Search for viral agents in cerebrospinal fluid in patients with multiple sclerosis using real-time PCR and metagenomics
Multiple sclerosis (MS) is a chronic, immune-mediated demyelinating disease of the central nervous system of unclear etiology, but there is some evidence that viral infections could be responsible for triggering autoimmune mechanisms against myelin. We searched for viral RNA and DNA in cerebrospinal fluid (CSF) of 34 MS patients and 13 controls using RT-PCR/PCR against common neurotropic viruses. In addition, shotgun DNA- and RNA-based metagenomics were done in 13 MS patients and 4 controls. Specific quantitative real-time RT-PCR/PCR testing revealed the presence of viral nucleic acid in seven (20.59%) MS patients and in one (7.69%) control patient. In MS patients the most frequently detected was human herpesvirus type 6 (HHV-6; 3 cases; 8.82%); followed by Epstein-Barr virus (EBV; 2 cases; 5.88%), varicella zoster virus (VZV; 1 case; 2.94%) and Enterovirus (EV; 1 case; 2.94%).
The single identified virus among controls was EBV (7.69%). DNA and RNA metagenomic assays did not identify any known eukaryotic viruses even though three of the analyzed samples were low-level positive by specific quantitative real-time PCR. In conclusion, we detected the presence of Herpesviridae and occasionally Enteroviridae in CSF from patients with MS but their prevalence was not significantly higher than among controls. Metagenomic analysis seems to be less sensitive than real-time RT-PCR/PCR and it did not detect any potential viral pathogens.
![]() Pneumocystis carinii antibody |
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10R-2449 | Fitzgerald | 100 ug | EUR 391.00 |
Description: Mouse monoclonal Pneumocystis carinii antibody |
![]() Pneumocystis jiroveci PCR kit |
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PCR-H440-48D | Bioingentech | 50T | EUR 425.80 |
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Description: An conventional PCR kit for detection of Pneumocystis jiroveci |
![]() Pneumocystis jiroveci PCR kit |
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PCR-H440-96D | Bioingentech | 100T | EUR 521.50 |
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Description: An conventional PCR kit for detection of Pneumocystis jiroveci |
![]() MDV Real-Time PCR Kit |
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PD65-06 | Bionote | 96t | EUR 1553.60 |
Description: Please check the datasheet of MDV Real-Time PCR Kit before using the test. |
![]() ALV Real-Time PCR Kit |
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PD65-15 | Bionote | 96t | EUR 1724.40 |
Description: Please check the datasheet of ALV Real-Time PCR Kit before using the test. |
![]() Anti-Human Pneumocystis Carinii antibody |
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STJ16100417 | St John's Laboratory | 1 mL | EUR 747.00 |
![]() 0.2ML CLEAR STRIP CAPS FOR REAL TIME PCR |
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PCR-2CP-RT-C | CORNING | 125/pk | EUR 223.00 |
Description: PCR Plates & Tubes; PCR Strip Tubes and Strip Caps - Axygen |
![]() hsa-mir-521 Real-time RT-PCR Detection Kit |
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20-abx097600 | Abbexa |
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![]() dsGreen for Real-Time PCR, 100×, 50 mL |
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91010 | lumiprobe | 50 mL | EUR 3169.00 |
![]() dsGreen for Real-Time PCR, 100×, 10 mL |
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81010 | lumiprobe | 10 mL | EUR 869.00 |
![]() dsGreen for Real-Time PCR, 100×, 5 mL |
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71010 | lumiprobe | 5 mL | EUR 625.00 |
![]() dsGreen for Real-Time PCR, 100×, 1.5 mL |
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51010 | lumiprobe | 1.5 mL | EUR 331.00 |
![]() dsGreen for Real-Time PCR, 100×, 1 mL |
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41010 | lumiprobe | 1 mL | EUR 249.00 |
![]() dsGreen for Real-Time PCR, 100×, 100 uL |
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11010 | lumiprobe | 100 uL | EUR 59.00 |
![]() dsGreen for Real-Time PCR, 100×, 2 mL |
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61010 | lumiprobe | 2 mL | EUR 363.00 |
![]() Pneumocystis jiroveci RT PCR kit |
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RTq-H440-100D | Bioingentech | 100T | EUR 628.50 |
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Description: A Real-Time PCR kit for detection of Pneumocystis jiroveci . |
![]() Pneumocystis jiroveci RT PCR kit |
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RTq-H440-150D | Bioingentech | 150T | EUR 701.00 |
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Description: A Real-Time PCR kit for detection of Pneumocystis jiroveci . |
![]() Pneumocystis jiroveci RT PCR kit |
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RTq-H440-50D | Bioingentech | 50T | EUR 532.80 |
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Description: A Real-Time PCR kit for detection of Pneumocystis jiroveci . |
![]() PMA Real-Time PCR Bacterial Viability Kit - Salmonella enterica (invA) |
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31033 | Biotium | 1kit | EUR 428.00 |
Description: Minimum order quantity: 1 unit of 1kit |
![]() PMA Real-Time PCR Bacterial Viability Kit - Mycobacterium tuberculosis (groEL2) |
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31034 | Biotium | 1kit | EUR 399.00 |
Description: Minimum order quantity: 1 unit of 1kit |
![]() PMA Real-Time PCR Bacterial Viability Kit - Staphylococcus aureus (nuc) |
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31035 | Biotium | 1kit | EUR 399.00 |
Description: Minimum order quantity: 1 unit of 1kit |
![]() PMA Real-Time PCR Bacterial Viability Kit - Staphylococcus aureus (mecA) |
