10 x 1,25 ml
EUR 982
Description: qPCR ProbesMaster UNG lowROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service

qPCR ProbesMaster UNG lowROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service

M-PCR-364S 2 x 1,25 ml
EUR 246
Description: qPCR ProbesMaster UNG lowROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service

qPCR ProbesMaster UNG highROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service

M-PCR-365L 10 x 1,25 ml
EUR 982
Description: qPCR ProbesMaster UNG highROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service

qPCR ProbesMaster UNG highROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service

M-PCR-365S 2 x 1,25 ml
EUR 246
Description: qPCR ProbesMaster UNG highROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service

RT-PCR quantitative Covid-19 assay CE-IVD

CLO-RT-25 48 reactions Ask for price
Description: RT-PCR quantitative Covid-19 assay CE-IVD

Tiamulin ELISA Kit, Quantitative

DEIA036 96T
EUR 1414.8
Description: This kit is intended for quantitative analysis of tiamulin residue in animal tissue (pork and chicken, etc).

Human RBP4 Quantitative ELISA Kit

LF-EK70030 1×96T
EUR 722.4

Mouse NoroVirus ELISA Kit(Quantitative )

SL0661Mo -
EUR 528

Rat C3 ELISA Kit, 96 tests, Quantitative

6230 1 kit
EUR 854.4

Human Mumps IgG ELISA Kit (quantitative)

SL3197Hu 96 Tests
EUR 468

Cat IgG ELISA Kit, 96 tests, Quantitative

300-100-CGG 1 kit
EUR 854.4

Cat IgM ELISA kit 96 tests, Quantitative

300-170-CGM 1 kit
EUR 854.4

Cat IgA ELISA Kit, 96 tests, Quantitative

300-180-CGA 1 kit
EUR 854.4

Cat IgE ELISA Kit, 96 tests, Quantitative

300-190-CGE 1 kit
EUR 854.4

Dog IgG ELISA Kit, 96 tests, Quantitative

400-100-DGG 1 Kit
EUR 781.2

Dog IgM ELISA Kit, 96 tests, Quantitative

400-170-DGM 1 Kit
EUR 781.2

Dog IgA ELISA Kit, 96 tests, Quantitative

400-180-DGA 1 Kit
EUR 854.4

Dog IgE ELISA Kit, 96 tests, Quantitative

400-190-DGE 1 Kit
EUR 854.4

Rat IgG ELISA Kit, 96 tests, Quantitative

6420 1 kit
EUR 781.2

Rat IgE ELISA Kit, 96 tests, Quantitative

6470 1 kit
EUR 927.6

Rat IgM ELISA Kit, 96 tests, Quantitative

6480 1 Kit
EUR 854.4

Rat IgA ELISA kit 96 tests, Quantitative

6410-10 1 kit
EUR 607.2

Pig IgM ELISA Kit, 96 tests, Quantitative

9080 1 kit
EUR 854.4

Lactate ELISA Kit, 96 tests, Quantitative

10795 1 Kit
EUR 927.6

Rat VEGF ELISA Kit, 96 tests, quantitative

200-840-VER 1 kit
EUR 927.6

Goat IgM ELISA Kit, 96 tests, Quantitative

7530 1 kit
EUR 927.6

Goat IgA ELISA Kit, 96 tests, Quantitative

7540 1 kit
EUR 1000.8

Goat IgE ELISA Kit, 96 tests, Quantitative

7550 1 kit
EUR 1000.8

Rat IgG1 ELISA Kit, 96 tests, Quantitative

6430 1 Kit
EUR 854.4

Oxytocin ELISA Kit, 96 tests, Quantitative

10790 1 Kit
EUR 927.6

Human IgG ELISA Kit, 96 tests, Quantitative

1750 1 Kit
EUR 781.2

Human IgM ELISA Kit, 96 tests, Quantitative

1760 1 Kit
EUR 781.2

Human IgA ELISA Kit, 96 tests, Quantitative

1780 1 kit
EUR 781.2

Sheep IgM ELISA Kit, 96 tests, Quantitative

7630 1 kit
EUR 854.4

Sheep IgA ELISA Kit, 96 tests, Quantitative

7640 1 kit
EUR 1000.8

Sheep IgE ELISA Kit, 96 tests, Quantitative

7650 1 kit
EUR 1000.8

Horse IgG ELISA Kit, 96 tests, Quantitative

7730 1 kit
EUR 854.4

Horse IgM ELISA Kit, 96 tests, Quantitative

7740 1 kit
EUR 854.4

Horse IgE ELISA Kit, 96 tests, Quantitative

7750 1 kit
EUR 927.6

Mouse IgA ELISA Kit, 96 tests, Quantitative

6310 1 kit
EUR 781.2

Mouse IgG ELISA Kit, 96 tests, Quantitative

6320 1 kit
EUR 781.2

Mouse IgE ELISA Kit, 96 tests, Quantitative

6370 1 Kit
EUR 781.2

Mouse IgM ELISA Kit, 96 tests, Quantitative

6380 1 Kit
EUR 781.2

Rat IgG2a ELISA Kit, 96 tests, Quantitative

6440 1 Kit
EUR 578.4

Rat IgG2b ELISA Kit, 96 tests, Quantitative

6450 1 Kit
EUR 634.8

Duck Pasteurellosis ELISA Kit(Quantitative)

SL0014Du -
EUR 528

G. Pig IgM ELISA Kit, 96 tests, Quantitative

7430 1 kit
EUR 781.2

Mouse VEGF ELISA Kit, 96 tests, Quantitative

100-230-VEM 1 Kit
EUR 854.4

Vitamin A ELISA Kit, 96 tests, Quantitative

10760 1 Kit
EUR 927.6

Vitamin B1 ELISA Kit, 96 tests, Quantitative

10765 1 Kit
EUR 927.6

Vitamin C ELISA Kit, 96 tests, Quantitative

10770 1 Kit
EUR 927.6

Vitamin E ELISA Kit, 96 tests, Quantitative

10775 1 Kit
EUR 927.6

Turkey IgG ELISA Kit, 96 tests, Quantitative

8020 1 kit
EUR 781.2

Horse Fab2 ELISA Kit, 96 tests, Quantitative

7710-Fab 1 kit
EUR 854.4

Mouse IgG1 ELISA Kit, 96 tests, Quantitative

6330 1 kit
EUR 781.2

Mouse IgG3 ELISA Kit, 96 tests, Quantitative

6360 1 kit
EUR 781.2

Rabbit IgA ELISA Kit, 96 tests, Quantitative

6510 1 kit
EUR 927.6

Rabbit IgM ELISA Kit, 96 tests, Quantitative

6580 1 kit
EUR 708

Bovine IgA ELISA Kit, 96 tests, Quantitative

8075 1 Kit
EUR 854.4

Bovine IgM ELISA Kit, 96 tests, Quantitative

8080 1 Kit
EUR 854.4

Mouse CCL2 ELISA Kit, 96 tests, Quantitative

MCC-0L2-100 1 kit
EUR 578.4

Mouse CCL3 ELISA Kit, 96 tests, Quantitative

MCC-0L3-100 1 kit
EUR 607.2

Human CCL2 ELISA Kit, 96 tests, Quantitative

HCC-0L2-105 1 kit
EUR 578.4

Human CCL3 ELISA Kit, 96 tests, Quantitative

HCC-0L3-110 1 kit
EUR 578.4

Human CCL4 ELISA Kit, 96 tests, Quantitative

HCC-0L4-115 1 kit
EUR 578.4

Human CD93 ELISA Kit, 96 tests, Quantitative

HCD-093-120 1 kit
EUR 578.4

Mouse parvovirus (PV) ELISA Kit(Quantitative )