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31036 | Biotium | 1kit | EUR 399.00 |
Description: Minimum order quantity: 1 unit of 1kit |
![]() PMA Real-Time PCR Bacterial Viability Kit - E. coli (uidA) |
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31050 | Biotium | 1kit | EUR 428.00 |
Description: Minimum order quantity: 1 unit of 1kit |
![]() PMA Real-Time PCR Bacterial Viability Kit - Listeria monocytogenes (hly) |
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31051 | Biotium | 1kit | EUR 399.00 |
Description: Minimum order quantity: 1 unit of 1kit |
![]() PMA Real-Time PCR Bacterial Viability Kit - Legionella pneumophila (mip) |
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31053 | Biotium | 1kit | EUR 428.00 |
Description: Minimum order quantity: 1 unit of 1kit |
![]() Pneumocystis jiroveci One-Step PCR kit |
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Oneq-H440-100D | Bioingentech | 100T | EUR 747.40 |
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Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Pneumocystis jiroveci. |
![]() Pneumocystis jiroveci One-Step PCR kit |
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Oneq-H440-150D | Bioingentech | 150T | EUR 838.75 |
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Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Pneumocystis jiroveci. |
![]() Pneumocystis jiroveci One-Step PCR kit |
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Oneq-H440-50D | Bioingentech | 50T | EUR 628.50 |
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Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Pneumocystis jiroveci. |
![]() PMA Real-Time PCR Bacterial Viability Kit - Salmonella enterica (invA) PMAxx |
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31033-X | Biotium | 1kit | EUR 428.00 |
Description: Minimum order quantity: 1 unit of 1kit |
![]() PMA Real-Time PCR Bacterial Viability Kit - E. coli (uidA) PMAxx |
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31050-X | Biotium | 1kit | EUR 428.00 |
Description: Minimum order quantity: 1 unit of 1kit |
![]() PMA Real-Time PCR Bacterial Viability Kit - Listeria monocytogenes (hly) PMAxx |
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31051-X | Biotium | 1kit | EUR 428.00 |
Description: Minimum order quantity: 1 unit of 1kit |
![]() Novel Coronavirus COVID-19 (2019-nCoV) Real Time RT-PCR Kit |
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RR-0478-02 | Liferiver | 25 tests/kit | EUR 991.00 |
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Description: Novel Coronavirus (2019-nCoV) Real Time RT-PCR Kit is used for the qualitative detection of a novel coronavirus, which was identified in 2019 at Wuhan City, Hubei Province, China, in upper respiratory tract specimens (nasopharyngeal extracts, deep cough sputum, etc.) and lower respiratory tract specimens (alveoli irrigation fluid, etc.) by real time PCR systems. |
![]() hsa-mir-521 Real-Time RT-PCR Detection and U6 Calibration Kit |
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20-abx097601 | Abbexa |
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![]() PMA Real-Time PCR Bacterial Viability Kit - E. coli O157:H7 (Z3276) |
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31037 | Biotium | 1kit | EUR 428.00 |
Description: Minimum order quantity: 1 unit of 1kit |
![]() Human Real-time PCR DNA Quantitation after QPCR DNA Damage Analysis Kit |
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DD2H | Detroit R&D | 1 Kit | EUR 430.00 |
![]() Mouse Real-time PCR DNA Quantitation after QPCR DNA Damage Analysis Kit |
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DD2M | Detroit R&D | 1 Kit | EUR 430.00 |
![]() Rat Real-time PCR DNA Quantitation after QPCR DNA Damage Analysis Kit |
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DD2R | Detroit R&D | 1 Kit | EUR 430.00 |
![]() PCR Mycoplasma Detection Kit |
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M034-Kit | TOKU-E | Kit | EUR 266.00 |
![]() Human Lung Cancer PCR Primer Library |
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HLUCPL-I | Real Time Primers | 1 set | EUR 548.00 |
![]() Human Schwann Cell PCR Primer Library |
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HSCH-I | Real Time Primers | 1 set | EUR 548.00 |
![]() qPCR plate-foil universal (for Real-time PCR, Light Cycler 480) |
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A26979 | GeneOn | 1x100 pcs | EUR 186.00 |
![]() Eztime Real - time PCRPremix 2x,Taqman |
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FYT105-100P | Yeastern Biotech | 100 Preps 1.25 ml | Ask for price |
![]() PMA Real-Time PCR Bacterial Viability Kit - E. coli O157:H7 (Z3276) PMAxx |
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31037-X | Biotium | 1kit | EUR 428.00 |
Description: Minimum order quantity: 1 unit of 1kit |
![]() Pneumocystis Stain Kit |
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K1434-125 | Biovision | EUR 425.00 |
![]() Pneumocystis Stain Kit |
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K1434-30 | Biovision | EUR 316.00 |
![]() Pneumocystis Stain Kit |
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PCS-1 | ScyTek Laboratories | 1 kit(s) | EUR 156.00 |
![]() Pneumocystis Stain Kit |
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PCS-2 | ScyTek Laboratories | 100 Slides | EUR 104.00 |
![]() Eztime Real - time PCRPremix 2x,SYBR Green |
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FYT103-100P | Yeastern Biotech | 100 Preps 1.25 ml | Ask for price |
![]() Eztime Real - time PCRPremix 2x,SYBR Green |
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FYT103-400P | Yeastern Biotech | 400 Preps 1.25 ml x 4 | Ask for price |
![]() Eztime Real - time PCRPremix 2x,SYBRGreen , ROX |
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FYT104-100P | Yeastern Biotech | 100 Preps 1.25 ml | Ask for price |
![]() Eztime Real - time PCRPremix 2x,SYBRGreen , ROX |
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FYT104-400P | Yeastern Biotech | 400 Preps 1.25 ml x 4 | Ask for price |
![]() Eztime Real - time PCRPremix 2x,Taqman, ROX |
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FYT105-400P | Yeastern Biotech | 400 Preps 1.25 ml x 4 | Ask for price |
![]() Eztime Real - time PCRPremix 2x,Taqman, ROX |
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FYT106-100P | Yeastern Biotech | 100 Preps 1.25 ml | Ask for price |
![]() 0,1ML THIN WALL TUBES 8 PER STRIP. CLEAR & REAL TIME STRIP CAPS |
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PCR-0108-LP-RT-C | CORNING | 125/pk | EUR 709.00 |
Description: PCR Plates & Tubes; PCR Strip Tubes and Strip Caps - Axygen |
![]() 0,1ML THIN WALL TUBES 8 PER STRIP. WHITE & REAL TIME STRIP CAPS |
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PCR-0108-LP-RT-W | CORNING | 125/pk | EUR 770.00 |
Description: PCR Plates & Tubes; PCR Strip Tubes and Strip Caps - Axygen |
![]() Green Dye One Step qRT-PCR Master Mix For Quantitative Real Time PCR With ROX Dye |
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MB1004-100Reactions | BosterBio | 100 Reactions | EUR 327.00 |
![]() hsa-mir-34a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097657 | Abbexa |
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![]() hsa-mir-34b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097659 | Abbexa |
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![]() hsa-mir-92a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097661 | Abbexa |
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![]() hsa-mir-92b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097662 | Abbexa |
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![]() hsa-mir-93 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097663 | Abbexa |
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![]() hsa-mir-95 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097664 | Abbexa |
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![]() hsa-mir-96 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097665 | Abbexa |
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![]() hsa-mir-98 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097666 | Abbexa |
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![]() hsa-mir-99a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097667 | Abbexa |
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![]() hsa-mir-99b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097669 | Abbexa |
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![]() hsa-mir-100 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097671 | Abbexa |
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![]() hsa-mir-101 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097673 | Abbexa |
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![]() hsa-mir-103 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097675 | Abbexa |
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![]() hsa-mir-105 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097676 | Abbexa |
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![]() hsa-mir-106a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097677 | Abbexa |
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![]() hsa-mir-106b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097678 | Abbexa |
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![]() hsa-mir-107 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097679 | Abbexa |
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![]() hsa-mir-124 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097680 | Abbexa |
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![]() hsa-mir-125a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097681 | Abbexa |
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![]() hsa-mir-127 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097686 | Abbexa |
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![]() hsa-mir-129 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097689 | Abbexa |
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![]() hsa-mir-130a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097690 | Abbexa |
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![]() hsa-mir-130b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097691 | Abbexa |
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![]() hsa-mir-132 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097692 | Abbexa |
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![]() hsa-mir-133a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097693 | Abbexa |
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![]() hsa-mir-133b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097694 | Abbexa |
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![]() hsa-mir-134 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097695 | Abbexa |
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![]() hsa-mir-135b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097696 | Abbexa |
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![]() hsa-mir-137 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097699 | Abbexa |
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![]() hsa-mir-138 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097700 | Abbexa |
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![]() hsa-mir-139 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097701 | Abbexa |
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![]() hsa-mir-140 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097702 | Abbexa |
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![]() hsa-mir-141 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097703 | Abbexa |