SL0660Mo -
EUR 528

Protein-A ELISA Kit, 480 tests, Quantitative

800-110-PRA-5 1 pk
EUR 3714

Benedicts Solution Quantitative

RRSP900-D 500ml
EUR 6.36

Benedicts Solution Quantitative

RRSP900-E 1L
EUR 10.59

Benedicts Solution Quantitative

RRSP900-F 2.5L
EUR 21.96

Rat IL-13 ELISA Kit, 96 tests, Quantitative

RIL-013-100 1 kit
EUR 578.4

Vasopressin ELISA Kit, 96 tests, Quantitative

10800 1 Kit
EUR 927.6

Pig Insulin ELISA Kit, 96 tests, Quantitative

10420 1 Kit
EUR 927.6

Vitamin B12 ELISA kit, 96 tests, Quantitative

0340-B12 1 Kit
EUR 927.6

D-Lactate ELISA Kit, 96 tests, Quantitative

10845 1 Kit
EUR 927.6

Protein-A ELISA Kit, 96 tests, Quantitative

800-110-PRA 1 kit
EUR 927.6

Mouse IgG2a ELISA Kit, 96 tests, Quantitative

6340 1 kit
EUR 781.2

Mouse IgG2b ELISA Kit, 96 tests, Quantitative

6350 1 kit
EUR 781.2

Dog Albumin ELISA Kit, 96 tests, Quantitative

7000-50 1 kit
EUR 781.2

Rat Albumin ELISA Kit, 96 tests, Quantitative

6400 1 kit
EUR 781.2

Mouse CXCL3 ELISA Kit, 96 tests, Quantitative

MCXC-003-100 1 kit
EUR 578.4

Human CXCL3 ELISA Kit, 96 tests, Quantitative

HCXC-003-100 1 kit
EUR 578.4

Human CXCL9 ELISA Kit, 96 tests, Quantitative

HCXC-090-100 1 kit
EUR 578.4

Mouse IL-2 ELISA Kit, 96 tests, Quantitative

MIL-002-105 1 kit
EUR 578.4

Mouse IL-4 ELISA Kit, 96 tests, Quantitative

MIL-004-110 1 kit
EUR 578.4

Mouse IL-5 ELISA Kit, 96 tests, Quantitative

MIL-005-115 1 kit
EUR 578.4

Mouse IL-9 ELISA Kit, 96 tests, Quantitative

MIL-009-120 1 kit
EUR 578.4

Human IL-4 ELISA Kit, 96 tests, Quantitative

HIL-004-105 1 kit
EUR 578.4

Human IL-5 ELISA Kit, 96 tests, Quantitative

HIL-005-110 1 kit
EUR 578.4

Human IL-9 ELISA Kit, 96 tests, Quantitative

HIL-009-115 1 kit
EUR 607.2

Human IL-8 ELISA Kit, 96 tests, Quantitative

HIL-080-170 1 kit
EUR 578.4

Rat IL-17A ELISA Kit, 96 tests, Quantitative

RIL-17A-100 1 kit
EUR 607.2

Homocysteine ELISA kit, 96 tests, Quantitative

0370-HCY 1 kit
EUR 927.6

Prostacyclin ELISA Kit, 96 tests, Quantitative

10785 1 Kit
EUR 927.6

Mouse Leptin ELISA Kit, 96 tests, Quantitative

100-130-LEM 1 Kit
EUR 854.4

Goat Insulin ELISA Kit, 96 tests, Quantitative

10730 1 Kit
EUR 927.6

Rat Glucagon ELISA Kit, 96 tests, Quantitative

9485 1 Kit
EUR 927.6

Human CXCL10 ELISA Kit, 96 tests, Quantitative

HCXC-010-100 1 kit
EUR 708

Human CXCL16 ELISA Kit, 96 tests, Quantitative

HCXC-016-100 1 kit
EUR 578.4

Bovine IL-4 ELISA Kit, 96 tests, Quantitative

BIL-004-100 1 kit
EUR 634.8

Mouse IL-10 ELISA Kit, 96 tests, Quantitative

MIL-010-125 1 kit
EUR 578.4

Mouse IL-13 ELISA Kit, 96 tests, Quantitative