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![]() hsa-mir-143 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097706 | Abbexa |
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![]() hsa-mir-145 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097708 | Abbexa |
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![]() hsa-mir-146a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097709 | Abbexa |
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![]() hsa-mir-149 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097711 | Abbexa |
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![]() hsa-mir-150 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097712 | Abbexa |
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![]() hsa-mir-151 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097714 | Abbexa |
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![]() hsa-mir-152 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097715 | Abbexa |
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![]() hsa-mir-153 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097716 | Abbexa |
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![]() hsa-mir-154 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097717 | Abbexa |
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![]() hsa-mir-181a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097719 | Abbexa |
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![]() hsa-mir-181b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097720 | Abbexa |
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![]() hsa-mir-181c Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097721 | Abbexa |
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![]() hsa-mir-181d Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097722 | Abbexa |
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![]() hsa-mir-182 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097723 | Abbexa |
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![]() hsa-mir-183 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097724 | Abbexa |
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![]() hsa-mir-184 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097725 | Abbexa |
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![]() hsa-mir-185 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097726 | Abbexa |
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![]() hsa-mir-190a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097728 | Abbexa |
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![]() hsa-mir-190b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097729 | Abbexa |
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![]() hsa-mir-192 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097731 | Abbexa |
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![]() hsa-mir-193a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097732 | Abbexa |
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![]() hsa-mir-193b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097733 | Abbexa |
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![]() hsa-mir-194 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097734 | Abbexa |
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![]() hsa-mir-195 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097735 | Abbexa |
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![]() hsa-mir-197 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097738 | Abbexa |
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![]() hsa-mir-198 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097739 | Abbexa |
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![]() hsa-mir-199a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097740 | Abbexa |
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![]() hsa-mir-199b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097741 | Abbexa |
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![]() hsa-mir-200a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097742 | Abbexa |
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![]() hsa-mir-200b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097743 | Abbexa |
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![]() hsa-mir-202 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097745 | Abbexa |
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![]() hsa-mir-203 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097746 | Abbexa |
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![]() hsa-mir-204 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097747 | Abbexa |
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![]() hsa-mir-205 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097748 | Abbexa |
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![]() hsa-mir-206 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097749 | Abbexa |
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![]() hsa-mir-208a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097750 | Abbexa |
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![]() hsa-mir-208b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097751 | Abbexa |
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Genetic Distances of Rainbow Trout and Masu Salmon as Determined by PCR-Based Analysis
This study used a PCR-based genetic analysis platform to create a hierarchical polar dendrogram of Euclidean genetic distances for two salmonid species, Oncorhynchus mykiss (rainbow trout, RT) and Oncorhynchus masou (masu salmon, MS). The species were distantly related to other fish species based on PCR results from using the designed oligonucleotide primer series. Five oligonucleotide primers were used to generate 330 and 234 scorable fragments in the RT and MS populations, respectively. The DNA fragments ranged in size from approximately 50 bp to more than 2,000 bp.

mhealthinfo
The bandsharing (BS) results showed that the RT population had a higher average BS value (0.852) than that for the MS population (0.704). The genetic distance between individuals supported the presence of adjacent affiliation in cluster I (RT 01-RT 11). The observation of a significant genetic distance between the two Oncorhynchus species verifies that this PCR-based technique can be a useful approach for individual- and population-based biological DNA investigations. The results of this type of investigation can be useful for species safekeeping and the maintenance of salmonid populations in the mountain streams of Korea.
Multiplex real-time PCR using double-strand primers and probes for the detection of nucleic acids
Multiplex PCR encounters difficulties in primer designing with all the primer pairs working at the same annealing temperature. In this study, we have developed a double-strand primer-mediated multiple strand displacement reaction for the detection of SARS-COV-2 ORF, N and E genes (as examples). The double primer is composed of a 5′-modified fluorophore strand, which does not impact polymerase extension and a 3′-modified quencher strand, which cannot impact elongation. At the annealing temperature, the fluorophore strand combined with the template, extended and resulted in fluorescence signal release.
Results showed that the double-strand primer relatively exhibits a wide annealing temperature range and good compatibility between three pairs of primers and probes. These merits allow the simple and multiplex real-time fluorescence quantification of nucleic acids. The detection limit was 400 copies/mL, and the detection time was approximately 2 h. In addition to its extreme specificity and simplicity, this method has a wide range of applications such as multiple PCR and SNP detection.
Neonatal Acute Lymphoblastic Leukemia with t(9;11) Translocation Presenting as Blueberry Muffin Baby: Successful Treatment by ALL-BFM Induction Therapy, Allogeneic Stem Cell Transplantation from an Unrelated Donor, and PCR-MRD-Guided Post-Transplant Follow-Up
BACKGROUND Neonatal acute leukemia is a rare condition. Little is known about its incidence and outcomes, and treatment options have not been standardized. CASE REPORT A 3-day old, apparently healthy male newborn was referred to the pediatric intensive care unit with multiple violaceous macules and a few papules on his face and upper trunk. After initial spontaneous regression, the lesions reappeared. Skin biopsy and bone marrow aspirate revealed a diagnosis of acute lymphoblastic leukemia (ALL). ALL induction therapy was initiated on day 24, resulting in morphological remission at the end of induction therapy. ALL chemotherapy was guided by sequential PCR-based monitoring of minimal residual disease (MRD).
The patient received a transplant from an unrelated HLA high-resolution matched (10/10 loci) permissive donor. He was followed-up after transplant conducted by sequential PCR-based measurements of MRD in bone marrow. CONCLUSIONS Neonatal leukemia often presents as congenital skin lesions known as blueberry muffin rash. ALL induction therapy was started at the end of the neonatal period. Treatment was well-tolerated and effective. Early donor search and PCR-MRD guided treatment surveillance can help to achieve and maintain molecular remission.