MIL-013-135 1 kit
EUR 578.4

Mouse IL-21 ELISA Kit, 96 tests, Quantitative

MIL-021-155 1 kit
EUR 607.2

Mouse IL-22 ELISA Kit, 96 tests, Quantitative

MIL-022-160 1 kit
EUR 607.2

Mouse IL-23 ELISA Kit, 96 tests, Quantitative

MIL-023-165 1 kit
EUR 607.2

Mouse IL-25 ELISA Kit, 96 tests, Quantitative

MIL-025-170 1 kit
EUR 607.2

Mouse IL-27 ELISA Kit, 96 tests, Quantitative

MIL-027-175 1 kit
EUR 607.2

Mouse IL-33 ELISA Kit, 96 tests, Quantitative

MIL-033-180 1 kit
EUR 607.2

Human IL-10 ELISA Kit, 96 tests, Quantitative

HIL-010-120 1 kit
EUR 578.4

Human IL-13 ELISA Kit, 96 tests, Quantitative

HIL-013-130 1 kit
EUR 607.2

Human IL-15 ELISA Kit, 96 tests, Quantitative

HIL-015-135 1 kit
EUR 607.2

Human IL-21 ELISA Kit, 96 tests, Quantitative

HIL-021-145 1 kit
EUR 578.4

Human IL-22 ELISA Kit, 96 tests, Quantitative

HIL-022-150 1 kit
EUR 607.2

Human IL-23 ELISA Kit, 96 tests, Quantitative

HIL-023-155 1 kit
EUR 607.2

Human IL-27 ELISA Kit, 96 tests, Quantitative

HIL-027-160 1 kit
EUR 607.2

Human IL-29 ELISA Kit, 96 tests, Quantitative

HIL-029-165 1 kit
EUR 578.4

Human Estrone ELISA Kit, 96 tests, Quantitative

1925 1 kit
EUR 708

Rat Clusterin ELISA kit 96 tests, Quantitative

610-110-RCL 1 Kit
EUR 927.6

Rat Hemopexin ELISA kit 96 tests, Quantitative

610-710-HPX 1 Kit
EUR 927.6

Mouse Albumin ELISA Kit, 96 tests, Quantitative

6300 1 kit
EUR 854.4

Pig Myoglobin ELISA kit 96 tests, Quantitative

600-640-PMY 1 Kit
EUR 1000.8

Rat Myoglobin ELISA kit 96 tests, Quantitative

600-660-RMY 1 kit
EUR 1000.8

Human Glucose ELISA Kit, 96 tests, Quantitative

9575 1 Kit
EUR 927.6

Sheep IgG-Fc ELISA Kit, 96 tests, Quantitative

7615-Fc 1 kit
EUR 854.4

Horse IgG-Fc ELISA Kit, 96 tests, Quantitative

7715-Fc 1 kit
EUR 854.4

Mouse GM-CSF ELISA Kit, 96 tests, Quantitative

MGM-CSF-100 1 kit
EUR 578.4

Mouse IL-17F ELISA Kit, 96 tests, Quantitative

MIL-017-140 1 kit
EUR 607.2

Mouse IL-17A ELISA Kit, 96 tests, Quantitative

MIL-17A-185 1 kit
EUR 578.4

Human GM-CSF ELISA Kit, 96 tests, Quantitative

HGM-CSF-100 1 kit
EUR 578.4

Human IL-17A ELISA Kit, 96 tests, Quantitative

HIL-17A-175 1 kit
EUR 578.4

Human IL-17F ELISA Kit, 96 tests, Quantitative

HIL-17F-185 1 kit
EUR 607.2

Corticosterone ELISA Kit, 96 tests, Quantitative

CRS-100 1 kit
EUR 854.4

Alpha-Fetoprotein, AFP ELISA Kit (Quantitative)

DEIA080 96T
EUR 602.4
Description: This AFP ELISA kit is an enzyme linked immunosorbent assay (ELISA) for in vitro quantitative determination of α-fetoprotein (AFP) concentrations in the range of 2-400ng/mL in human serum or plasma samples.

Quantitative Pipettes Bulb Fillers

PF330 1 UNIT
EUR 66.26

Goat Prolactin ELISA Kit, 96 tests, Quantitative

10735 1 Kit
EUR 927.6

Human Cortisol ELISA Kit, 96 tests, Quantitative

1850 1 kit
EUR 634.8

Monkey Albumin ELISA Kit, 96 tests, Quantitative

7000-10 1 Kit
EUR 927.6

Rat Thiostatin ELISA kit 96 tests, Quantitative

600-730-RTS 1 Kit
EUR 927.6

Human Thrombin ELISA Kit, 96 tests, Quantitative

9625 1 Kit
EUR 927.6

Mouse Glucagon ELISA Kit, 96 tests, Quantitative

9335 1 Kit
EUR 1074

Rat IL-1 beta ELISA Kit, 96 tests, Quantitative

RIL-01B-100 1 kit
EUR 578.4

Lipoxin A4 (LXA4) ELISA Kit, 96 tests, Quantitative

10885 1 Kit
EUR 927.6

Sheep/Ovine Fab ELISA Kit, 96 tests, Quantitative

7610-Fab 1 kit
EUR 854.4

Mouse IL-17AF ELISA Kit, 96 tests, Quantitative

MIL-17AF-190 1 kit
EUR 578.4

Human IL-17AF ELISA Kit, 96 tests, Quantitative

HIL-17AF-180 1 kit
EUR 607.2

Protein-G (HCPs) ELISA Kit, 480 tests, Quantitative

800-120-PRG-5 1 pk
EUR 3714

Mouse Myostatin ELISA Kit, 96 tests, Quantitative

100-220-MYM 1 Kit
EUR 1000.8

Hyaluronic Acid ELISA Kit, 96 tests, Quantitative

10850 1 Kit
EUR 927.6

Chicken Insulin ELISA Kit, 96 tests, Quantitative

10655 1 Kit
EUR 927.6

Human Estradiol ELISA Kit, 96 tests, Quantitative

1920 1 kit
EUR 708

Total Bilirubin Assay Kit, Quantitative, 96 tests

BBN-100 1 kit
EUR 854.4

Dog Transferrin ELISA Kit, 96 tests, Quantitative

400-195-DGT 1 Kit
EUR 854.4

Mouse Clusterin ELISA kit 96 tests, Quantitative

610-100-MCL 1 Kit
EUR 927.6

Dog Haptoglobin ELISA kit, 96 tests, Quantitative

6250-10 1 kit
EUR 927.6

Rat Haptoglobin ELISA kit 96 tests, Quantitative

6250-30 1 Kit
EUR 927.6

Pig Haptoglobin ELISA kit 96 tests, Quantitative

6250-40 1 Kit
EUR 927.6

Cat Haptoglobin ELISA kit 96 tests, Quantitative

6250-50 1 Kit
EUR 927.6

Human Myoglobin ELISA kit 96 tests, Quantitative

600-610-HMY 1 Kit
EUR 1000.8

Mouse Myoglobin ELISA kit 96 tests, Quantitative

600-630-MMY 1 Kit
EUR 1000.8

Monkey Thrombin ELISA Kit, 96 tests, Quantitative

9730 1 Kit
EUR 927.6

Human VEGFR2/KDR ELISA Kit, 96 tests, quantitative

200-860-KDR 1 kit
EUR 1000.8

Protein-G (HCPs) ELISA Kit, 96 tests, Quantitative

800-120-PRG 1 Kit
EUR 927.6

Peroxide Quantitative Assay Kit (Water compatible)

BSP069 250Assays, 250preps
EUR 110.11

Human Angiogenin ELISA Kit, 96 tests, Quantitative

100-160-ANH 1 Kit
EUR 854.4

Monkey Myoglobin ELISA kit 96 tests, Quantitative

600-620-MMY 1 Kit
EUR 1000.8

Rabbit Myoglobin ELISA kit 96 tests, Quantitative

600-650-RMY 1 Kit
EUR 1000.8

Mouse Creatinine ELISA Kit, 96 tests, Quantitative

9255 1 Kit
EUR 927.6

Mouse IL-1 beta ELISA Kit, 96 tests, Quantitative

MIL-01B-150 1 kit
EUR 578.4

Human IL-12 p70 ELISA Kit, 96 tests, Quantitative

HIL-012-125 1 kit
EUR 578.4

Human IL-1 beta ELISA Kit, 96 tests, Quantitative

HIL-01B-140 1 kit
EUR 578.4

Mouse TNF-alpha ELISA Kit, 96 tests, Quantitative

100-210-TNF 1 kit
EUR 578.4

Human TNF-alpha ELISA Kit, 96 tests, Quantitative

100-215-TNH 1 kit
EUR 578.4

Mouse Granzyme B ELISA Kit, 96 tests, Quantitative

MGB-100 1 kit
EUR 607.2

resDetect™ E. coli resDNA Quantitative Kit (qPCR)

OPA-O002 100tests
EUR 1070
Description: Residual host cell DNA refers to fragments of DNA from the host organism that are left behind after a biological process such as the production of a biopharmaceutical product or the cultivation of a cell line. This residual DNA can potentially contaminate the final product and affect its safety and efficacy. In the context of biopharmaceutical production, regulatory agencies such as the FDA and EMA have set limits on the amount of residual host cell DNA that is acceptable in a final product. These limits vary depending on the type of product and the route of administration, and residue host cell DNA quantitative kits are designed to ensure that the final product is safe for human use.

Human Rotavirus IgA(RV IgA) ELISA Kit (Quantitative)

SL2609Hu_1 96 Tests Ask for price

Rabbit Calcitonin ELISA Kit, 96 tests, Quantitative

10550 1 Kit
EUR 927.6

Canine Fibrinogen ELISA Kit, 96 tests, Quantitative

10430 1 Kit
EUR 927.6

3-Nitrotyrosine ELISA Kit, 96 tests, Quantitative

10855 1 Kit
EUR 927.6

Sheep Haptoglobin ELISA Kit, 96 tests, Quantitative

10595 1 Kit
EUR 927.6

Human Aldosterone ELISA Kit, 96 tests, Quantitative

1875 1 kit
EUR 634.8

Mouse Haptoglobin ELISA kit 96 tests, Quantitative

6250 1 kit
EUR 927.6

Horse Haptoglobin ELISA kit 96 tests, Quantitative

6250-20 1 kit
EUR 927.6

Sheep Lactoferrin ELISA Kit, 96 tests, Quantitative

8095 1 kit
EUR 1220.4

Shikari (Q-AFA) Filgrastim Quantitative ELISA kit

FIL-QNS-FIL 1x96-wells test plate
EUR 1217.4
Description: Enzyme Immunoassay for Quantitative detection of Filgrastim in human serum and plasma samples.