Sex determination from the pulp tissue of deciduous teeth exposed to natural soil and wet clay – A PCR study
Context: Dental tissue remains are the toughest, and chemically, the most stable tissue in the body. Its high resilience in the events of fire and bacterial decomposition makes them vital for DNA analysis by PCR method.
Aims: Determination of sex of children through molecular analysis of pulp tissue of exfoliated deciduous teeth stored in different media and analyzed after a different time period.
Settings and design: Sixty samples of deciduous teeth were divided into three groups. Group IA and Group IIA were stored in natural soil and wet clay for 1 month, respectively. Group IB and Group IIB were stored in natural soil and wet clay for 6 months, respectively. Group III was analyzed immediately after extraction.
Methods and material: Sex determination was carried out in five steps: Pulp tissue removal, DNA isolation, DNA quantification, PCR amplification, Sex determination. X and Y specific chromosomes from each sample were amplified and compared.
Statistical analysis used: Kruskal-Wallis test, Dunn’s test, and Wilcoxon signed rank test.
Results: Group III revealed the highest amount of DNA quantified. Amount of DNA quantified after 6 months of storage in natural soil and wet clay decreased in both the groups with the samples stored in wet clay showing a maximum decrease. Results of the PCR analysis also showed 100% accuracy rate in the samples of Group III.
Conclusions: Sex determination from pulp tissue depends a lot on the quality and quantity of DNA extracted. Sex could be effectively determined among the samples evaluated immediately after extraction. This ability decreases as the storage condition changes and the time period increases. Samples stored in wet clay were found to show the least sex identification ability than dry soil.
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81010 | lumiprobe | 10 mL | EUR 869.00 |
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71010 | lumiprobe | 5 mL | EUR 625.00 |
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51010 | lumiprobe | 1.5 mL | EUR 331.00 |
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41010 | lumiprobe | 1 mL | EUR 249.00 |
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11010 | lumiprobe | 100 uL | EUR 59.00 |
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61010 | lumiprobe | 2 mL | EUR 363.00 |
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MB1004-100Reactions | BosterBio | 100 Reactions | EUR 327.00 |
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31033 | Biotium | 1kit | EUR 428.00 |
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31034 | Biotium | 1kit | EUR 399.00 |
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31035 | Biotium | 1kit | EUR 399.00 |
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31036 | Biotium | 1kit | EUR 399.00 |
Description: Minimum order quantity: 1 unit of 1kit |
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31050 | Biotium | 1kit | EUR 428.00 |
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31051 | Biotium | 1kit | EUR 399.00 |
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31053 | Biotium | 1kit | EUR 428.00 |
Description: Minimum order quantity: 1 unit of 1kit |
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31033-X | Biotium | 1kit | EUR 428.00 |
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31050-X | Biotium | 1kit | EUR 428.00 |
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31051-X | Biotium | 1kit | EUR 428.00 |
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RR-0478-02 | Liferiver | 25 tests/kit | EUR 991.00 |
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Description: Novel Coronavirus (2019-nCoV) Real Time RT-PCR Kit is used for the qualitative detection of a novel coronavirus, which was identified in 2019 at Wuhan City, Hubei Province, China, in upper respiratory tract specimens (nasopharyngeal extracts, deep cough sputum, etc.) and lower respiratory tract specimens (alveoli irrigation fluid, etc.) by real time PCR systems. |
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20-abx097601 | Abbexa |
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31037 | Biotium | 1kit | EUR 428.00 |
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DD2H | Detroit R&D | 1 Kit | EUR 430.00 |
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M034-Kit | TOKU-E | Kit | EUR 266.00 |
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HLUCPL-I | Real Time Primers | 1 set | EUR 548.00 |
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31037-X | Biotium | 1kit | EUR 428.00 |
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PCR-0108-LP-RT-C | CORNING | 125/pk | EUR 709.00 |
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PCR-0108-LP-RT-W | CORNING | 125/pk | EUR 770.00 |
Description: PCR Plates & Tubes; PCR Strip Tubes and Strip Caps - Axygen |
![]() hsa-mir-34a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097657 | Abbexa |
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20-abx097776 | Abbexa |
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20-abx097777 | Abbexa |
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20-abx097786 | Abbexa |
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